Amit Gur

Amit Gur
Agricultural Research Organization ARO | aro · Institute of Plant Sciences

PhD

About

61
Publications
13,457
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2,605
Citations
Additional affiliations
December 2004 - October 2007
Cornell University
Position
  • PostDoc Position
October 1999 - October 2004
Hebrew University of Jerusalem
Position
  • PhD Student

Publications

Publications (61)
Article
Macrophomina phaseolina is a soil-borne fungal pathogen that incites charcoal rot in more than 500 plant species including melon, Cucumis melo. Disease incidence and severity are affected by host genetic background, plant age, temperature, and water economy. The non-genetic variation in disease severity exhibited by infected melon plants poses a ch...
Article
Full-text available
The plants of summer squash, Cucurbita pepo, are monoecious, bearing their flowers in leaf axils. Plants of C. pepo subsp. pepo, which includes Cocozelle and Zucchini squash, bear one flower per leaf axil whilst plants of C. pepo subsp. texana, which includes Crookneck squash, differentiate two or more flower buds at nearly all leaf axils. This mul...
Article
Earliness and ripening behavior are important attributes of fruits on and off the vine, and affect quality and preference of both growers and consumers. Fruit ripening is a complex physiological process that involves metabolic shifts affecting fruit color, firmness, and aroma production. Melon is a promising model crop for the study of fruit ripeni...
Article
Heterosis, the superiority of hybrids over their parents, is a major genetic force associated with plant fitness and crop yield enhancement. Understanding and predicting heterosis is crucial for evolutionary biology, as well as for plant and animal breeding. We investigated root-mediated yield heterosis in melons (Cucumis melo) by characterizing co...
Preprint
Full-text available
Heterosis, the superiority of hybrids over their parents, is a major genetic force associated with plant fitness and crop yield enhancement. Understanding and predicting heterosis is crucial for evolutionary biology, as well as for plant and animal breeding. We investigated root-mediated yield heterosis in melons ( Cucumis melo ) by characterizing...
Article
Full-text available
Melon is an important crop that exhibits broad variation for fruit morphology traits that are the substrate for genetic mapping efforts. In the post-genomic era, the link between genetic maps and physical genome assemblies is key for leveraging QTL mapping results for gene cloning and breeding purposes. Here, using a population of 164 melon recombi...
Article
Full-text available
The broad variability of Cucumis melo (melon, Cucurbitaceae) presents a challenge to conventional classification and organization within the species. To shed further light on the infraspecific relationships within C. melo, we compared genotypic and metabolomic similarities among 44 accessions representative of most of the cultivar-groups. Genotypin...
Preprint
Full-text available
During climacteric ripening large-scale transcriptional modifications are governed by ethylene. While ripening-related chromatin modifications are also known to occur, a direct connection between these factors has not been demonstrated. We characterized ethylene-mediated transcriptome modification, genome methylation dynamics, and their relation to...
Article
Full-text available
Melon is an economically important fruit crop that has been cultivated for thousands of years; however, the genetic basis and history of its domestication still remain largely unknown. Here we report a comprehensive map of the genomic variation in melon derived from the resequencing of 1,175 accessions, which represent the global diversity of the s...
Article
Full-text available
Carotenoids have various roles in plant physiology. Plant carotenoids are synthesized in plastids and are highly abundant in the chromoplasts of ripening fleshy fruits. Considerable research efforts have been devoted to elucidating mechanisms that regulate carotenoid biosynthesis, yet, little is known about the mechanism that triggers storage capac...
Article
Full-text available
Color and pigment contents are important aspects of fruit quality and consumer acceptance of cucurbit crops. Here, we describe the independent mapping and cloning of a common causative APRR2 gene regulating pigment accumulation in melon and watermelon. We initially show that the APRR2 transcription factor is causative for the qualitative difference...
Article
Full-text available
Years of selection for desirable fruit quality traits in dessert watermelon (Citrullus lanatus) has resulted in a narrow genetic base in modern cultivars. Development of novel genomic and genetic resources offers great potential to expand genetic diversity and improve important traits in watermelon. Here we report a high‐quality genome sequence of...
Preprint
Full-text available
Color and pigment content are important aspects of fruit quality and consumer acceptance of cucurbit crops. Here, we describe the independent mapping and cloning of a common causative APRR2 gene regulating pigment accumulation in melon and watermelon. We initially show that the APRR2 transcription factor is causative for the qualitative difference...
Article
Full-text available
Combined quantitative trait loci (QTL) and expression-QTL (eQTL) mapping analysis was performed to identify genetic factors affecting melon (Cucumis melo) fruit quality, by linking genotypic, metabolic and transcriptomic data from a melon recombinant inbred line (RIL) population. RNA sequencing (RNA-Seq) of fruit from 96 RILs yielded a highly satur...
Article
Full-text available
Cucumis melo is highly diverse for fruit traits providing wide breeding and genetic research opportunities, including genome-wide association (GWA) analysis. We used a collection of 177 accessions representing the two C. melo subspecies and 11 horticultural groups for detailed characterization of fruit traits variation and evaluation of the potenti...
Article
Full-text available
The common flesh color of commercially grown watermelon is red due to the accumulation of lycopene. However, natural variation in carotenoid composition that exists among heirloom and exotic accessions results in a wide spectrum of flesh colors. We previously identified a unique orange flesh watermelon accession (NY0016) that accumulates mainly β-c...
Article
High resolution QTL mapping of genetic factors affecting fruit quality traits in melon (Cucumis melo) was achieved in two projects. Next-generation sequencing (NGS), coupled with unique genetic materials, was used to attain high resolution mapping, in both studies. The first project was based on the RNA-seq of mature fruits of a Recombinant Inbred...
Chapter
Full-text available
Fruit quality in melon (Cucumis melo) and in other cucurbit species is primarily determined by sweetness, acidity, aroma, color and shelf-life. During ripening, the mesocarp (fruit flesh), the consumed tissue, generally softens due to degradation of cell walls, and accumulates soluble sugars, organic acids, volatiles and additional secondary metabo...
Article
Full-text available
To investigate the natural variability of leaf metabolism and enzymatic activity in a maize inbred population, statistical and network analyses were employed on metabolite and enzyme profiles. The test of coefficient of variation showed that sugars and amino acids displayed opposite trends in their variance within the population, consistently with...
Data
Metabolite and enzyme BLUP profiles.
Data
Coefficient of variation of metabolites and enzymes.
Data
Broad-sense heritability values of enzymes.
Data
Correlation coefficients and r2 values of Pearson correlation.
Data
Correlation-based metabolite network with communities. Network visualization of metabolites as analyzed on the IBM population. Metabolites are presented as nodes and their relations as edges. The Spearman rank correlation was employed to compute all pairwise correlations between metabolites across the entire set of inbred lines. Solely significant...
Data
Broad-sense heritability values of metabolites.
Article
Full-text available
Molecular markers allowed breeders to mendelize quantitative trait loci (QTL) providing another demonstration that quantitative traits are governed by the same principles as single qualitative genes. This research extends the QTL analysis to two and three QTL and tests our ability to mendelize an oligogenic trait. In tomato, agricultural yield is d...
Data
Supplementary Table 1. Phenotypic effects and mode of inheritance of significant IL-QTL for the six measured traits in the wet (A) and dry (B) fields. Phenotypic effects of ILs and ILHs are presented as percentage difference from M82. Values that are significantly different from M82, at P < 0.01, are in bold. Mode of inheritance index is the mode o...
Data
Supplementary Fig. 1. Soil water content in the wet and dry fields in Akko. Soil moisture at different times during the growing season and at different depths in the Akko 2000 and 2001 trials. A, B and C are measurements of tensiometers in Akko 2000 in the dry and wet treatments at depth points of 30, 60 and 90 cm, respectively. Planting date was 1...
Article
Full-text available
Plant yield is the integrated outcome of processes taking place above and below ground. To explore genetic, environmental and developmental aspects of fruit yield in tomato, we phenotyped an introgression line (IL) population derived from a cross between the cultivated tomato (Solanum lycopersicum) and a wild species (Solanum pennellii). Both homoz...
Data
Metabolite profiles in red fruits. Data are normalized by the mean of M82 (M82 = 1.00). Values represent the calculated fraction of M82. Data points consist of the mean out of six replicates ± SE; bold type values were determined by the t test to be significantly different (P < 0.05) from M82.
Article
Full-text available
Harvest index, defined as the ratio of reproductive yield to total plant biomass, and early ripening are traits with important agronomic value in processing tomatoes. The Solanum pennellii introgression-line (IL) population shows variation for harvest index and earliness. Most of the QTL mapped for these traits display negative agronomic effects; h...
Article
Full-text available
Understanding the genetic basis of nitrogen and carbon metabolism will accelerate the development of plant varieties with high yield and improved nitrogen use efficiency. A robotized platform was used to measure the activities of 10 enzymes from carbon and nitrogen metabolism in the maize (Zea mays) intermated B73 × Mo17 mapping population, which p...
Data
Activities of nine CCM enzymes for eight different maize inbred lines in five replications. (0.04 MB DOC)
Data
IDH activity at two incubation temperatures across 100 maize inbred-lines. (A) Correlation between IDH activity at 25°C and 35°C. Each point represents the mean of five replications from each inbred line. Parallel to the X and Y axes are the frequency distribution of the activity measurements. (B) Two way ANOVA for IDH activity against line and inc...
Data
Selected CCM enzymes and their pathway location. PGM: phophoglucomutase, HEX: hexokinase, GLK: glucokinase, G6PDH: glucose-6-phosphate dehydrogenase, ALD: fructose-biphosphate aldolase, GAPDH: NADP-dependent glyceraldehyde-3-phosphate dehydrogenase PGK: phosphoglycerate kinase, PGAM: phosphoglycerate mutase, ENO: enolase, ACO: aconitase, IDH: NAD-d...
Data
EMBL/GenBank accession numbers. (0.03 MB DOC)
Article
Full-text available
Central carbon metabolism (CCM) is a fundamental component of life. The participating genes and enzymes are thought to be structurally and functionally conserved across and within species. Association mapping utilizes a rich history of mutation and recombination to achieve high resolution mapping. Therefore, applying association mapping in maize (Z...
Article
Full-text available
Heterosis, or hybrid vigor, is a major genetic force that contributes to world food production. The genetic basis of heterosis is not clear, and the importance of loci with overdominant (ODO) effects is debated. One problem has been the use of whole-genome segregating populations, where interactions often mask the effects of individual loci. To ass...
Article
Fruit size and shape are among the most important traits that were under selection during domestication of the fruit-bearing Solanaceae crops, tomato and pepper. To determine the level of conservation of the locations of quantitative trait locus (QTL) controlling these traits in the two species, we conducted whole genome comparative QTL analysis fo...
Article
Full-text available
Tomato represents an important source of fiber and nutrients in the human diet and is a central model for the study of fruit biology. To identify components of fruit metabolic composition, here we have phenotyped tomato introgression lines (ILs) containing chromosome segments of a wild species in the genetic background of a cultivated variety. Usin...
Article
We describe here a novel plant-specific gene, Lefsm1 (fruit SANT/MYB-like 1) harboring a single SANT/MYB domain. The expression of Lefsm1 is specific to the very early stages of tomato (Lycopersicon esculentum) fruit development. Ectopic expression of Lefsm1 results in severe developmental alterations manifested in retarded growth, and reduced apic...
Article
Full-text available
Natural biodiversity is an underexploited sustainable resource that can enrich the genetic basis of cultivated plants with novel alleles that improve productivity and adaptation. We evaluated the progress in breeding for increased tomato (Solanum lycopersicum) yield using genotypes carrying a pyramid of three independent yield-promoting genomic reg...
Article
Full-text available
In order to screen for putative candidate genes linked to tomato fruit weight and to sugar or acid content, genes and QTLs involved in fruit size and composition were mapped. Genes were selected among EST clones in the TIGR tomato EST database (http://www.tigr.org/tdb/tgi/lgi/) or corresponded to genes preferentially expressed in the early stages o...
Article
A current bioinformatic challenge is to include information in genomic databases about subtle phenotypic differences that show continuous distribution in segregating populations. Real Time quantitative trait loci (QTL) present, in silico, a range of statistical and graphical outputs that describe components of the genetic variation using wild speci...
Article
Determining gene sequences responsible for complex phenotypes has remained a major objective in modern biology. The candidate gene approach is attempting to link, through mapping analysis, sequences that have a known functional role in the measured phenotype with quantitative trait loci (QTL) that are responsible for the studied variation. To explo...
Article
Vegetable cultivation favored the inclusion of pleasant aromas in the produce, whereas unpleasant aromas were selected against. Introgression lines, generated by hybridization of a cultivated tomato (Lycopersicon esculentum) to its wild relative L. pennellii, were used to map quantitative trait loci (QTL) that influence tomato aroma. A marked undes...
Article
Full-text available
Congenic lines that differ in a single defined chromosome segment are useful for the study of complex phenotypes, as they allow isolation of the effect of a particular quantitative trait locus (QTL) from those of the entire genome. We conducted high-resolution QTL mapping of a 9-cM introgression, originating from the wild tomato species Lycopersico...

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