Amir Reza Jassbi

Shiraz University of Medical Sciences | SUMS · Medicinal and Natural Products Chemistry Research Center

Phytochemical investigation of the chloroform extract of Scilla bisotunensis Speta. bulbs led to the isolation and structure elucidation of six sappinane-4-one (1-4, 7, 9) and three 3,9-dehydrosappinan-4-one (5, 6, 8) homoisoflavonoids. The structures of compounds 1-9 were established based on extensive NMR, ESIMS, UV, and ECD spectra, and also by...

Background: Salvia extracts have various biological activities and are rich sources of bioactive metabolites. Objectives: We identified five phytochemicals from S. compressa extract and assessed its biological properties. Methods: The plant's shoots were extracted using dichloromethane, and the constituents were isolated using column chromatog...

Background: Brown algae have gained worldwide attention due to their significant biological activities, such as antidiabetic properties. In the present study, the antidiabetic properties of six brown algae from the Persian Gulf were investigated. Methods: An experimental study was conducted from 2017 to 2019 to examine the inhibitory effects of...

Background: Ursane triterpenoids could be considered as novel multi-target therapeutic anti-cancer agents. Salvurmin A and Salvurmin B are novel cytotoxic ursane triterpenoids isolated from the aerial parts of Salvia urmiensis, an endemic plant species of Iran. The isolation and structure elucidation were reported in our recent publication. Methods...

Salvia grossheimii is a perennial herb with antidiabetic and cytotoxic constituents. In continuation of our study on S. grosshiemii to identify the bioactive phytochemicals, we have reported the characterization of seven undescribed triterpenoids. The aerial parts of the plant were extracted in dichloromethane and its constituents were isolated usi...

Context Ircinia mutans Wilson (Irciniidae) is a sponge with antimicrobial and cytotoxic constituents. Objective Our objective was to characterise the cytotoxic constituents of two seasonal collections of I. mutans. Materials and methods The sponges were extracted in methanol-dichloromethane and their constituents were purified and characterised u...

Moluccella aucheri Scheen belongs to Lamiaceae family and is native to Iran and Pakistan. Different chromatographic and spectroscopic analyses of a dichloromethane extract of M. aucheri's aerial parts led to the isolation of five phytochemicals. They include an undescribed labdane type- diterpene; 6ß -acetoxy-9α-hydroxy-7- oxa- labd-13- ene- 15, 16...

Bioassay-guided fractionation of a dichloromethane extract of the aerial parts of Salvia urmiensis, an endemic plant species of Iran, led to the isolation of two new cytotoxic ursane triterpenoids, Salvurmin A and Salvurmin B. Their structures were elucidated by a combination of 1D and 2D NMR, HR-ESI-MS, IR and UV analysis. Cytotoxicity of the abov...

Marine sources have attracted worldwide attention due to their various biological activities. This study reports the cytotoxic, antioxidant, and antibacterial activities and phenolic contents of solvent extracts sequentially prepared by 80% methanol (MeOH), MeOH, and dichloromethane (DCM), of five brown algae from the Persian Gulf: Colpomenia sinuo...

The roots of Salvia spinosa L. (Lamiaceae) were extracted with hexane, dichloromethane (DCM) and ethyl acetate. The DCM extract exhibited cytotoxic activity (IC50 32.7 µg/mL) against MFC-7 breast cancer cell line in MTT colorimetric bioassay. Ferruginol (1), taxodione (2), 12-deoxy-6-hydroxy-6,7-dehydroroyleanone (3), 14-deoxycoleon U (4), 15-deoxy...

Cyanobacteria are photosynthetic prokaryotic organisms which represent a significant source of novel, bioactive, secondary metabolites, and they are also considered an abundant source of bioactive compounds/drugs, such as dolastatin, cryptophycin 1, curacin toyocamycin, phytoalexin, cyanovirin-N and phycocyanin. Some of these compounds have display...

Cyanobacteria are photosynthetic prokaryotic organisms which represent a significantsource of novel, bioactive, secondary metabolites, and they are also considered an abundant source ofbioactive compounds/drugs, such as dolastatin, cryptophycin 1, curacin toyocamycin, phytoalexin,cyanovirin-N and phycocyanin. Some of these compounds have displayed...

The outbreak of diseases caused by Candida species is growing. These opportunistic pathogens can produce biofilm on non-biological surfaces, such as removable orthodontic appliances. Over the past two decades, there has been a significant increase in resistance to several types of antifungal drugs. Recently, there is a growing interest in using her...

Three undescribed dammarane-type saponins, russelliinosides A-C, together with a common sterol (β-sitosterol), an abietane diterpenoid (18-hydroxyferruginol), two oleane triterpenoids (daturaolone and oleanolic acid), an ursane triterpenoid (ursolic acid) as well as three 5-hydroxyflavones (cirsimaritin, eupatorin, and salvigenin) were isolated fro...

Glycogen storage diseases (GSDs) are known as complex disorders with overlapping manifestations. These features also preclude a specific clinical diagnosis, requiring more accurate paraclinical tests. To evaluate the patients with particular diagnosis features characterizing GSD, an observational retrospective case study was designed by performing...

The genus Moluccella (Lamiaceae) encompasses eight species among which Moluccella aucheri (Boiss.) Scheen and M. laevis L. are available in Iran. The aim of this study is characterizing the essential oil of dried aerial parts of M. aucheri collected from the South of Iran. The essential oil was hydrodistilled and then analysed by GC-MS. Twenty-one...

Introduction: Rosa damascena Mill distillate and its essential oil are widely used in cosmetics, perfumes and food industries. Therefore, the methods of detection for its authentication is an important issue. Objectives: We suggest colorimetric sensor array and chemometric methods to discriminate natural Rosa distillate from synthetic adulterate...

Background & Objective: α-Glucosidase is one of the main enzymes in the intestinal absorption of carbohydrates. Inhibition of this enzyme can improve postprandial hyperglycemia in diabetic patients. Also, antioxidants can ameliorate diabetes complications resulted from oxidative stress. In this study α-glucosidase inhibition, antioxidant activity a...

The roots and the shoots of Euphorbia macrostegia and E. microsciadia were extracted using different solvents; dichloromethane (DCM), methanol (MeOH) and MeOH: water (80:20) and the extracts were screened for their cytotoxic, antioxidant and antimicrobial properties as well as total phenolic content. Cytotoxicity of the extracts was evaluated again...

Further investigations on phytochemical constituents of dichloromethane extract from roots of Salvia lachnocalyx (S. lachnocalyx) led to the isolation and identification of eight known diterpenoids from this plant for the first time. The chemical structures of the purified compounds were elucidated using spectroscopic analyses including EI-MS, 1 H...

Iran is one of the main hubs for sage’s diversity in Asia. Generally, prediction and characterization of the chemical structures of the specialized metabolites that have significant role in the bioactivity of a plant remains a significant challenge. In this study, 50 different accessions of 32 Salvia species in 2015 and 2017 were collected and extr...

The marine environment with its vast biological diversity encompasses many organisms that produce bioactive natural products. Marine microorganisms are rich sources of compounds from many structural classes with a multitude of biological activities. The biosynthesis of microbial natural products depends on a variety of biotic and abiotic factors in...

Iran is one of the main hubs for sage’s diversity in Asia. Generally, prediction and characterization of the chemical structures of the specialized metabolites that have significant role in the bioactivity of a plant remains a significant challenge. In this study, 50 different accessions of 32 Salvia species in 2015 and 2017 were collected and extr...

Four abietane diterpenoids, agastanol, (1), 6, 7-dehydroroyleanone (2), 7α-acetoxyroyleanone (3), and ferruginol (4) have isolated from root extract of Salvia hydrangea. The chemical structures of compounds were identified using EI-MS, ESI-MS, 1D, and 2D NMR spectroscopic analyses. Antileishmanial activity of 1- 4 was tested against the promastigot...

Two polyhydroxylated oleanane and seven ursane triterpenoids were isolated from aerial parts of Salvia gros-sheimii. The chemical structures of the undescribed triterpenoids (1-6) were characterized using 1 and 2 D NMR and ESI-MS spectral data as; 2α, 3β, 11α-trihydroxy-olean-12-ene (1), 2α, 3β, 11α-trihydroxy-olean-18-ene (2), 2α-acetoxy-urs-12-en...

Two polyhydroxylated oleanane and seven ursane triterpenoids were isolated from aerial parts of Salvia grossheimii. The chemical structures of the undescribed triterpenoids (1-6) were characterized using 1 and 2 D NMR and ESI-MS spectral data as; 2α, 3β, 11α –trihydroxy-olean-12- ene (1), 2α, 3β, 11α-trihydroxy-olean-18-ene (2), 2α- acetoxy-urs-12-...

MTT colorimetric cytotoxic bioassay, solvent fractionation and chromatographic purification of an 80% methanol extract of Salvia dracocephaloides, led to isolation and identification of eight compounds, including 5-hydroxy-3,6,7,3ʹ,4ʹ-pentamethoxyflavone (1), cynaroside (2), salvigenin (3), eupatorin (4), cirsimaritin (5), β-sitosterol (6), oleanol...

Xanthones, polyphenolic compounds, are one of the most important classes in natural products, as they exhibit variety of pharmacological and health benefits, i.e., anti-obesity, antidiabetic, antioxidant, anti-Alzheimer, anti-inflammatory, and anticancer. Xanthones can be found in higher plants that are used as food sourcesfor example, Garcinia man...

Among specialized metabolites, terpenoids are well-known for their cytotoxic activity. Several of them have been isolated from sage plants and assayed for their potential therapeutic use against cancer. In this study, we report the effects of three potent anticancer terpenoids previously isolated from Salvia lachnocalyx, including geranyl farnesol...

Molecularly imprinted polymers (MIPs) are polymers possessing receptor cavities with selective characteristics. The molecularly imprinted polymer of 1,8-cineole on hydroxyl-functionalized multiwall carbon nanotubes was synthesized for selective extraction of 1,8-cineole in water distillates of Artemisia sieberi (sagebrush) and thyme samples. The MI...

We isolated an actinobacterium, Streptomyces sp. strain SP 85 from the marine sponge Dysidea avara. Polyphasic identification of the microorganism showed that the strain SP 85 had high 16S rRNA gene similarity (99%) with Streptomyces olivaceus strain NBRC 12805, while some physiological and biochemical differences were observed. A cytotoxic compoun...

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytoto...

Poly(N,N′-methylenebisacrylamide) was synthesized for the selective solid-phase extraction (SPE) and determination of artemisinin in ethanolic extract of five different Artemisia species of Iran, including A. annua, A. aucheri, A. vulgare, A. sieberi and A. scoporia using LC–MS. Artemisinin is a natural product which is used as an anti-malarial dru...

Purpose: Marine sponges are rich sources of anticancer metabolites. Axinella sinoxea is a less studied sponge, found in the Larak Island's waters, of the Persian Gulf. In the present study, we have explored the cytotoxic properties and chemical constituents of A. sinoxea. Methods: Repeated silica gel flash column chromatography of methanol extra...

Two previously undescribed rearranged abietanes, ceratol (1) and ceratodiol (2), and three known abietane-diterpenoids, including 1-ketoaethiopinone (3), ferruginol (4) and 12-deoxysalvipisone (5), were isolated from roots of Salvia ceratophylla. The structures of the purified compounds (1–5) were characterized based on analyses of their spectrosco...

Background and Purpose Oral candidiasis is one of the most common fungal infections in humans. The treatment and prophylaxis of the patients suffering from this infection require the identification of new anti-Candida agents with no side effects or toxicity like medicinal plants. The present study was conducted to compare the antifungal activities...

The chemical compositions of essential oils (EOs) of 11 Salvia species collected from different localities in Iran were analyzed by GC-FID and GC–MS. Altogether, 58 compounds were identified. α- and β-Pinene, 1,8-cineol, β-caryophyllene and sclareol were detected as the major constituents. Moreover, the cytotoxic activity of the EOs were assessed a...

Screening for bioactive compound-producing bacteria is the first step in the drug discovery programs. The aim of the present study was to achieve cytotoxic and antioxidant metabolites producing actinobacteria from the Persian Gulf sediments and to investigate the effect of combinations of seven treatments and six isolation media on the selective is...

Background: The essential oils of Mentha species are used in pharmaceutical, cosmetic and perfume industry and also for flavoring and preservation of several food. Moreover, Mint’s essential oils are also searched for their biological activities such as antimicrobial, antioxidant, and cytotoxic properties. Mentha mozaffarianii is an endemic medicin...

The people of Turkey use Salvia russellii Benth (Lamiaceae/Labiatae) in the Turkish folk medicine to cure common colds, abdominal pains and also it is served as decoction tea, especially in central Anatolia [1]. The plant wildly grows in Turkey, Northern-west and West of Iran, Iraq and Syria [2]. The literature surveys shows that much attentions ha...

Marine microorganisms are important sources for novel natural products. Hence, the aim of this study was to introduce marine microorganisms with the capability of producing antioxidant and cytotoxic metabolites. For this purpose, ten live sea cucumbers (Holothuria leucospilota) were collected from Larak Island, Persian Gulf. Then, their intestine c...

Methanol (MeOH), dichloromethane (DCM) and 80% MeOH extractions of fourteen medicinal plants of the families Solanaceae and Lamiaceae collected from different area of Iran were tested for their cytotoxic potential against MOLT-4 human cancer cell lines. Cytotoxicity of the tested plants indicated that 11 plants had one or two active extracts (IC50≤...

Species of Nicotiana grow naturally in different parts of the world and have long been used both medicinally and recreationally by human societies. More recently in our history, Nicotiana tabacum has attracted interest as one of the most economically important industrial crops. Nicotiana species are frequently investigated for their bioactive natur...

Background The essential oil and three solvent extracts of the aerial parts of Scaligeria nodosa (Boiss.) that were collected in Bamu Mountains (Iran), were screened for their antioxidant, antimicrobial properties and total phenolic contents for the first time. Methods The essential oil was extracted by hydrodistillation and analyzed by capillary...

Background Two cembranoid diterpenes, [(1 S , 2 E , 4 R , 6 R , 7 E , 11 E )-2, 7, 11-cembratriene-4, 6-diol] ( 1 ) and its epimer [(1 S , 2 E , 4 S , 6 R ,7 E ,11 E )-2, 7, 11-cembratriene-4, 6-diol] ( 2 ) were isolated from surface dichloromethane washings and chloroform extract of Nicotiana sylvestris leaves. Methods The compounds were purified...

Salvia lachnocalyx Hedge, Lamiaceae, is an endemic sage which grows naturally in the Fars Province of Iran. The phytochemical analyses of the roots of S. lachnocalyx led to the isolation of five known diterpenoids: ferruginol (1), taxodione (2), sahandinone (3), 4-dehydrosalvilimbinol (4) and labda-7,14-dien-13-ol (5). Their chemical structures wer...

Different solvent extracts of Dichotomaria obtusata (J. Ellis & Solander) Lamark, Galaxauraceae, a red algae collected from the coast of Bushehr in the Persain Gulf, was investigated for its cytotoxic properties and chemical constituents. The fresh alga, after extraction with methanol and dichloromethane were combined and partitioned between water,...

Sahandinone (1), 12-deoxysalvipisone (2), miltirone (3), 7α-acetoxyroyleanone (4), and labda-7,14-dien-13-ol (5) were isolated from the roots of Salvia rhytidea Benth. (Lamiaceae). Their structures were elucidated by a combination of spectroscopic analyses including EIMS and NMR. The 13C NMR spectroscopic data were revised for the quaternary carbon...

1st International Symposium on Phytochemicals in Medicine and Food (ISPMF2015) was hold in Shanghai, China from June 26th to 29th, 2015. 1st ISPMF was organized by Phytochemical Society of Europe (PSE) and Phytochemical Society of Asia (PSA). More than 270 scientists from 48 countries attended this meeting. The programme of ISPMF2015 consisted of 1...

Context The genus Anthemis L. (Asteraceae) comprises about 195 species which are widely used in the pharmaceutical, cosmetic and food industries. Objective Anthemis mirheydari Iranshar, an endemic plant from Iran, was investigated for its cytotoxic properties and chemical constituents. Materials and methods The whole parts of the plant (320 g) were...

Silver is known as an antimicrobial harmless element to the human’s heath for a long time. Nowadays, by emerging multidrug-resistant microorganisms, application of silver in its nano-particle (SNPs) form has raised interests of many researchers in the field of medicinal chemistry. Currently, different strategies of usage and synthesis of SNPs are e...

The volatile constituents of Achillea wilhelmsii, Achillea eriophora and Achillea vermicularis were analyzed by headspace solid-phase microextraction (HS-SPME) and also by hydrodistillation gas chromatography mass spectrometry (HD-GC-MS). Fifty-four and sixty-three compounds were identified in the HS and essential oil (EO) of the plants, among whic...

The dietary flavonoids, especially their glycosides, are the most vital phytochemicals in diets and are of great general interest due to their diverse bioactivity. The natural flavonoids almost all exist as their O-glycoside or C-glycoside forms in plants. The dietary flavonoid C-glycosides have received less attention than their corresponding O-gl...

The plants of the genus Salvia L. are important medicinal herbs of the Lamiaceae family and some of them such as S. officinalis (sage), S. miltiorrhiza (red sage, Danshen) and S. sclarea (clary sage) have been used as medicinal plants in the folk medicine of several countries. In this review, we discuss the reports that have examined Salvia species...

The volatile constituents of Thymus daenensis growing wild in surrounding Shiraz- Iran were extracted by hydrodistillation (HD) and Head-Space Solid-Phase Micro Extraction (HS-SPME) methods and further analyzed by gas chromatography mass spectroscopy (GC-MS). In the HS-SPME and essential oil (EO) of T. daenensis, 40 and 28 components were identifie...

Phytochemical investigation of the flowers of Anthemis odontostephana Boiss. var. odontostephana (Asteraceae) resulted in the isolation of a natural cyclohexenone sesquiterpenoid with a bisabolene skeleton, 1-hydroxydelobanone (1), for the first time as a natural product, and a previously reported cyclohexenone derivative, antheminone A (2), along...

Euphorbia macrostegia or Persian wood spurge is one of the seventeen endemic plants of this genus in Iran. Three triterpenoids, 24-methylenecycloartan-3β-ol (1), butyrospermol (2) and cycloartenol (3) and three diglycerides, 1,2-di-O-α-linolenoyl-sn-glycerol (4), 1-O-linoleoyl-3-O-palmitoyl-sn-glycerol (5) and 1-O-α-linolenoyl-2-O-palmitoyl-sn-glyc...

Different extracts from leaves, epicalyxes and seeds of the plants Hyoscyamus niger and H. reticulatus were subjected to total phenolic measurements using Folin-Ciocalteu reagent and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays and high performance liquid chromatography (HPLC-UV) analyses. Among the tested plant’s extracts, the 80...

The dietary flavonoids, especially their glycosides, are the most vital phytochemicals in diets and are of great general interest due to their diverse bioactivity. The natural flavonoids almost all exist as their O-glycoside or C-glycoside forms in plants. The dietary flavonoid C-glycosides have received less attention than their corresponding O-gl...

The chemical composition of the essential oil of Salvia sharifii growing wild in Iran was investigated by gas chromatography-mass spectrometry (GC-MS). Thirty-nine components representing 84.5 % of the oil were identified. Caryophyllene (12.8 %), germacrene D (9.5 %), trans-isolimonene (7.0 %), spathulenol (6.9%), bicycloegermacrene (5.6 %), caryop...

Context: Finding effective therapies for neurodegenerative diseases is of utmost importance for the aging population. Plants growing in Iran are rich sources of antioxidants and active phytochemicals. Objective: The protective capacity of plants, with a special focus on those with reported antioxidant or neuroprotective potential or nervous syst...

Plagiodera versicolora, a willow beetle living on S. sachalinensis, is found on S. integra during early June in Hokkaido Island, Japan. This insect selects several species of willows (Salix), including S. integra as host plant in Honshu Island of Japan. To determine the reasons for the limited distribution of this beetle on the willows of Hokkaido,...

Antibacterial bioassay guided fractionation of acetone extracts of Astragalus brachystachys resulted in isolation of sclareol and two related labdane-type diterpenoids, 14R-epoxysclareol and 6beta-hydroxysclareol. The antibacterial activity of the isolated compounds was measured and it was deduced that the epoxidation at the double bond of sclareol...

To investigate the role of secondary metabolites in the feeding behavior of Manduca sexta larvae feeding on Nicotiana attenuata, an aqueous acetone extract of the aerial parts of the plant was subjected to feeding-performance bioassay-guided fractionation. We isolated three 20- hydroxygeranyllinalool glycosides from the leaves of N. attenuata, whic...

In boiling pyridine, lapachol (1) is transformed into novel asymmetric naphthoquinone derivative, quadrilone (2), and into dehydro-α-lapachone (3), and adenophyllone (4). Lapachol used in the present study was isolated from the heartwood of Heterophragma quadriloculare and found to be the most abundant naphthoquinone.

Acetone extract of aerial parts of Euphorbia marschalliana collected from Iran has been subjected to different chromatography techniques for fractionation and purification. The stereo-structures of the myrsinol esters 15-O-acetyl-3-O-propionyl-5-O-butanoyl-7-O-nicotinoylmyrsinol (1) and 15-O-acetyl-3,5-O-dibutanoyl-7-O-nicotinoylmyrsinol (2) have b...

The essential oil of Anisosciadium orientale was analyzed by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The essential oil (EO) was rich in monoterpene hydrocarbons: terpinolene (19.7±2.8%, 226.4±27.6 mg/ g EO), limonene (21.0±1.3%, 313.3±22.9 mg/ g EO) and myrcene (5.6±0.50 %, 65.4±7.2 mg...

Euphorbia microsciadia (Euphorbiaceae) is a plant growing wild in different parts of Iran. The roots of E. microsciadia after grinding were extracted with dichloromethane, acetone, methanol and methanol-water (80:20) separately. These four extracts were screened for their antioxidant and antimicrobial properties. Total phenolic content of the extra...