Amir Afshin Khaki

University of Lübeck · Gynecology, Embryology & infertility

The present study aimed to evaluate the impact of N-acetylcysteine (NAC) on testicular hypoxia caused by varicocele, focusing specifically on the regulation of genes related to apoptosis and oxidative stress in the testes of mature Wistar rats. Thirty-two rats were divided into four groups: Control (Sham), hypoxia, testicular hypoxia treated with N...

Testicular tissue hypoxia is a state in which tissues do not receive enough oxygen to meet their demand. One disease known to cause this hypoxic condition is varicocele (VCL), which can negatively impact the testicles and lead to poor sperm quality. Previous research has indicated that antioxidants play a significant role in protecting against the...

Background. Diabetes mellitus is a condition that causes biochemical and physiological changes in the body. In this study, the effects of carvacrol on blood biochemical factors related to diabetes in diabetic adult rats induced by streptozocin were investigated. Methods. In this study, 32 male Wistar rats were divided into four groups as follows: a...

About a third of human infertility is related to male factors. Of these, idiopathic-related infertility is not curable. Diabetes mellitus is a metabolic disorder affecting male impotence and fertility by increased production of free radicals and oxidative stress. Saponin, a glycosidic compound found in many plants, improves sperm parameters. The pr...

Objectives: This study aimed to investigate the role of Eugenol in regulating the expression of FSH receptor (FSHR), human luteinizing hormone choriogonadotropin receptor (LHCGR), and estrogen receptor (ER) during follicular development in female rat ovarian torsion. Materials and Methods: In this experimental study, 48 female rats were randomly as...

In the present work, we examined the beneficial efficacy of chrysin as a natural flavonoid on testicular torsion/detorsion damages among adult male rats. Forty-eight male Wistar rats were randomly allocated into six groups: sham, torsion/detorsion (TD) group, TDC30 group in which TD process was applied and treated with chrysin (30 mg/kg), TDC50 gro...

Objectives: This study aimed to evaluate the effect of crocin and treadmill exercise on oxidative stress, sperm parameters, and testis structure in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: In this experimental study, 64 diabetic rats induced by a single intraperitoneal (IP) injection of STZ (50 mg/ kg) were assigned to the...

Objectives: This study aimed to evaluate the effect of anthocyanin on oxidative stress, sperm, and testis structure in diabetic rats induced by streptozotocin (STZ). Materials and Methods: In this experimental research, diabetes was induced by a single intraperitoneal injection of STZ (50 mg/ kg). A total of 64 rats were assigned into four groups a...

The current research aimed to assess the impacts of Minocycline on varicocele-induced regulation of apoptotic-related genes and oxidative stress in the testis of adult Wistar rats. Thirty-two rats were divided into the 4 groups: sham, varicocele (VcI), varicocele treated with Minocycline (VcI+Mno) for 56 days, and healthy rats treated with minocycl...

The current study was designed to investigate the effect of eugenol on histopathological changes and oxidative stress caused by torsion /detorsion in the ovary of adult female rats. In this study, forty-eight female Wistar rats were classified into six groups; Sham and 2 healthy group receiving 30, 60 mg/kg eugenol; ovarian torsion/detorsion; and 2...

Diabetes mellitus (DM) is a complex metabolic disease and it is also closely associated with a reduction in fertility in male patients. The purpose of the present study was to investigate the antidiabetic effect of carvacrol (CRV), as a potent antioxidant, on the numbers of germ cells and Sertoli cells in testicular tissue, and the messenger RNA (m...

Introduction: To evaluate the effect of a herbal mixture (i.e., Mentha spicata, Zingiber officinale, Cinnamomum zeylanicum, and Citrus sinensis) alone and in combination with clomiphene citrate (CC) compared to CC on the treatment of polycystic ovary syndrome (PCOS). Material and methods: This single-blind randomized clinical trial was conducted...

Objective: Panax ginseng is a popular traditional herb that has been used for complementary and alternative medicine in eastern Asia, because of its pharmacological compounds, ginsenosides. This study aimed to investigate the impact of Panax ginseng extract (PGE) at different concentrations on in vitro follicular function and development in the thr...

Background: Varicocoele is a swollen bulge of the pampiniform venous plexus inside the scrotum. It is also considered one of the causes of infertility in males. It has been demonstrated that hesperidin has remarkable pharmacological potentials, including antioxidant, anti-inflammatory, antimicrobial, and anticarcinogenic effects. Objective: The pre...

Objectives: In this experimental research, a rat model was used to investigate the protective effect of Fumaria parviflora on oxidative stress and testis tissue damage in diabetic rats. Materials and Methods: To this end, a total of 28 male Wistar rats were utilized and randomly divided into 4 groups including control group (G1), diabetic control g...

Objectives:In this experimental research, a rat model was used to investigate the protective effect of Fumaria parviflora on oxidative stress and testis tissue damage in diabetic rats.Materials and Methods: To this end, a total of 28 male Wistar rats were utilized and randomly divided into 4 groups including control group (G1), diabetic control gro...

Introduction: This study investigated the effects of extremely low frequency electromagnetic fields (ELF-EMF) (50 Hz, 3 mT) on biochemical parameters of rats’ ovarian tissues and the impact of Allium cepa on the reduction of potential adverse influences of electromagnetic exposure. Material and Methods: In this study 40 female Wistar rats were divi...

Background: Diabetes is one of the most chronic and widespread diseases causing the damages to the male reproductive system. Nowadays, several studies have been performed to show the role of phenolic compounds in reducing the complications of diabetes. Carvacrol is a phenolic monoterpene which has been shown to have much therapeutic efficacy in var...

Objectives: The aim of the present research was to Compression the Role of hydroalchoholic extract of Apium Graveolens and Cinnamon Zeylanicum on metabolically change and ovarian oxidative injury in a rat model of polycystic ovary syndrome. Materials and Methods: In our research, experimentally, 64 female rats with Wistar Breed were used (n=8/grou...

Objectives:"Polycystic ovary syndrome" (PCOS) known as one of the most usual hormone disturbance in women of childbearing age, according to it, the aim of this research was to investigate the Effect of hydroalchoholic extract of Cinnamon Zeylanicumon oxidative damages and biochemical change in adult rat with PCOS Materials and Methods: In our expe...

Objectives: In this experimental study, a testicular torsion/detorsion model was used in male rats to investigate the effect of onion juice on male fertility factors and rate of pregnancy by intrauterine insemination (IUI). Materials and Methods: A total of 56 Wistar rats (28 males vs. 28 females) were included in this study. Male rats were randoml...

Introduction Infertility is one of the main problems for the couples. According to statistical prevalence, approximately 15% of the couples are encountered with infertility at the end of the first year of marriage and about 20% of these cases are occurred in males (1). Furthermore, in recent decades, the number of couples searching for consultation...

Background: The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomilla (MC) on preantral follicle culture of mouse ovaries in a three‑dimensional culture system. Methods: Isolated preantral follicles were randomly divided into three main groups: the control group containing 10% fetal bovine serum w...

The aim of this study was to evaluate the effects of Permethrin on the testes of adult Wistar rats, and evaluate the efficacy of Onion juice supplementation. The male rats were divided into five groups: I: control; II: DMSO (0/5 ml); III: Permethrin (35 mg/kg) in 0/5 ml DMSO; IV: Permethrin (35 mg/kg) + Onion (3 ml); V: onion (3 ml). After 60 days,...

Environmental factors, such as electromagnetic waves, induce biological and genetic effects. One of the most important physiological systems involved with electromagnetic fields (EMFs) is the genital system. This paper reviews the effects of EMFs on human reproductive organs, female animals, fetus development and the importance of two types of natu...

Objectives: In this study we investigated whether 50 Hz power frequency electric field of 1.01×10³ V/m and the magnetic field flux density of 3 mT have effect on serum sex hormones and antioxidant capacity and if Allium cepa and cinnamon can moderate potential adverse effects of power frequency electromagnetic exposure. Materials and Methods: Forty...

Introduction: In recent years, there has been an increase in the attention paid to safety effects, environmental and society's health, extremely low frequency electromagnetic fields (ELF-EMF), and radio frequency electromagnetic fields (RF-EMF). The aim of this research was to determine the effect of EMF on the alteration of ovarian follicles. Me...

Background: Recently, there are increasing concerns and interests about the potential effects of Electromagnetic Field (EMF) on both human and animal health. Objective: The goal of this study was to evaluate the harmful effects of 50 Hz non-ionizing EMF on rat oocytes. Materials and methods: In this experimental study 30 rats were randomly tak...

Background: Diabetes rats have been linked to reproductive dysfunction and plant medicine has been shown to be effective in its treatment. Antioxidants have distinctive effects on spermatogenesis, sperm biology and oxidative stress, and changes in anti-oxidant capacity are considered to be involved in the pathogenesis of chronic diabetes mellitus....

The effect of electromagnetic field (EMF) as an environmental factor on different organs including female reproductive system is of critical concern. The aim of the present study is to evaluate the effect of low-frequency (LF)-EMF on oocyte differentiation and follicular development. The experiment was carried out in animal lab of Faculty of Medici...

Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans. It can also be done by preservation of ovarian tissue or entire ovary for transplantation, followed by oocyte harvesting or...

We performed this study to evaluate use of fresh and frozen sperm samples in non-obstructive azoospermia microdissection testicular sperm extraction (micro-TESE-ICSI) treatment. We performed a total of 82 consecutive in vitro fertilization (IVF) cycles at Fertijin IVF Center in Istanbul, Turkey from January 2010 to March 2012. In 43 participants we...

Single embryo transfer is becoming increasingly popular in IVF/ICSI. More IVF/ICSI cycles therefore include freezing of high quality embryos, and the cumulative effect of such cycles becomes more important. To improve the results obtained using frozen–thawed embryos, the predictive value of embryo and patient characteristics such as ovarian reserve...

Objective(s):Cinnamon as an antioxidant is able to reduce oxidative stress. Previous study confirmed antioxidant enzymes have beneficial and essential effects on male infertility by their role against reactive oxygen species.Material and Methods: Wistar male rats (n=20) were allocated into two groups, control group (n=10) and cinnamon group (n=10)...

Background: Cyclo-oxygenase-2 (COX-2) specific inhibitors were examined for predication or treatment of different tumors and it is indicated that COX-2 specific inhibitors play an important regulatory role in apoptosis of tumoral tissues. Therefore, the present study was designed in order to examine the preventive effects of a COX-2 specific inhibi...

Cinnamon is an antioxidant and it has been shown to reduce oxidative stress. Previous study confirmed antioxidants enzymes have imbalanced on diabetes by hyperglycemia role on reactive oxygen's spaces and is one of the male infertility agents. Wistar male rat (n=40) were allocated into four groups, control group (n=10), Cinnamon group that received...

Medicinal use of basil, Ocimum basilicum, dates back to ancient times in Iran, China, and India. This herb has been used since ancient times as a medicine and food and it is known that the antioxidant effect of O. basilicum is beneficial to protect tissue and decreasing carcinogenic effect of EMF, so it was hypothesized that this herb might also pr...

Male reproductive health has been under scrutiny recently. Many studies in the literature have concluded that semen quality is declining and that the incidence of testicular cancers is increasing. The reason for this change has been attributed to damage in sperm chromatin. During in vivo reproduction, the natural selection process ensures that only...

Cryopreservation of human oocytes and embryos or blastocyts is an important choice in assisted reproduction treatment that leads to an increased cumulative outcome while decreasing other attempts’ costs. It provides an opportunity for patients to have more than one attempt following an ovarian stimulation cycle, thereby decreasing the exposure of p...

To study the role of Quercetin in streptozotocin-induced diabetes in rats. Wistar male rat (n=40) were allocated into three groups, control group (n=10) and Quercetin (QR) group received 15 mg/kg (IP) QR, (n=10), and diabetic group that received 55 mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10; STZ group and treatm...

Social changes, uprising ambitions and life demands have markedly affected the behavior and attitude of women toward the concept of composing a stable family and giving birth to children, that was clearly demarcated by the remarkably increased age of conception and child birth, postponement of such essential events could be followed later on by a s...

Medicinal use of basil, Ocimum basilicum, dates back to ancient times in Iran, China and India. In this study, we aimed to experiment on the antioxidative property of a herbal source (sweet basil) to protect central nervous system against oxidative damages of electromagnetic field (EMF) and it's affective sequences. We used forced swimming test to...

Medicinal use of basil, Ocimum basilicum, dates back to ancient times in Iran, China, and India. This herb has been used since ancient times as a medicine and food and it is known that the antioxidant effect of O. basilicum is beneficial to spermatogenesis, so it was hypothesized that this herb might also provide protection to sperm parameters. Mal...

Background: The project is designed to evaluate the effect of intrauterine exposure to 50Hz electromagnetic field (EMF) on testicular development. Method: Pregnant BALB/c mice were exposed to 3 μT EMF for 21 days, 4 hours/day. Unexposed pregnant mice were used as controls. After delivery, testes were removed from male pups, fixed and prepared for l...

Toxoplasma gondii is a protozoan parasite that is globally widespread and infects man and animals; we investigated effect of Allium cepa on sperm parameters, testestrone level in male rats experimentally infected by toxoplasma. Wistar male rats (n=40) were allocated into four groups, control group (n=10) and T 1 group that received tachyzoites of T...

Citrus fruits have long been recognized as containing valuable sources of important nutrients which are biologically active in humans. Citrus fruits, such as oranges, contain compounds called phytochemicals that can be included into three major groups: the flavonoids, limonoids and carotenoids. The flavonoids are a group of benzopyran derivatives w...

Axillary artery is one of the most important arteries of the upper limb, which is a continua- tion of the subclavian artery. It begins at the lateral border of the first rib and ends at the inferior border of the teres major where it becomes the brachial artery. Axillary artery has six important branches included: 1) Superior thoracic artery 2) Tho...

Background: In the industrial world, almost everyone is unavoidably exposed to ambient electromagnetic field (EMF) generated from various technical and household appliances. Objective: Controversy exists about the effects of EMF on various tissues of the living bodies. Uterus play an important role in reproductive and the effects of EMF on its tiss...

Medicinal use of basil, Ocimum basilicum, dates to ancient times in Iran, China and India. This herb has been used since ancient times as a medicine and food and it is known that the antioxidant effect of O. basilicum is beneficial to spermatogenesis, so it was hypothesized that this herb might also provide protection to sperm parameters. Male Wist...

Background: Alcoholic mothers who consume ethanol give birth to infants with high percentage of hepatic diseases. Alcohol can cause cellular damage in different tissues, including liver. Finding a drug which is effective and efficient in reducing ethanol misuse consequences during pregnancy can assist the decrease of harmful effects of this habit....

Lead acetate is an example of heavy metals that for decades being known for its adverse effects on various body organs and systems such that their functions are compromised. In the present study, the ability of lead to adversely affect the liver tissue apoptosis was investigated. Ginger is source of antioxidants was administered orally to prevent t...

Background: Plants are rich in phenolics (phenolic acids, flavonoids and Tannins). The interest in phenolics has been increased by recent reports of their antioxidant activities. These antioxidant nutrients from food sources reduce the harmful effects of oxidative stress and offer health advantages. Objective: Total phenolics, flavonoids contents a...

Coronary angiography is one of the most common hospital procedures, though there is limited information on the location and extent of coronary artery blockages other than for predominately White patients. This study examined the location and magnitude of coronary artery blockages in patients admitted to a leading tertiary heart hospital in Iran wit...

Background: Ginger rhizome (Zingiber officinale R., family: Zingiberaceae) is used medicinally and as a culinary spice. Objective: Medicinal use of ginger dates back to ancient China and India. Ginger and its constituents are stated to have antiemetic, antithrombotic, antihepatotoxic, anti-inflammatory, stimulant, cholagogue and antioxidant. It has...

To compare the preventive effect of dietary and topical Celecoxib (CCB), a potent inhibitor of COX-2 on 4-NQO-induced tongue SCC in rat. Fifty male Sprague Dawley adult 3- 3.5 months old rats were used as animal model in this study. The tongue SCC was induced by a daily administration of 30 ppm 4-NQO, in drinking water, for 8 months. The rats in ca...

The aim of the study is to determine the effect of Celecoxib administration, dietary or topical, on expression of Ki-67, c-erb-B2, bcl-2 and bax genes in rat tongue by the immunohistochemistry methods and also tdt-mediated dupt-biotin nick end labeling assay in order to explore their role in malignant transformation and the proliferation rate, apop...

The American Journal of Gastroenterology is published by Nature Publishing Group (NPG) on behalf of the American College of Gastroenterology (ACG). Ranked the #1 clinical journal covering gastroenterology and hepatology*, The American Journal of Gastroenterology (AJG) provides practical and professional support for clinicians dealing with the gastr...

Allium cepa (onion) has a beneficial effect on disease treatment worldwide and has been used since ancient times as a medicinal and food source. Recently several reports have shown that onion has high antioxidant activity. As antioxidants have an essential effect on sperm health parameters, we investigated the effect of the fresh juice of onion bul...

Background: Quercetin is a strong antioxidant and long-term treatment of STZ-diabetic animals and it has been shown to reduce oxidative stress. Objective: Antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathoge...

Enalapril Maleate is an antihypertensive drug that reduces blood pressure by dilatation of blood vessels as well as diminishing systolic and diastoles blood pressure. We wanted to assess its histopathology effects on fetal heart in rat. In this survey twenty male and female Wistar rats were selected and matched together, after getting pregnant, the...

The aim of this study was to investigate the comparative effects of aminoglycosides and fluoroquinolones on testis apoptosis and sperm parameters in rats. Fifty male Wistar rats were randomly divided into control (n = 10) and experimental (n = 40) groups. The experimental groups subdivided into four groups often. Each received 5 mg kg(-1) (IP) gent...

The present study examined the histopathologic effects of this gas on the mouse liver in acute and chronic exposure. Twenty eight male mice were divided into 4 groups. Group 1 (G1) as control ( not exposed to gas ), group 2 (G2) were exposed to high dose of (100 ppm) and group 3 (G3) and group 4 (G4) were exposed to low dose of sulfur dioxide for 5...

In the industrial world, almost everyone is unavoidably exposed to ambient electromagnetic field (EMF) generated from various technical and household appliances. Controversy exists about the effects of EMF on various tissues of the living bodies. Seminal vesicles as one of these accessory glands play an important role in natural seminal fluid forma...

Background: Men are unavoidably exposed to ambient electromagnetic fields (EMF)generated from various electrical gadgets and from power transmission lines. Prostategland plays an important role in secretion of semen as largest accessory gland of malereproductive system. It seems that protection of this gland against EMF is important inspermatogenes...

Background: Ciprofloxacin is a commonly prescribed antibiotic in the treatment of genitourinary tract infection. Objective: The aim of this study was to investigate the effects of ciprofloxacin on testis apoptosis and sperm parameters in rat. Materials and Methods: Twenty male Wistar rats were selected and randomly divided into two groups; control...

Fetus-in-fetu is a rare congenital condition in which a malformed fetus-like structure is found in the body of its twin. We report a unique case of a male neonate with cleft palate and a fetus-like structure arising in his oral cavity. The neonate underwent emergent surgical removal of the mass immediately after delivery. Radiological and pathologi...

Introduction & Objectives: Methotrexate (MTX) is a chemotherapeutic agent that used for the treatment of a variety of tumors and inflammatory diseases. The aim of this study was planed to see the role of human growth hormone (hGH) on testis recovery after treatment with MTX in rat. Materials & Methods: In this experimental study the fifty male wis...

Wandering spleens are rare clinical entities found more commonly in females. We report a young female patient found to harbour a pelvic spleen. The literature regarding this rare ectopia is reviewed. The wandering spleen should be considered in the differential diagnosis of pelvic masses.

Human beings are unavoidably exposed to ambient electro magnetic fields (EMF) generated from various electrical gadgets and from power transmission lines. Controversy exists about the EMF effects on various organs. One of the critical issues is that EMF may adversely affect the reproductive system. To examine this, rat pups (30) were exposed to 50...

Variation in the course of the left recurrent laryngeal nerve is seemingly very rare. During the routine dissection of an adult male cadaver, the entire left recurrent laryngeal nerve after branching from the left vagus nerve was noted to travel medial to the ligamentum arteriosum. We hypothesize that this rare variation may occur, if the left recu...