Amina Kurtovic-Kozaric

Amina Kurtovic-Kozaric
International BURCH University · Department of Genetics and Bioengineering

PhD

About

70
Publications
16,184
Reads
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1,508
Citations
Additional affiliations
June 2014 - present
University of Sarajevo
Position
  • Professor (Associate)
September 2012 - July 2013
Cleveland Clinic
Position
  • Fulbright Visiting Scholar
June 2007 - present
University of Sarajevo
Position
  • Professor (Associate)

Publications

Publications (70)
Article
Full-text available
Background Clinical trials (CTs) are research investigations in which participants receive medical treatments, interventions, or tests to assess their safety and efficacy. Each planned clinical is registered through local or national medical agencies, which may differ in the amount of administrative and legal procedures. The objective of this study...
Article
p>Here we describe the major genetic and genomic aberrations found in myeloid malignancies and how those markers are used in patients’ diagnosis, prognosis, and targeted treatment. In Bosnia and Herzegovina, cytogenetic and molecular diagnostics for myeloid malignancies have been established and continually improved since 2005. We report the curren...
Article
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The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing , a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands of samples in parallel. SARSeq relies on next generation sequencing of multiple amplicons g...
Preprint
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Bosnia and Herzegovina is among ten countries in the world with the highest mortality rate due to COVID-19 infection. Lack of lockdown, open borders, high mortality rate, no herd immunity, no vaccination plan, and strong domestic anti-vaccination movement present serious COVID-19 concerns in Bosnia and Herzegovina. In such circumstances, we set out...
Article
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In developing countries, TKI is limited and many patients have delayed the start of therapy. The superiority of nilotinib in delayed treatment is not well studied. We have previously recognized the possible superior effect of delayed nilotinib and decided to analyse long-term effects. In this study we presented long-term outcomes of 70 CML patients...
Preprint
Full-text available
During a pandemic, mitigation as well as protection of system-critical or vulnerable institutions requires massively parallel, yet cost-effective testing to monitor the spread of agents such as the current SARS-CoV2 virus. Here we present SARSeq, saliva analysis by RNA sequencing , as an approach to monitor presence of SARS-CoV2 and other respirato...
Article
Full-text available
Background: Metanephric adenoma (MA) is a rare benign renal neoplasm. On occasion, MA can be difficult to differentiate from renal malignancies such as papillary renal cell carcinoma in adults and Wilms̕ tumor in children. Despite recent advancements in tumor genomics, there is limited data available regarding the genetic alterations characteristic...
Article
Full-text available
Aim To investigate quality of life and exposure to lifestyle risk factors of cancer patients in Bosnia and Herzegovina and a correlation of cancer type with lifestyle risk factors. Methods This was a cross-sectional study conducted on 200 cancer patients from the Clinical Centre of the University of Sarajevo. The respondents completed an anonymous...
Article
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Cell-to-cell communication is a fundamental process in every multicellular organism. In addition to membrane-bound and released factors, the sharing of cytosolic components represents a new, poorly explored signaling route. An extraordinary example of this communication channel is the direct transport of mitochondria between cells. In this review,...
Conference Paper
Background: Chronic myeloid leukaemia (CML) is a clonal disease of the haematopoietic stem cells that is driven by the chimeric BCR-ABL1 protein with aberrant tyrosine kinase activity. Chimeric BCR-ABL1 p210 protein results from several types of transcripts, where e13a2 and e14a2 account for the vast majority of cases. Aims: We analysed the correla...
Article
Acute erythroleukemia (AML-M6 or AEL) is a rare but aggressive hematologic malignancy. Previous studies showed that AEL leukemic cells often carry complex karyotypes and mutations in known AML-associated oncogenes. To better define the underlying molecular mechanisms driving the erythroid phenotype, we studied a series of 33 AEL samples representin...
Article
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The identification of mutually exclusive somatic mutations shared among myeloproliferative neoplasm (MPN) subtypes has provided a powerful tool for studying disease evolution. Clinical features, gene mutations, and survival over 18 years were analyzed in MPN patients. One hundred thirty-eight MPN patients were subcategorized according to MPN subtyp...
Article
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The odontogenic keratocyst (OKC) may occur at any age. However, it mostly occurs during the second and third decades of life. Compared to other odontogenic cysts, this type occurs with a frequency of 5-15%. It is more common in the mandible region and in the male sex. Histologically, odontogenic keratocysts are characterized by the presence of an e...
Article
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Objectives: In silico bioinformatical analysis suggested that the expression of two genes, CCL5 (C-C Motif Chemokine Receptor 5) and ep300 (Histone acetyltransferase p300), could be used as potential new biomarkers in differentiation between periapical granulomas and radicular cysts. Thus, we hypothesized that gene expression of CCL5 and ep300 in...
Poster
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The poster describes the variants of the MTHFR gene and their association with clinical conditions.
Conference Paper
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Introduction: Myeloproliferative neoplasms are a group of clonal myeloid disorders. The aim of this study was to evaluate disease phenotypes and evolution in JAK2, CALR and MPL mutations in the population of MPN patients in Bosnia and Herzegovina. Materials and methods: We included 138 MPN patients (PV (n=41), ET (n=56), PMF (n=10) and MPN-U (n=31)...
Conference Paper
Full-text available
Introduction: TKI therapy for CML patients, including imatinib and nilotinib, was gradually introduced in Bosnia and Herzegovina because of financial considerations and lack of insurance cover, which had drastic consequences on patients' outcomes. Aim of this study was to compare the long-term clinical outcomes of CML patients receiving delayed and...
Conference Paper
Full-text available
Introduction: Treatment of patients with myeloid malignancies is suboptimal in Bosnia and Herzegovina due to the lack of targeted therapies. The aim of this study was to collect all available data in the last 20 years. Methods: Patients with myeloid neoplasms (n=271) treated at the Clinical Center of the University of Sarajevo, B&H, were included i...
Article
Background Philadelphia-negative myeloproliferative neoplasms (MPNs), including PV, ET, PMF, and MPN-U are a group of clonal myeloid disorders characterized by myeloproliferation without dysplasia. WHO 2016 revision lists the presence of driver mutations as one of several major criteria in the diagnosis of MPN patients. MPN subtypes have overlappin...
Chapter
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Odontogenic cysts are a group of common pathological lesions of the jaw. Typically, they can be found randomly on X-rays as round benign lesions. However, some of them can behave aggressively with a tendency toward malig-nancy. Among odontogenic cysts with benign pathology, up to 60% of all jaw cysts are radicular cysts, which originate from root c...
Article
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BACKGROUND: AFP serum levels are considered as diagnostic and specific for hepatocellular carcinoma (HCC) in patients with liver cirrhosis (LC). AIM: This study aimed to examine the diagnostic value of AFP in the distinguishing of patients with HCC from patients with LC, and to analyse the potential correlation between AFP levels and liver disease...
Article
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Purpose: In this study, we analyzed biochemical parameters in the serum of patients with a diagnosis of hepatocellular carcinoma (HCC) C and B viral aetiology. Material/Methods: All patients (31 males and 20 females) with a diagnosis of HCC that were treated at the Clinical Centre of the University of Sarajevo were included in this retrospective-pr...
Article
Background/Aims The best treatment for an avulsed tooth is immediate replantation. If this is not possible, a proper transport medium is required for the maintenance of viability of the periodontal ligament cells (PDL).The aim was to systematically review the efficacy of different storage media used for the survival of PDL cells of avulsed teeth in...
Article
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Cancer is referred to as malignancy and presented uncontrolled proliferation of cell. Although some of the types are mostly observed, some of them could be in rare amount. Cancer can be affected by many factors including smoking, drinking, obesity, hormones, and environment. Biostatistics is a powerful tool for describing and summarizing different...
Article
Background Philadelphia-negative classical myeloproliferative neoplasms (MPNs) are a heterogenous group of disorders characterized by cellular proliferation of one or more hematopoietic cell lines. In 2016, after revision of the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, the detection of somatic mutations in three driver...
Article
Since the first widespread use of depleted uranium in military in the 1991 Gulf War, the so-called "Gulf War Syndrome" has been a topic of ongoing debate. However, a low number of reliable scientific papers demonstrating the extent of possible contamination as well as its connection to the health status of residents and deployed veterans has been p...
Article
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Background The usage of Artificial Neural Networks (ANNs) for genome-enabled classifications and establishing genome-phenotype correlations have been investigated more extensively over the past few years. The reason for this is that ANNs are good approximates of complex functions, so classification can be performed without the need for explicitly d...
Poster
Background: Gastrointestinal stromal tumors (GIST) are common mesenchymal neoplasms arising within the gastrointestinal tract from precursors of the connective-tissue cells. The objective of the study is to analyze immunohistochemical and histopathological features of GIST with a focus on CD117 expression as a relevant prognostic factor for ten-yea...
Poster
Introduction: Genetic factors related to male infertility are chromosomal abnormalities, gene mutations, and microdeletions on Y chromosome, which are detected in about 15% of all cases of male infertility. The aim of this study was to identify the site specific microdeletions of Y chromosome in idiopathic azoospermic and oligozoospermic cases from...
Article
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e18549 Background: In this study we examined the long-term real life clinical outcomes of patients receiving front- line nilotinib and patients who switched from branded or generic imatinib to nilotinib (second-line therapy) in Bosnia and Herzegovina. Methods: Patients in CML-CP (n = 70) who started their TKI treatment in period from June 2005 to A...
Article
1537 Background: Renal metanephric adenoma (MA) is a very rare benign renal tumor, which is frequently misclassified when microscopic features alone are applied. Despite the classification of adenoma as a benign tumor, it is difficult to differentiate from other renal carcinomas such as malignant papillary renal cell carcinomas and in children it c...
Article
Full-text available
Patients with cancer in developing and low-income countries have limited access to targeted cancer therapies. The transitional nature of these economies has influenced health care funding, which has resulted in the unavailability of targeted cancer treatments.1,2 Besides the three studies that will be described here, to our knowledge, no literature...
Article
The main aim of this review is to summarize and discuss the current state of knowledge on chemical toxicity and radioactivity of depleted uranium (DU) and their effect on living systems and cell lines. This was done by presenting a summary of previous investigations conducted on different mammalian body systems and cell cultures in terms of potenti...
Article
In recent years, contradicting data has been published on the connection between the presence of depleted uranium and an increased cancer incidence among military personnel deployed in the Balkans during the 1992-1999 wars. This has led to numerous research articles investigating possible depleted uranium contamination of the afflicted regions of t...
Article
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Since early diagnosis plays an important role in oral cancer prognosis, a general dental practitioner’s ability to recognize the clinical signs and symptoms is crucial. This review describes the role of general dental practitioners in the recognition of the early stage of oral cancer. To create guidelines for general dental practitioners, several a...
Article
Aberrant methylation is one of the driving forces of cancer genome development. Although the rate of methylation appears massively variable across the genome, it is mainly observed in histone modification, chromatin organization, DNA accessibility, or promoter sequence. Methylation of promoter sequence occurs mostly to cytosine nucleotides, which c...
Article
Renal metanephric adenoma (MA) is a rare benign tumor frequently misclassified when microscopic features alone are applied. The correct classification of a renal tumor is critical for diagnostic, prognostic, and therapeutic purposes. Despite the advancements in cancer genomics, up until recently relatively few genetic alterations critical to MA dev...
Article
Imatinib mesylate, a selective BCR-ABL tyrosine kinase inhibitor, has been well established as the standard of care for chronic myeloid leukaemia patients. In this study, we compared clinical outcomes of patients who received first-line Glivec (Group 1) with patients who received first-line generic imatinib (Group 2) in Bosnia and Herzegovina with...
Article
Introduction: Generics of imatinib mesylate, the first tyrosine kinase inhibitor targeting the BCR-ABL1 fusion protein, have recently been approved in many countries as the alternative, low-cost forms for the treatment of patients with chronic myeloid leukemia (CML). The aim of this study was to evaluate the long-term clinical outcomes of patients...
Article
Full-text available
Background: Heteromorphic variants including Yq12 material, being inserted or added to autosomal chromosomes have been reported for chromosomes 1, 7, 11, 13, 14, 15, 21 and 22. Here we describe a novel insertion of Yq12 heterochromatin into a chromosome 17; to the best of our knowledge no similar cases have been reported previously. Methods: GTG-,...
Article
Introduction Imatinib mesylate (Glivec, Novartis) is the first tyrosine kinase inhibitor (TKI) targeting the BCR-ABL1 fusion protein responsible for the pathogenesis of chronic myeloid leukemia (CML). Low cost generic alternatives to imatinib are an integral part of cost effective healthcare strategies for developing countries. However, the use of...
Article
This study aims to compare the advantages of two widely used methods for fetal chromosomal detection, karyotyping and QF-PCR, together with the indications for invasive prenatal diagnosis. We retrospectively investigated 888 amniocenteses analyzed by karyotyping only or karyotyping combined with QF-PCR. We assessed the results of each method and co...
Article
Full-text available
Background: Cancer patients in developing and low income countries have limited access to target therapies. Many patients with gastrointestinal stromal tumors (GIST) have had to wait for extended period of time to receive imatinib mesylate (0-67 months), the standard treatment for patients with advanced/metastatic disease. Some patients never recei...
Article
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Mixed-lineage leukemia (MLL) is a subtype of acute myeloid leukemia with more aggressive prognosis than other subtypes. Translocations of MLL gene with other partner genes, forming the MLL-fusion proteins (MLL-FPs), are the main characteristics of MLL leukemia. Many studies have demonstrated that MLL-FPs such as: MLL-AF4, MLL-AF6, MLL-AF9, MLL-AF10...
Article
Full-text available
Microdeletion syndromes are caused by chromosomal deletions of less than 5 megabases which can be detected by fluorescence in situ hybridization (FISH). We evaluated the most commonly detected microdeletions for the period from June 01, 2008 to June 01, 2015 in the Federation of Bosnia and Herzegovina, including DiGeorge, Prader-Willi/Angelman, Wol...
Article
Full-text available
Invasive breast cancer is a heterogeneous disease. The analysis of one or a group of specific gene expression profiles may not be enough to understand molecular activities in cancer cells. Therefore, a method which gives us the opportunity to compare similar up and down regulated gene expression profiles, is needed. The main purpose of our work is...
Article
Full-text available
Gaucher disease is a hereditary genetic abnormality which defects the pathway of sphingolipid catabolism. The mutation of GBA gene which encodes lysosomal β-glucosidase enzyme is the main characteristics of the disease also is observed in different cancer types. To find the relation between the disease and colon adenocarcinoma, the responsible gene...
Article
Full-text available
Cancer patients in developing and low-income countries have limited access to target therapies. For example, tyrosine kinase inhibitor (TKI) therapy for chronic myeloid leukaemia patients (CML) is often delayed. In Bosnia, 16% of patients received immediate TKI treatment (<3 months of diagnosis), while 66% of patients received therapy after a media...
Conference Paper
Background: MLL leukemias (mixed lineage leukemia) have unique clinical and biological characteristics. MLL partners with over 50 different genes that result in the expression of chimeric proteins.The chromosomal translocations t(9;11), t(11;19) and t(4;11) leading respectively to MLL-AF9, MLL-ENL and MLL-AF4 fusions are the most frequent. Methods:...
Article
Full-text available
The guanine rich locations are present in human genome. Previous studies have shown that the presence of G rich sequences and motifs may be significant for gene activity and function. We decided to focus our interest to identify G rich motifs in promoters of oncogenes and tumor suppressor genes. We used a set of 100 most common oncogenes and tumor...
Article
Full-text available
Background Periapical inflammatory lesions have been investigated previously, but understanding of pathogenesis of these lesions (granulomas and radicular cysts) at the molecular level is still questionable. Matrix metalloproteinases (MMPs) are enzymes involved in the development of periapical pathology, specifically inflammation and tissue destruc...
Article
Full-text available
microRNA is a noncoding RNA sequence, 20-22 nucleotides long, which functions in silencing gene expression. Changes in normal microRNA expression lead to cancer progression. The following study has been done to elucidate the changes in the expression of microRNAs in ovarian cancer compared to the normal expression because such comparison may lead t...
Article
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Mutations of spliceosome components are common in myeloid neoplasms. One of the affected genes, PRPF8, encodes the most evolutionarily conserved spliceosomal protein. We identified either recurrent somatic PRPF8 mutations or hemizygous deletions in 15/447 and 24/450 cases, respectively. 50% of PRPF8 mutant and del(17p) cases were found in AML and c...
Article
Recent application of whole exome next generation sequencing has led to the discovery of a new class of somatic mutations affecting various components of the spliceosomal machinery in myeloid neoplasms. All affected spliceosomal genes, except for LUC7L2 (which might function in the U1snRNP-related pathway) encode U2snRNP associated proteins, but th...
Article
Learning through trial-and-error interactions allows animals to adapt innate behavioural ‘rules of thumb’ to the local environment, improving their prospects for survival and reproduction. Naive Drosophila melanogaster males, for example, court both virgin and mated females, but learn through experience to selectively suppress futile courtship towa...