Amin Eimanifar

Amin Eimanifar
Universität Heidelberg · Institute of Pharmacy and Molecular Biotechnology (IPMB), Department of Biology

PhD in Biology and Natural Sciences - Main Subject: Evoloutionary genetic, phylogepgraphy

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67
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Publications

Publications (67)
Preprint
Full-text available
Due to the lack of a taxonomic key for the identification of Artemia species, molecular markers have been increasingly used for phylogenetic studies. The mtCOI marker is a regularly considered marker for the molecular systematics of Artemia populations. The proposed universal and specific primers have mostly failed to amplify the Artemia aff. sinic...
Preprint
Full-text available
Artemia is the most common live food which is used in aquaculture worldwide. This study reports on biometrical variation of introduced American Artemia franciscana cyst from 24 non-native localities and two native habitats in Asia and USA, respectively. Results showed the largest diameter of untreated cyst, diameter of decapsulated cyst and thicker...
Article
Full-text available
The traits of two subspecies of western honey bees, Apis mellifera scutellata and A.m. capensis, endemic to the Republic of South Africa (RSA), are of biological and commercial relevance. Nevertheless, the genetic basis of important phenotypes found in these subspe-cies remains poorly understood. We performed a genome wide association study on thre...
Article
Full-text available
In the previously published mitochondrial genome sequence of Artemia urmiana (NC_021382 [JQ975176]), the taxonomic status of the examined Artemia had not been determined, due to partheno�genetic populations coexisting with A. urmiana in Urmia Lake. Additionally, NC_021382 [JQ975176] has been obtained with pooled cysts of Artemia (0.25 g cysts consi...
Article
Full-text available
In the previously published mitochondrial genome sequence of Artemia urmiana (NC_021382 [JQ975176]), the taxonomic status of the examined Artemia had not been determined, due to parthenogenetic populations coexisting with A. urmiana in Urmia Lake. Additionally, NC_021382 [JQ975176] has been obtained with pooled cysts of Artemia (0.25 g cysts consis...
Article
Full-text available
Artemia franciscana, native to America, has recently colonized as non-indigenous population in Asia, Europe, North Africa, and Australia. We evaluated the effects of the colonization of A. franciscana on genetic differentiation in new environments in the United Arab Emirates (UAE). We used the COI marker to determine the genetic structure and origi...
Preprint
Artemia franciscana, native to America, has recently colonized non-indigenous populations in Eurasia, Mediterranean regions and Australia. In present we sought to evaluate the potential effects of colonization of A. franciscana on genetic differentiation in the new environments in UAE. We used the COI marker to determine population genetic structur...
Article
Full-text available
Due to the rapid developments in the aquaculture industry, Artemia franciscana, originally an American species, has been introduced to Eurasia, Africa and Australia. In the present study, we used a partial sequence of the mitochondrial DNA Cytochrome Oxidase subunit I (mt-DNA COI) gene and genomic fingerprinting by Inter-Simple Sequence Repeats (IS...
Article
Full-text available
The genus Artemia Leach, 1819 is a cosmopolitan halophilic crustacean, consisting of bisexual species and obligate parthenogenetic populations. Asia is rich in Artemia biodiversity. More than 530 Artemia sites have been recorded from this area and more than 20 species/subspecies/variety names have been used for them. There exist various problems in...
Article
Full-text available
The complete mitochondrial genome of the West African honey bee Apis mellifera adansonii consisted of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a control region. It was 16,466 bp and consisted of 84.7% AT nucleotides. This subspecies had a similar mitogenome to those of other southern African honey bees, namely A....
Article
Full-text available
The Spanish honey bee Apis mellifera iberiensis, had a mitochondrial genome of 16,560 bp. It consisted of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and an AT-rich control region. The sample was from Portugal and its mitogenome resembled those of the African (A)-lineage honey bee subspecies. It was most closely related to other North A...
Article
Full-text available
The mitochondrial genome of a worker Apis mellifera jemenitica was 16,623 bp. It consisted of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs and a control region. Phylogenetic analyses suggest a close relationship between A. m. jemenitica, A. m. lamarckii and A. m. syriaca.
Article
Full-text available
The mitochondrial genome of Apis mellifera ruttneri consisted of 13 protein-coding genes, two rRNAs, 22 tRNAs, an AT-rich control region, and was 16,577 bp long. The phylogenetic analyses suggested that A. m. ruttneri was closely related to two North African subspecies: A. m. sahariensis and A. m. intermissa.
Article
Full-text available
The complete mitochondrial genome of Apis mellifera simensis was 16,523 bp long. The 13 protein-coding genes, two rRNAs, and 22 tRNAs resembled other Apis mitogenomes. The location of this Apis subspecies in our phylogenetic tree supported the hypothesis that this subspecies is distinct, and is most closely related to A. m. scutellata and A. m. mon...
Article
Full-text available
Apis mellifera scutellata and A.m. capensis, two native subspecies of western honey bees in the Republic of South Africa (RSA), are important to beekeepers in their native region because beekeepers use these bees for honey production and pollination purposes. Additionally, both bees are important invasive pests outside of their native ranges. Recen...
Article
Full-text available
Urmia Lake, the largest natural habitat of the brine shrimp Artemia urmiana, has progressively desiccated over the last two decades, resulting in a loss of 80% of its surface area and producing thousands of hectares of arid salty land. This ecological crisis has seriously affected the lake's native biodiversity. Artemia urmiana has lost more than 9...
Article
Full-text available
Native American Artemia franciscana has become an introduced species in the Old World due to the rapid development of the aquaculture industry in Eurasia. The recent colonisation of A. franciscana in Mediterranean regions and Asia has been well documented, but Australia is a continent where the dispersal of this species is not well understood. In t...
Article
Full-text available
The taxonomic identity of an unknown Artemia population inhabiting the Al Wathba Wetland Reserve in Abu Dhabi, U.A.E., was determined using phylogenetic analysis of the mitochondrial marker Cytochrome Oxidase Subunit 1 ( COI ). The results showed that the examined population belongs to an exotic invasive species, Artemia franciscana . Based on the...
Article
Full-text available
The complete mitochondrial genome of Artemia sinica was obtained using the next-generation sequencing (NGS) method. The mitochondrial genome is a circular molecule of 15,689 bp in length, with the typical structure of 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs) and 2 ribosomal RNA genes, and a non-coding control region (CR). The b...
Article
Full-text available
Using two nuclear (ITS1 and Na+/K+ ATPase) and three mitochondrial (COI, 16S and 12S) markers, we determined the genetic variation and evolutionary relationship of parthenogenetic and bisexual Artemia. Our analyses revealed that mitochondrial genes had higher genetic variation than nuclear genes and that the 16S showed more variety than the other m...
Article
Full-text available
The brine shrimp Artemia urmiana, an abundant inhabitant of the hypersaline Urmia Lake in northwestern Iran, has recently been described from Lake Koyashskoe, also a shallow hypersaline lake that is located on the Black Sea coast of the Crimean Peninsula (Ukraine). This discovery has questioned the endemicity of A. urmiana in Urmia Lake and has als...
Article
The brine shrimp Artemia represents a widespread genus of microcrustaceans adapted to hypersaline environments. The species of this genus have been the subject of numerous phylogenetic studies, but many open questions remain, especially for Eurasian Artemia lineages. Artemia sinica Cai, 1989 and Artemia tibetiana have a restricted geographical dist...
Article
Full-text available
Asia harbors a diverse group of sexual and asexual Artemia species, including the invasive Artemia franciscana, which is native to the Americas. The phylogeny of Asian Artemia species and the phylogeography of the introduced A. franciscana from 81 sampling localities in Eurasia, Africa and America were elucidated using mitochondrial (COI) and nucle...
Article
The genetic structure of the brine shrimp Artemia franciscana, an extremophile halophilic microcrustacean, from the Great Salt Lake (USA), was investigated by analysis of nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I (COI), genomic fingerprinting by ISSR-PCR (inter-simple sequence repeats polymerase chain reaction) and bi...
Article
Full-text available
Urmia Lake, with a surface area between 4000 to 6000 km2, is a hypersaline lake located in northwest Iran. It is the saltiest large lake in the world that supports life. Urmia Lake National Park is the home of an almost endemic crustacean species known as the brine shrimp, Artemia urmiana. Other forms of life include several species of algae, bacte...
Article
We investigated the genetic variability and population structure of the halophilic zooplankter Artemia urmiana from 15 different geographical locations of Lake Urmia using nucleotide sequences of COI (mtDNA cytochrome c oxidase subunit I) and genomic fingerprinting by ISSR-PCR (inter-simple sequence repeats). According to sequence data, A. urmiana...
Article
Full-text available
Inland waters Artemia populations have specific biological characteristics, due to isolation pattern in their habitat environment. These populations mainly have different ionic compositions in the living environment which is actually described their biological characteristics. Owing to this reason, two bisexual species of Artemia from different geo...
Article
Urmia Lake is one of the two large hypersaline lakes in the world which have Artemia. It is located in northwest of Iran. Due to a decrease in water inflow and volume, the salinity of Urmia Lake has reached to more than 300 g.l -1 since 2001. The increased salinity has greatly influenced biological aspects of the lake, and caused the lake undergoes...
Article
Full-text available
Plants associations differ in relation to environmental gradients. Therefore, it is necessary to study the interaction between ecological factors and vegetation. In this study vegetation and flora of Sutan-Chay Basin in Arasbaran was investigated using Braun Blanquet method as physiognomic floristic. Sampling of soil and vegetation was carried out...
Article
Problem statement: Since there is no evidence to identify present species in the Bukan dam reservoir, therefore, this study was conducted to provide background for fisheries purposes. Approach: The abundance and species composition of ciliates were analyzed in the Bukan Dam reservoir (west Azerbaijan, Iran) from January to December 2007. Surface wa...
Article
Chitin and chitosan are both natural biopolymers that can be extracted from the shells (exoskeletons) of a variety of crustaceans. The objective of the present study was to characterize the chitin and chitosan obtained from the shells of Artemia urmiana cysts and to compare these to two commercially available products purified by chemical and biolo...
Article
Full-text available
Lake Urmia (or Ormiyeh) is one of the largest hypersaline lakes in the world and the habitat of a unique bisexual Artemia species (A. urmiana). Despite this, and several other values of the lake, little literature on it has been published. The present paper is an attempt to provide a brief review on various aspects of the lake. Urmia Lake, located...
Article
Full-text available
The live food enrichment technique using emulsions for preventing fish diseases was investigated as a tool for transferring therapeutics through the food chain. Nauplii and adult of the brine shrimp, Artemia urmiana were enriched with Oxolinic Acid (OXA) as an antibacterial drug to determine its content after different enrichment intervals. Various...
Article
Selectable marker genes (SMGs) have been extraordinarily useful in enabling plant transformation because of the low efficiency of transgene integration. The most used SMGs encode proteins resistant to antibiotics or herbicides and use negative selection, i.e., by killing nontransgenic tissue. However, there are perceived risks in wide-scale deploym...
Article
Genetic analysis using restriction fragment length polymorphisms (RFLPs) of cytochrome b in mtDNA was made to clarify genetic variations among two Iranian Rutilus rutilus caspicus populations of commercial importance from the southern coast of the Caspian Sea. Polymorphism was detected using six restriction enzymes and a total of six composite hapl...
Article
This study concentrated on the mitochondrial DNA diversity in adult Artemia urmiana populations. A total of 210 individual specimens were collected from the surface and bottom layers from three different geographical areas, comprising six sampling sites in Urmia Lake (Iran). The mitochondrial rDNA gene region was amplified using the PCR technique f...
Article
A rapid and reliable PCR-RFLP method was optimized to identify cyst batches of Artemia urmiana collected from different regions of Urmia Lake. Following DNA extraction, a 1564 bp region of a mitochondrial gene encoding the ribosomal RNA was successfully amplified by the PCR technique. Eleven restriction endonucleases were subsequently employed in o...

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Project (1)
Project
Current Project(s) DNA Barcoding identification of Salmo trutta fraio using Cytochrome Oxidase I (COI) ‎Sequences One of the main goals of establishing GenBank is providing the beast Preservation of aquatic genetic ‎resources and their biodiversity as national capitals by using biotechnological approaches. Collecting ‎biological samples of different aquatic species, preparation and long term storing, genetic recording ‎of threatened and under extinction species and using biotechnology methods for Conservation, ‎management and survival of aquatic species are other duty of GenBank of aquatic species of Iran. ‎