Amer Jamil

• PhD
• Professor at University of Agriculture Faisalabad

Proteases hydrolyze proteins and reduce them to smaller peptides or amino acids. Besides many biological processes, proteases play a crucial in different industrial applications. A 792 bp protease gene (nprB) from the thermophilic bacterium Streptomyces thermovulgaris was cloned and expressed in E. coli BL21 using pET 50b (+). Optimal recombinant p...

Ginger oleoresins being rich in coloring, flavoring and other phytochemical compounds have strong potential for industrial applications. In this study, 5 different oleoresins’ extraction methods are compared in terms of yield and phytochemical characterization of oleoresins. Petroleum ether exhibited the highest yield (5.51 ± 0.08%) among all selec...

CRISPR-Cas9, the “genetic scissors”, is being presaged as a revolutionary technology, having tremendous potential to create designer crops by introducing precise and targeted modifications in the genome to achieve global food security in the face of climate change and increasing population. Traditional genetic engineering relies on random and unpre...

Oleoresins are major coloring and flavoring components of herbs and spices. Among spices, garlic is a major spice used widely in cuisines and other food formulations all around the world. Garlic oleoresins have a promising future as functional ingredient in food industries. Apart from conventional extraction methods, by using modern extraction tech...

Begomoviruses are contagious and severely affect commercially important fiber and food crops. Cotton leaf curl Multan virus (CLCuMuV) is one of the most dominant specie of Begomovirus and a major constraint on cotton yield in Pakistan. Currently, the field of plant genome editing is being revolutionized by the CRISPR/Cas system applications such as...

Oleoresins are key flavoring and coloring ingredients in spices and herbs. Among spices, turmeric is most commonly used all around the world. Turmeric oleoresins have a promising future in pharmaceutical and food processing industries as functional ingredients. Apart from conventional extraction methods, by using modern extraction methods, a higher...

Methicillin-resistant Staphylococcus aureus (MRSA) has evolved resistance even against the last resort β-lactam antibiotics. This is because of the acquisition of an additional penicillin-binding protein 2a (PBP2a) which is a resistance determinant in MRSA. Currently, available PBP2a inhibitors are ineffective against life-threatening and fatal inf...

Antibiotics are becoming less effective as drug resistance spreads throughout the world, making it ever more difficult to treat the disease. The effect of biotic stress on antimicrobial activity of Lawsonia inermis L. (Henna) was studied as an alternate to antimicrobial agents against multidrug resistant microorganisms. The study was carried out in...

Peptides are macromolecules composed of few amino acids starting from a dipeptide structure to a polypeptide chain. Selection, isolation, and characterization of peptides with bioactive potential is the need of the day to combat against rapidly growing deteriorating agents affecting major areas of food, health, and agriculture. Peptides are pharmac...

In this study, small antimicrobial peptides of <10 kDa (AMPs) from Moringa oleifera L were separated from crude protein by Ultra-15 Centrifugal filter devices and partially purified. The potency of morintides to interfere with the virulence determinants like biofilms, siderophores and elastase of selected bacteria was investigated by spectrophotome...

Aims: Damage of crop plants due to pathogenic attacks, postharvest crops spoilage and lethal effects of chemical pesticides has enforced scientists to find some potential natural alternative. Plants produce different types of antimicrobial peptides including defensins in response to biotic and abiotic stresses. Defensins are small cysteine rich, ca...

A total of 60 isolates of Staphylococcus aureus, 30 each from upper respiratory tract as well as from post operative wound infections were subjected to antibiogram studies. Staph. aureus isolates from upper respiratory tract were found 30, 53.33 and 43.33% resistant against ciprofloxacin, amoxycillin and chloramphenicol respectively. A similar patt...

Reactive oxygen species (ROS) transform cell responses through miscellaneous processes. These act as signalling molecules when present in low concentrations or damage cell machinery when present in high levels. ROS-mediated mitochondrial damage leads to lesser oxidative phosphorylation and enhanced cell death. Aberrant cell death and redox signalli...

The emergence of the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (CRISPR/Cas) and its reengineering into a potent genome editing system has revolutionized life sciences. It has brought much excitement and hope in medical and agricultural research for unprecedented control over the redesigning of geno...

The current study was aimed to analyze the therapeutic effect of selected medicinal plants, that is, Curcuma longa, Zingiber officinale, Trigonella graceum-foenum, Nigella sativa, and Syzygium aromaticum against carrageenan-induced oxidative stress and inflammation in rats. Phytochemical analysis revealed the presence of diverse range of bioactives...

The revolutionary technology of CRISPR/Cas systems and their extraordinary potential to address fundamental questions in every field of biological sciences has led to their developers being awarded the 2020 Nobel Prize for Chemistry. In agriculture, CRISPR/Cas systems have accelerated the development of new crop varieties with improved traits—withou...

The revolutionary technology of CRISPR/Cas systems and their extraordinary potential to address fundamental questions in every field of biological sciences has led to their developers being awarded the 2020 Nobel Prize for Chemistry. In agriculture, CRISPR/Cas systems have accelerated the development of new crop varieties with improved traits—witho...

The present research project was designed to determine the genetic polymorphism of N-acetyltransferase 1 (NAT1), the acetylation status in the local Pakistani population because of the genetic polymorphism of drug-metabolizing enzymes constitutes an individual's susceptibility and toxicity for drugs. The normal health status of enrolled volunteers...

Background: Numerous diabetes susceptibility loci, include a region consisting vitamin D receptor gene found in chromosome 12q, have been known using genome wide screens. Aim: The aim of present study is to probe the relationship between polymorphism of vitamin D receptor gene (single nucleotide polymorphisms) and type 2 diabetes mellitus (T2DM). F...

Background: Numerous diabetes susceptibility loci, include a region consisting vitamin D receptor gene found in chromosome 12q, have been known using genome wide screens. Aim: The aim of present study is to probe the relationship between polymorphism of vitamin D receptor gene (single nucleotide polymorphisms) and type 2 diabetes mellitus (T2DM). F...

To cite this paper: Hussain, F., M.A. Sheikh, M.A. Maan and A. Jamil, 2012. Advanced glycation endproducts (AGEs) in diabetic patients with systemic lupus erythematosus. ABSTRACT Systemic Lupus Erythematosus (SLE), a systemic autoimmune disease has an increased risk for diabetes mellitus (DM) due to accelerated atherosclerosis. The accumulation of...

Background: The metabolism of several trace elements has been reported to alter in diabetes mellitus and these elements might have specific roles in the pathogenesis and progress of this disease. Objective: The aim of the present study was to investigate serum levels of copper, zinc, chromium, magnesium and manganese in type 2 diabetic patients and...

Cotton is an important natural fiber crop and is known as “white gold” due to its economic importance. Its seed is also a source of oil, proteins, and feed for the livestock. Sustaining cotton production has been a major challenge owing to the multitude of biotic and abiotic stresses. Among these, the infestation of insect pests and diseases are th...

Background: Numerous diabetes susceptibility loci, include a region consisting vitamin D receptor gene found in chromosome 12q, have been known using genome wide screens. Aim: The aim of present study is to probe the relationship between polymorphism of vitamin D receptor gene (single nucleotide polymorphisms) and type 2 diabetes mellitus (T2DM)...

Aenasius arizonensis (Hymenoptera: Encyrtidae) a nymphal, solitary endoparasitoid is a potent biocontrol agent of cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae). Female wasp injects a blend of secretions in its host during parasitization which alters the host physiology. Insecticidal potential of crude venom of A. arizo...

The main cause of hepatitis C is hepatitis C virus or HCV and for the cure of hepatitis C, NS3/4A protease has been found an important and emerging target. A number of HCV NS3/4A protease inhibitors have been discovered which have shown subsequent reduction in reducing the viral load leading to this infection however they are still undergoing clini...

To meet the food requirements of increasing human population in light of predicted climate change and extreme abiotic stress, crops with higher yield and enhanced abiotic stress tolerance are urgently needed. Previously, classical breeding and conventional genetic engineering led to limited success. However, these methods have several limitations,...

Bacterial proteases are of great pharmaceutical importance and have a key role in various biological processes and in life cycle of several pathogens. New technology used for rational protein engineering as well improved delivery options will expand the potential pharmaceutical applications of proteases. The catalytic proteases belong to metallopro...

The products industries will remain innovative in the current era of innovation and strive to produce goods and process products by handling their innovations correctly in order to succeed and stay profitable. In addition, products industries were also blamed for not having embraced product design attitude to boost business performance, while it is...

Stability and preservation of vitamin D are major concerns that lead to its encapsulation. Whey protein isolates (WPI) and soy protein isolates (SPI) were used as a carrier to effectively encapsulate vitamin D. The resultant microcapsules were evaluated for encapsulation efficiency, morphological characteristics, XRD diffraction, gastric release an...

COVID-19, a rapidly spreading new strain of coronavirus, has affected more than 150 countries and received worldwide attention. The lack of efficacious drugs or vaccines against SARS-CoV-2 has further worsened the situation. Thus, there is an urgent need to boost up research for the development of effective therapeutics and affordable diagnostic ag...

Worldwide, an estimated 130-170 million people are infected with Hepatitis C. The geographic distribution of HCV infection is highly variable between and within countries. To start the antiviral therapy, it is necessary to find the genotype in order to get better forecast about observed duration of treatment. This also provides the load of virus. T...

Longevity of probiotic is the main concern for getting maximum benefits when added in food product. Bifidobacterium, a probiotic, tends to lose its viability during gastrointestinal track (GIT) transit and storage of food. Their viability can be enhanced through microencapsulation technology. In this study, Bifidobacterium bifidum (B. bifidum) ATCC...

The main aim of the study was to establish the acetylation status of local population of Pakistan by N-acetyltransferase 2 (NAT2) enzyme and to find out the concordance between phenotypic and genotypic methods for the determination of NAT2 acetylation. Gender-wise comparison of selected healthy male and female volunteers aged greater than 18 years...

Globally, various methods of slaughtering have been practiced for the production of chicken meat. Among these methods, Halal slaughtering is implemented to produce Halal chicken. This method involves cutting the throat to bring the animal toward quick death without anguishing. Additionally, Halal slaughtering also favors rapid blood flow and more b...

In forensic science, the human identification is the major goal in criminal cases and in paternity dispute, based on DNA profiling of individual. Analysis of short tandem repeat (STRs) markers used as a reliable technique for this purpose. In this study the main objective was to develop a human identification based on DNA fingerprinting using Human...

Nanobiotechnology is emerging as a field of nanotechnology and applied biological science. Nanoparticles are produced by physical, chemical and biological methods; but biological methods are relatively cost effective, non-toxic, simple and eco-friendly. The present study was carried out focusing on the antimicrobial activities of phytofabricated si...

Humans are experiencing serious health issues like myocardial infarction and diabetes. Thrombosis is the reason of myocardial infarction that may cause death. Bioactive compounds or enzymes can be used to dissolve the clot. Whereas diabetes is a disorder of metabolism in which the level of glucose in blood becomes high. It can be controlled by inhi...

In pursuit of developing fast and accurate species-level molecular identification methods, we tested six DNA barcodes, namely ITS2, mat K, rbcLa, ITS2+mat K, ITS2+rbcLa, mat K+rbcLa and ITS2+mat K+rbcLa, for their capacity to identify frequently consumed but geographically isolated medicinal species of Fabaceae and Poaceae indigenous to the desert...

Sequences of matK in FASTA format

Sequences of rbcLa in FASTA format

matK+rbcLa combined sequences in FASTA format

ITS2+rbcLa barcoding gap backup file

ITS2+matK+rbcLa barcoding gap backup file

ITS2 sequences in FASTA format

ITS2+rbcLa combined sequences in FASTA format

NJ and ML phylogenetic trees for all barcodes with detailed captions

ITS2 barcoding gap analysis data

matK barcoding gap analysis data

Metadata, BOLD process IDs and GenBank accession numbers of specimens included in the study

Primers and PCR reaction conditions

Supporting data for Fig. 2 Species level discrimination ability of candidate barcodes by BM and BCM analyses

ITS2+matK combined sequences in FASTA format

matK+rbcLa barcoding gap backup file

ITS2+matK+rbcLa combination barcode in FASTA format

rbcLa barcoding gap analysis data

In pursuit of developing fast and accurate species-level molecular identification methods, we tested six DNA barcodes, namely ITS2, mat K, rbcLa, ITS2+mat K, ITS2+rbcLa, mat K+rbcLa and ITS2+mat K+rbcLa, for their capacity to identify frequently consumed but geographically isolated medicinal species of Fabaceae and Poaceae indigenous to the desert...

Present study investigated the effect of chemically (acid) modified starch on quality attributes of cookies. Cookies were supplemented with chemically modified starch at various levels (5, 10 and 15%) and their physicochemical and sensory properties were evaluated. The results showed that cookies substituted with varying levels of chemically modifi...

Nowadays, vegetables are gaining attention amongst the masses to mitigate lifestyle related dysfunctions owing to their safe nature and functional properties. Considering health benefits and cost-effectiveness of cabbage, the current research was designed to investigate the proximate composition and mineral profile of locally grown green and red ca...

In pursuit of developing fast and accurate species level molecular identification methods, we tested six DNA barcodes viz. ITS2, mat K , rbc La, ITS2+ mat K, ITS2+ rbc La, mat K+ rbc La, ITS2+ mat K+ rbc La for their capacity to identify frequently consumed but geographically isolated medicinal species of Fabaceae and Poaceae indigenous to the dese...

In pursuit of developing fast and accurate species level molecular identification methods, we tested six DNA barcodes viz. ITS2, mat K , rbc La, ITS2+ mat K, ITS2+ rbc La, mat K+ rbc La, ITS2+ mat K+ rbc La for their capacity to identify frequently consumed but geographically isolated medicinal species of Fabaceae and Poaceae indigenous to the dese...

Objectives Type-2 diabetes mellitus (T2DM) is an endocrine disease having a significant genetic component. Polymorphisms of many genes may affect hereditary vulnerability of the disease that is characterized by insulin resistance and islet disorder. As the genetic basis of T2DM can vary between ethnic groups, it is important to investigate the gene...

During the last decade, unprecedented progress in the field of genome modification has been witnessed with various applications in basic and applied biology. Genome editing with specific DNA binding proteins has shown higher specificity and fidelity. Artificially engineered proteins such as zinc fingers (ZFs), transcription activator-like effectors...

The present study was aimed at investigating the toxic effect of copper on seed emergence, seedling growth, chlorophyll contents and copper concentration in different parts of soybean [Glycine max (L.) Merr.]. Four soybean cultivars viz Walliam-82, NARC-2, Rawal-1 and Ajmeri-2104 were stressed with six Cu concentrations i.e., 50, 100, 150, 200, 250...

Antibacterial, antifungal and cytotoxic studies of two dimensional (2-D) cobalt coordination polymer[Co(3-PPA)2(H2O)2] (1) where PPA = pyridine propionic acid, has been evaluated. In 1, each cobalt metal ion bears slightly distorted octahedral geometry. The antimicrobial efficiency of the co-ordination polymer was tested on bacterial strains such a...

Terpenoids are synthesized in plants through the mevalonate (MVA) and the methylerythritol phosphate (MEP) pathways, among isopentenyl diphosphate (IPP) as the central intermediate metabolite. 3-Hydroxy-3-methylglutaryl- Coenzyme A synthase (HMGS) is a subsequent enzyme in MVA pathway of isoprenoid biosynthesis and catalyses the condensation of ace...

Hemorrhage is spontaneous bleeding caused by a defect in clotting mechanism which is fatal to human health. Proteases have important role in human health and are helpful in metabolism and in cure of diseases like hemorrhagic disorders. Proteases have a role in heamostasis mechanism in clot formation by the regulation and production of thrombin like...

Growth differentiation factor 11 (GDF11) is a member of the transforming growth factor-β (TGF-β) superfamily and acts as a regulator for the aging of multiple tissues. The mature forms of human GDF11 and GDF8 show more than 90% sequence identity but the role of GDF11 is not well understood. For detail annotation of GDF11 from Oryctolagus cuniculus,...

A remarkable diversity of extremophilic bacteria has been reported which has significant applications in biotechnology, environment, industry and medicine. Hot springs are good example of naturally occurring extremophilic environment. In our research, a catalase producing thermoduric alkalotolerant bacterial strain was isolated from the hot springs...

Chromomycin A3 and mithramycin are tricyclic antitumor compounds of aureolic acid class of polyketides, which are structurally related. Both polyketides differ in their glycosylation profiles and substitutions of sugars in their functional groups. Genetic organizations for the biosynthetic gene clusters of both polyketides were studied through anti...

Xylanases have got central importance in the utilization of lignocellulosic material in industry. Filamentous fungi have got tremendous importance for the production of xylanases. The expression of genes encoding the enzymes is regulated by different factors. The presence of readily metabolizable carbon sources like glucose, cellobiose, xylobiose,...

B-Cell Lymphoma protein-2 (BCL-2) is one of the most studied proteins with substantial regulatory potential for both apoptosis and autophagy. BCL-2 confer chemoresistance through influencing cancer pathophysiology. Serum level of lactate dehydrogenase (LDH) predicts increased anaerobic glycolysis and is associated with metabolic modulation in cance...

The current research was designed to assess the impact of storage on zinc retention and cooking & eating quality traits of zinc fortified super basmati rice. Purposely, sodium alginate and starch based edible coatings were introduced to retain zinc sulfate and zinc oxide, each @ 25 & 45 ppm within their matrix. Afterwards, the resultant coating for...

Xylan is the major component of hemicellulose in the cell wall of higher plants. The fungus Chaetomium thermophile produces extracellular xylanase when grown in the presence of complex polysaccharides. Xylanase is important enzyme that is used in food, paper and pulp industry. In the present work, xylanase was purified from Chaetomium thermophile b...

Lactoferrin, a multifunctional glycoprotein, occurring as awhey constitute in milk secretions of animals and humandisplays a variety of antimicrobial, antioxidant and immunomodulatory as well as a number of other biological functions. Its potential can be exploited in several food applications. Keeping in view the increased demand of natural antimi...

Objective: The treatment of hepatitis C virus (HCV) infection is rapidly evolving from interferon and ribavirin which is the standard of care (SOC) for genotype 3-infection, to the most effective Triple therapy by adding Sofosbuvir to the SOC regimen. We assessed the efficacy of Sofosbuvir based triple therapy in new and previously non-responder pa...

In the present study, differential expression of key DNA sequences responsive to salt stress in plant seedlings subjected to 150 mM NaCl, was investigated in a salt-sensitive canola line (Cyclone) by differential display reverse transcription PCR (DDRT-PCR). A canola cDNA fragment (SCA1H27) was isolated using an arbitrary primer that was up-regulat...

The present study aimed to evaluate the antioxidant activity of six selected aphrodisiac medicinal plants. Useful parts of the selected medicinal plants were collected and extracted in methanolic solvent. The antioxidant activity of selected plant extract was determined through different antioxidant assays, namely DPPH radical scavenging assay and...

A new complex [Co(phen)3]F2·2H2O has been synthesized and characterized by elemental analysis, FTIR studies, magnetic measurements, electronic absorption measurements and single crystal X-ray diffraction analysis. The crystals are triclinic in the P-1 space group, with a = 11.9520(8), b = 12.8152(9), c = 15.1013(11)Å, α = 98.175(2)°, β = 91.601(2)°...

Objectives: In last decade, “treatment of chronic hepatitis-C revolved frominterferon based therapy to most effective interferon free therapy with new direct antiviraldrugs like Sofosbuvir and ribavirin” which is recommended for all genotypes of HCV infection.Treatment response in Chronic Hepatitis-C is affected both by viral and human factors. Wec...

Salinity is one of the major factors which affect overall food production of the world, especially in arid and semi-arid regions. Present study was conducted to evaluate the effectiveness of four [two from chickpea (CRM-7 and CRM-9) and two from lentil (LMR-5 and LMR-10)] different strains of rhizobia individually as well as their consortium for re...

Qurs-e-afsanteen® has been reported to possess hepatoprotective potential, contains Artemisia absinthium (Afsanteen), Valerianaofficinalis (Sumbal-ut-tayyab), Rheum emodi (Ravendcheni), potassium nitrate and ammonium chloride. The present study was conducted to assess the protective effects of Qurs-e-afsanteen against gentamicin-induced liver toxic...

Objective: The main objective of the study was to determine the ratio of Apo-B/Apo-A1 in normal individuals and patients with metabolic syndrome. Study Design: Comparative / case control study. Place and Duration of Study: This study was conducted at the Department of Pharmacology, KGMC, Peshawar from June 2015 to December 2015. Materials and Metho...

Humans have relied on nature throughout their ages to cater for their basic needs including medicines to cure a wide spectrum of diseases. Plants have formed the basis for sophisticated systems of traditional medicines. For therapeutic agents many of the presently known lead compounds are natural products or their derivatives. Ethnomedicinal studie...

Antioxidants present in plants like secondary metabolites and peptides are gaining much interest while addressing the issues of oxidative damages to human and agriculture. The present study focused on evaluating the antioxidant potential of selected plants of indigenous sources including Viola odorata, V. tricolor, V. hybrid, pansy super hybrid F1,...