Amel Mohammed El Asely

Benha University · Department of Fish Diseases and Management

This study was conducted to determine the effect of replacing fishmeal (FM) with fermented soybean meal (FSBM) for 12 weeks on the growth performance, feed utilization, immunological parameters, antioxidant enzyme assays and lipid peroxidation, digestive enzymes, and histopathological analysis of juvenile Litopenaeus vannamei (L. vannamei). By subs...

In the current study, clinically diseased cultured Oreochromis niloticus at various life stages were collected from different fish farms within the governorates of Qalyubia, Kafr ElSheikh, Sharqia, and Port Said. The clinical pictures and gross lesions were recorded. Bacterial pathogen isolation and identification were accomplished using both tradi...

One of the most significant bacterial diseases affecting the Nile tilapia is streptococcus, which has a significant negative impact on the global economy. As a result, vaccination is seen to be the best strategy for controlling bacterial disease in farmed fish. The objective of the current study was to assess the effects of formalin and autoclaved...

This study was conducted to determine the effect of replacing fishmeal (FM) with fermented soybean meal (FSBM) for 12 weeks on the growth performance, feed utilization, immunological parameters, antioxidant enzymes assays and lipid peroxidation, digestive enzymes, and histopathological analysis of juvenile Litopeneaus vannamei (L. vannamei ). By su...

The use of phytobiotic substances as feed supplements in the aquaculture industry is generally increasing because of their safe and positive roles in promoting the performance, antioxidant, and immune responses of several aquatic organisms. In the current study, the stimulatory effects of dietary chia (Salvia hispanica) seed powder (CSP) on growth...

In the present study, the Amphora coffeaeformis diatom was applied in different concentrations to the diet of Nile tilapia (Oreochromis niloticus) to investigate its potential effects in improving growth performance, as well as enhancing immune and antioxidant responses to control Aeromonas hydrophila infection. The tilapia fingerlings were divided...

In the current experiment the effects of brown seaweeds (Sargassum aquifolium, SA) on blood biochemical, antioxidant, innate immune, and mRNA gene expression responses of Tilapia (Oreochromis niloticus) under high stocking rate were examined. The fish were allocated into equal six experimental groups: low stocking density (LSD) (the fish reared at...

The loach is one of the most important fish species in aquaculture in China. The study of peroxisome proliferator-activated receptors (PPARs) will be essential for the determination of the functional role of the PPARα and PPARγ in loach fish. We used immunohistochemistry for PPARα and PPARγ at some stages of development beginning from the age o...

This study was conducted in two experiments. Experiment 1 aimed to investigate the growth promoting and anti-oxidative effects of Azolla nilotica (AZN) and Spirulina platensis (SP) in Oreochromis niloticus. Seven fish groups (G1-G7), each in three replicates, were fed a basal diet (control), AZN 5%, AZN 10%, SP 0.5%, SP 1%, a mixture of Azolla nilo...

This study was conducted in two experiments. Experiment 1 aimed to investigate the growth promoting and anti-oxidative effects of Azolla nilotica (AZN) and Spirulina platensis (SP) in Oreochromis niloticus. Seven fish groups (G1-G7), each in three replicates, were fed a basal diet (control), AZN 5%, AZN 10%, SP 0.5%, SP 1%, a mixture of Azolla nilo...

Glyphosate, a common herbicide widely used all over the world, can reach the aquatic environment and adversely affect their living organisms. The current study was designed to investigate the possible ameliorating effect of Foeniculum vulgare (Fennel) against glyphosate exposure in Nile tilapia. The fish in this study were assigned into six groups:...

Using synthetic antibiotics/chemicals for infectious bacterial pathogens and parasitic disease control causes beneficial microbial killing, produces multi-drug resistant pathogens, and residual antibiotic impacts in humans are the major threats to aquaculture sustainability. Applications of herbal products to combat microbial and parasitic diseases...

Using synthetic antibiotics/chemicals for infectious bacterial pathogens and parasitic disease control causes beneficial microbial killing, produces multi-drug resistant pathogens, and residual antibiotic impacts in humans are the major threats to aquaculture sustainability. Applications of herbal products to combat microbial and parasitic diseases...

Here, we investigated the effects of total dietary fish oil (FO) substitution with plant oil (PO) on hematological indices, immune status, antioxidant activity, IL1β and TNF-α gene expression, and hypoxia stress resistance in Oreochromis niloticus at suboptimal temperatures. Fish (n = 360) were randomly divided into 12 circular fiberglass tanks (50...

Keywords The current investigation was undertaken to determine the potential causes of summer mass mortalities among farmed Nile tilapia (Oreochromis niloticus). Pure bacterial colonies were isolated from moribund O. niloticus from 13 different fish farms suffered from high mortalities ranged from (50-80%), during the period from April to October 2...

ARTICLE INFO ABSTRACT Keywords The current study aimed to control the bacterial infection associated with summer mortalities in Nile tilapia. The sensitivity of the isolated bacteria, including Aeromonas Veronii (A. veronii) strain (HY1, HY2, HY3, HY4 and HY6), Citrobacter freundii (C. freundii), Proteus vulgaris (P. vulgaris) and pseudomonas fluro...

In this study, Nile tilapia fingerlings with average body weight (8.6 ± 0.06 g) were exposed to zinc (Zn) toxicity and tested its amelioration with miswak (Salvadora persica L.) (SP) supplemented diet. Five fish groups were fed on diets with SP at 0, 0.25, 0.5, 1.0, and 2.0% (T1, T2, T3, T4, and T5, respectively) diet without Zn exposure, while ano...

This study evaluated the effectiveness of dietary Ziziphus mauritiana leaf powder (ZLP) to control Aeromonas hydrophila infection in Nile tilapia and reduce damage to vital immune organs. Four experimental groups were fed a diet supplemented with ZLP at concentrations of 0, 5, 10, and 20 g/kg (w/w) for 6 weeks. At the end of the feeding trial, all...

This study investigated the efficacies of honey bee pollen (HBP), ginger (Zingiber officinale), and Moringa oleifera leaves (MOL) as feed additives to counteract dimethoate (DM) toxicity in Nile tilapia (Oreochromis niloticus L.). The fish were divided into eight treatment groups: G1 (control group; basal diet + no DM exposure), G2 (2.5% HBP-supple...

Varied levels of Saccharomyces cerevisiae-fermented date palm seed meal (DSM) were included in Nile tilapia (26.95 ± 0.01 g) diets. Five diets were prepared to include fermented DSM at 0, 5, 10, 15 and 20 % and fed for fish for 8 weeks. The obtained results revealed that yeast fermented DSM significantly affected the final body weight, weight gain,...

Immunity occupies the top priority in aquaculture. Great efforts have been made to study fish immunity, but little is known about the immunity of grey mullet, Mugil cephalus despite of its importance. We evaluated the effect of different doses (0, 1, 10 and 100 µg/kg body weight) of lipopolysaccharide (LPS) on the expression of non‐specific immune...

The optimum water temperature required for the normal growth of Nile tilapia is 25–30°C. In this study, tilapia was reared under suboptimal temperature (21.50 ± 1.50°C) and fed four diets with fish oil (FO), corn oil (CO), sunflower oil (SFO) and linseed oil (LnO) for 8 weeks. The results revealed improved final weight, average daily gain and intes...

The current study evaluated the effects of dietary mannanoligosaccharide (MOS) on red sea bream (Pagrus major) performances. Fish fed five dietary levels of MOS at 0, 0.5, 1, 1.5 or 2 g/kg for 60 days. The results showed that MOS supplementation significantly increased the final body weight, weight gain, and specific growth rate with decreased feed...

Natural plant dietary supplementation in aquafeed could be used as an effective way for increasing the immunocompetency and disease resistance of fish. The current study aimed to investigate the potential effects of dietary Moringa oleifera leaf powder supplementation on the immune response, antioxidant status, and disease resistance against Aeromo...

Mercury (Hg) is an environmental pollutant that threatens aquatic life. Many environmental factors, including water temperature, are reported to influence the toxicity of dissolved chemicals in the aquatic ecosystem. Therefore, we investigated the impact of thermal stress on Hg-induced subchronic toxicity in Nile tilapia (Oreochromis niloticus). Fi...

This study aimed to assess the levels of pyrethroids and organochlorine residues in the tissues of cultured Mugil capito and in water samples obtained from three different sites (Al-Hamol, Al-Riad and Sidi Salem; referred to as Area 1, Area 2, and Area 3, respectively) in the Delta region, Egypt. The study also assessed the biochemical markers in e...

The aim of the present study was to examine the effects of dietary inclusion of fermented poultry by-product meal (FPBM) on growth performance, digestive enzymes activity, innate immunity, and antioxidant capacity in Nile tilapia (Oreochromis niloticus). A basal diet containing fish meal and soybean meal was considered as a control (Con), and four...

The current study investigated the effects of Moringa (Moringa oleifera) leaf meal (MLM) on growth and stress indices of Nile tilapia (O. niloticus). Experimental fish (2.0 ± 0.5 g average body weight) were divided into two groups; one was fed with a diet not supplemented with MLM (control diet), and the other group was fed with a diet supplemented...

The present study was conducted to investigate the effects of Ziziphus mauritiana leaf powder (ZLP) on growth performance, intestinal histomorphology and growth-related gene expression of Nile tilapia (Oreochromis niloticus). ZLP was incorporated into the basal diet at 5, 10 and 20 g per kg diets (w/w) and fed to fish for 12 weeks. The obtained res...

The aim of this work was to determine the anabolic and reproductive effects of antiestrogen (tamoxifen) in dietary supplemented Nile tilapia, Oreochromis niloticus of an average weight 5.30±0.2 g were fed four experimental diets containing (0, 25, 50 and 100 mg) tamoxifen/ kg food represented by; control, G1, G2 and G3 respectively for 90 days. Gro...

The aim of this study was to evaluate the relationship between fish size and expression of insulin like growth factor-1(IGF-1) in musculature and cytochrome P450 1A (CYP1A) in liver tissues using semi-quantitative PCR. Mugil capito with different sizes obtained from three different regions (Al-Hamol, Al-Riad and Sidi Salem) at Kafr El Sheikh Govern...

Clinically diseased Oreochromis niloticus, Claris gariepinus, and Mugil cephalus were collected from different localities at El-Riah El-Tawfiki and its tributaries (Qalyubia), Kafr El-Sheik and Ismailia governorates. The clinical picture and gross lesions were recorded. Isolation and identification of the bacterial pathogens using traditional and m...

The present study was carried out on two hundred Clarias gariepinus naturally collected during January to December (2016) to identify parasitic infestation affect C. gariepinus in downstream El-Rahawy drain, Giza, Egypt. The infested fish showed congested gills and fins, excessive mucous secretion, presence of yellowish white nodule in suprabranchi...

The present study was carried out on two hundred Clarias gariepinus naturally collected during January to December (2016) to identify parasitic infestation affect C. gariepinus in downstream El-Rahawy drain, Giza, Egypt. The infested fish showed congested gills and fins, excessive mucous secretion, presence of yellowish white nodule in suprabranchi...

The present study aimed to isolate and identify of Aphanomyces invadans from naturally infected African Catfish" Clarias gariepinus". A total number of 105 cultured Clarias gariepinus collected from private fish farms in Kafr Al-Sheikh & Behera Governorates. Result revealed that the prevalence of infection among the examined fish was 8.6 % and the...

The present study aimed to isolate and identify of Aphanomyces invadans from naturally infected African Catfish" Clarias gariepinus". A total number of 105 cultured Clarias gariepinus collected from private fish farms in Kafr Al-Sheikh & Behera Governorates. Result revealed that the prevalence of infection among the examined fish was 8.6 % and the...

The present study aimed to isolate and identify of Aphanomyces invadans from naturally infected African Catfish" Clarias gariepinus". A total number of 105 cultured Clarias gariepinus collected from private fish farms in Kafr Al-Sheikh & Behera Governorates. Result revealed that the prevalence of infection among the examined fish was 8.6 % and the...

The present study aimed to isolate and identify of Aphanomyces invadans from naturally infected African Catfish" Clarias gariepinus". A total number of 105 cultured Clarias gariepinus collected from private fish farms in Kafr Al-Sheikh & Behera Governorates. Result revealed that the prevalence of infection among the examined fish was 8.6 % and the...

The present study aimed to isolate and identify of Aphanomyces invadans from naturally infected African Catfish" Clarias gariepinus". A total number of 105 cultured Clarias gariepinus collected from private fish farms in Kafr Al-Sheikh & Behera Governorates. Result revealed that the prevalence of infection among the examined fish was 8.6 % and the...

The aim of the present investigation is to evaluate the effects of dietary ginger on haematological and biochemical parameters, spleen histopatholgical changes and disease resistance of Oreochromis niloticus (O. niloticus) fingerlings. Four test diets containing different levels of ginger (0, 1, 2 and 3%) (W/W) were fed for O. niloticus fingerlings...

Saprolegnia strain was isolated from infected goldfish,Carassius auratus, fingerlings. Fish showed extensive hyphal growth on the skin, fins, gills and eyes. The isolate was molecular identified through 18S rRNA gene and internal transcript spacer, ITS, sequencing. The isolate showed 18S rRNA gene nucleotide identity, 91.6%, and ITS homology with S...

A novel Saprolegnia parasitica strain was isolated from moribund and freshly dead g oldfish , Carassius auratus , fingerlings during mid February 2014 . Fish showed extensive hyphal growth on the skin, fins, gills and eyes as if it is warped with cotton. The isolate was identified using the traditional methods through its asexual and sexual...

This study was conducted on 210 Oreochromis niloticus (O. niloticus) of different life stages including (100 fry, 100 fingerlings and 10 broodstocks) obtained from a private fish hatchery at Kafer El-Sheikh Governorate, Egypt; during August 2014. The hatchery complains 30% mortality among fry and fingerlings while no mortalities was recorded among...

This study focuses on Anisakidosis as a disease caused by a number of Anisakid larvae including Anisakis simplex, Pseudoterranova decipiens, Hysterothylacium spp. and Contracaecum spp., through assessing the clinical signs and the clinical examination of the disease in 170 Lizard head (Saurida undosquamis) and 160 European hakes (Merluccius merlucc...

This study focuses on Anisakidosis as a disease caused by a number of Anisakid larvae including Anisakis simplex, Pseudoterranova decipiens, Hysterothylacium spp . and Contracaecum spp., through assessing the clinical signs and the clinical examination of the disease in 170 Lizard head ( Saurida undosquamis ) and 160 European hakes ( Merluccius mer...

This study focuses on Anisakidosis as a disease caused by a number of Anisakid larvae including Anisakis simplex, Pseudoterranova decipiens, Hysterothylacium spp. and Contracaecum spp., through assessing the clinical signs and the clinical examination of the disease in 170 Lizard head (Saurida undosquamis) and 160 European hakes (Merluccius merlucc...

The adverse effects of organophosphate dimethoate on testis histology and luteinizing hormone (LH) cells in the adenohypophysis of the Nile tilapia (Oreochromis niloticus) were examined. Adult male tilapias, average body weight 150±23.3 gm, were exposed to sublethal dose (5.00 mg/L-1) and environmentally relevant dose (0.03 mg/L-1) of dimethoate fo...

A novel Saprolegnia parasitica strain was isolated from moribund and freshly dead goldfish (Carassius auratus) fingerlings during mid February 2014. Fish showed extensive hyphal growth on the skin, fins, gills and eyes as if it is warped with cotton. The isolate was identified using the traditional methods through its asexual and sexual organs, fol...

Saprolegnia strain was isolated from infected goldfish,Carassius auratus, fingerlings. Fish showed extensive hyphal growth on the skin, fins, gills and eyes. The isolate was molecular identified through 18S rRNA gene and internal transcript spacer, ITS, sequencing. The isolate showed 18S rRNA gene nucleotide identity, 91.6%, and ITS homology with S...

Introduction: For the first time three- dimensional Computed Tomography was used in a study concerning Nile catfishes heads. Methods: The present study is carried out on 40 heads of four examplar species of catfish families, Bagridae (B. bajad); Clariidae (C. garpineus); Malapteruridae (M. electricus) and Mochokidae (S. schall). Specimens are doubl...

The present study was carried out on three freshwater fishes Oreochromis niloticus (wild and cultured), Clarias gariepinus and Lates niloticus which were collected from different localities from February to November (2013). The infested fishes showed no pathognomonic clinical signs except slight abdominal distention. Fish species subjected to paras...

The present study aimed at verifying the usefulness of dietary 2.5% bee-pollen (BP) or propolis (PROP) to overcome the genotoxic and endocrine disruptive effects of malathion polluted water in Oreochromis niloticus (O. niloticus). The acute toxicity test was conducted in O. niloticus in various concentrations (0–8 ppm); mortality rate was assessed...

The objective of this investigation was to study the total prevalence, seasonal Dynamics and diagnosis of Myxosporidiosis in different freshwater fishes. The present study was carried out on five fish species (wild and cultured) Oreachromis niloticus, Clarias gariepinus, Mugil cephalus, Lates niloticus and Ctenopharyngodon idella which were collect...

The objective of this investigation was to study the total prevalence, seasonal Dynamics and diagnosis of Myxosporidiosis in different freshwater fishes. The present study was carried out on five fish species (wild and cultured) Oreachromis niloticus, Clarias gariepinus, Mugil cephalus, Lates niloticus and Ctenopharyngodon idella which were collect...

To investigate the effects of Zingiber officinalis and Cyanodon dactylon supplementation on the growth performance and immune parameters of Macrobrachium rosenbergii juveniles, five experimental diets, including a control basal diet and four treated diets with 1.5 and 3% Z. officinalis ; 2 and 4% C. dactylon were prepared. Prawn fed with control an...

Immunostimulants are a category of the natural substances, which proved effective in controlling the disease in wide range of terrestrial animals and in the field of aquaculture. Plant mixed feed named MACH is a preparation made from four plants; namely, pumpkin (Cucurbita moschata) seeds, plantain (Plantago asiatica) seeds, honeysuckle (Lonicera j...

Nitric oxide (NO) signaling is involved in many physiological processes in vertebrates and invertebrates. In crustaceans, nitric oxide synthase (NOS) plays a significant role in the regulation of the nervous system and in innate immunity. Here, we describe the entire cDNA sequence (4616 bp) of the kuruma shrimp Marsupenaeus japonicus NOS (Mj NOS) g...

The present study was undertaken to investigate the effect of dietary administration of 17α methyl testosterone, letrozol and tamoxifen starting with the first exogenous feeding on sex ratio of Oreochromis niloticus. The survival rates were significantly decreased among fish treated with letrozol and tamoxifen and showed non significant variations...