Ambok Bolong Abol-Munafi

• PhD
• Professor (Full) at University of Malaysia, Terengganu

This study investigates the effects of phospholipids (PLs) supplemented diets on the growth, blood metrics, reproductive indices, and fatty acid profiles of male Pangasius nasutus (shark catfish) broodstock, a commercially valuable freshwater fish species. Over a 90-day feeding trial, four groups of fish were placed in a large open earthen pond wit...

Torsabo D, Ataguba GA, Iber BT, Noordin NM, Idris N, Koh ICC, Abduh MY, Piah RM, Abol-Munafi AB. 2024. Comparative investigation of biological indices and biochemical compounds of reproductive indicators in male and female Pangasius nasutus. Biodiversitas 25: 3105-3115. Pangasius nasutus (Bleeker, 1863) is an emerging freshwater aquaculture species...

Pangasius catfish, a significant player in the global whitefish market, encounters challenges in aquaculture production sustainability. Quality broodstock maintenance and seed production are impeded by growth, maturation, and fecundity issues. This review investigates the efficacy of strategic nutrient composition and molecular strategies in enh...

Buku ini mengumpulkan maklumat berkenaan biologi dan penternakan udang galah (Macrobrachium rosenbergii) secara lengkap untuk dijadikan rujukan bagi penternak, pelajar, penyelidik dan orang awam yang ingin memahami kaedah dan sains penternakan udang galah. Selain menerangkan pengurusan induk udang galah, penghasilan benih udang galah, pengurusan p...

This study explores the effects of phospholipid inclusions in the diet of Pangasianodon hypopthalmus young adults on growth and physiological parameters. Commercial diet was subdivided into four groups and top coated using phospholipid (food grade Soy Lecithin) at 0%, 2%, 6%, and 10%, of feed dry weight resulting in an equivalent of 5%, 7.39%, 11.9...

This study was aimed to describe the morphological appearances of gonopod and gonopores along with the carapace sizes at early crablet stage of mud crab, Scylla paramamosain. Changes in abdominal shape were recorded from instar stage 1 (Crablet 1 or C1) of the crabs for sexual differentiation. In addition, comprehensive measurements of their sizes,...

The origin of the probiotics, the age and size of the fish, the length, dosage, and technique of application, as well as the culture environment, affect the efficacy of probiotics. Juveniles of Tor tambroides (initial weight 2.53 ± 0.02 g) were used in the feeding experiment to compare the effects of host associated probiotics (HAPs) and commercial...

Ovarian lavage is a term used to describe the injection of fish with a catheter through the oviduct into the ovary. In this study, the efficacy of this technique was evaluated as a route for hormone administration and sperm preservation in the African catfish Clarias gariepinus. Firstly, the effects of hormone injection routes (namely, intramuscula...

Dietary lipid manipulation in the feed of commercially cultured finfish is used not only to improve production and culture but also to enhance their reproductive performances. The inclusion of lipid in broodstock diet positively affects growth, immunological responses, gonadogenesis, and larval survival. In this review, existing literature on the i...

This short note is published in the Malaysian Fisheries Journal vol 21, pg 22-25 (Fisheries Research Institute, Department of Fisheries Malaysia). The data in this article characterizes the carbohydrate profile of seasonal riverine fruits found in the diet of Malaysian mahseer. This study presented the carbohydrate constituents in the fruits; total...

Environmental factors affect the biological functions of aquaculture species both in the wild and in captivity. Therefore, optimization of the husbandry conditions under captivity can improve the production performance of an aquaculture species. In this study, the effect of water velocity on the glucose concentration, energy level of different body...

In an aquatic habitat, zooplankton organisms are an extremely valuable resource for aquaculture purposes. Potential zooplankton species (Ceriodaphnia cornuta) have been further explored to evaluate their applicability to fed fish larvae in freshwater aquaculture. There is a need to find more options other than Artemia as live food options in freshw...

Host associated probiotics (HAPs) provide health benefits to the host when administered as dietary supplement. However, a short-term probiotics application strategy has yet to be optimized. A 90-days study was conducted to evaluate the response of Malaysian mahseer, Tor tambroides post larvae fed with basal diet enriched HAPs for 30-days, and its r...

The study aimed to investigate the dietary supplementation effects of two strains of potential intestinal autochthonous probiotics, Enterococcus faecalis strains 2674 (T1) and FC11682 (T2), on growth performances, body composition, gut morphology of Malaysian mahseer, Tor tambroides, and survival of the juveniles challenged with Aeromonas hydrophil...

Sago (Metroxylon sagu), cassava (Manihot esculenta) and taro (Colocasia esculenta) are potential sources of carbohydrate in lieu of corn starch because of they can easily be grown as crops in Malaysia. We investigated the effects of these starch sources on the growth, feed utilisation efficiency, body composition, and liver and intestine histology...

Antinutritional components must be substantially reduced to ensure better utilization of unconventional feeds in animal nutrition. Among the different methods of processing, soaking represents a simple and inexpensive alternative. This study attempted to determine the nutritional properties of socked Jatropha curcas kernel (JCK) and the effect of i...

The nursery stages of mud crab, genus Scylla, proceed from the megalopa stage to crablet instar stages. We review the definition and several of the key stages in mud crab nursery activities. The practice of the direct stocking of megalopa into ponds is not recommended due to their sensitivity. Instead, nursery rearing is needed to grow-out mud crab...

The present research was conducted to investigate the effects of fish oil (FO) substitutions with corn oil (CO) on the gonad development of female Tor tambroides broodstocks, a high value freshwater fish. Three isonitrogenous (450 g kg−1 crude protein) and isolipidic (105 g kg−1 crude lipid) were formulated to contain FO and CO at a ratio of 1:0, 1...

This study was aimed at optimizing the induction of tetraploid in African catfish Clarias gariepinus eggs using cold shock protocol. Three temperatures (0, 5, and 10 °C) were applied at three durations (2, 5, 10 min) to suppress the first mitotic cell division at about 1.4 τ0 after fertilization. Thereafter, the treatment and control groups were in...

The study sought to investigate the chronology of events and timing of embryogenesis, as well as breeding performances of three strains of Heterobranchus longifilis from Nigeria. Fish samples were collected from Benue River in Makurdi, Niger River in Onitsha, and Rima River in Sokoto for this study. Induced spawning of the strains was carried out s...

This study was designed to examine the use of RAPD markers in discriminating triploid and diploid African catfish Clarias gariepinus (Burchell, 1822). Following a routine technique, triploidy was induced by cold shock and confirm by erythrocyte measurement in C. gariepinus. Thereafter, 80 RAPD markers were screened; out of which, three showed the h...

The nutritional value of Jatropha curcas kernel (JCK) can be improved through different processing methods; however, when using a thermal treatment, optimization of the process is needed to prevent denaturation of nutrients. In this study, JCK was toasted for varying durations (0, 10, 20, and 30 min) and nutritionally evaluated. The implication of...

The Malaysian mahseer (Tor tambroides) is a sport, ornamental and food all-in-one treasured aquaculture fish member of the Cyprinidae family. However, this freshwater riverine species is currently under the threats of habitat degradation and overfishing, apart from its poorly documented taxonomy. Despite its socio-economic significance, taxonomy co...

This study was carried out to determine the physiological changes (survival, growth, molting cycle, sex differentiation, and gill condition) of mud crab, Scylla paramamosain crablet at different water temperatures of 24, 28 and 32 °C, and ambient temperature of 27 to 30 °C. Thermoregulatory behavior, represented by preferred temperature (29.83 ± SD...

This study determined the effect of growth media and culture concentration on the growth, proximate, and microelement composition of Ankistrodesmus falcatus. The culture of A. falcatus was done using three media, namely Modified COMBO Medium (COMBO), Bold’s Basal Medium (BBM), and Bristol, at two concentrations (50% and 100%). The results obtained...

The body characteristics and yield indices of Clarias gariepinus, Bagrus bajad, Synodontis nigrita, Labeo senegalensis and Mormyrus rume from lower Benue River in Nigeria were determined in this study using 60 samples each for the fish species. Length, weight and fillet correlations were also determined during the study. Results obtained showed tha...

This study attempted to explain the definition, objectives, and key stages in mud crab nursery activities. The nursery stages of Scylla spp was started from megalopa stage to several crablet instar stages or started from earlier crablet stage to several crablet stages. Direct stocking of megalopa into ponds is not recommended due to their sensitivi...

The optimum distance and duration of ultraviolet (UV) irradiation for the complete inactivation of African catfish Clarias gariepinus egg nucleus was investigated in this study. The UV light was suspended above the unfertilized eggs at four distances (5, 10, 20 and 30 cm) and for five durations (1, 2, 3, 4 and 5 min). Then, the irradiated eggs were...

Despite having high economical value and declining wild population, the aquaculture of the Malaysian mahseer (Tor tambroides) is still way behind. Crucial information such as on its sexual dimorphism is scarce thus making its broodstock management in captivity challenging. The first step in studying fish sexual dimorphism is by observing and identi...

Anti-nutritional factors can be substantially reduced with processing to increase the utilization of feed ingredients. In this study, hydrothermally processed Jatropha curcas kernel (JCK) (62 min) was subjected to a natural un-inoculated fermentation process for varying durations (7, 14 and 21 days) to improve their nutritional profile and utilizat...

This study aimed to determine the effect of rearing water temperature on survival, growth and molting cycle of the mud crab, S. paramamosain from Megalopa up to 1st crablet instar (Crablet 1) and from Crablet 1 to 2nd crablet instar (Crablet 2). Different water temperature regimes were implemented i.e. 20 °C, 24 °C, 28 °C, 32 °C, 36 °C and ambient...

This study evaluated the effects of cooking for different hydrothermal-treatment durations (10, 20, 30 and 40 min) on the proximate composition, amino acid profile, fatty acid composition and organoleptic parameters of filets of African catfish Clarias gariepinus (Burchell, 1822). Filets of the fish were prepared from market size African catfish wi...

Aim: Methodology: Results: Interpretation: Key words: This study investigated the effects of enriched Moina micrura on growth, survivability, and body coloration of Betta splendens. Four enriched M. micrura treatments, namely M. micrura enriched with yeast (T1), Palm kernel cake (PKC) (T2), egg yolk (T3), and Chlorella sp. (T4), were tested for bes...

This study was designed to optimize the culture conditions of juvenile Epinephelus fuscoguttatus (Forsskål, 1775) under laboratory conditions. To this effect, the rate of oxygen consumption was monitored as an index of stress under different temperature, salinity, pH, photoperiod, and urea concentrations. The result obtained after 12 h of exposure...

The Labroides dimidiatus is known as the “doctor fish” because of its role in removing parasites and infectious pathogens from the body of other fishes. This important role played both in wild and captive conditions could represent a novel form of parasitic transmission process mediated by the cleaning activity of the fish. Yet, there is a paucity...

Discrimination of different fishes can be done through different means which includes morphological appearance. When two fishes are successfully hybridized, they produce progenies that have shared morphology between their pure parent, hence, making morphometric characterization an important aspect of hybrid discrimination. However, erythrocyte char...

Understanding the thermal tolerance of both candidate and potential aquaculture species is important for determining their temperature limit at certain stages of culture. The aim of the present work was to determine the effect of acclimation at different temperatures on the Critical Thermal Maxima (CTMax) and escaping temperature (Tesc) and Acclima...

Nutritional quality of the hepatopancreas and gonads of orange portunid mud crab, Scylla olivacea was evaluated for each gender under four treatment of different water velocities (0, 20, 40 and 60 cm s-1), in terms of nutrient reserve and nutrient for reproduction. About 56 crabs were used in this study in which fatty acids composition was analysed...

The enzyme glucose-6-phosphate dehydrogenase (G6PD) catalyses the metabolite glucose-6-phosphate in producing NADPH during the first phase of pentose-phosphate pathway thus provides reducing power to all cells for cellular growth, antioxidant defence, and biosynthetic reactions in all living organism. The deliberate inclusion of starch as carbohydr...

Failure to distinguish the sex of hatchery produced fish hinders Amphiprion ocellaris broodstock development in captivity as they need to be paired for breeding. Therefore, this study explored the possibility of producing male A. ocellaris broodstock by exposing the hermaphrodite juveniles to 17 -methyltestosterone (MT). Two sets of trials were co...

Aquaponics is known to be a smart way of producing fish and crops simultaneously; however, there is a paucity of information about the extents of this system's efficiency over other conventional methods of food production. Thus, this study was designed to evaluate the performance of a catfish–pumpkin aquaponics system in comparison with recirculato...

As water temperatures rise due to the effects of climate change, the effects of temperature on the physiological processes of aquaculture species must be studied. This study evaluates the effects of different water temperatures on the fatty acid (FA) composition and histological development of the gills in the blue swimmer crab, Portunus pelagicus....

This study investigated the use of electric-shock in inducing triploidy in African catfish Clarias gariepinus. To achieve this, three voltages (9, 12, 21 V) were applied for different durations (3, 5, 10 min). The shock was initiated approximately three minutes after fertilization followed by incubation in ambient temperature. After incubation, hat...

The present datasets were conducted to investigate glucose concentration in hemolymph, energy levels at selected body parts (hepatopancreas, muscle, gonad), and feces among different sexes of crabs cultured at four different water velocities (0, 20, 40, and 60 cm/s) during a 60-day culture period. A total of 102 immature crabs (51 males, and 51 fem...

The data collection was initiated to evaluate the effects of supplementary phospholipid to non-fishmeal based diet in order to make functional diets for the Malaysian Mahseer, Tor tambroides. Four iso-nitrogenous and iso-lipidic diets were formulated to consist 100% fishmeal (FM100), 0% fishmeal or full fishmeal replacement (FM0), and 0% fishmeal s...

This study was designed to determine the locomotor, escaping capability and fatty acids (FAs) composition of muscle Scylla olivacea mud crabs at different water velocities. Male and female immature S. olivacea were cultured at 0, 20, 40 and 60 cm/sin a recirculating marine aquaculture system. Increase in flow velocity increased the mean locomotor a...

The objective of the present study was to examine the effect of different rearing temperatures on growth, survival, instar development, and fatty acid (FA) composition of orange mud crab instars, Scylla olivacea. The experiment was conducted at four different water temperatures of 24 °C, 28 °C, 32 °C and 36 °C using instar stage 1 (C1) in temperatu...

Free-living nematode, Panagrellus redivivus, was tested as live food for blue swimming crab, Portunus pelagicus larvae during the first feeding stage. A series of experiments were conducted to determine the acceptability of the free-living nematodes in P. pelagicus larvae at first feeding. The optimum nematode density and the response of the larvae...

Survival and growth of blue swimming crab, Portunus pelagicus from instar 1 stage to instar 8 stage (C1 to C8), molting duration and time of hardening from instar C7 to C8 were examined at 24 °C, 28 °C and 32 °C. The carapace width decreased with increasing temperature, whereas the intermolt period also decreased. The inter-molt period of crab molt...

Water temperature is among the most important factors affecting reproduction and development of brachyuran crabs. The increase in mean global temperature resulting from climate change and atmospheric warming subjects all organisms to potential thermal stress. This paper reviews the effects of climate‐induce water temperature change on the thermal t...

Population density, growth, survival, water quality and larval stage index of purple mud crab, Scylla tranquebarica at different feeding regimes and the data on ingestion rate of chosen microalgae, survival and larval development of blue swimming crab, Portunus pelagicus are presented. A twenty days of S. tranquebarica larval culture from zoeal 1 u...

This study investigated the effects of sodium alginate supplementation on gut microbiota composition, health parameters, growth performances and growth‐related gene expression of Malaysian mahseer. Five test diets were formulated by supplementing 0%, 0.1%, 0.2%, 0.4% and 0.8% sodium alginate. Triplicate groups of juvenile Tor tambroides (2.19 ± 0.0...

The data collected in the present work correspond to the behavioral, Hepatosomatic Index (HSI), Gonadosomatic Index (GSI) and total lipid analysis between male and female mud crabs, Scylla olivacea at different water velocities. A total of 56 immature male and female crabs were used in this data article. The important criteria for estimating the se...

Temperature is an abiotic factor that affects various biological and physiological processes in fish. Temperature stress is known to increase the production of reactive oxygen species (ROS) that subsequently cause oxidative stress. Fish is known to evolve a system of antioxidant enzymes to reduce ROS toxicology. Glutathione peroxidase (GPx) family...

Malaysian Mahseer ( Tor tambroides ) is considered as a good prospect for aquaculture in Malaysia. However, knowledge about Malaysian Mahseer-associated sperm microbiota is still limited, although some studies reported that sperm-related bacteria are a factor in the decline of sperm quality, as sperm may become the carrier of pathogenic bacteria to...

This data article includes raw and analyze data for molting duration and time of hardening of blue swimming crab, Portunus pelagicus instar at three different temperatures of 24 °C, 28 °C, and 32 °C. Two sets of experimental data are included: first, the recorded on time-lapse video of duration of successful molted crabs (from emergence of swimming...

Gouramis require soft and acidic waters for optimal development, and in this context, a cheap and effective culture medium is required. In this study, Pearl and Blue gourami larvae were raised in Terminalia catappa leaf extract (TCE), clear water, and green water, the latter containing 1 × 10⁵ cells/ml Chlorella from Tilapia cultures. Larval surviv...

This article investigated how crabs responded to different culture temperatures especially dislocation before molting using a combination of large recording files and computer software. In this novel approach of video recording portunid crab behavioral data, crab culture was recorded at five different acclimation temperatures of 20, 24, 28, 32 and...

Many crustacean species including mud crab, genus Scylla are incapable of natural maturation under captivity, thus putting high pressure on the wild catch. Therefore, to increase the availability of mature broodstocks in captivity, this study determined the effect of Eyestalk Ablation (EA) and Methyl Farnesoate (MF) administration on ovarian matura...

This study was investigated to examine supplemental effects of dietary inosine monophosphate (IMP) on growth performance, feed utilization, biochemical, hematological and immunological parameters of juvenile Nile tilapia Oreochromis niloticus. Disease resistance to experimental infection with Streptococcus agalactiae was also assessed. A semi-purif...

Owing to its potential market value, the blue swimmer crab Portunus pelagicus is of great economic importance. The temperature of water significantly affects the physiological function and production efficiency of these crabs. The aim of the present study was therefore to examine the critical thermal minimum (CTMin), critical thermal maximum (CTMax...

The aim of this study was to investigate how various diets influence testis maturation stages in mud crab (Scylla olivacea) broodstock. Morphological and histological assessments were performed in triplicate (10 male crabs each). Daily, subject crabs were fed a squid (Loligo sp.) and a fish (Decapterus sp.) diet at 5-10% of body weight. Diets were...

A 60-day feeding trial was conducted to study the effect of partial replacement of fishmeal with palm kernel meal (PKM) on growth performance and oxidative stress of Malaysian prawn, Macrobrachium rosenbergii. A closed aquaculture system with 21 fibreglass tanks with the capacity of 150 liter was used for the experiment. Five iso-energetic (19 KJ/g...

In modern aquaculture, dietary supplementation of probiotics is a novel approach for enhancing growth performance of slow-growing fish. However, the actual role of probiotics in regulating muscle growth at cellular and molecular levels in fish still needs to be clarified. In this study, we hypothesized that host gut derived probiotic bacteria would...

Three host-associated probiotics (Bacillus sp. AHG22, Alcaligenes sp. AFG22, and Shewanella sp. AFG21) were isolated from the gastrointestinal tract of Tor tambroides, and their effects were evaluated on gut morphology, microbiota composition and volatile short chain fatty acids (VSCFAs) production of the same species. A control diet (40% crude pro...

Studies on the climate change have attracted major scientific interests. The purpose of this editorial letter is to interpret recent issues related to the portunid crab aquaculture activities, and then to discuss the importance of crab aquaculture, and finally the challenges posed by climate change. It is well known that aquaculture is fast becomin...

Microalgae are natural producers of various biochemical compounds such as protein, lipid, carotenoids and fatty acids. However, the biochemical composition of microalgae can vary depending on species, culture conditions as well as composition of culture media. This research aims to compare the growth and proximate composition of Scenedesmus obliquu...

A 60-day long feeding trial was conducted to investigate the effect of various levels and sources of dietary phospholipids (PL) on growth, survival, feed utilization, fatty acid composition and oxidative status of juvenile Tor tambroides. Seven diets were formulated to contain one control group (PL0), three levels of soybean lecithin (PL2%, PL4%, P...

Several scientific methods have been described in the identification of triploid fish. However, many of these methods are not applicable for routine management purposes due to their complexity and cost. In this study, the possibility of using morphological variation as a least cost and less complex method of distinguishing triploid and diploid Afri...

The social structure of the false clownfish (Amphiprion ocellaris) typically consists of a pair of functional brooders within a population of individuals which remain as protandrous hermaphrodites usually incapable of breeding. This situation often hampers the commercial scale production of large numbers of clownfish offspring and broodstock. To en...

Isolated compounds from marine invertebrates are being increasingly known to possess various pharmacological activities with which many useful drugs have been developed. Crabs contain bioactive compounds including antibacterial, antifungal and antiviral metabolites, isolated from various tissues and organs that have revolutionized treatment of seri...

Environmental factors, growth phase and microalgal strain have been reported to influence the chemical composition of microalgae. Salinity is one of the important culture conditions that can influence growth and proximate composition of microalgae. Dunaliella sp. is a commercial microalgae species that has been widely used in various sectors due to...

Background and Objective: Proper knowledge on reproductive biology is important for the successful management of fisheries, aquaculture and conservation of a target species in the wild. The present study was investigated to uncover the reproductive biological information including the spawning season of Xenentodon cancila . Materials and Methods: A...

The influences of live and formulated feeds on growth performances, muscle fibers morphometry and muscle growth-related gene expression of Malaysian mahseer, Tor tambroides larvae were evaluated in indoor nursery rearing system. Quadruplicate groups of T. tambroides larvae (0.07 ± 0.01 g, mean ± SE) were stocked in sixteen aquaria (60 × 30 × 30 cm)...

This study was aimed to determine the ovarian maturation stages of wild and captive orange mud crab, Scylla olivacea fed with different diets via gonadosomatic status, oocyte diameter and histological examinations. Captive crabs were fed with blood cockle, Anadara granosa, or fish, Decapterus spp. Through the histological examinations, ovarian matu...

Mud crab (Scylla sp.) aquaculture has gained in popularity due to diminishing wild stocks and high market demand. However, the industry is facing a shortage in quality formulated feed and research on practical feed development remains scarce. We conducted a 2×2 factorial experiment to examine the interactive effects of different levels of fishmeal...

Mud crab, Scylla sp. aquaculture has gained in popularity due to diminishing wild stocks and high market demand. However, the industry is facing a shortage in quality formulated feed and research on practical feed development remains scarce. We conducted a 2 × 2 factorial experiment to examine the interactive effects of different levels of fishmeal...

The consequences of live and formulated feeds on carcass biochemical composition and growth performances of Malaysian mahseer, Tor tambroides larvae were assessed in indoor nursery rearing systems. Quadruplicate groups of T. tambroides larvae (0.07 ± 0.01 g, mean ± SE) were stocked in sixteen aquaria (60 × 30 × 30 cm), randomly arranged in four die...

This study describes the digestive enzyme profiles from foregut contents of the blue swimming crab, Portunus pelagicus (L.), from Straits of Johor, Malaysia. Adult male (mature and immature) and female (ovarian maturation stages 1–4) blue swimming crabs were assayed for the presence of digestive enzymes (protease, trypsin, amylase and lipase) in th...

This study was aimed to determine the ovarian maturation stages of wild and captive orange mud crab, Scylla olivacea via morphological observation, histological study and fatty acid composition examinations. Samples were collected from the natural habitat of Kedah Coastal Water, Malaysia from May to July 2011. Captive crabs were treated with two di...

Muscle growth mechanisms are controlled by different cellular and molecular pathways, and dietary supplementation of nucleotides (NTs) may play an important role to influence such pathways in fish. In this study, we hypothesized that dietary supplementation of inosine monophosphate (IMP) would enhance cellular muscle growth, and upregulate growth-r...

Objective: A study was done to characterize the erythrocyte of triploid African catfish. Methodology: Triploid African catfish Clarias gariepinus fingerlings were produced via cold shock method and their erythrocyte profiles were compared with normal diploid controls. Results: Approximately 99% of triploid erythrocytes were ellipsoidal whereas 97.5...

The human demand for fish protein is increasing daily with continuous growth of the global population and economy. Due to the low harvests of wild-caught fish in conjunction with high human fish consumption, aquaculture presents the most effective solution for enhancing the production of fish protein. However, most fish and shellfish hatcheries ful...

Electroshock had been widely used for scientific studies on riverine fish. The present study was carried out on the bacteriological and psychochemical quality of climbing perch (Anabas testudineus) that subjected to different voltages of electroshock (50, 70, 90 and 110 V) with controls left untreated. Electric charge at 70-90 V gives significant b...