Ambikanandan Misra

The Maharaja Sayajirao University of Baroda | MSU · Department of Pharmacy

The application of drug delivery is a valuable, cost-effective lifecycle management resource. By endowing drugs with new and innovative therapeutic benefits, drug delivery systems extend products’ profitable lifecycle, giving pharmaceutical companies competitive and financial advantages, and providing patients with improved medications. Formulation...

The management of wounds and burns is becoming difficult using conventional therapeutics available due to resistance development by microbes. Therefore, there is an utmost need to develop therapeutic alternatives to these agents. Antimicrobial peptides have emerged as a novel class of agents for the effective management of wounds and burns due to t...

Aim of this study was to develop anti EGFR antibody conjugated poly (lactide-co-glycolide) nanoparticles (NPs) to target epidermal growth factor receptor, highly expressed on non-small cell lung cancer cells to improve cytotoxicity and site specificity. Cetuximab was conjugated to Docetaxel loaded PLGA NPs by known EDC/NHS chemistry and characteriz...

The aim of study was to formulate self-microemulsifying drug delivery systems for modafinil. It was also an objective to optimize formulation parameters and evaluate developed formulation for its in vitro, ex vivo and in vivo performances. Solubility of modafinil was determined in various vehicles (oil, surfactant and co-surfactant) and selected ex...

Apart from the treatment of local diseases, nasal drug delivery has caught attention of formulation scientists since long for direct delivery to brain as well as systemic delivery not only of small drug molecules but also of proteins and peptides. The classical advantages of nasal drug administration, such as ease of local treatment, enhancing bioa...

Background: The development and progression of atherosclerosis is known to occur at a sluggish pace and the lesions remain concealed for a long duration before the factual situation of the complex atherosclerotic etiology affecting various organ gets apprehended. The root cause mainly involves an imbalance or malfunction of the cholesterol metabol...

A number of formulations have been developed and reported in literature as a carrier for drug, gene, and diagnostic agents. Amphiphilic block copolymers have achieved increasing attention due to their high stability both in vitro and in vivo and their good biocompatibility. In comparison to other long-circulating nanocarriers, micelles possess a nu...

The present studies were designed to develop a microemulsion (ME) formulation of Amphotericin B (Amp B) for the treatment of invasive fungal infections. The oil phase was selected on the basis of drug solubility whereas the surfactant and co-surfactant were screened and selected on the basis of their oil solubilizing capacity as well as their effic...

The vast majority of pathogens enter the human body via the mucosal surfaces of the gastrointestinal, respiratory, and urogenital tracts, where they initiate mucosal infections that lead to systemic infections. Despite strong evidence that a good mucosal immune response can effectively prevent systemic infection too, only a few mucosal vaccines are...

Introduction: Over the past decades, proteins have emerged as versatile carriers for the diagnosis and treatment of cancer, diabetes, rheumatoid arthritis, and many more diseases. Proteins have gained considerable attention in formulation of several delivery systems for anticancer drugs due to their nontoxic, non-immunogenic, biocompatible and bio...

The epidermal growth factor receptor (EGFR) is a cell-surface receptor belonging to ErbB family of tyrosine kinase and it plays a vital role in the regulation of cell proliferation, survival and differentiation. However; EGFR is aberrantly activated by various mechanisms like receptor overexpression, mutation, ligand-dependent receptor dimerization...

RNA interference (RNAi) is a wondrous phenomenon that silences the expression of targeted genes via distinct messenger RNA degradation pathways. It has the potential as a therapeutic agent for variety of diseases, including viral infections, cancer, and immune diseases. RNAi mainly conducts gene regulation by 3 ways: microRNA, short hairpin RNA, an...

Pulmonary arterial hypertension (PAH) is a progressive disease of the pulmonary vascular system, which may lead to right-heart failure or early death in the absence of effective treatment. The current therapy for PAH mainly includes phosphodiesterase inhibitors, prostanoids and endothelin receptor antagonists. These, however, have adverse effects w...

siRNA inhibits protein expression by degrading complementary mRNA sequence and hence, it is widely applicable for the treatment of various diseases where single or multiple gene knock down is necessary. Due to the severity and lethality of pulmonary diseases, siRNA has been focused for improved health in these diseases. Pulmonary accumulation of si...

This chapter focuses on the delivery of DNA by various physical methods such as electroporation (EP), sonoporation, microinjection, particle bombardment (gene gun), and hydrodynamic injection with the application of external physical force (pressure, sound, shock wave, and electric pulses for efficient gene transfer inside organs and cells. The cha...

This chapter focuses on protein and peptide delivery through the respiratory pathway. In pulmonary delivery of peptides and proteins, particle deposition and absorption are influenced by the aerodynamic size of the inhaled particles, resistance to airflow, dissolution, partition and diffusion of drug particles in respiratory tract fluids, and stabi...

Safe and efficacious gene delivery requires a suitable vector. The role of viral vectors in gene delivery is primary due to their function in the delivery of genetic material into host cells. However, the major problem in the use of viral vectors in gene therapy is immunogenic reaction risks and short-lived expression, which is of utmost importance...

This chapter outlines the progress made to meet delivery challenges and the clinical applications of antisense technology and highlights the limitations of antisense agents in therapeutics. Antisense technology presents an opportunity to manipulate gene expression within cells to treat an endless number of diseases and is a powerful tool for studyi...

Gene therapy is a clinical therapeutic procedure in which genes are intentionally introduced into human somatic cells for turning on or restoring a gene function (gain of function) to produce a therapeutic protein with the desired action. Successful gene therapy requires a convenient and patient-acceptable route of gene administration, control over...

Gene therapy is a powerful tool for the prevention and cure of diseases as it aims to treat the cause rather than the symptoms of the diseases. Gene therapy is defined as the correction of dysfunctional or deleted genes by supplying the lacking component as a means to permanently treat or reverse diseases. There are a number of human diseases, both...

This chapter provides a general overview of proteomics, and its importance in studying the role of proteins in a biological system. Proteomics involves the large-scale study of proteins, their structure, and their physiological role or functions. Proteins are quintessential cellular components or biomolecules in any living organism. Proteomic studi...

DC- encapsulated solid lipid nanoparticles (SLN) were prepared using Supercritical fluid technology using CO2 as an anti-solvent. RGD-conjugation was done by carbodiimide coupling method. The SLN were characterized for particle size and zeta potential by zeta sizer (Nano-ZS), entrapment efficiency and in-vitro drug release profile using HPLC assay,...

Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC Aim of the studies was to investigate influence of targeting (RGD) moiety on biodistribution of Docetaxel (DC) encapsulated PLGA-NPs in tumor bearing rats. Nanoparticles (Nps) were prepared by emulsification technique. RGD-conjugation was done by carbodiimide method. NP...

The purpose of the study was to prepare, characterized, assess in vitro antiproliferative activity, and determine DNA content and mode of cell death of RGDfk grafted Docetaxel encapsulated PLGA nanoparticles on breast cancer cells. DC- encapsulated nanoparticles were prepared using solvent evaporation technique. RGD-conjugation was done by carbodii...

Publisher Summary This chapter focuses on developments in the injectables for peptide and protein (P/P) delivery to improve their therapeutic response and reduce their side effects and immunogenicity, and on the relevant aspects of formulating the therapeutic protein in a suitable delivery system. P/P drugs are currently administered by the parente...

BACKGROUND AND THE PURPOSE OF THE STUDY: Itraconazole is a poorly water soluble drug which results in its insufficient bioavailability. The purpose of the present study was to formulate Itraconazole in a nanosuspension to increase the aqueous solubility and to improve its formulation related parameters, dissolution and hence oral bioavailability. I...

Since the Nobel prize-winning discovery of RNA interference (RNAi) in 1998, considerable money has been invested in the investigation of therapeutic potential of RNA interference (RNAi) and its application in human diseases. Encouraging results of ongoing clinical trials for the treatment of age-related macular degeneration and respiratory syncytia...

Using "click chemistry" as an easy and versatile synthetic strategy to combine hyaluronan and polyglutamate blocks, we have prepared nanovesicles (polymersomes) that present a controlled size, excellent colloidal stability, and a high loading capacity for hydrophilic and hydrophobic drugs. The unique feature of our concept is the use of hyaluronan,...

The aim of the present investigation was to improve in vitro pulmonary deposition of amphotericin B (AMB) liposomal dry powder inhaler (LDPI) formulations. Liposomes with negative (AMB1) and positive (AMB2) charge were prepared by the reverse phase evaporation (REV) technique, extruded to reduce size, separated from unentrapped drug and lyophilized...

The findings of this investigation thus conclusively demonstrate the promising role of concomitant therapy of liposomal TRE and BP gels in treating acne patients. Allthough their use improved the therapeutic response, more benefits of the liposomal form of drugs were observed in terms of reduction in adverse effects of therapy, thus fostering bette...

An attempt was made to provide cheaper, indigenous, directly compressible lactose by thermal modification of lactose I.P. The modified lactose (Thermally modified lactose, TML) was characterised for physico-chemical properties and flow behaviour and the results compared with those of Marketed directly compressible lactose (MDL).The direct compressi...

Block copolymers are a well-known and representative class of macromolecules that have been synthesized in laboratory during the last fifty years. Recent progresses in the synthetic strategies that can be applied to vary the chemical composition and architecture of block copolymers open new areas of development. An important issue towards making th...