Ambekar Eknath

Ambekar Eknath
E-Biotech

PhD

About

77
Publications
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2,263
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Publications

Publications (77)
Article
Selection for increased body weight at harvest in Nile tilapia (Oreochromis niloticus) was carried out for five generations from 1991 to 1996, as a part of the project Genetic Improvement of Farmed Tilapias (GIFT). The base population for selection was composed of a mixture of various three- and four-way cross individuals descending from four wild...
Article
Full-text available
Community based aquaculture, a subset of the rural aquaculture, is characterised with low fish production level. Sustainable improvement of productivity levels in such resources has remained a challenge. The study adopted a novel approach of technology transfer through aquaculture field school which involves provision of technical advices and no ph...
Article
The reproductive biology of the Indian Medium Carp (Puntius pulchellus) was studied and induced breeding of the fish accomplished. Males were dark and exhibited pinkish red prominent tubercle's on the snout in contrast to the plain snouted females which however, had a deep pink lateral band. The gonado somatic index observed in females indicated a...
Chapter
Investigation on fish disease surveillance was carried out in randomly chosen 81 village community ponds of Khurda and Puri districts, Odisha during 2009-2011. Unlike private fish farms, community fish farming entail an approach, where large numbers of stakeholder's benefits are upheld properly. In the study over 60% of the total freshwater ponds a...
Article
The aim of this study was to evaluate dietary dosages of Microcystis on the immune response and disease resistance against infections due to the opportunistic pathogen Aeromonas hydrophila in Labeo rohita fingerlings. Labeo rohita fingerlings were fed diet containing 0 (Control), 0.5, 1.0 and 5 g Microcystis powder kg−1 dry diet for 90 days. Three...
Article
Labeo rohita (Ham.) also called rohu is the most important freshwater aquaculture species on the Indian sub continent. Monsoon dependent breeding restricts its seed production beyond season indicating a strong genetic control about which very limited information is available. Additionally, few genomic resources are publicly available for this speci...
Article
Labeo rohita (139.92 ± 0.76 mm/24.33 ± 0.45 g) was reared for 92 days in floating square cages (10 m2 area, 1.5 m height) in a pond (2 ha) at six stocking densities (5, 7.5, 10, 15, 20 and 25 fish m−2) each with 3 replicates. Fish were fed daily once in the morning with rice polish and groundnut oil cake (1:1) in dough form at 3 % of the total body...
Article
Full-text available
Carcass and commercial yield traits of Pangasius pangasius were evaluated and morphometric parameters measured along with carcass traits in two marketable weight classes (average ± SD) 1603 ± 49.5 g and 1985.6 ± 56.6 g. The Pangas fish head yield was 14.80% and 15.1% for the 2000 g and 1600 g weight classes, respectively. The offal percentage varie...
Article
The crude ethanol, methanol and aqueous extracts of Spirulina (Spirulina platensis) have been investigated for antibacterial activity using disc diffusion assays against various strains of fish and shellfish pathogens e.g. Pseudomonas putida (ATCC 49128; PP1, PP2), Pseudomonas aeruginosa (ATCC 35032; PA1, PA2), Pseudomonas fluorescens (PF1, PF2), A...
Article
Expressed genes and polymorphisms were identified in lines of rohu Labeo rohita selected for resistance or susceptibility to Aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis. All animals were grown in a common environment and RNA from ten individuals from each line pooled for Illumina mRNA-seq. De novo transcriptome assemb...
Article
Culture of freshwater climbing perch, Anabas testudineus Kuldeep Kumar, U,. Mohanty, R. Kumar, D.Damle, Noor Jahan, J. K. Jena and A. E. Eknath Aquaculture Production and Environment Division, Central Institute of Freshwater Aquaculture (ICAR), Bhubaneswar-751 002, Orissa, India The climbing perch, Anabas testudineus is one of the important air-bre...
Article
Full-text available
Partial purification of amylases from the digestive tract of the Indian medium carp Labeo fimbriatus through acetone fractionation and Sephadex G-75 gel filtration resulted in 5-fold purification with 29% recovery. Characterization of amylase activity revealed two pH optima at 4.5 and 6.5-7.0. Activity was stable over a wide pH range of 4.0-10.0. O...
Article
Full-text available
Vibrio alginolyticus and V. parahaemolyticus were characterized by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and profiling of outer membrane proteins (OMP) to the genetic level to enable easier identification of these microorganisms from diseased shrimp (Penaeus monodon) and adopt control measures. Of 20 tested Operon pr...
Article
Full-text available
In the present study the haemolytic and proteolytic activity of extracellular products (ECP) secreted from Aeromonas hydrophila (CAHH14 strain) were studied with respect to temperature and different time of incubation as well as its lethal toxicity on rohu, Labeo rohita. The strain was isolated from Catla catla (showing abdominal dropsy symptom) co...
Article
Nucleotide-binding and oligomerization domain (NOD)-2 is a cytoplasmic pattern recognition receptor (PRR) and is a member of NOD like receptor (NLR) family. It senses a wide range of bacteria and viruses or their products and is involved in innate immune responses. In this report, NOD-2 gene was cloned and characterized from rohu (Labeo rohita) whi...
Article
Interleukin-10 (IL-10) is a multifunctional cytokine and plays an important role in diseases. In this study, IL-10 gene was cloned and characterized from catla (Catla catla), which is a highly commercially important fish species in the Indian subcontinent. The result indicated that the full-length catla IL-10 (cIL-10) gene had five exons and four i...
Article
Genetic information linked to the testicular development of Labeo rohita (rohu) could contribute towards understanding and improving breeding efficiency. A rohu testis-enriched cDNA library was generated by the subtractive and suppressive hybridization technique to identify differentially expressed genes. Two-hundred and forty differentially screen...
Article
Selection for disease resistance in fish may be performed directly on basis of survival data obtained in controlled challenge trials, or indirectly using information from immunological or molecular markers linked to differential survival. In the present study, several key innate immune parameters were measured in aeromoniasis resistant and suscepti...
Article
The innate immune response in fish represents an early and rapid defense against pathogens. The present study aims at looking into ontogeny of innate immune system in the teleost, Labeo rohita using RT-PCR based approach. Total RNA extracted from unfertilized and fertilized eggs, and hatchlings (hatched at 28 ± 2 °C) at 0, 1, 3, 6, 12, 24 h, and 3,...
Article
Study was conducted to find out the comparative susceptibility of fingerlings of seven species of carps (Labeo fimbriatus, L. rohita, L. calbasu, Catla catla, Ctenopharyngodon idella, Cyprinus carpio and Hypophthamichthys molitrix) grown under both mono and polyculture to Lernaea cyprinacea infection. Under monoculture, C. carpio, L. rohita and L....
Article
Full-text available
We report here for the first time significant advancement of sexual maturation and off-season breeding of Indian major carp (IMC) species, rohu (Labeo rohita), catla (Catla catla) and mrigal (Cirrihinus mrigala) through photothermal manipulation during winter at least 4-5 months prior to their normal spawning season (June-August). The spent fishes...
Article
Embryonic stem (ES)-like cells were derived from mid-blastula stage embryos of a freshwater fish, catla Catla catla, under feeder-free condition and designated as CCES cells. The conditioned media was optimized with 10% foetal bovine serum (FBS), fish embryo extract (FEE) having 100 µg ml(-1) protein concentration, 15 ng ml(-1) basic fibroblast gro...
Article
The worldwide use of Nile tilapia (Oreochromis niloticus Linnaeus, 1758) in aquaculture represents a somewhat unique scenario. The natural distributions and global genetic resources of tilapias are in Africa, yet the main centers of utilization for aquaculture are primarily in Asia. Within a few decades, Nile tilapia graduated from being an ‘orphan...
Article
Among three tilapiine species, Oreochromis aureus (Steindachner), O mossambicus (Peters) and O. niloticus (L.), fifteen banding positions of haemoglobin were resolved using citric acid-aminopropylmorpholine pH 7.0 buffer. O. mossambicus and O. aureus displayed 7 and 8 fixed band locations, respectively, whereas eight strains of O. niloticus from As...
Article
The present study is based on data recorded from fish of the third generation of the GIFT project (Genetic Improvement of Farmed Tilapias). The objective of the study was to compose a synthetic base population of Nile tilapia for further selective breeding, and to estimate phenotypic and genetic parameters in that population. The base population wa...
Article
With the recent availability of genetically improved Nile tilapia strain in the Philippines, it is important to look at the existing profile of tilapia hatchery operations in the country to help examine the potential strategies in the dissemination of the improved strain among hatchery operators. This paper attempts to analyze the socioeconomics an...
Article
The International Center for Living Aquatic Resources Management (ICLARM), in collaboration with its partner organizations, has developed an improved strain of Nile tilapia (known as the GIFT strain) which shows superior performance in terms of growth and survival in on‐station conditions. The on‐station performance of the GIFT strain may, however,...
Article
Twenty tagged individuals were randomly sampled from each of 42 full-sib families within 21 randomly chosen half-sib families of Nile tilapia (Oreochromis niloticus) among the families representing the third generation of selection for improved growth performance in the GIFT project, and communally stocked in an earthen pond. The occurrence of sexu...
Article
A study was carried out on lysozyme activity and spontaneous haemolytic (SH) activity of blood serum from 388 individuals of Nile tilapia (Oreochromis niloticus) coming from 42 full-sib groups within 21 paternal half-sib groups. The lysozyme activity was measured at both 15°C and 30°C incubation temperatures, whereas SH activity was measured at 30°...
Article
A complete diallel cross experiment was carried out with eight strains of Nile tilapia (Oreochromis niloticus). The strains represented four wild populations collected from various locations in Africa and four populations that had been reproduced over a large number of generations for tilapia farming in Asia. Growth performance was recorded in a to...
Conference Paper
Full-text available
The focus of this paper is on the results of a multidisciplinary research and development program implemented by ICLARM and its partners since 1988 under the auspices of two projects: the “Genetic Improvement of Farmed Tilapia” (GIFT) and the “Dissemination and Evaluation of Genetically Improved Tilapia in Asia” (DEGITA). The program has two themes...
Conference Paper
Full-text available
The focus of this paper is on the results of a multidisciplinary research and development program implemented by ICLARM and its partners since 1988 under the auspices of two projects: the “Genetic Improvement of Farmed Tilapia” (GIFT) and the “Dissemination and Evaluation of Genetically Improved Tilapia in Asia” (DEGITA). The program has two themes...
Article
Full-text available
A study to characterize the eight strains of Oreochromis niloticus (Egypt, Ghana, Senegal, Kenya, Israel, Singapore, Taiwan and Thailand) was conducted using truss morphometrics. To remove the effects of size, a standard body size for each sex was established. Principal Component Analysis (PCA) was used to explore intraspecific variation by plottin...
Chapter
This chapter discusses the Nile tilapia genetic resources along the lines of documentation, characterization, evaluation, utilisation, and conservation. Tilapias possess remarkable attributes for aquaculture: excellent growth rates on low protein diets whether cropping natural aquatic production or receiving supplementary feeds; tolerance of wide r...
Article
Four African wild strains (Egypt, Ghana, Senegal and Kenya) and four established Asian farmed strains of Nile tilapia, Oreochromis niloticus (popularly known in the Philippines as ‘Taiwan’, ‘Thailand’, ‘Singapore’ and ‘Israel’) were analysed electrophoretically at 30 protein loci to estimate genetic differences among the strains. All strains shared...
Article
Growth curves of 1370 individually tagged fingerlings (initial body weight 1.8–14.6 g; age 78–119 days) of seven strains of Oreochromis niloticus: Egypt, Ghana, Senegal, ‘Israel’, ‘Singapore’, ‘Taiwan’ and ‘Thailand’, reared communally in fertilized earthen ponds, were followed by sampling every 3 weeks during a 90-day production cycle. Predictabil...
Article
Growth and reproduction traits were observed in seven strains of Oreochromis niloticus from Egypt, Ghana, and Senegal (bred from wild collected stocks recently imported to the Philippines from Africa), and ‘Israel’, ‘Singapore’, ‘Taiwan’ and ‘Thailand’ (strains maintained for aquaculture purposes in the Philippines), respectively. Twenty individual...
Article
Full-text available
Results from two consecutive generations of testing to evaluate the growth performance of eight different strains of Nile tilapia (Oreochromis niloticus) in eleven different farm environments are reported. The eight strains include four new strains recently imported to the Philippines from Egypt, Ghana, Kenya and Senegal; and four established Asian...
Article
Full-text available
Results from two consecutive generations of testing to evaluate the growth performance of eight different strains of Nile tilapia (Oreochromis niloticus) in eleven different farm environments are reported. The eight strains include four new strains recently imported to the Philippines from Egypt, Ghana, Kenya and Senegal; and four established Asian...
Article
Full-text available
Biologists have long employed morphometry as a tool in identification, grouping of populations and stock identification of fish. For tilapias, studies have been made on the variability of morphomeric characters in Egyptian species (Libosvarsky and Bishara 1986). Truss morphometrics, where the body shape is divided into a series of 'cells' each of w...
Article
Induced spawning of Indian major carps by hypophysation has been practiced extensively since the mid-nineteen-fifties. At present, all hatcheries function as isolated genetically closed units raising their own stock of breeders and producing fish seed for distribution to grow-out areas. The individuals for replenishment of broodstock are those whic...
Article
Artificial selection for genetic improvement is not carried on in Indian fish hatcheries. However, the process of selection of potential breeders and artificial propagation involves indirect selection, the nature of which will be determined by the type of breeders selected for breeding.The present study is focused on females belonging to two carp s...
Article
The present study on the carp species Labeo rohita in Karnataka State, south India, shows that it is possible to obtain quantitative estimates of pre-reproductive growth rate, age at first spawning and present age by appropriate interpretation of scale features such as the number of growth checks, circuli counts and spacing between circuli. The uno...

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