Amanda Foss

Amanda Foss
Greenwater Laboratories · Chemistry Laboratory

M.S.

About

25
Publications
8,410
Reads
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566
Citations
Introduction
Currently I am researching microcystin metabolism and applying analytical techniques for the diagnosis of intoxication. Developing analytical approaches for researching and monitoring other cyanotoxins keeps the wheels turning.
Additional affiliations
January 2012 - present
July 2006 - present
Greenwater Laboratories
Greenwater Laboratories
Position
  • Chemist
July 2006 - June 2016
Greenwater Laboratories
Greenwater Laboratories
Position
  • Chemist
Education
September 2012 - May 2014
University of Florida
Field of study
  • Forensic Toxicology
September 2008 - May 2012
University of Florida
Field of study
  • Fisheries & Aquatic Science
September 2001 - April 2006
Lake Superior State University
Field of study
  • Chemistry

Publications

Publications (25)
Article
Full-text available
Microcystins (MCs) and nodularins (NODs) exhibit high structural variability, including modifications of the Adda (3S-amino-9S-methoxy-2S,6,8S-trimethyl-10-phenyldeca-4E,6E-dienoic acid) moiety. Variations include 9-O-desmethylAdda (DMAdda) and 9-O-acetylDMAdda (ADMAdda) which, unless targeted, may go undetected. Therefore, reference standards were...
Article
Microcystins have been detected in raw and finished drinking water using a variety of techniques, including assays (immunoassay, phosphatase inhibition) and HPLC (UV, MS/(MS)). The principal challenge to microcystin analysis is accounting for the over 150 variants that have been described. A confirmatory individual variant HPLC analysis is prone to...
Article
In 2013 and 2015, the Pennsylvania Department of Environmental Protection conducted a survey of lotic habitats within the Susquehanna, Delaware, and Ohio River basins in Pennsylvania, USA, to screen for microcystins/nodularins (MCs/NODs) in algae communities and smallmouth bass (Micropterus dolomieu). Periphyton (68 from 41 sites), juvenile whole f...
Article
β-Methylamino-L-alanine (BMAA) has been identified as the potential cause of the amyotrophic lateral sclerosis/parkinsonism-dementia complex (ALS/PDC) observed in the Chamorro people of Guam. The principal hypothesis for BMAA exposure and intoxication relies on the biomagnification of BMAA in flying fox specimens ingested by the Chamorro people. Al...
Article
Microcystins (MCs) are hepatotoxic and potentially carcinogenic cyanotoxins. They exhibit high structural variability, with nearly 250 variants described to date. This variability can result in incomplete detection of MC variants during lake surveys due to the frequent use of targeted analytical methods and a lack of standards available for identif...
Article
Several genomes of Nostocales ADA clade members from the US Pacific Northwest were recently sequenced. Biosynthetic genes for microcystin, cylindrospermopsin or anatoxin-a were present in 7 of the 15 Dolichospermum/Anabaena strains and none of the 5 Aphanizomenon flos-aquae (AFA) strains. Toxin analyses (ELISA and LC-MS/MS) were conducted to quanti...
Article
Two cyanobacterial strains, one collected from an epidermal mat present on a dead bottlenose dolphin and the other as a bioaerosol 457 m (1500 ft) above the river, were recently analysed from the St. Johns River, Jacksonville, Florida, USA. Both samples had major phenotypic plasticity which confused morphological identification. Amplicon sequencing...
Article
Full-text available
In the summer of 2018, six dogs exposed to a harmful algal bloom (HAB) of Microcystis in Martin County Florida (USA) developed clinicopathological signs of microcystin (MC) intoxication (i.e., acute vomiting, diarrhea, severe thrombocytopenia, elevated alanine aminotransferase, hemorrhage). Successful supportive veterinary care was provided and led...
Technical Report
Full-text available
Some Known and Suspected Toxigenic Cyanobacteria from Around the World This list includes taxa that have had toxins identified, have been implicated in toxic events in the field, or have elicited a positive response in laboratory assays. New toxigenic cyanobacteria species are continually being discovered and data concerning known and suspected tox...
Article
In the summer of 2012, over 750 dead and dying birds were observed at the Paul S. Sarbanes Ecosystem Restoration Project at Poplar Island, Maryland, USA (Chesapeake Bay). Clinical signs suggested avian botulism, but an ongoing dense Microcystis bloom was present in an impoundment on the island. Enzyme-linked immunosorbent assay (ELISA) analysis of...
Poster
Microcystins (MCs) are a group of hepatotoxic heptapeptides that inhibit protein phosphatases, and are produced by many species of cyanobacteria. Most MCs contain the unusual β-amino acid “Adda5 ”, γ-linked D-Glu6, and N-methyldehydroalanine (Mdha 7) at positions 5–7, with more than 250 different microcystin congeners having been reported in the li...
Article
In 2016, the Pennsylvania Department of Environmental Protection conducted a limited survey of streams in the Susquehanna River basin in Pennsylvania, USA, to screen for microcystins/nodularins and anatoxin-a (ATX) and homoanatoxin-a (HTX). Testing revealed the presence of HTX in samples collected from the Pine Creek basin, with ATX present at lowe...
Article
Microcystins/Nodularins (MCs/NODs) are potent hepatotoxic cyanotoxins produced by harmful algal blooms (HABs) that occur frequently in the upper basin of the St. Johns River (SJR), Jacksonville, FL, USA. Areas downstream of bloom locations provide critical habitat for an estuarine population of bottlenose dolphins (Tursiops truncatus). Since 2010,...
Article
Harmful Algal Bloom species are ubiquitous and their blooms occur in the Arabian Gulf. In this study, two cruises were performed in 2012 and 2013 to collect phytoplankton samples from 4 sites in the Arabian Gulf. Toxin analyses of phytoplankton samples for 32 algal toxins from 5 different toxin groups were conducted on the samples using both enzyme...
Article
Full-text available
Paralytic Shellfish Toxins (PSTs) are highly toxic metabolic by-products of cyanobacteria and dinoflagellates. The filamentous cyanobacterium Lyngbya wollei produces a unique set of PSTs, including L. wollei toxins (LWT) 1-6. The accurate identification and quantification of PSTs from Lyngbya filaments is challenging, but critical for understanding...
Article
Full-text available
Microcystin poisoning was diagnosed in a dog exposed to a Microcystis aeruginosa-dominated, freshwater, harmful algal bloom at Milford Lake, Kansas, which occurred during the summer of 2011. Lake water microcystin concentrations were determined at intervals during the summer, using competitive enzyme-linked immunosorbent assays, and indicated extre...
Chapter
Full-text available
The eutrophication of water resources, mainly attributed to antrophogenic activities such as sewage and agricultural runoffs, has led to a worldwide increase in the formation of cyanobacterial harmful algal blooms (Cyano-HABs). Cyano-HABs have the ability to produce and release toxic compounds, commonly known as cyanotoxins, which comprise a potent...
Article
Full-text available
We conducted a study of recreational exposure to microcystins among 81 children and adults planning recreational activities on either of three California reservoirs, two with significant, ongoing blooms of toxin-producing cyanobacteria, including Microcystis aeruginosa (Bloom Lakes), and one without a toxin-producing algal bloom (Control Lake). We...

Questions

Question (1)
Question
I would like to calibrate our LC-MS/MS system for anatoxin-a(s) analysis and cannot seem to find any analytical standards available.

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