Amal D Premarathna

Amal D Premarathna
Tallinn University | TLU · Institute of Mathematics and Natural Sciences

B.Sc | M.Phil
Polysaccharide based biomaterials from marine sources: Synergistic combinations between seaweed derived biomolecules.

About

52
Publications
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Introduction
My principal research interests include a wide range of topics related to the fields of medicinal properties; Polysaccharide based biomaterials from seaweeds. I am particularly interested in understanding the wound healing of seaweeds, cell culture experimentation and animal models. These diverse fields have given me many opportunities to improve scientific knowledge and work on many research studies.
Additional affiliations
March 2016 - December 2018
University of Peradeniya
Position
  • MPhil
Description
  • M.Phil. study investigated medicinal properties of seaweeds, as well as documenting; Preclinical evaluation of Sargassum ilicifolium (seaweed) extracts using in vivo and in vitro wound-repair model demonstrated enhanced wound-healing activities. Voucher specimens were collected and cells/tissues were collected for molecular investigations and for a study looking at immunomodulatory effects of seaweeds.
Education
April 2011 - February 2015
Ocean University of Sri Lanka
Field of study
  • Fisheries and Marine Science

Publications

Publications (52)
Article
A porphyran degrading enzyme was obtained from the marine bacterial strain Zobellia uliginosa with a secondary funoran degradation activity. The optimal fermentation conditions were determined using one-factor-at-a-time experiments and the bacteria produced highest amount of enzyme after 72 h of incubation at 25 °C. The optimal fermentation medium...
Article
Full-text available
Abstract Human envenoming from the bite of the abundant hump-nosed pit viper (Hypnale spp.) (HNPV) is a frequent occurrence with victims experiencing unpleasant and sometimes life-threatening consequences. Further, clinico-pathology, treatment and management measures in HNPV envenomed dogs are under recognized. Prospective investigations were perfo...
Article
Full-text available
The discovery of antibiotics becomes one of the maximum groundbreaking improvements of drugs. This healthcare staple, liable for treating illnesses that when had been incurable, is now turning into much less and much less powerful due to antibiotic resistance. The microorganism accountable can emerge as immune to this medicinal drug through genetic...
Article
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Nyctaginaceae, the Four O'Clock Family, consists of around 33 genera and 290 species and it is well recognized for its ornamental and medicinal values. Pisonia alba span is one such medicinal plant of the Nyctaginaceae family with excessive medicinal practicable and is freely reachable in India. This study was intended to determine the major class...
Article
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Cholesterol is one of the vital compounds required maintain principal metabolic pathways and structural material in the human and animal body. Insects are lack of the essential enzymes required for the biosynthesis of cholesterol from precursor molecules in nature and which makes them depend on dietary cholesterol. Thus the blocking of cholesterol...
Article
Full-text available
A momentous evolution has been made in describing cellular hierarchy and the stem cell (SC) niche in the human mammary gland. Mammary stem and progenitor cells exist in two different states: Epithelial and mesenchymal. Several features of the mammary SCs predispose them to play a critical role in breast cancer (BC) initiation, progression, and meta...
Article
Full-text available
Seaweeds have been regarded as a reservoir of biologically active molecules that are important in the pharmaceutical industry. The aim of the present study was to explore the wound healing properties and to assess the safety of the seaweed Sargassum ilicifolium and Ulva lactuca. Enhanced cell proliferation and cell migration activities were observe...
Article
Full-text available
Background Momordica charantia or bitter melon is a well-known vegetable with a number of therapeutic actions in Ayurvedic medicine. Alpha-eleostearic acid, a conjugated trienoic fatty acid present in bitter melon is proven to have anticancer properties. Crude seed oil from local bitter melon varieties could be an effective and economical anticance...
Article
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Neurodegenerative movement disorder of the central nervous system (CNS) (Parkinson’s disease [PD]) is characterized by necrosis of dopaminergic neurons in the substantia nigra pars compacta region of the midbrain. The etiology of PD is still unknown and is believed to be multifactorial, oxidative stress and mitochondrial dysfunction are widely cons...
Article
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The average human body consists of trillions of small building blocks called cells. Within each of these cells, there are numerous genes. Genes contain the necessary genetic information to govern our bodily functions; as an example particular gene carries this unique “code” that is needed for the synthesis of this specific protein, which, in turn,...
Article
Algae are a great source of mineral salts, vitamins, proteins and trace metals necessary for human metabolism. Interest of introducing algae into human nutrition in western countries has increased in recent years. Humans can also consume algae indirectly because they are a source of different compounds used in the food industry such as agar, algina...
Article
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Globally, approximately 70% of deaths from cancer occur in low- and middle-income countries due to the five leading behavioral and dietary risks such are high body mass index, intakes of low fruit and vegetable lack of physical activities, tobacco, and alcoholism. In low and middle-income countries, about 25% of infectious diseases are hepatitis an...
Article
Full-text available
Abstract: There is limited information on clinical profiles, treatment, and management aspects of Indian cobra (Naja naja) bite envenoming in dogs in Sri Lanka. Dogs with cobra bites presented to the Veterinary Teaching Hospital (VTH), University of Peradeniya, were prospectively studied over a period of 72 months; local and systemic clinical manif...
Article
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Worldwide statistics show8-12% of married couples have infertility problems. Between 80-90% of these were reported due to the male, and 10% due to the female. Orthodox western treatments are expensive and have side effects, so we tried an Ayurvedic regime, with its lower costs and fewer side effects. Fifteen infertile couples were selected for the...
Article
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Elimination of malaria has been successfully recorded, but still continues as a major health hazard in developing countries. As malaria control relies mainly on indoor residual spraying of insecticides, it has become a fear task due to widespread resistance in malaria vectors. However, any disease control studies taken can influence its success (ve...
Article
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Background Seaweeds are an important source of bioactive compounds which are applied in various aspects of medicinal investigations. The present study was conducted to investigate cytoxicity (in-vitro and in-vivo) and wound healing activity of different seaweed species in Sri Lanka. Methods Twenty-three seaweed samples, belonging to Phaeophyta (Br...
Article
Full-text available
Seaweeds are aquatic angiosperms, which are widespread in many coastal settings. Due to their various type of application, for instance, therapeutic agents, they are considered as one of the key environmental resources. In order to find out the diversity, abundance and pattern of species distribution of algae, a study was carried out in the three s...
Preprint
Full-text available
Objective Fish and crustacean are important sources of animal proteins and other essential nutrients required in the human diet. They are widely accepted for their high palatability, high protein content and tender flesh. In this study, proximate analyses were done for flesh of brackish water shrimp species Penaeus monodon and fresh water fish spec...
Article
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Introduction: Russell's viper envenoming in dogs is a significant problem in Sri Lanka. The current study focused on investigating clinical profile, laboratory findings of three selected tests and to develop a treatment strategy with Indian polyvalent Anti-Venom Serum (AVS). It was also intended to report adverse effects and complications caused b...
Article
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Seaweed is an enormous resource comprised with natural bioactive compounds with several therapeutic effects including anticancer activity. In this context, the biochemical composition of seaweed plays a major role. Many biochemical compounds isolated from seaweed, fractions of seaweed and crude extracts has revealed ability of seaweed to fight agai...
Article
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Objective: Glutathione is an antioxidant, which presents in mammalian and known as the most powerful antioxidant. Glutathione is called as "Master Antioxidant" because of its intracellular and possesses the aptitude to exploit the performance of other antioxidants, these include vitamins C & E, CoQ10 (ubiquinone) and alpha-lipoic acid, and rich in...
Article
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Macroscopic and microscopic evaluation and chemical profiling techniques have their own limitations for authentication of plant species. DNA-based markers give the new directions to pharmacognosy. It is important to distinguish accurately the authentic plant sources for herbal drugs because adulteration and substitutions diminish their efficacy and...
Article
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Coral reefs being highly diverse to marine ecology, yet most of the mystery remain unsolved. Coral reefs are of ecological as well as economic importance. Corals support commercial and subsistence fisheries, tourism, and are of medicinal value. It also creates a barrier against storms, flooding, and erosion by reducing wave action. Further, Corals...
Article
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Background: Seaweeds contain biologically active molecules which are important in the pharmaceutical industry. The present study was aimed to investigate the effects of aqueous extract of seaweeds: Sargassum illicifolium (S.i) on the stimulation of mouse fibroblast cell proliferation and migration. Materials and methods: Sargassum illicifolium-SW1...
Article
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Hygrophila auriculata is belonging to family Acanthaceae, Usually called as "Neermulli in Tamil" is a grow in wet places. It is an important herbal plant, broadly spread in Sri Lanka India, and is used to treat various diseases. Hygrophila auriculata (L.) Nees, Acanthaceae, is a source of traditional medicine; the aerial parts of the plant are used...
Article
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Coagulopathy is an important, common systemic clinical syndrome caused by snake envenoming in the world. The reference ranges, so far, used in the Veterinary Teaching Hospital (VTH) for clinical evaluation of dogs with coagulation abnormalities were from western countries which not the ideal is considering the geographical differences. Therefore, i...
Article
Full-text available
Seagrasses are aquatic angiosperms located in shallow marine and brackish water environments and considered as a key environmental resource. Seagrasses are not only important as primary producers in aquatic environment but also they are important to protect coastal environment from erosion and provide environment to large number of species. This st...
Article
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The immune system is known to be involved in the etiology as well as pathophysiological mechanisms of many diseases Traditional medicine gives emphasis on promotion of health a concept of strengthening host defenses against different diseases. Rasayana plants are particularly recommended for the treatment of immune disorder, Ayurveda may play an im...
Poster
Full-text available
Seaweeds are aquatic angiosperms widespread in coastal environments and considered as a key environmental resource because of numerous importance of therapeutic agent. In this study extensive surveys carried out the algal diversity on their abundance and distribution were determined along transects down to the bottom sand plain on Ahagama, Thalpe a...
Article
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This report describes the results of clinical, cytological and histopathological findings associated with subcutaneous extraskeletal osteosarcoma of a 05 years old Australian Silky Terrier. The tumor was located on the head above the frontal and parietal part of the skull. A definitive diagnosis was based on histological examination of the mass. Ev...
Article
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Dogs (Canis familiaris) have several behavioral characteristics that may contribute to snakebites. Dogs generally are inquisitive, lack ophidiophobia (fear of snakes), and may attack a snake in defense of self or a human companion. This is a descriptive cross-sectional study of 115 dogs presented to the Veterinary Teaching Hospital during a 36 mont...
Article
Full-text available
This study was carried out in a commercial broiler farm with 5000 birds. High mortality (27.3%) among 5 days old chicks was reported in a broiler farms at central province in Sri Lanka on March 2014. Among 5000 birds, 3000 were affected and more than 500 birds were dead at the time of complaint. Affected birds showed signs of respiratory difficulti...
Poster
Full-text available
Previous studies have proven that Seaweeds contain bioactive molecules with therapeutic values. However, seaweed properties in cutaneous wound healing have not explained yet. Therefore, a study was conducted to explore the potential wound healing properties of a seaweed (Sargassum ilicifolium) extracts using a an experimental rabbit model. In this...
Presentation
Full-text available
seaweeds have biological molecules which are important in pharmaceutical industry. But, in Sri Lanka the biomedical potentials of seaweeds have not yet received much attention. The present study was conducted to explore the potential wound healing properties and investigate the safety assessment. For this study seaweed samples were collected from t...
Conference Paper
Full-text available
Seaweeds have been shown to be the sources of biologically active natural compounds with pharmacological potentialities. But, in Sri Lanka the biomedical potentials of seaweeds have not yet received much attention. The present study was conducted to explore the potential wound healing properties of selected seaweeds using a mice model. For this stu...
Poster
Full-text available
Seaweeds have been shown to be the sources of biologically active natural compounds with pharmacological potentialities. But, in Sri Lanka the biomedical potentials of seaweeds have not yet received much attention. The present study was conducted to explore the potential wound healing properties of selected seaweeds using a mice model. In this stud...
Article
Full-text available
The effects of clove oil, xylazine, cooling, carbon dioxide (CO2), and magnesium sulfate (MgSO4) were evaluated separately, in normal, mature crabs (n = 55) of Portunus sanguinolentus having an average weight of 69.98 ± 3.56 g (mean ± SE). In general, crabs may be anesthetized by exposure to the gaseous anesthetic agent, by immersion in a solution...
Conference Paper
Full-text available
Seaweeds have been shown to contain active natural compounds with numerous pharmacological potentialities. But, in Sri Lanka the biomedical potentials of seaweeds have not received much attention. The present study was conducted to explore the potential activity of selected seaweeds in wound healing using a mice model. Wound healing is a general re...
Conference Paper
Full-text available
Seafood lipids are rich in polyunsaturated fatty acids such as EPA and DHA. The analysis of fatty acids has become increasingly important as human nutritional and health implications. In this study, meat of male crab was analyzed for fatty acid composition and extracted oil from crab meat. Crab oil samples (n=3) and fish oil samples (n=3) were also...
Article
Full-text available
The meat of male P.pelagicus(n=3) was analyzed for protein, fat, moisture and ash contents by proximate analysis. The flesh of shark (Caracharhinus spp.)(n= 3),thalapath (Istiophorus spp.)(n= 3) were also analyzed for water-soluble protein content. Peptide-mapping was also carried out for the water-soluble protein fraction of all three types of sam...
Conference Paper
Full-text available
Size-frequency distributions of coral colonies reveal important characteristics of composition of coral populations by providing insight into past events and have some predictive power with respect to population development on the reef. There are several factors affecting the size, frequency distribution of corals. The aims of this study were to 1)...
Poster
Full-text available
The authors have previously studied the effects of clove-oil (eugenol), xylazine and magnesium sulphate (MgSO4) on the same species of crabs. The present study was carried out to evaluate the effects of carbon dioxide (CO2) and to further study the effects of clove oil on blood-spotted crab (Portunus sanguinolentus), as anaesthetic agents. The effe...
Conference Paper
Full-text available
Live export market, nature photography and laboratory research have increased the interest on anaesthesia of crustaceans. Currently, the attention on anaethesia of crustaceans is unsatisfactory and therefore, this study was carried out with the objective of evaluating the effects of three anaesthetic agents on blood-spotted crab (Portunus sanguinol...

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Projects

Projects (9)
Project
The treatments available for wound healing include are highly expensive and have side effects. Developing and develop countries have to spend a large sum of money in importing drugs and machines used in wound healing treatment. Finding alternate, effective and less expensive methods for treatments of wound healing will bring immense benefits.
Project
Focuses on development of novel polysaccharide-base biomaterials with beneficial rheological and biological properties (Can be used as high-value ingredient in food, pharmaceutical or cosmetic formulations)
Project
The main goal of this study will be the determination of funoran degradation ability of enzymes from the β-agarase and porphyranases family and present a comparative study of enzyme activity based on the amount of produced oligosaccharides.