A.M. Sahilah

A.M. Sahilah
Universiti Kebangsaan Malaysia | ukm · Department of Food Science

BSc (Microbiology)(Hons.); MSc (Biotechnology); MSc (Microbiology); PhD (Molecular Biology)

About

87
Publications
27,248
Reads
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923
Citations
Citations since 2016
33 Research Items
686 Citations
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
Introduction

Publications

Publications (87)
Article
Full-text available
Cultured meat is meat produced from stem cell biopsies of cattle. Stem cells were cultured in a bioreactor in the presence of serum to grow the flesh to maturity. Cultured meat technology originated from regenerative medical technology; however, it has been given a new lease of life to produce cultured meat as an innovative food source in the futur...
Article
Full-text available
Meat culturing technology goes beyond laboratory research and materialises in the market. Nonetheless, this technology has raised concerns among Muslim consumers worldwide due to its medium, especially foetal bovine serum (FBS), which originates from blood. Thus, the aim of this research was to determine the halal status of cultured meat by detecti...
Article
Full-text available
Lactic acid has become one of the most important chemical substances used in various sectors. Its global market demand has significantly increased in recent years, with a CAGR of 18.7% from 2019 to 2025. Fermentation has been considered the preferred method for producing high-purity lactic acid in the industry over chemical synthesis. However, the...
Article
Kajian ini dijalankan untuk mengesan asid deoksiribonukleik (DNA) spesies haiwan dalam campuran minyak sapi menggunakan teknik tindak balas rantaian polimerase (PCR) multipleks. Kebolehdapatan DNA daripada produk berminyak membolehkan analisis PCR dijalankan dan sekaligus boleh menentukan kehadiran spesies haiwan produk tersebut dengan menggunakan...
Article
Selulosa bakteria (SB) adalah biopolimer yang penting kepada industri makanan kerana ciri uniknya seperti kapasiti pegangan air (WHC) dan kekuatan mekanikal yang tinggi. SB berpotensi untuk diinovasi dengan penambahan bahan pengawet bagi menyelesaikan masalah pencemaran makanan oleh bakteria patogen. Strain Komagataeibacter xylinus mampu menghasilk...
Article
Full-text available
A total of 24 strains of Vibrio alginolyticus were isolated from cockles (Anadara granosa) and identified for VibA and gyrB genes. All V. alginolyticus isolates were then tested against nine different antibiotics. In this study, the highest percentage of antibiotic resistance was obtained against penicillin (37.50%), followed by ampicillin, vancomy...
Article
In order to invent a porcine gelatine detection device using microbial resources, bacterial enzymes with a preference towards porcine gelatine and their candidate genes were evaluated. Five (n=5) bacterial strains isolated from hot spring water and wet clay, Malaysia were screened for their gelatinase activity. The gelatinase enzyme was extracted a...
Article
Full-text available
This study was conducted to detect the presence of chicken and porcine DNA in meatballs using mitochondria DNA (mtDNA) of cytochrome b (cyt b) and nuclear DNA (nDNA) short interspersed nuclear element (SINE) species-specific primers, respectively. While, the mtDNA primers targeted transfer RNA-ATP8 (tRNA-ATP8) gene was used for 1 and 5 % (w/w) chic...
Article
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Islam is a religion that inspires its followers to seek knowledge continually and nurtures innovation, within the realms of Islamic rulings, towards an ameliorated quality of life. Up-to-date biotechnological techniques, specifically animal cloning, are involved in advancing society’s health, social, and economic domains. The goal of animal cloning...
Article
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Disposal of animal blood from slaughterhouses leads to high risk of water and land resources problem. Due of this concern, food scientists separated the plasma component and transformed into food additives that comprised with tremendous functional properties such as gelling agent, replacing fat and eggs function, emulsifier, binder agent and also h...
Article
Full-text available
Bacillus cereus is a ubiquitous foodborne pathogen, can cause food poisoning, leading to infections, have two major types of food poisoning emetic and diarrheal. Foods rich in protein such as meat are associated with foodborne outbreaks of diarrhea caused by B. cereus. The aim of this study is to isolate and identify B. cereus from ready to eat (RT...
Article
Full-text available
Bacillus cereus is a ubiquitous foodborne pathogen, can cause food poisoning, leading to infections, have two major types of food poisoning emetic and diarrheal. Foods rich in protein such as meat are associated with foodborne outbreaks of diarrhea caused by B. cereus. The aim of this study is to isolate and identify B. cereus from ready to eat (RT...
Article
Full-text available
Sixty strains (n = 60) of Vibrio vulnificus were examined for their multiple antibiotic resistance (MAR) index, plasmid profiles, and DNA polymorphisms. Thirty-seven strains (n = 37) were isolated from cockles (Anadara granosa) in Malaysia, while 23 (n = 23) isolates were isolated from clams (Mercenaria mercenaria) in Qatar. All isolates were resis...
Article
Full-text available
Enterobacter aerogenes (Strain EA1) was characterised as gelatinase species-specific bacterium to porcine and fish gelatin. This bacterium offers the possibility of enzymes production to each species gelatin. In this study, a bioinformatic analysis toward genome sequence of E. aerogenes was perfomed to determine genes encoded for gelatinase. E. aer...
Article
Full-text available
This study was conducted to determine the presence of Vibrio parahaemolyticus on 30 samples of flower crab (Portunus pelagicus) collected from Negeri Sembilan (n = 15) and Selangor (n = 15) from March to July 2014. A total of 193 isolates of Vibrio sp. were isolated from the thiosulphate citrate bile-salt (TCBS) agar medium. Of 79 Vibrio isolates w...
Article
Full-text available
This study aims to evaluate the effects of plant density on the degradation of polyaromatic hydrocarbon (PAH) compounds in soils contaminated with crude oil. In this work, Axonopus compressus was applied as a bioremediator given its extensive and successful use in phytoremediation. A. compressus was planted at the density of three (pot-3), five (po...
Article
Genomic DNA of 13 fish (n=13) species consist of four freshwater which were catfish (Clarias gariepinus), shark catfish (Pangasius larnaudii), tilapia (Oreochromis mossambicus), perch (Lates calcarifer) and nine marine species which were black pomfret (Parastromateus Niger), anchovy (Stolephorus commersonii), mabong (Rastrelliger kanagurta), red sn...
Article
This study aimed to evaluate the level of knowledge, attitude and practice (KAP) amongst food handlers in food utensils hygiene and presence of bacterial contamination on food utensils. There were two approaches used in this study. In the first approach, 134 of food handlers from 75 food premises were interviewed via questionnaires to assess their...
Conference Paper
Full-text available
Bacteria subtyping is important in epidemiological study to monitor the emergence of new strains of pathogenic bacteria and their pattern to antibiotic resistance for disease control. A total of 60 Vibrio vulnificus isolates were isolated from cockles (Anadara granosa)(37 isolates)were from Malaysia and clams (Mercenaria mercenaria) (23 isolates) w...
Conference Paper
A glasshouse experiment was conducted to study the effect of different type of compost and fertilizers on the growth of tomato (Lycopersicon esculentum). The experiment consisted of sixteen treatments. Compost of Empty fruit bunch (EFB) and cow dung is mixed in the ratio of 3:2:1 (soil: compost: sand) and put into 25.4 mm2 polyethylene bag. Organic...
Conference Paper
In this study, bioinformatic analysis towards genome sequence of E. aerogenes was done to determine gene encoded for gelatinase. Enterobacter aerogenes was isolated from hot spring water and gelatinase species-specific bacterium to porcine and fish gelatin. This bacterium offers the possibility of enzymes production which is specific to both specie...
Conference Paper
In this study, we performed bioinformatics analysis toward genome sequence of Lysinibacillussphaericus (L. sphaericus) to determine gene encoded for gelatinase. L. sphaericus was isolated from soil and gelatinase species-specific bacterium to porcine and bovine gelatin. This bacterium offers the possibility of enzymes production which is specific t...
Conference Paper
Study on the comparison of cow manure (CM) and empty fruit bunches (EFB) compost application as planting medium was conducted using four different treatments of fertilizer (without fertilizer, chemical fertilizer, organic fertilizer, and both fertilizer) on growth and yield of chili (Capsicum annum). The experiment started on August until December...
Article
Full-text available
The chitosan-stabilized ferrous sulfide nanoparticles were loaded on biochar to prepare a composite material FeS-CS-BC for effective removal of hexavalent chromium in water. BC and FeS-CS-BC were characterized by Brunauer–Emmett–Teller (BET), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (F...
Article
Full-text available
Two papaya cultivars were used to manufacture fruit leather. The objective of this study was to formulate papaya leather from locally grown papaya using natural ingredients like pectin, honey and citric acid. The fresh fruits were pureed and mix with natural ingredients, and dried in an oven at 60°C for 12 hours. The physicochemical properties and...
Article
Full-text available
Thioureas play an important role in medicinal chemistry and agricultures due to their biological activity such as antibacterial, antifungal, antiviral, herbicides, rodenticides,phenoloxidase enzymatic inhibitors, anti-HIV and anti-tumor agents. In this study, five substituted bis-thioureas have been synthesized. The isophataloyl chloride and 2,6-py...
Article
Full-text available
Bacillus cereus isolates are considered to be toxigenic and can lead to food poisoning. Cooked rice is a potentially risky food, particularly while grown in tropical countries. The present study aims to explore the prevalence of B. cereus in cooked rice from several restaurant and stalls in the area (Bangi, Kajang and UKM) in Selangor Malaysia. The...
Article
Full-text available
The aim of the present study was to determine the antimicrobial resistance pattern of Bacillus cereus isolates from fried rice samples. A total of 35 isolates of B cereus from 70 samples of fried rice were tested for their identification by 16s rDNA test and test sensitivity to fourteen commonly used antibiotics. Antibiotic resistance analysis show...
Article
Full-text available
Halal is a term that describes substances that are deemed 'pure and clean' which Muslims are allowed to consume according to Islamic law. The industrialization of food processing in the 20 th and 21 st centuries has exposed Muslims community to various ingredients such as blood plasma, transglutaminase and gelatin introduced in meatballs and surimi...
Conference Paper
In this study, we performed bioinformatic analysis toward genome sequence of L. sphaericus to determine gene encoded for gelatinase. Lysinibacillus sphaericus was isolated from soil and gelatinase species-specific bacterium to porcine and bovine gelatin. This bacterium offers the possibility of enzymes production which is specific to both species m...
Conference Paper
Study on the composting of Empty Fruit Bunch (EFB) from palm oil wastes has been conducted using two different volumes of effective microorganism (0.01% and 0.04%) in achieving ratio carbon nitrogen (C/N) below 30 within 60 days. Six windrows were prepared approximately 30 tonnes each; 2 windrows without microorganism as control; 2 windrows added w...
Conference Paper
In this study, we performed bioinformatic analysis toward genome sequence of Lysinibacillus sphaericus to determine protein translation of gelatinase. L. sphaericus was species-specific bacterium to porcine and bovine gelatin. This bacterium offers the possibility of gelatinase production. L. sphaericus was partially genome sequenced resulting in 9...
Article
Halal authentication of gelatin capsules was conducted using polymerase chain reaction (PCR)-southern hybridization on chip and conventional PCR analysis. The primers used in PCR-southern hybridization were targeted on mitochondria DNA (mtDNA) of cytochrome b (cyt b) gene and its amplicon was 276 bp. In conventional PCR, three pairs of mtDNA primer...
Article
Full-text available
Aims: The white rot fungus Pycnoporus cinnabarinus MUCL 39533 is able to reduce vanillic acid to vanillin. Reduction of vanillic acid to vanillin catalysed by the key enzyme aryl-aldehyde dehydrogenase has been reported. Here we report the isolation and cloning of aryl-aldehyde dehydrogenase from P. cinnabarinus strain MUCL 39533. Methodology and r...
Article
Full-text available
Aims: The white rot fungus Pycnoporus cinnabarinus MUCL 39533 is able to reduce vanillic acid to vanillin. Reduction of vanillic acid to vanillin catalysed by the key enzyme aryl-aldehyde dehydrogenase has been reported. Here we report the isolation and cloning of aryl-aldehyde dehydrogenase from P. cinnabarinus strain MUCL 39533. Methodology and r...
Conference Paper
Full-text available
Clove (Syzygium aromaticum) has long been used as naturopathy due to their potential antimicrobial activity against different microbial pathogens. The aim of the study was to evaluate antibacterial of stem and fruits of cloves from Iraq. Ethanol extract of cloves stem and fruits were assessed for its antibacterial activity. The antibacterial activi...
Article
Full-text available
This study was conducted to investigate the sensitivity and detection of porcine DNA in raw materials, ingredients and finished bakery products by polymerase chain reaction (PCR) - southern hybridization on chip analysis. A total of 20 samples (n=20*3) with three replicates for each samples were obtained from a bakery factory located in Bangi, Sela...
Article
Full-text available
The aim of this study has been an investigation of the presence of Bacillus cereus and detection of enterotoxigenic genes in cooked rice samples through utilizing a PCR technique. In this study the providence of B.cereus was carried out to cooked rice samples and the B.cereus isolates were investigated for enterotoxigenic gene. The cooked rice samp...
Article
Full-text available
This study reports enhanced degradation of crude oil hydrocarbons by a bacterial consortium culture (CC) immobilized onto sawdust (SD) and oil palms empty fruit bunch (OPEFB). The bacterial surface hydrophobic property of the bacterial CC was 60.3 ± 2.20 %, which suggested high bacterial cell attachment onto the carrier materials. The free bacteria...
Article
Full-text available
This study was carried out to determine the effects of fermentation on the physicochemical properties and antioxidant activities of noni extract. The analyses carried out included pH value, titratable acidity, total soluble solid (° Brix), total phenolic content (TPC) and free radical scavenging ability (DPPH) involving short (0, 1 and 2 weeks) and...
Article
Full-text available
This present was carried out to isolate and investigate antibiotic resistance pattern among Escherichia coli O157:H7 strains isolated from imported frozen meat marketed in super markets in Klang valley, Malaysia. A total of 74 samples were purchased from various supermarkets and examined for presence E. coli 0157:H7 using Fluorocult medium which sp...
Article
Full-text available
Halal surveillance on halal ingredients incorporated in surimi based products were studied using polymerase chain reaction (PCR)-southern hybridization on chip analysis. The primers used in this technique were targeted on mitochondria DNA (mtDNA) of cytochrome b (cyt b) gene sequence which able to differentiate 7 type (beef, chicken, duck, goat, b...
Conference Paper
Full-text available
Vibrio vulnificus infections are worldwide public health problems associated with illnesses resulting from consumption of raw or partially cooked seafood. The aim of this study was to investigate the presence and identification of V. vulnificus in cockles from local wet (40) and supermarkets (38) from Selangor, Malaysia from July 2013 to February...
Article
Full-text available
Biodegradation of crude oil hydrocarbon by microorganisms in seawater is generally slow because of the harsh environmental condition due to high salinity. The aim of this study was to compare sawdust (SD) and oil palm empty fruit bunch wastes as suitable carrier material to immobilise hydrocarbon-degrading bacterial consortium culture to accelerate...
Article
Full-text available
This study aim to review important virulence factors of Vibrio vulnificus that implicated in pathogen city of this bacteria, Vibrio vulnificus is a gram negative marine bacterium causing food borne infections include septicemia, gastroenteritis and wound infection in humans and marine vertebrates; as well as possess variety of virulence factors; th...
Article
Full-text available
This study was conducted to detect the presence of porcine DNA in meat products in the market using different mitochondrial (mt) DNA on conventional polymerase chain reaction (PCR). Porcine DNA detection in meat products was tested due to some issues associated with the adulteration of food products in Malaysia. This is an important issue especiall...
Article
This study was conducted to validate the capability of DNA microarray technology of OliproTM FoodPATH Gene Chip in detecting food-borne pathogens. Nine types of food-borne pathogen DNAs were used namely Bacillus cereus, Escherichia coli 0157:H7, Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella spp....
Conference Paper
Genomic DNA of Escherichia coli were characterized by enterobacterial repetitive intergenic consensus-Polymerase chain reaction (ERIC-PCR) and the presence of Shiga toxin gene-I (Stx1) and Shiga toxin gene-2 (Stx2). These isolates were originated from imported raw beef which are come from two countries namely Australia and India. The isolation of...
Conference Paper
Full-text available
This study was conducted to detect the presence of porcine DNA in meat products in the market using conventional polymerase chain reaction (PCR) and commercial PCR-southern hybridization analysis. Porcine DNA detection in meat products was tested due to some issues associated with the adulteration of food products in Malaysia. This is an important...
Conference Paper
Full-text available
Papaya (Carica papaya L. cv Eksotika) is one of the most commonly consumed tropical fruits by humans, especially Malaysians. The objective of this study was to determine the phenolic compounds and antioxidants activity in different ripening stages of papaya fruit. The fruits were harvested at five different, stages RS1, RS2, RS3, RS4, and RS5 corre...
Article
Full-text available
Genomic DNA of Vibrio parahaemolyticus were characterized by antibiotic resistance, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis. These isolates originated from 3 distantly locations of Selangor, Negeri Sembilan and Melaka (Eas...
Article
Full-text available
Papaya (Carica papaya L. cv. Eksotika) is one of the most commonly consumed tropical fruits by humans, especially Malaysians. The objective of this study was to evaluate the effect of the maturity stage (12, 14, 16, 18 and 20 weeks after anthesis) of papaya fruit on its physicochemical properties, antioxidant capacity and sensory characteristics. P...
Data
a b s t r a c t Carbohydrates from hydrolyzed biomass has been a potential feedstock for fermentative hydrogen production. In this study, oil palm empty fruit bunch (OPEFB) was treated by sulfuric acid in different concentrations at 120 C for 15 min in the autoclave. The optimal condition for pretreatment was obtained when OPEFB was hydrolyzing at...
Article
Full-text available
Coating of papaya fruit (Carica papaya L. cv Hongkong) with gum arabic (GA) has been found to enhance their shelf-life and postharvest quality. The objective of this study was to determine the effect of gum arabic on the antioxidant activity, physicochemical properties, sensory quality and microbial analysis of papaya fruits. Gum Arabic in aqueous...
Article
Full-text available
This study was conducted to validate the capability of DNA microarray technology of OliproTM FoodPATH Gene Chip in detecting food-borne pathogens. Nine types of food-borne pathogen DNAs were used namely Bacillus cereus, Escherichia coli 0157:H7, Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella spp....