Alysia Mortimer

Alysia Mortimer
Oregon State University | OSU

PhD

About

37
Publications
3,554
Reads
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633
Citations
Citations since 2016
25 Research Items
330 Citations
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
Additional affiliations
August 2015 - August 2022
Illinois State University
Position
  • Professor (Associate)
September 2013 - June 2015
University of Denver
Position
  • Professor (Assistant)
January 2013 - July 2013
Emory University
Position
  • Research Assistant Professor

Publications

Publications (37)
Article
Full-text available
Prolonged manganese exposure causes manganism, a neurodegenerative movement disorder. The identity of adaptive and non-adaptive cellular processes targeted by manganese remains mostly unexplored. Here we study mechanisms engaged by manganese in genetic cellular models known to increase susceptibility to manganese exposure, the plasma membrane manga...
Preprint
Full-text available
Prolonged manganese exposure causes manganism, a neurodegenerative movement disorder. The identity of adaptive and non-adaptive cellular processes targeted by manganese remains mostly unexplored. Here we study mechanisms engaged by manganese in genetic cellular models known to increase susceptibility to manganese exposure, the plasma membrane manga...
Article
Avian eggshell pigmentation may provide information about a female’s physiological condition, in particular her state of oxidative balance. Previously we found that female house wrens (Troglodytes aedon Vieillot, 1809) with lighter, less-maculated, and redder ground-colored shells were older and produced heavier offspring than females laying darker...
Article
Full-text available
As organisms age, they often accumulate protein aggregates that are thought to be toxic, potentially leading to age‐related diseases. This accumulation of protein aggregates is partially attributed to a failure to maintain protein homeostasis. A variety of genetic factors have been linked to longevity, but how these factors also contribute to prote...
Article
Full-text available
Significance The ability of immune cells to distinguish self tissue from nonself pathogens is a key characteristic of immunity, allowing responses to be targeted against invading pathogens while protecting against self-directed immune damage. The recognition of nonself by innate immune cells has been extensively characterized, but the mechanisms th...
Article
Full-text available
Genetic screens are used to identify genes involved in specific biological processes. An EMS mutagenesis screen in Drosophila melanogaster identified growth control phenotypes in the developing eye. One mutant line from this screen, H.3.2, was phenotypically characterized using the FLP/FRT system and genetically mapped by complementation analysis a...
Article
Full-text available
Genetic screens have been used to identify genes involved in the regulation of different biological processes. We identified growth mutants in a Flp/FRT screen using the Drosophila melanogaster eye to identify conditional regulators of cell growth and cell division. One mutant identified from this screen, B.2.16, was mapped and characterized by res...
Article
Rare genetic diseases preponderantly affect the nervous system causing neurodegeneration to neurodevelopmental disorders. This is the case for both Menkes and Wilson disease, arising from mutations in ATP7A and ATP7B, respectively. The ATP7A and ATP7B proteins localize to the Golgi and regulate copper homeostasis. We demonstrate genetic and biochem...
Article
Full-text available
As organisms are constantly exposed to the damaging effects of oxidative stress through both environmental exposure as well as internal metabolic processes, they have evolved a variety of mechanisms to cope with this stress. One such mechanism is the highly conserved p38 MAPK (p38K) pathway, which is known to be to post-translationally activated in...
Preprint
Full-text available
Rare genetic diseases preponderantly affect the nervous system with phenotypes spanning from neurodegeneration to neurodevelopmental disorders. This is the case for both Menkes and Wilson disease, arising from mutations in ATP7A and ATP7B, respectively. The ATP7A and ATP7B proteins localize to the Golgi and regulate copper homeostasis. We demonstra...
Article
Full-text available
Differences in avian eggshell pigmentation could be an honest signal of female quality that males use to inform their nestling provisioning effort. We investigated whether among-individual variation in protoporphyrin-based eggshell pigmentation in house wrens (Troglodytes aedon) reflects female fitness-associated traits and whether males use that i...
Preprint
Full-text available
As organisms are constantly exposed to the damaging effects of oxidative stress through both environmental exposure as well as internal metabolic processes, they have evolved a variety of mechanisms to cope with this stress. One such mechanism is the highly conserved p38 MAPK (p38K) pathway, which is known to be to post-translationally activated in...
Article
Copper is an essential micronutrient required for oxygen-dependent enzymes, yet excess of the metal is a toxicant. The tug-of-war between these copper activities is balanced by chaperones and membrane transporters, which control copper distribution and availability. The P-type ATPase transporters, ATP7A and ATP7B, regulate cytoplasmic copper by pum...
Preprint
Full-text available
As organisms age, they often accumulate protein aggregates that are thought to be toxic, potentially leading to age-related diseases. This accumulation of protein aggregates is partially attributed to a failure to maintain protein homeostasis. A variety of genetic factors have been linked to longevity, but how these factors also contribute to prote...
Article
Rare neurological diseases shed light onto universal neurobiological processes. However, molecular mechanisms connecting genetic defects to their disease phenotypes are elusive. Here, we obtain mechanistic information by comparing proteomes of cells from individuals with rare disorders with proteomes from their disease-free consanguineous relatives...
Article
Full-text available
During chemical transmission, the function of synaptic proteins must be coordinated to efficiently release neurotransmitter. Synaptotagmin 2, the Ca²⁺ sensor for fast, synchronized neurotransmitter release at the human neuromuscular junction, has recently been implicated in a dominantly inherited congenital myasthenic syndrome associated with a non...
Data
P[sytP-L] heterozygotes display decreased motor output compared to controls. Table providing mean sensor crossings and SEM acquired during the Drosophila Activity Monitoring assay (Fig 8). (DOCX)
Data
Molecular modeling predicts conformational changes in the C2B Ca2+-binding domain of synaptotagmin. (A) Dynamic Ramachandran Plot of the residues surrounding the P-L mutation in syt C2B. The top panels are taken from the trajectories from the P-L simulations. The bottom panels are taken from the wild type syt1 C2B simulations. Each dot in each plot...
Data
Example cross used for lethality assay in sytnull background. Potential F1 progeny are shown with the expected Mendelian distribution of each genotype, and identification of null, heterozygotes, and homozygotes at the native locus. Due to the use of the CyO balancer, ~25% of F1 progeny in control crosses (syt homozygotes at the native locus) are ex...
Data
P[sytP-L] heterozygotes exhibit decreased EJP amplitudes at most Ca2+ levels. Table providing p-values for each Ca2+ concentration tested in Fig 5. *depicts statistical significance. (DOCX)
Data
P[sytP-L] heterozygotes do not exhibit synaptic depression during and shortly after 50 Hz stimulation, but this increase in release relative to controls is not prolonged. Table providing normalized mean responses, SEM, and p-values during and after a 50 Hz stimulation train (Fig 7), where **p << 0.0001, and *p < 0.01. (DOCX)
Data
P[sytP-L] heterozygotes exhibit less synaptic depression relative to the control throughout a 10 Hz stimulation, but fail to maintain this relative increase in release upon cessation of the stimulus train. Table providing normalized mean responses, SEM, and p-values for all points tested during and after a 10 Hz stimulation train (Fig 6), where *p...
Data
Proteomic findings from ATP7A null and rescue cells. Proteome data from crosslinked ATP7A complexes isolated from human ATP7A null fibroblasts and rescue cells (ATP7AR/R) as indicated in Figure 1 and Material and methods. Cutoff selection criteria of hits are defined in Material and methods. All hits below the selection cutoff were used to curate t...
Data
Curated proteins defining the ATP7A interactome and their analysis by bioinformatics. Selected hits from BCS treated cells and copper treated cell immunoisolated ATP7A complexes. Tab with the sum of these hits (BCS+Cu Hits) was used for bioinformatics (Tabs A-C). Crapome lists hits from one of the CRAPome datasets and the proteins shared by the ATP...
Data
Reagents and proteomic findings from neuroblastoma cells. Tabs contain table of antibodies and primers used in this study. BCS and Cu tabs include all proteome data from crosslinked ATP7A complexes isolated from BCS treated and copper treated neuroblastoma cells as indicated in Figure 1 and Material and methods. Cutoff selection criteria of hits ar...
Article
Full-text available
Environmental factors and susceptible genomes interact to determine the risk of neurodevelopmental disorders. Although few genes and environmental factors have been linked, the intervening cellular and molecular mechanisms connecting a disorder susceptibility gene with environmental factors remain mostly unexplored. Here we focus on the schizophren...
Article
Full-text available
The large repertoire of circadian rhythms in diverse organisms depends on oscillating central clock genes, input pathways for entrainment, and output pathways for controlling rhythmic behaviors. Stress-activated p38 MAP Kinases (p38K), although sparsely investigated in this context, show circadian rhythmicity in mammalian brains and are considered...
Article
Full-text available
Oxidative stress remains one of the most well studied, albeit somewhat contentious, causes of age-related changes in humans. Consequently, a large number of putative antioxidant compounds are freely available in myriad formulations that are often not tested for their efficacy or regulated for quality control. Following the development of a Drosophi...
Article
Molecular mechanisms that concordantly regulate stress, life span, and aging remain incompletely understood. Here, we demonstrate that in Drosophila, a p38 MAP kinase (p38K)/Mef2/MnSOD pathway is a coregulator of stress and life span. Hence, overexpression of p38K extends life span in a MnSOD-dependent manner, whereas inhibition of p38K causes earl...
Article
Techniques to induce activity-dependent neuronal plasticity in vivo allow the underlying signaling pathways to be studied in their biological context. Here, we demonstrate activity-induced plasticity at neuromuscular synapses of Drosophila double mutant for comatose (an NSF mutant) and Kum (a SERCA mutant), and present an analysis of the underlying...
Article
Full-text available
Atonal is a Drosophila proneural protein required for the proper formation of the R8 photoreceptor cell, the founding photoreceptor cell in the developing retina. Proper expression and refinement of the Atonal protein is essential for the proper formation of the Drosophila adult eye. In vertebrates, expression of transcription factors orthologous t...
Article
Full-text available
AP-1, an immediate-early transcription factor comprising heterodimers of the Fos and Jun proteins, has been shown in several animal models, including Drosophila, to control neuronal development and plasticity. In spite of this important role, very little is known about additional proteins that regulate, cooperate with, or are downstream targets of...
Article
Drosophila DIM-7 (encoded by the moleskin gene, msk) is the orthologue of vertebrate Importin-7. Both Importin-7 and Msk/DIM-7 function as nuclear import cofactors, and have been implicated in the control of multiple signal transduction pathways, including the direct nuclear import of the activated (phosphorylated) form of MAP kinase. We performed...
Article
The Drosophila Mitogen Activated Protein Kinase (MAPK) Rolled is a key regulator of developmental signaling, relaying information from the cytoplasm into the nucleus. Cytoplasmic MEK phosphorylates MAPK (pMAPK), which then dimerizes and translocates to the nucleus where it regulates transcription factors. In cell culture, MAPK nuclear translocation...
Article
The Hedgehog and Decapentaplegic pathways have several well-characterized functions in the developing Drosophila compound eye, including initiation and progression of the morphogenetic furrow. Other functions involve control of cell cycle and cell survival as well as cell type specification. Here we have used the mosaic clone analysis of null mutat...
Article
Full-text available
Mitogen-activated protein kinases (MAPKs) phosphorylate target proteins in both the cytoplasm and nucleus, and a strong correlation exists between the subcellular localization of MAPK and resulting cellular responses. It was thought that MAPK phosphorylation was always followed by rapid nuclear translocation. However, we and others have found that...
Article
Bcl-2-associated athanogene (BAG)-family proteins are BAG domain-containing proteins that interact with the heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG-family proteins bind through the BAG domain to the ATPase domain of Hsc70/Hsp70. The BAG domain, approximately 110 amino acids in length, is a conserved region at the ca...

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