Alma Lilián Guerrero-Barrera

Autonomous University of Aguascalientes | UAA · Departamento de Morfología

Waterborne pathogens and related diseases are a major public health concern worldwide, not only by the morbidity and mortality that they cause, but by the high cost that represents their prevention and treatment. These diseases are directly related to environmental deterioration and pollution. Despite the continued efforts to maintain water safety,...

Respiratory pathogens are the main health problem in the swine industry worldwide. These pathogens are transmitted by direct contact between animals or by aerosols and however are not well known yet, if the environment works as its reservoir, inoculum and/or dispersion medium. The objective of this study was to determine the presence of respiratory...

Antimicrobial resistance is a major concern worldwide. Antibiotics are one of the most used drugs in humans and animals. The intensive use of antimicrobial agents is one of the main factors for the generation of resistant bacteria. Infections with resistant organisms and the emergence of multi-drug resistant pathogenic and non-pathogenic bacteria,...

Contamination of surface waters in developing countries is a great concern. Treated and untreated wastewaters have been discharged into rivers and streams, leading to possible waterborne infection outbreaks and may represent a significant dissemination mechanism of antibiotic resistance genes. In this study, the water quality of San Pedro River, th...

Pseudomonas aeruginosa is a leading cause of healthcare-associated infections, which are related to substantial morbidity and mortality. The incidence of Plasmid-Mediated Quinolone Resistance (PMQR) determinants has been previously reported in this bacterium. However, there is limited information regarding the presence of PMQR and carbapenemase-enc...

The persistent presence of arsenic (As) pollution in soils worldwide poses a significant threat to human and environmental health, highlighting the urgent need for effective remediation strategies. Therefore, this study aims to evaluate the capacity of the Rhizopus microsporus Os4 fungal strain, to remove As from contaminated media in laboratory st...

Background Pathogenic bacteria grow in different environments and have developed signaling systems known as two-component systems that allow them to thrive in distinct habitats efficiently. Actinobacillus pleuropneumoniae is an obligate pig pathogen that colonizes its host and survives outside it by forming biofilms. The small number of the two-com...

Synthetic colorants are widely used globally by different industries for the dyeing process. However, these chemicals pollute the environment and affect human health by causing allergies, hives, dermatitis, and cancer. This study aims to compare the effectiveness of duckweed (Lemna minor) in the removal of the Methylene Blue (MB) and Congo red (CR)...

The leaves of Psidium guajava L. are an agro-industrial by-product with an outstanding content of polyphenolic compounds; however, there are many factors which can affect the phytochemical profile when valuing this type of plant material, such as temperatures and extraction times involving in the extraction methods applied. In this context, this st...

In birds, the process of sperm maturation has not been fully described. The objective of this study was to determine in vitro parameters for the decapacitating effect of oviductal proteins on rooster spermatozoa. Aliquots with spermatozoon were incubated to in vitro induce metabolic states: capacitation, decapacitation, and the acrosomal reaction t...

Actinobacillus pleuropneumoniae (App) is a globally distributed Gram-negative bacterium that produces porcine pleuropneumonia. This highly contagious disease produces high morbidity and mortality in the swine industry. However, no effective vaccine exists to prevent it. The infection caused by App provokes characteristic lesions, such as edema, inf...

Uropathogenic Escherichia coli (UPEC) strains are among the leading causes of urinary tract infections (UTIs) worldwide. They can colonize the urinary tract and form biofilms that allow bacteria to survive and persist, causing relapses of infections and life-threatening sequelae. Here, we analyzed biofilm production, antimicrobial susceptibility, v...

The Bougainvillea glabra or bougainvillea is a climbing plant native from South America belonging to the Nyctaginaceae family. The bougainvillea is recognized worldwide for its horticultural importance, due to the color of its bracts, commonly known as “flowers,” made up of bracts, which are the striking parts, and the true flowers, which are white...

Commercial dry food (CDF) for dogs is a whole grain ration thoroughly mixed and die-cut with heat and pressure to give it the shape of kibble. CDF is formulated with several agroindustrial ingredients and by-products of agricultural and livestock origin. Contamination by Aspergillus flavus and aflatoxins (AFs) in foods has been shown to be a global...

El alimento comercial seco (ACS) para perro es una ración integral completamente mezclada y troquelada con calor y presión para darle forma de croqueta. El ACS está formulado con diversos ingredientes y subproductos agroindustriales de origen agrícola y pecuario. La contaminación por Aspergillus flavus y por aflatoxinas (AFs) en los alimentos se ha...

Carbapenem resistance (CR) is a major global health concern. CR is a growing challenge in clinical settings due to its rapid dissemination and low treatment options. The characterization of its molecular mechanisms and epidemiology are highly studied. Nevertheless, little is known about the spread of CR in food-producing animals, seafood, aquacultu...

MACÍAS, M. O.; GUERRERO, B. A. L.; VALDIVIA, F. A. G.; QUEZADA, T. T.; AVELAR, G. F. J.; SOTO, P. M. M. & DE SANTIAGO, A. S. Study of ex-ovo embryonic development of Gallus gallus domesticus. Int. J. Morphol., 41(2):668-674, 2023. SUMMARY: The domestic chicken is a species of bird that has been extensively studied in regard to its biology and as a...

Background We report large biofilm structures that covered almost the entirety of the lumen and surface of double-J stents in two postrenal transplant patients, with no development of urinary tract infection. Biofilm bacteria of one patient were integrated by coccus in a net structure, whereas overlapping cells of bacilli were present in the other...

Mexico is one of the largest guava producers in the world, so it has access to a huge amount of waste and byproducts obtained after the industrial processing of the fruit. This review discusses the potential recovery of this residue for its application as an antimicrobial agent, considering the phytochemical composition, the bioactivity reported in...

Red blood cells (RBC) morphologic evaluation through microscopy optical (OM) and SEM, provides information to forecast, evaluate, and monitor the functioning of many organs. Factors, such aging and diseases affect RBC morphology in both, human and animals. SEM is useful to evaluate RBC morphology, although its use in diagnosis and evaluation in dog...

Lead (Pb) contamination was evaluated in 24 contaminated soils and sediments samples, representative of areas affected by mining, agricultural, commercial and residential activity, during the rainy and dry season. Pseudo-total concentration in soils (15.7–527.2 mg kg−1) and sediments (16.3–4273.3 mg kg−1) was determined and protocols were developed...

Actinobacillus pleuropneumoniae is a Gram-negative bacterium and the causative agent of porcine pleuropneumonia, a highly contagious disease of pigs characterised by fibrinohaemorrhagic necrotising pneumonia. Although it has been well controlled in some developed countries, outbreaks can occur in pigs of all ages in contact with asymptomatic carrie...

Background: The incidence of urinary tract infections (UTI) in the first two months post-renal transplantation (pRT) is very high. We evaluate the efficacy of asymptomatic bacteriuria (AB) screening and treatment on the incidence of UTI in the first two months pRT METHODS: : We conducted a randomized controlled clinical trial. A urine culture was...

Background Staphylococcus aureus and Candida albicans have been co-isolated from biofilm-associated diseases such as denture stomatitis, periodontitis, and burn wound infections, as well as from medical devices. However, the polymicrobial biofilm of both microorganisms has not been fully characterized. Aims To characterize the polymicrobial biofil...

The threat of the spread of new or old infectious diseases are always a concern for human civilization. The emergence of coronavirus disease (COVID-19) and its dissemination around the world has demonstrated the risk to human health and the economy. So, it is essential to study a complete understanding of the host-pathogen interaction for the devel...

Background: Actinobacillus pleuropneumoniae (AP) is the causative agent of porcine pleuropneumonia. Apx exotoxins are the most important virulence factors associated with the induction of lesions. ApxI is highly cytotoxic on a wide range of cells. Besides the induction of necrosis and apoptosis of ApxI on porcine alveolar macrophages (PAMs), its r...

Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, an important respiratory disease for the pig industry. A. pleuropneumoniae has traditionally been considered an obligate pig pathogen. However, its presence in the environment is starting to be known. Here, we report the A. pleuropneumoniae surviving in bi...

The Baja California Peninsula has over 250 islands and islets with many endemic species. Among them, rattlesnakes are the most numerous but also one of the least studied groups. The study of island rattlesnake venom could guide us to a better understanding of evolutionary processes and the description of novel toxins. Crotalus helleri caliginis ven...

The addition of organic amendments to metal-polluted substrates reduces metal mobility, in many cases due to association with lesser mobile fractions or co-precipitation, especially in substrates with low pH and high carbonate content. However, variations in these physicochemical conditions may influence the prevailing mechanism involved in mobilit...

Introduction Microorganisms can develop into a social organization known as biofilms and these communities can be found in virtually all types of environment on earth. In biofilms, cells grow as multicellular communities held together by a self-produced extracellular matrix. Living within a biofilm allows for the emergence of specific properties fo...

Since its first patent (1897), commercial dry feed (CDF) for dogs has diversified its formulation to meet the nutritional needs of different breeds, age, or special conditions and establish a foundation for integration of these pets into urban lifestyles. The risk of aflatoxicosis in dogs has increased because the ingredients used to formulate CDF...

Actinobacillus pleuropneumonia is a swine (host) specific respiratory pathogen and the etiological agent of swine pleuropneumonia which affects pigs of all ages, many being asymptomatic carriers. This pathogen has high morbidity and mortality rates which generates large economic losses for the pig industry. Actinobacillus pleuropneumoniae is a wide...

Mesenchymal stem cells are present in adult tissues such as the human dental pulp. They are pluripotent and can differentiate into various specialized cell types in vitro through appropriate stimuli. Ameloblasts produce human tooth enamel only during embryonic development before tooth eruption, so endogenous regeneration is not possible. Various ef...

Urinary tract infections (UTIs) affect more than 150 million people, with a cost of over 3.5 billion dollars, each year. Escherichia coli is associated with 70–80% of UTIs. Uropathogenic E. coli (UPEC) has virulence factors including adhesins, siderophores, and toxins that damage host cells. Vacuolating autotransporter toxin (Vat) is a member of se...

The antibacterial effect of a hyperosmotic solution containing sorbate and ethanol on E. faecalis in planktonic state and in biofilm was evaluated. Three hyperosmotic solutions (HS-A, HS-B y HS-C) were obtained from different formulations of potassium sorbate and sodium chloride, which were tested as antimicrobials against planktonic forms of E. fa...

TagB, TagC (tandem autotransporter genes B and C), and Sha (Serine-protease hemagglutinin autotransporter) are recently described members of the SPATE (serine protease autotransporters of Enterobacteriaceae) family. These SPATEs can cause cytopathic effects on bladder cells and contribute to urinary tract infection in a mouse model. Bladder epithel...

Single extraction and sequential extraction assays were used to compare the effect of low-temperature biochar and its non-pyrolyzed composted biosolids on Pb and Cd mobility in the buffered conditions of calcareous river sediments. Results showed that amendment reduced the mobility of both metals, though under higher pH conditions, the effect of th...

Mesenchymal stem cells are present in adult tissues such as the human dental pulp. They are pluripotent and can differentiate into various specialized cell types in vitro through appropriate stimuli. Ameloblasts produce human tooth enamel only during embryonic development before tooth eruption, so endogenous regeneration is not possible. Various ef...

Mesenchymal stem cells (MSCs) are non-specialized cells that have the property of self-renewal and differ in several ways from specialized cells. They are identified in a large number of adult tissues, such as skin, adipose tissue, peripheral blood, bone marrow, pancreas, intestine, brain, hair follicles and dental tissues, being an attractive sour...

Since the existence of adult mesenchymal stem cells (MSC’s) was identified, research has been carried out to know their pluripotentiality characteristics, which allows their differentiation in various cell lineages. MSC’s are present in several tissues including skin, adipose tissue, peripheral blood, bone marrow, pancreas, intestine, brain, hair f...

Actinobacillus pleuropneumoniae, the etiological agent of porcine pleuropneumonia, represents one of the most important health problems in the swine industry worldwide and it is included in the porcine respiratory disease complex. One of the bacterial survival strategies is biofilm formation, which are bacterial communities embedded in an extracell...

bstract Background: Uropathogenic Escherichia coli (UPEC) are one of the main bacteria causing urinary tract infections (UTIs). The rates of UPEC with high resistance towards antibiotics and multidrug‐resistant bacteria have increased dramatically in recent years and could difficult the treatment. Methods: The aim of the study was to determine mult...

Worldwide, urinary tract infection (UTI) affect more than 150 million peoples per year, with an estimated cost to 3.5 billion dollars annually. The most frequent causal agent isolated is uropathogenic Escherichia coli (UPEC), which is incriminated in 70%-80% of UTI´s cases. Escherichia coli typically it´s found in gastrointestinal tract for the sev...

Concentrations of Pb and Zn, plant uptake of these metals, the influence of the plants’ growth on the physicochemical properties and metal concentrations in the tailings of an abandoned 300-year-old mine tailing dam in Zacatecas, Mexico were investigated. Tailings were found to be heavily contaminated, with average levels of 2621 ± 53 and 3827 ± 83...

Human teeth enamel, the hardest tissue in the body, is produced at embryo development during amelogenesis. Its lack of regeneration after the teeth eruption and its destruction susceptibility turn it as a good subject of study. Ameloblastin an abundant, phosphorylated, proline/glutamine-rich protein, secreted during enamel formation is a structural...

In this study, sequential column leaching coupled with batch sequential extraction assays were used to assess the fate of arsenic, chromium and copper in a wood ash contaminated alkaline grassland soil amended with biochar and its non-pyrolysed origin source material. Application of both amendments resulted in a general reduction of copper and arse...

Actinobacillus pleuropneumoniae is a Gram-negative bacterium that belongs to the family Pasteurellaceae . It is the causative agent of porcine pleuropneumonia, a highly contagious respiratory disease that is responsible for major economic losses in the global pork industry. The disease may present itself as a chronic or an acute infection character...

Due to mining being a historically important sector of the economy of Zacatecas, Mexico, substantial amounts of mining waste have been generated and inadequately disposed of near urban areas, representing a latent metal pollution problem for the population. Due to their potential use as phytostabilizers and their inherent adaptation to physicochemi...

Tooth enamel is the hardest tissue in the body. The organic matrix configuration is provided by the main proteins amelogenin, ameloblastin and enamelysin (MMP20), an enzyme that helps to shape the matrix. The aim of this study was to determine by histochemistry the expression of amelogenin and enamelysin through the rough endoplasmic reticulum in t...

The tuftelin is a secreted protein in the adamantine matrix in developing during the enamel formation. Its function continues unclarified, although it plays a role in the biomineralization of the dental organ. With the present studio the location was identified in the different structures of dental germs from human fetuses, according to the results...

Through a random sampling made in 60 pigs slaughtered in San Francisco de los Romo, Aguascalientes, Mexico, lesions characteristic of pleuropneumonia was found in 40 animals (66.6 %). From samples of lung tissue and necrotic lesions fribrin-hemorrhagic, Actinobacillus pleuropneumoniae (AP) was isolated and biochemically characterize in 55% (22 pigs...

Acetaminophen is a drug used to treat many conditions as headache, muscle aches, arthritis, backache, toothache, and fever between others, but collateral effects of this drug are not well known yet. Here is tested its effect on proximal tubule epithelium. Acetaminophen (APAP) at doses of 200, 500, 1000 and 1500 mg/Kg i.p. caused cell damage and cha...

A total of sixteen composite soil and sediment samples were collected during the rainy and dry season in Asientos, Aguascalientes, Mexico, an area recently affected by increased mining operations. Physicochemical charac- terization showed that substrates were moderately to strongly calcareous with predominantly neutral to slightly alkaline pH, mode...

Background Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, which causes important worldwide economic losses in the swine industry. Several respiratory tract infections are associated with biofilm formation, and A. pleuropneumoniae has the ability to form biofilms in vitro. Biofilms are structured commun...

The Porcine respiratory disease complex (PRDC) is a term used to describe polymicrobial respiratory infections in pigs. Respiratory diseases in pigs are common in modern pork production worldwide and are the responsible for major economic losses in the swine industry. Pathogens involved in respiratory disease in pigs vary significantly among farms,...