Allison Oakes

Allison Oakes
SUNY College of Environmental Science and Forestry | SUNY-ESF · Department of Environmental and Forest Biology

Doctor of Philosophy, Plant Science and Biotechnology

About

15
Publications
4,290
Reads
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237
Citations
Introduction
Developing elm yellows-resistant American elm lines via Agrobacterium-mediated transformation with hardwood tree pathogen-resistance genes
Additional affiliations
August 2016 - present
Onondaga Community College
Position
  • Professor (Associate)
Description
  • Taught BIO 105 "Exploring Biology" Fall 2016 Teaching BIO 121 "Introduction to Biology" Spring 2017
January 2016 - present
SUNY College of Environmental Science and Forestry
Position
  • PostDoc Position
September 2006 - April 2009
SUNY College of Environmental Science and Forestry
Position
  • Research Aide
Education
August 2009 - December 2015
SUNY College of Environmental Science and Forestry
Field of study
  • Plant Science and Biotechnology
August 2006 - May 2009

Publications

Publications (15)
Article
Full-text available
Transgenic American chestnut trees expressing a wheat gene for oxalate oxidase (OxO) can tolerate chestnut blight, but as with any new restoration material, they should be carefully evaluated before being released into the environment. Native pollinators such as bumble bees are of particular interest: Bombus impatiens use pollen for both a source o...
Article
Full-text available
Limited rooting and acclimatization success when micropropagating certain hardwood tree species may hinder conservation efforts of certain threatened and endangered species. Restoration efforts for such trees, such as the American chestnut [Castanea dentata (Marsh.) Borkh.], require a massive number of plantlets to be produced by micropropagation f...
Article
Full-text available
American chestnut (Castanea dentata (Marsh.) Borkh) was almost completely wiped out by the fungal pathogen, Cryphonectria parasitica (Murrill) M.E. Barr. Another invasive pathogen, Phytophthora cinnamomi Rands, is devastating American chestnuts in the southern region of the United States. An alternative approach for controlling these pathogens is t...
Article
Full-text available
The American chestnut (Castanea dentata) was once an integral part of eastern United States deciduous forests, with many environmental, economic, and social values. This ended with the introduction of an invasive fungal pathogen that wiped out over three billion trees. Transgenic American chestnuts expressing a gene for oxalate oxidase successfully...
Article
Full-text available
Tissue culture of plants has many applications, from producing genetically identical horticultural varieties, to production of secondary metabolites, to virus indexing, and most relevantly, developing novel traits by genetic transformation. Using Agrobacterium-mediated transformation on somatic embryos, blight-resistant American chestnuts [Castanea...
Article
Full-text available
Many hardwood tree species are being threatened by exotic pests, and for some, only genetic engineering can offer a solution before functional extinction occurs. An example of how genetic engineering can be a useful tool for forest restoration is the transgenic american chestnuts, which contain a wheat oxalate oxidase gene conferring resistance to...
Thesis
Full-text available
Developing a blight resistant American chestnut has been the goal of the American Chestnut Research and Restoration Project for decades. The development of blight resistant American chestnuts through genetic engineering and biotechnology required refining micropropagation protocols to achieve desired production of field-ready trees. The rooting an...
Article
Full-text available
The key to successful transformation of American chestnut is having the correct combination of explant tissue, selectable markers, a very robust DNA delivery system, and a reliable regeneration system. The most important components of this transformation protocol for American chestnut are the following: starting out with rapidly dividing somatic em...
Article
Full-text available
American chestnut [Castanea dentata (Marsh.) Borkh.] dominated the eastern forests of North America, serving as a keystone species both ecologically and economically until the introduction of the chestnut blight, Cryphonectria parasitica, functionally eradicated the species. Restoration efforts include genetic transformation utilizing genes such as...
Article
Full-text available
One of the most difficult processes of micropropagation is rooting and acclimatizing in vitro shoot cultures, especially for hardwood tree species. As more transgenic lines of potentially blight-resistant American chestnut (Castanea dentata) are developed, we expect to produce thousands of tiny shoots to be rooted, transferred to potting mix, and g...
Article
Full-text available
Rooting and acclimatization are two difficult stages of plant tissue culture, requiring an efficient system to reduce losses in plant materials, supplies and labor. This is especially important when working with vital transgenic cultures. Two modifications to a standard American chestnut rooting procedure, removing the activated charcoal and adding...
Article
Full-text available
American chestnut (Castanea dentata) was transformed with a wheat oxalate oxidase (oxo) gene in an effort to degrade the oxalic acid (OA) secreted by the fungus Cryphonectria parasitica, thus decreasing its virulence. Expression of OxO was examined under two promoters: a strong constitutive promoter, CaMV 35S, and a predominantly vascular promoter,...
Article
Full-text available
Softwood cuttings of American elm varieties 'Jefferson', 'New Harmony', 'Princeton', 'R18-2', 'Valley Forge', and a tissue-cultured non-transformed control clone (BP-NT) were rooted using three different treatments to determine which method would be most suitable for small-scale propagation. The treatments included aeroponic chambers, an intermitte...

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