Alisa Phulukdaree

Alisa Phulukdaree
University of Pretoria | UP · Department of Physiology

PhD - Medical Biochemistry

About

83
Publications
13,840
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,455
Citations
Additional affiliations
January 2019 - present
University of Pretoria
Position
  • Professor (Associate)
January 2013 - December 2014
University of KwaZulu-Natal
Position
  • PostDoc Position
January 2012 - December 2013
University of KwaZulu-Natal

Publications

Publications (83)
Article
Centella asiatica is commonly used in traditional medicine owing to its many therapeutic properties including but not limited to antioxidant and antitumor potential. This study examined the antioxidant and antiproliferative effects of its crude (C) and fractionated (C3) ethanolic leaf extracts in THP-1 cells. In THP-1 cells, C and C3 cytotoxicity w...
Article
Factor XIII, a transglutaminase that plays a crucial role in clot formation, consists of subunits A and B. Single nucleotide polymorphisms in Factor XIII-A have been linked to thrombotic risk. In Type 2 Diabetes mellitus (T2DM), a hypercoagulable state is thought to contribute to the high mortality rate associated with thrombotic diseases. Due to t...
Article
Patulin (PAT) is a mycotoxin produced by Penicillium and other fungi that contaminate fruit. PAT targets the kidney and is associated with nephrotoxicity. Micro-RNAs (miRNA) may offer new insights into PAT-induced nephrotoxicity. Cytochrome P450 family 1, subfamily B, polypeptide 1 (CYP1B1), involved in metabolism of dietary toxins is negatively re...
Article
Full-text available
• Statins, the standard treatment for hypercholesterolemia, among the most widely prescribed, have been associated with side effects, including statin intolerance. The aim of this study was to determine the background prevalence of SLCO1B1 SNVs in a randomly selected sample and to investigate if there are associations between SLCO1B1 SNVs and hyper...
Article
Full-text available
Patulin (PAT) is a common mycotoxin contaminant of apple products linked to impaired metabolic and kidney function. Adenosine monophosphate activated protein kinase (AMPK), abundantly expressed in the kidney, intercedes metabolic changes and renal injury. The alpha-1-adrenergic receptors (α1-AR) facilitate Epinephrine (Epi)-mediated AMPK activation...
Article
Aim: Hepatocellular carcinoma is one of the leading global epidemics. A medicinal tree, Moringa oleifera (MO), has been part of traditional treatments including cancer therapies. We investigated the apoptosis inducing effects of MO crude aqueous leaf extract (MOE) in human liver hepatocellular carcinoma (HepG2) cells. Methods: HepG2, PBMCs and Hek2...
Article
The prevalence of type 2 diabetes mellitus (T2DM) has quadrupled within three decades since 1980, affecting 422 million adults in 2016. It remains one of the most common noncommunicable chronic diseases and the underlying risk factor for cardiovascular diseases worldwide. There are different underlying mechanisms that play a role in the development...
Article
Full-text available
Background: Cancer and inflammation are associated with cachexia. Withania somnifera (W. somnifera) possesses antioxidant and anti-inflammatory potential. We investigated the potential of an aqueous extract of the root of W. somnifera (WRE) to modulate cytokines, antioxidants and apoptosis in leukaemic THP-1 cells and peripheral blood mononuclear...
Article
Azathioprine (AZA) is a well-known immunosuppressant used for many years for its ability to ensure long term disease remission in inflammatory bowel diseases (IBD) at an affordable cost to the public. However, the side effect profile has raised many concerns with numerous investigations into the risk, cause and prevention of these effects. Much of...
Article
Full-text available
The purpose of the study was to evaluate the effect nitric oxide (NOx) pollution had on maternal serum 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-OHdG) levels and neonatal outcomes in pregnant women living in Durban, South Africa (SA). Women, in their third trimester with singleton pregnancies, were recruited from the heavily industrialised south (n =...
Article
Full-text available
Interleukin (IL-)17A, plays a role in pathogenic defence, but is implicated in chronic inflammatory diseases, and has recently been associated with variable pregnancy outcomes. We investigated the role of maternal IL-17-[G197A]-specific effects of third-trimester IL-17 mRNA expression, NOx exposure levels and other variables on gestational age, in...
Article
Raw ingredients of pet food are often contaminated with mycotoxins. This is a serious health problem to pets and causes emotional and economical stress to the pet owners. The aim of this study was to determine the immunotoxicity of the most common mycotoxins (aflatoxin, fumonisin, ochratoxin A and zearalenone) by examining 20 samples of extruded dr...
Article
Fumonisin B1 (FB1) is a ubiquitous contaminant of maize that is epidemiologically linked to oesophageal cancer (OC) in South Africa. FB1-induced oxidative stress mediates toxicity in animals and human cell lines, but the effects relating to OC are limited. Given the species-specific effects of FB1, this study investigated FB1-mediated toxicity and...
Article
Full-text available
The geographical distribution of oesophageal cancer is linked to the exposure of fumonisin B1 (FB1), a mycotoxin produced by fungi that contaminates staple food worldwide. Non-genotoxic carcinogens like FB1 disturb homeostasis through increased cell proliferation or suppression of apoptosis. This study investigated the involvement of FB1 (0–20 μM)...
Article
Full-text available
Centella asiatica is a tropical medicinal plant that is commonly used in traditional medicine. Medicinal properties of C. asiatica include anti-oxidant, anti-inflammatory, and anti-cancer activity. We investigated the anti-oxidant and anti-proliferative/cytotoxic effects of a semi-purified fraction of C. asiatica ethanolic leaf extract (C3) in canc...
Article
Full-text available
Objective: Cytokines, molecules within the immune system that affect either a pro- or anti-inflammatory response, have previously been shown to influence birth outcomes. The maternal cytokine gene-environment interactions are thought to alter their expression, potentially influencing susceptibility to adverse birth outcomes. The aim of this study...
Article
Full-text available
Background: Cancer cachexia is associated with increased pro-inflammatory cytokine levels. Centella asiatica (C. asiatica) possesses antioxidant, anti-inflammatory and anti-tumour potential. We investigated the modulation of antioxidants, cytokines and cell death by C. asiatica ethanolic leaf extract (CLE) in leukaemic THP-1 cells and normal perip...
Article
The bio-synthesized DTAuNPs have an average size of 21 nm. The aggregation extent depends on the concentration of melamine, which was validated by UV-vis spectra and visual method of melamine detection was developed. The major observation in this method were the color change of DTAuNPs from red to purple due to the aggregation of ligand capped gold...
Article
Full-text available
Introduction Preeclampsia and HIV/AIDS are inflammatory conditions that contribute significantly to adverse maternal and foetal outcomes. The immune reconstitution effects of HAART on inflammatory mediators has not been adequately studied in pregnancy and may impact on the inflammatory cytokine network in women with co-morbid preeclampsia. Our stud...
Article
The aim of the present study was to investigate the effects of methonolic extracts of Tulbaghia violacea (TVL), on Jurkat cells (human T lymphocyte). Jurkat cells were treated with IC50 concentrations of TVL (leaf, rhizome and stalk) extracts as determined by MTT assay and glutathione (GSH) concentration was measured using the GSH-Glo assay. The co...
Poster
Ochratoxin A (OTA) is a nephrotoxic mycotoxin produced by Aspergillus and Penicillium fungi. It contaminates human and animal food products, and chronic exposure is associated with renal fibrosis in humans (Balkan endemic nephropathy). Resveratrol, a phytoalexin, possesses anti-cancer and antioxidant properties. We investigated the mechanism of cel...
Poster
It is estimated that 11.7 million people are on combination antiretroviral therapy (cART), with nucleoside reverse transcriptase inhibitors (NRTIs) being the most widely used anti-HIV drugs. Although cART has markedly improved the prognosis of HIV infected individuals, chronic use of cART has seen the emergence of adverse health outcomes. Several a...
Article
Full-text available
Background Sirtuin 1 (SIRT1), a class III histone deacetylase, has been identified as a candidate molecule affecting the epigenetic mechanisms of cardiovascular disease (CVD). Previous studies have shown that some SIRT1 single-nucleotide polymorphisms (SNPs) are associated with body mass index, diabetes, blood pressure, cholesterol metabolism and c...
Article
Background Petrol attendants are at risk for the development of cancers. Exposures experienced by such workers include polycyclic aromatic hydrocarbons PAH’s and the volatile organic compounds (VOCs) which are associated with DNA damage, DNA strand breaks, micronuclei and chromosomal aberrations. Aim To determine the effect of petrol exposure on D...
Article
Full-text available
Purpose: To determine the effect of petrol exposure on DNA integrity in peripheral blood lymphocytes among petrol attendants and a non-exposed comparison population. Methods: This cross-sectional study included 101 fuel station employees and 50 office-based non-exposed workers in Durban, South Africa. Participants were interviewed using a valida...
Chapter
Cancer is a leading cause of death in South Africa (SA). Nanoparticles (NPs) have potential uses in cancer therapies due to their size (1–100 nm). Nanoparticles are stabilized chemically or via the use of plants. Moringa oleifera (MO), found widely in SA, possesses medicinal properties with MO leaves (MOE) and flowers (MF) with high nutritional val...
Conference Paper
Full-text available
http://dx.doi.org/10.1289/ehp.isee2016 Abstract Number: P2-065 | ID: 4157
Conference Paper
Full-text available
Moringa oleifera gold nanoparticles modulate oncogenes, tumor suppressor genes and caspase-9 splice variants in A549 cells Charlette Tiloke 1, 3, Alisa Phulukdaree 1, 2, Krishnan Anand 3, Robert M Gengan 3 and Anil A Chuturgoon 1 1 University of KwaZulu-Natal, South Africa 2 University of Pretoria, South Africa 3 Durban University of Technology, S...
Article
Full-text available
This study compared phenotypic and genotypic identification methods of Campylobacter spp. against the polymerase chain reaction (PCR) in terms of sensitivity, specificity, positive-predictive value and negative-predictive value. Thermophilic Campylobacter isolates were identified using conventional biochemical tests, specifically hippurate hydrolys...
Article
T-helper (Th) 17 cells are a pro-inflammatory subset of CD4+ effector T-cells critical in mucosal immunity. Imbalances in Th17 cell proportion have been implicated in the pathogenesis of several diseases; however, this has not been adequately explored in tuberculosis (TB) and human immunodeficiency virus (HIV) co-infection. Since Th17 cells are pre...
Article
Lon protease, an ATP dependent mitochondrial protease, is important in mitochondrial protein maintenance. Disruption of protein homeostasis and mitochondrial dysfunction is associated with lipodystrophy, metabolic syndrome and accelerated aging, and are commonly observed in patients on long term antiretroviral therapy. Sirtuin 3 (SIRT3) is a post-t...
Article
Full-text available
Esophageal cancer (EC) is commonly diagnosed in South Africa (SA), with high incidences occurring in SA's black population. Moringa oleifera (MO), a multipurpose tree, is used traditionally for its nutritional and medicinal properties. It has been used for the treatment of a variety of ailments, including cancer. We investigated the antiproliferati...
Article
Full-text available
Testicular toxicity has been implicated in highly active anti-retroviral therapy (HAART) treatment. Hence there is need to identify an effective antioxidant product that can alleviate testicular necrosis due to HAART administration. Forty eight adult male Sprague-Dawley rats were used in this study. The animals were divided into eight (8) groups: A...
Article
Full-text available
Background: Fenugreek (Trigonella foenum-graecum) is globally recognized for its medicinal properties and hypoglycemic effects. The seed extract as well as its active compound, 4-hydroxyisoleucine (4-OH-Ile), have been shown to reduce hyperglycemic insulin resistance. The mechanism by which this occurs has not been investigated in human liver cell...
Article
Full-text available
Aim: We investigated the antiproliferative effects of AuNP synthesised from MO aqueous leaf extracts (MLAuNP ) in A549 lung and SNO oesophageal cancer cells. Methods: A one-pot green synthesis technique was used to synthesise MLAuNP . A549, SNO cancer cells and normal peripheral blood mononuclear cells (PBMCs) were exposed to MLAuNP and CAuNP to...
Article
Full-text available
The ATP-binding cassette (ABC) super-family of drug transporters regulates efflux of xenobiotic compounds. The subfamily, multi-drug resistance proteins (MRPs) transports cyclic nucleotides and xenobiotics. Epigenetic modulation of drug transporters is scarcely described. The regulatory role of microRNA (miR)-124a on drug transporter gene ABCC4 was...
Article
Full-text available
Increased death rates due to lung cancer have necessitated the search for potential novel anticancer compounds such as carbazole derivatives. Carbazoles are aromatic heterocy-clic compounds with anticancer, antibacterial and anti-inflammatory activity. The study investigated the ability of the novel carbazole compound (Z)-4-[9-ethyl-9aH-carbazol-3-...
Article
Full-text available
Ochratoxin A is a nephrotoxic mycotoxin produced by Aspergillus and Penicillium fungi. It contaminates human and animal food products, and chronic exposure is associated with renal fibrosis in humans (Balkan endemic nephropathy). Resveratrol, a phytoalexin, possesses anti-cancer and antioxidant properties. We investigated the mechanism of cellular...
Article
Full-text available
Hyperglycemia exacerbates the production of mitochondrial reactive oxygen species and this contributes to a variety of pathological conditions. Sirtuin 3 (SIRT3) has been shown to play a role in decreasing oxidative stress and improving disease outcomes by regulating antioxidant defense. Our understanding of molecular events during oxidative stress...
Article
Full-text available
Atorvastatin is used to control cholesterol and lipid levels in hyperlipidaemic and hypercholesterolaemic patients. Myopathy and hepatotoxicity, however, have been reported as side effects in a small percentage of statin users. This study aimed to investigate the cytotoxicity and the effect of atorvastatin on microRNA expression in HepG2 cells. The...
Article
Chronic HIV treatment with antiretroviral drugs has been associated with adverse health outcomes. Mitochondrial toxicity exhibited by nucleoside reverse transcriptase inhibitors (NRTIs) is pinpointed as a molecular mechanism of toxicity. This study evaluated the effect of NRTIs: Zidovudine (AZT, 7.1μM), Stavudine (d4T, 4μM) and Tenofovir (TFV, 1.2μ...
Conference Paper
The antiproliferative effect of Moringa oleifera leaf extract on human oesophageal cancer cells Charlette Tiloke, Alisa Phulukdaree, Anil A. Chuturgoon Discipline of Medical Biochemistry and Chemical Pathology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa Abstra...
Article
Full-text available
Chronic air pollution exposure during pregnancy can cause oxidative stress leading to adverse birth outcomes. The aim of this study was to assess and compare oxidative stress response in peripheral lymphocytes isolated from pregnant women from a highly industrialized locale (south Durban (SD); n = 50) and a control with lower air pollutant levels (...
Conference Paper
Full-text available
Methylenetetrahydrofolate Reductase C677T polymorphism is associated with increased risk of coronary artery disease P. Ramkaran*, A. Phulukdaree*, S. Khan#, D. Moodley* and A.A. Chuturgoon* *Discipline of Medical Biochemistry and Chemical Pathology, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa #Departmen...
Conference Paper
Full-text available
MicroRNA-499 Single Nucleotide Polymorphism in Patients with Premature Coronary Artery Disease P. Ramkaran*, A. Phulukdaree*, S. Khan#, D. Moodley* and A.A. Chuturgoon* *Discipline of Medical Biochemistry and Chemical Pathology, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa #Department of Cardiology, Nels...
Conference Paper
Full-text available
Mitochondrial DNA polymorphisms 5178 C>A and 16189 T>C in young South African Indians with coronary artery disease P. Ramkaran*, A. Phulukdaree*, S. Khan#, D. Moodley* and A.A. Chuturgoon* Discipline of Medical Biochemistry and Chemical Pathology, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa #Department...
Conference Paper
Full-text available
Sirtuin 1 polymorphisms in young South African Indians with coronary artery disease P. Ramkaran*, A. Phulukdaree*, S. Khan#, D. Moodley* and A.A. Chuturgoon* *Discipline of Medical Biochemistry and Chemical Pathology, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa #Department of Cardiology, Nelson R. Mand...
Article
Stable gold nanoparticles (AuNPs) from aqueous 1 M chloroauric acid and Moringa oleifera flower aqueous extract. The UV–vis spectrum of AuNPs displays the surface plasmon resonance at 540 nm. The AuNPs was characterized using transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray analysis and dynamic light scatterin...
Article
Full-text available
The −308 G/A promoter polymorphism in the tumor necrosis factor-alpha (TNF-α) gene has been extensively studied as a potential biomarker for pregnancy outcomes, but results tend to be population specific. The aim of this study was to evaluate the association of the TNF α-308 polymorphism with preterm birth (PTB) and low birth weight (LBW) in a coho...