Alimuddin Alimuddin

Alimuddin Alimuddin
Bogor Agricultural University | IPB · Department of Aquaculture

Professor
Marker-assisted selection, transgenesis, protein recombinant, nutrigenomics, transcriptomics

About

179
Publications
85,791
Reads
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703
Citations
Introduction
- transcriptomic related to nutrient metabolism, stress and disease - genetic improvement: marker assisted selection, sex manipulation, gene manipulation
Additional affiliations
October 2013 - present
Bogor Agricultural University
Position
  • Professor (Assoc.)
Description
  • Fish Genetic Manipulation
April 2000 - March 2006
Tokyo University of Fisheries
Tokyo University of Fisheries
Position
  • PhD Student
March 2000 - March 2006
Tokyo University of Marine Science and Technology
Position
  • PhD Student

Publications

Publications (179)
Book
Full-text available
Ilmu biologi, fisiologi, reproduksi, dan pertumbuhan yang ditunjang oleh perkembangan biologi molekuler merupakan paduan ilmu dasar dan terapan dalam pengelolaan perikanan serta produksi benih dan ikan konsumsi yang berkesinambungan. Pengelolaan perikanan terkadang dianggap sebagai reaksi terhadap ”common failure” akibat overfishing, dan budidaya d...
Article
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Fructooligosaccharides (FOS) are natural feed additives with potential application in feed for Pacific white shrimp. This study aimed to evaluate the effects of prebiotic FOS supplementation on the modulation of metabolism-related gene expression and growth performance of Pacific white shrimp. A trial feed consisted of commercial feed supplemented...
Article
Full-text available
Abstract. Irmawati, Meimulya, Tassakka ACMAR, Nadiarti, Rukminasari N, Kadriah IAK, Nasrullah H, Alimuddin. 2022. Identification, genetic diversity, and comparative evolution of the striped snakehead Channa striata (Bloch, 1793) in Wallacea. Biodiversitas 23: 3318-3328. Striped snakehead, Channa striata populations have declined in some areas of Wa...
Article
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Heat-shock protein 70 (HSP70) is a member of the Heat-shock protein family that plays an important role in cellular homeostasis against various stressors in fish. This research aimed to identify the full length of cDNA sequence HSP70 in African catfish C. gariepinus and assessed its mRNA expression under various tissues and stress conditions. The c...
Article
Indonesian carp production has decreased due to mass mortality caused by herpesvirus koi infection. Safe prevention techniques can be applied en masse and are relatively inexpensive are needed. This study aimed to examine the effect of immunization using anti-koi herpesvirus (KHV) DNA vaccine at a dose of 1.3 × 108 cfu.mL ⁻¹ through immersion with...
Article
This study evaluated the survival rate (SR), cannibalism, and growth performance in juveniles African catfish reared in photoperiod conditions and administered with different doses of 17β‐oestradiol (E2). A completely randomized design was used with four replicates in a 3 × 2 factorial, three photoperiods (24L:0D, 12L:12D, 0L:24D) and two levels of...
Article
Full-text available
Induction of triploid striped catfish (Pangasianodon hypophthalmus) through physical shock to obtain a high-quality seed has been done, but the physical shock reduces hatching rates and limits the number of offspring. Cross-breeding of tetraploid with diploid striped catfish is expected to be a solution in overcoming these problems. This trial of n...
Article
This study aimed to compare the phenotypic and genotypic characteristics, and pathogenicity of Edwardsiella ictaluri derived from several catfish farming in Indonesia. The isolated bacteria were from West Java, East Java, South Sumatra, Jambi, Lampung, Riau and South Kalimantan. The phenotypic analysis based on conventional biochemical characterist...
Article
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Giant gourami (Osphronemus goramy) has been known to have a high ability to utilize high dietary carbohydrates. However, its molecular information related to carbohydrate metabolism is still limited. The present study elucidated Giant gourami's liver transcriptome and molecular responses to high dietary carbohydrates. Two semi-purified diets with d...
Article
This study aims to evaluate the culture performance of tilapia (Oreochromis niloticus) that have been treated with 17α-methyltestosterone (MT) and without MT (w-MT), feed with different protein levels (20, 24, and 28%) and recombinant Epinephelus lanceolatus growth hormone (rElGH)-diet. The research was conducted in 9 treatments and triplicate. Til...
Article
Full-text available
The dietary supplementation of probiotics Bacillus sp. NP5 in female African catfish Clarias gariepinus (Burchell 1822) resulted in the improvement of the reproduction performance of the fish in one of our previous studies, but its effects on the male fish have not been evaluated yet. Thus, this study aimed to evaluate the effect of dietary Bacillu...
Article
Tilapia (Oreochromis niloticus) is one of the freshwater fish that is of great interest to the public, because its protein content is quite high, namely 55.58%. One of the problems in tilapia cultivation is that the need for feed is very high, where only 25% of the feed given is converted as a result of production and the rest is wasted as waste. T...
Article
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The Indonesian leaffish Pristolepis grootii (Bleeker, 1852) is an indigenous fish species. The purpose of this study was to analyze the characteristics of the β-subunit of follicle-stimulating hormone (FSH-β) and the β-subunit of luteinizing hormone (LH-β) of P. grotii. Complementary DNA (cDNA) was synthesized from mRNA that was extracted from P. g...
Article
Tujuan penelitian ini adalah untuk mengevaluasi efektivitas suplementasi ekstrak serbuk sari pinus melalui pakan terhadap maskulinisasi dan kinerja reproduksi ikan gapi. Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri atas lima perlakuan dengan tiga ulangan, yaitu meliputi suplementasi 10 mg ekstrak serbuk sari pinus per kg pak...
Article
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Background: RNA interference (RNAi) has recently introduced as a powerful technique for specific gene silencing in antiviral therapy for controlling shrimp diseases. The present study was conducted to assess the effect of dsRNA VP15-WSSV vaccine by oral administration on tiger shrimp survival rate and response immunes. Methods: A gene encoding VP15...
Article
Full-text available
Growth improvement of the giant gourami through molecular assisted selection offers a breakthrough solution regarding the slow growth problem in culturing the fish species. However, gene molecular expression studies and gene mapping information are scarce for this species. This study aimed to evaluate the growth, expression of the growth-related ge...
Article
Full-text available
Indonesian leaffish, Pristolepis grootii (Bleeker, 1852), is an undomesticated freshwater fish species native to the rivers, flooded swamps, and tributaries of Indonesia. The fish is mainly captured for consumption. In order to prevent its extinction and supply its growing demands, the artificial breeding of the fish should be developed. The purpos...
Article
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Highlight ResearchThe F2 of GH-transgenic B. imbellis was successfully producedThe transgene inheritance by the F2 fish was more than 90%The growth and body size of transgenic fish was significantly higher than controlF2 fish reached a larger body size in a shorter period compared to the F1 AbstractIn our previous research, we had successfully prod...
Article
Full-text available
This study was aimed to evaluate the use of corn steep powder (CSP) as a plant protein source in Oreochromis niloticus diet. A commercial feed with 28% protein content and 368 kcal/g energy was used as reference diet, while the test feed consisting of various CSP content, namely 0%, 10%, 20%, and 30% and feed containing soybean meal (SBM) at the le...
Article
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Red color coverage (RCC) is a commercial trait developed and refined to improve the appearance of many ornamental fish commodities. In neon tetra, the status of variation of RCC is not yet investigated or reported. This study aimed to analyze the RCC variation of cultured neon tetra as a basis for breeding strategies. A total of 900 neon tetras (st...
Article
Full-text available
Carp is one of the leading freshwater aquaculture commodities in Indonesia. Further improvement of carp strains by the Research Institute for Fish Breeding (RIFB), Sukamandi, Indonesia, has produced a synthetic F2 carp. The strain is assembled from different strains of carps and has shown better growth and health characteristics. Considering that h...
Book
Budidaya di tambak dan keramba jaring apung (KJA) tergolong budidaya air payau (brackishwater aquaculture) yaitu budidaya yang diusahakan pada air bersalinitas 0,5-30 g/L. Budidaya air payau skala besar umumnya bersifat single-species dengan komoditas utama adalah udang. Metode tersebut berbasis input tinggi sehingga kelestarian lingkungan dipertan...
Article
Full-text available
Male giant freshwater prawn grows faster than its female. Therefore, male mono sex culture is one of the solutions to improve aquaculture production. The all-male population of giant freshwater prawns can be produced by mating the neo-females (sex-reversed males) with the normal males. This study was aimed to identify the molecular markers related...
Article
Full-text available
An effort to increase the production of juvenile catfish is limited by the high mortality rate, especially caused by cannibalism. The hormonal treatment has been conducted as an effort to control cannibalism. This study used completely randomized factorial design, consisted of six treatments and three replications. There were two factors examined i...
Article
This study aimed to evaluate the effect of creatine and cysteamine supplementation on the growth performance, fillet quality and resistance to bacterial infection of Streptococcus agalactiae in red tilapia. Five types of experimental diet (quadruplicate) with the same energy and protein content were tested. These consist of N (negative control), A...
Article
Full-text available
Understanding the development of immunity in the early life of fish may help improve the effective management of diseases in aquaculture. However, little is known about the timing of the appearance of different immune-related genes in African catfish C. gariepinus, an important aquaculture species in Africa and Southeast Asia countries. The current...
Article
Full-text available
Striped catfish tetraploid induction through heat shock that carried out in this study is not intended to produce on growth fries.But to form a broodstock to produce triploid on growth fries through cross breeding without any physical shock induction process.The fries from the heat shock induction in the previous study were screened or selected by...
Article
Full-text available
Striped catfish tetraploid induction through heat shock that carried out in this study is not intended to produce on growth fries.But to form a broodstock to produce triploid on growth fries through cross breeding without any physical shock induction process.The fries from the heat shock induction in the previous study were screened or selected by...
Article
Full-text available
In order to lower the fish mortality during transportation, the chopped banana stem (BS) was added during the African catfish transport procedure. The fish were packed in plastic bags with a density of 100 fish in 1.5 L water. The chopped BS was added into the plastic bags with different concentrations: 0 (control), 5, 10, and 15 mg L ⁻¹ . The fish...
Article
The present study aimed to evaluate the growth performance, immune response, and immunity-related genes in white shrimp given the Bacillus sp. (NP5) probiotic and honey prebiotic and V. parahaemolyticus infection. This study consisted of five treatments: NP5, honey, synbiotic (honey and NP5), positive control (PC), and negative control (NC), respec...
Article
Full-text available
White spot syndrome virus (WSSV) is a significant cause of tiger shrimp mortality and has a profound economic impact on commercial shrimp farming. A transgenic technology provides an effective method to control the viral disease by increasing shrimp resistance. The study aimed to assess the overexpression of the PmAV (Penaeus monodon antiviral) gen...
Article
The experimental diets containing five levels of creatine supplementation, namely control (N = 0 g/kg creatine), B1 (1.00 g/kg glycine), B2 (0.40 g/kg creatine), B3 (0.80 g/kg creatine) and B4 (1.20 g/kg creatine) were tested to red tilapia up to apparent satiation 3 times a day for 56 days. After the feeding trial, fish were stressed by air exposu...
Article
Full-text available
Glucokinase (GCK) is one of the enzymes that play important roles in carbohydrate metabolism and high glucose homeostatic in fish. The information about the GCK mRNA sequence and its expression is limited in Giant gourami, one of the most important herbivorous aquaculture species in Indonesia. The present study aimed to characterize the GCK mRNA an...
Article
Full-text available
Coral platy fish has a unique reproduction due to ovoviviparous (live-bearer) reproduction. The large scale production of coral platy fish has several constraints due to the inconsistent seed birth period, which causes variations in the fry size. This makes it difficult for ornamental fish cultivators for production process efficiency and market cr...
Article
The present study evaluated the effects of cysteamine supplementation on the serum insulin and somatostatin, IGF-1 and GLUT4 genes expression, growth performance and robustness against stress in red tilapia (Oreochromis sp.). This study tested five cysteamine dietary levels (quadruplicate), i.e. treatments consisted of control (N = 0 g/kg of cystea...
Article
Full-text available
The first step to establishing appropriate broodstock management and conservation programs and to avoid decreasing genetic variability in this selective breeding program is the development of molecular genetic markers that can be used for evaluation of genetic stock determinations in gene flow rates. However, morphologically it is rather difficult...
Conference Paper
Full-text available
The Asian seabass, Lates calcarifer (Perciformes, Latidae), known as barramundi in Australia, as salamata and bale kanja in South Sulawesi, was first described by Bloch in 1790 under the name Holocentrus calcarifer. The euryhaline L. calcarifer is a high-value marine and freshwater fish, on a par with salmon and tuna. This species has a widespread...
Article
Hasanah U, Binawanto, Alimuddin A, Boediono A, Kursini E. 2020. Short Communication: Effect of cryopreservation on ultrastructure and mitochondrial function of albino Pangasius catfish spermatozoa. Biodiversitas 21: 4524-4528. Cryopreservation techniques have been carried out on many endangered species and animals with unique characteristics. Succe...
Article
Full-text available
Ardana Kurniaji, Sri Nuryati, Alimuddin, and Sri Murtini. 2020. Gonad development and blood profile of anti-KHV DNA vaccinated common carp. Aquacultura Indonesiana, 21 (2): 49-55. Koi herpesvirus (KHV) is one of pathogen that infects common carp. Alternative to prevent KHV infection is trough vaccination This study aimed to observe gonad developmen...
Article
Viral nervous necrosis (VNN) is a disease that often infects groupers. It has caused mass death in more than 34 species of marine fish. DNA vaccination might become a solution againts the infection. The construction of the pmBA-CP DNA vaccine consisting of the beta-actin promoter of medaka fish (Oryzias latipes) and capsid protein (CP) encoding VNN...
Article
Full-text available
GP-11 KHV DNA vaccine is a vaccine that can be used to induce immunity against the KHV virus (Koi herpesvirus). Vaccination through feed is an alternative way of administering vaccines. The study aimed to examine the effect of giving KHV GP-11 DNA vaccine through feed with different frequencies to KHV infection. The frequency of vaccine administrat...
Article
Full-text available
Vaccination is a practical step in preventing diseases caused by koi herpes virus (KHV) in koi fish (Cyprinus carpio haematopterus). We have developed two DNA vaccines for KHV named as GP-25 and GP-11 from two local isolates coded as ORF25 and ORF81, respectively. Although both vaccines have been reported to increase survival rates, the evaluation...
Article
The severe mortality of fish due to the infection of megalocytivirus caused significant economic losses. Since 2011, megalocytivirus (giant gourami iridovirus (GGIV)) has become the main pathogen in giant gourami (Osphronemus goramy), particularly in West Java, Central Java and Bali. This study aimed to develop primary cell culture from spleen as t...
Article
Full-text available
Megalocytivirus of family Iridoviridae has been identified as a pathogen that caused the fatal systemic infection to lead to massive death of numerous fish species in both ornamental and food fish industries. It caused significant economic losses due to severe mortality of infected fish. Since 2011 in Indonesia, megalocytivirus in giant gourami (Os...
Article
Full-text available
Siput gonggong Laevistrombus turturella merupakan siput laut yang banyak dikonsumsi masyarakat di Provinsi Kepulauan Riau sehingga populasinya diperkirakan menurun. Upaya pelestarian siput ini perlu dilakukan salah satunya dengan budidaya. Kegiatan budidaya memerlukan ketersediaan induk matang gonad. Tujuan penelitian ini adalah menganalisis ukuran...
Article
Hidayani AA, Fujaya Y, Trijuno DD, Rukminasari N, Alimuddin A. 2020. Genetic diversity of blue swimming crab (Portunus pelagicus Linnaeus 1758) from Indonesian waters (Sunda and Sahul Shelf, Wallacea region): Phylogenetic approach. Biodiversitas 21: 2097-2102. Blue swimming crab is one of the exploited fisheries commodities in Indonesia. This condi...
Article
Full-text available
Vaccination is an efficient step in preventing diseases caused by koi herpesvirus (KHV) in koi fish (Cyprinus carpio haematopterus). We have developed the DNA vaccine for KHV from the ORF25 and ORF81 from the local isolate, namely GP-25 and GP-11 vaccine, respectively. Although it has been reported to increase survival rates, the evaluation of koi...
Article
Full-text available
ABSTRAK Penelitian ini bertujuan untuk menganalisis diferensiasi morfologi yang terjadi di antara stok ikan kakap putih, barramundi (Lates calcarifer Bloch, 1790) di perairan pantai Kabupaten Bone, Wajo, Takalar, dan Kalimantan Utara. Diferensiasi karakter morfometrik dianalisis menggunakan fungsi dikskriminan. Hasil penelitian menunjukkan bahwa ik...
Article
Full-text available
This study was aimed to optimize the spermatozoa cryopreservation protocol in Albino pangasius catfish by assessing the effectiveness of different cryoprotectant concentrations and different time of equilibration. The first experiment was conducted to optimize the combination of 10% methanol with different concentration of skim milk (0%, 5%, 10%, 1...
Article
Full-text available
Aquaculture of the African catfish Clarias gariepinus has rapidly increased in South-East Asia over recent years, which has now become one of the most cultivated species. This culture intensification has resulted in the development of various diseases, among which the Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The present st...
Article
Full-text available
Fermentation liquid from mangrove leaves Avicennia marina contains microorganisms, nutrients, and secondary metabolites. This study aimed to identify bacteria and the compounds in fermentation liquid of mangrove leaves A. marina and measured their inhibitory capacity against pathogenic bacteria Stenotrophomonas maltophilia which causes ice-ice dise...
Article
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Nasrullah N, Nababan YI, Safitri I, Yanti DH, Nuryati S, Zairin Junior M, Alimuddin A. 2020. Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus. Biodiversitas 21: 311-317. The chicken-type lysozyme (LYSC) gene has been demonstrate...
Article
Full-text available
Gonggong adalah sejenis siput laut yang merupakan makanan laut (seafood) favorit dan ikon Kota Tanjungpinang, ibukota Provinsi Kepulauan Riau (Kepri). Gonggong mengandung protein tinggi, yaitu sekitar 46,65%. Tidak ada laporan tentang produksi budidaya dan upaya konservasi gonggong. Teknologi produksi benih buatan gonggong belum berkembang. Tujuan...
Preprint
Full-text available
Selective breeding and management broodstock of barramundi (Lates calcarifer)
Article
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Siput gonggong Laevistrombus turturella semakin intensif ditangkap sehingga populasinya di alam menurun. Upaya budidaya gonggong diperlukan untuk menjaga populasinya. Teknologi reproduksi siput belum banyak dikembangkan di Indonesia. Keberadaan 17β-estradiol pada siput dan moluska lain telah dinyatakan oleh beberapa peneliti, namun perannya dalam p...
Conference Paper
Full-text available
Koi herpesvirus (KHV) adalah virus yang sangat virulen dan dapat menginfeksi ikan mas (Cyprinus carpio) mulai dari stadia larva sampai dewasa. Salah satu alternatif adalah dengan meningkatkan proteksi ikan melalui induksi imunitas maternal. Penelitian ini bertujuan untuk menguji sensitivitas pada tahap awal perkembangan ikan mas yang diperoleh dari...
Article
Full-text available
This study aims to determine the influence of initial shock treatment based on measuring zygote DNA content in striped catfish (Pangasianodon hypophthalmus) tetraploid induction. The study applied a completely randomized design. The shock was administered at the zygote age of 28, 28.5, 29, 29.5, 30 and 30.5 minutes after the fertilization process,...
Article
Full-text available
Lysozymes play an important role in the first line of defense in fish and potentially used as an immunity status biomarker and immune responses evaluation in fish, which often found in two types, i.e. chicken-type and goose-type (c- and g-type, respectively). To recent, the information related to the sequences and the expression analysis of the c-...