Alim Isnansetyo

• Faculty Member at Universitas Gadjah Mada

Agarolytic bacteria produce agarase, which may aid in the growth of cultured tropical abalone fed natural seaweed. Agarolytic bacteria can come from a variety of sources, such as seawater, abalone intestines, and dead seaweed. This study aimed to isolate, screen, describe, and identify agarolytic bacteria found in red macroalgae. Agarolytic bacteri...

With trends towards low fishmeal and higher plant ingredient-based diets for shrimp, there is consequently greater demand on feed additives to bolster the protein and nutrient profile quality for achieving more efficiency. Also, there is a need to enhance innate immunity and resilience to infection under intensive shrimp production scenarios. This...

Highlight Research 1. Lymphocystis disease is reported to infect seawater and freshwater fishes 2. The four important freshwater fish species in Indonesia are evaluated on their susceptibility to Lymphocystis Disease Virus 3. Lymphocystis infection causes behavioural changes and mortality with different onset times after infection on the four fish...

Pseudoalteromonas viridis strain BBR56 was isolated from seawater at Dutungan Island, South Sulawesi, Indonesia. Bacterial DNA was isolated using Promega Genomic DNA TM050. DNA purity and quantity were assessed using NanoDrop spectrophotometers and Qubit fluorometers. The DNA library and sequencing were prepared using Oxford Nanopore Technology Gri...

Vibriosis is a disease that frequently infects shrimp and financially devastates the shrimp industry worldwide. Because of the detrimental effects of antibiotic use in shrimp disease management, such as the emergence of various antibiotic-resistant bacteria, decreased antibiotic efficacy due to long-term use, and residues remaining in sediments, wa...

Prokaryotic and eukaryotic organisms contain heat shock proteins (HSPs), crucial for rapid response to environmental stress. However, their specific roles in different stress conditions are not fully understood. This study investigated HSP70 and HSP90 expression in H. diversicolor squamata, using qRT-PCR. Data analysis employed SPSS, including t-te...

Getting an efficient extraction approach is a crucial step in bioactive protein research, particularly lectin. This research aimed to examine the efficiency of cryogenic-grinding (CG) and freeze-dried-grinding (FG) pre-extraction treatments, and also the incorporation of phenylmethylsulphonyl fluoride (PMSF), Tween 80, polyvinylpolypyrrolidone (PVP...

This study aimed to determine the effect of probiotics on the intestinal digestive enzyme, morphology and total bacteria of red tilapia (Oreochromis sp.) fed maggot BSF (Hermetia illucens) larvae. The research was conducted using randomized design of two treatments (with probiotics and without probiotics) in three replications. Tilapia (average wei...

Bacterial vaccines have been tested for their effectiveness in protecting fish from potential virulent diseases. The current vaccine preparations are mostly in liquid form, which affects prices due to an increase in the cost of shipment, the stability of liquid products also has limitations in long-term storage. This study aims to develop a method...

Nitrifying bacteria play a role in reducing the amount of toxic ammonia in the rearing media. The purpose of this study was to characterize, and identify nitrifying bacteria isolated from shortfin eel ( Anguilla bicolor ) recirculation aquaculture system. The selective anorganic nitrifying medium was used to isolate the bacteria, followed by cultiv...

The purpose of this study was to investigate species specific inhibitory effects of esDNA isolated from two conspecific organisms: Vibrio parahaemolyticus (VP) and Vibrio harveyi (VH), and to assess the functional role of esDNA to enhance the survival rate of Artemia sp. In an in vitro study, nine doses of Extracellular self-DNA of Vibrio parahaemo...

Mulia DS, Rahayu SD, Suyadi A, Mujahid I, Isnansetyo A. 2023. Antibacterial activity of mangrove plant extract of Rhizophora apiculata in inhibiting the growth of various strains of Aeromonas hydrophila. Biodiversitas 24: 4803-4810. Aeromoniasis is a bacterial disease caused by Aeromonas hydrophila. Synthetic antibiotics are often used to treat thi...

Background and Aim The high diversity of Aeromonas spp. results in various pathogenicity levels. This group of bacteria causes a serious disease named motile Aeromonas septicemia (MAS) in catfish (Clarias spp.). This study aimed to characterize the species and virulence gene diversity of Aeromonas spp. isolated from diseased catfish. Materials and...

Immunostimulants are more suitable for improving the health of shrimp than using drugs in the prevention of diseases. This study evaluated the efficacy of bioactive compounds from Ocimum basilicum and Piper betle in increasing the non-specific immune response of Litopenaeus vannamei . Samples were collected directly from a traditional market. Shrim...

Present research aimed to examine the pathogenicity opportunities of some bacteria which were isolated from freshwater aquaculture ponds to some fish Oreochromis niloticus, Clarias gariepinus, and Pangasius pangasius. The bacteria were isolated from the water and feces of O. niloticus and C. gariepinus aquaculture in Samarinda, which were identifie...

Istiqomah I, Isnansetyo A, Murwantoko, Handayani DP, Lestari YN, Taslihan A, Permana IGN, Wijayanti E. 2023. Antibiotic resistance of emerging pathogenic bacteria of hybrid grouper farming in Indonesia. Biodiversitas 24: 2493-2501. A hybrid grouper is among the main coastal farming species in Asian countries, yet vibriosis is still a significant is...

Two separate trials were conducted to evaluate the use of a novel Corn Fermented Protein (CFP) on growth performance and feed utilization of Pacific white legged shrimp Litopenaeus vannamei in out-door pond conditions as well an independent pathogen challenge (Vibrio harveyi) test under controlled conditions following an internal feeding trial with...

Highlight Research Development of immunostimulant from natural sources is a promising approach to improve the immune status of fish Dietary administration of sodium alginate from Padina australis at 2.0 g/kg was an effective immunostimulant for red tilapia Red tilapia fed diet with sodium alginate significantly increased innate immune responses of...

A fermented earthworm (FEW) is reported to be an alternative to an immune-modulator feed additive in catfish. However, the effects on growth and feed efficiency have not been reported yet. The present study aimed to evaluate the effects of fermented earthworms (FEW) on the growth and feed efficiency of catfish (Clarias sp.). A feeding trial was con...

Isnansetyo A, Istiqomah I, Anshary H, Sriwulan S, Yudiati E, Subagiyo S, Arif A, Kartikasari DW. 2022. Identification and antibiotic-resistant properties of Vibrio owensii and V. alginolyticus isolated from the Spermonde Islands, Indonesia. Biodiversitas 23: 5995-6005. The Spermonde Islands are located in Makassar Strait, southern Sulawesi, Indones...

Microbubble aeration has been recognized as a tool to improve dissolved oxygen and fish culture performance in limited water aquaculture. This research aims to investigate the effect of microbubble aeration on increasing dissolved oxygen, fish culture performance, and stress resistance of red tilapia. The study used 3 treatments of aerations namely...

Fucoidan is a bioactive compound of brown seaweed with antioxidant characteristics. This study examined the aftermath of the extraction method on the yield, fucose content, xylose content, sulfate content, total sugar, antioxidant activity, and functional groups of fucoidan from Sargassum hystrix. The brown seaweed was extracted using 4 methods, na...

The marine bacterium Pseudoalteromonas xiamenensis STKMTI.2 was isolated from a mangrove soil sediment on Setokok Island, Batam, Indonesia. The genome of this bacterium consisted of 4,563,326 bp (GC content: 43.2%) with 1 chromosome, 2 circular plasmids, 2 linear plasmids, 4,824 protein-coding sequences, 25 rRNAs, 104 tRNAs, 4 ncRNAs, and 1 cluster...

Catfish which is an important component of global freshwater aquaculture production is also prone to a wide range of diseases resulting in economic losses. Improvement of the immune status of catfish is a promising preventive approach to control disease. The purpose of this study was to determine the effect of fermented earthworms as a feed additiv...

Isnansetyo A, Handayani DP, Istiqomah I, Arif A, Kaneko T. 2021. An antibacterial compound purified from a tropical coastal plant, Diospyros maritima. Biodiversitas 23: 135-142. This study aimed to isolate, purify, and characterize an antibacterial compound from Diospyros maritima and evaluate its antibacterial activity. Diospyros maritima bark was...

Abstrak Abalon merupakan salah satu moluska bercangkang tunggal yang memiliki nilai ekonomis tinggi dan merupakan komoditas potensial dalam peningkatan devisa Negara. Namun permasalahannya adalah mudahnya abalone mengalami stress akibat perubahan berbagai faktor lingkungan seperti suhu, salinitas, bakteri Vibrio dan bahan desinfektan seperti chlori...

Latar Belakang. Uji sensoris dan uji umur simpan pada pengembangan produk pangan diperlukan agar produk yang dihasilkan dapat diterima dan aman bagi konsumen. Individu yang mengalami prediabetes memiliki kesempatan untuk mencegah atau menunda perkembangan terjadinya diabetes melitus (DM) jika dapat memperbaiki pola hidupnya. Makanan selingan adalah...

This study was conducted to develop Chaguro, a low-cost supplementary food made of chayote (Sechium edule (Jacq.) Swartz) and tuna fish (Thunnus sp.), for diabetes and dyslipidemia diet therapy. In order to find a formula with effective hypoglycaemic and antidyslipidemic properties, dried tuna and chayote were mixed at different ratios: F1 (75% tun...

Penelitian ini bertujuan untuk mengevaluasi kandungan nutrien dan antinutrien tepung daun kelor terfermentasi sebagai sumber protein nabati pakan ikan. Tahap penelitian meliputi pembuatan tepung daun kelor, persiapan dan penghitungan kepadatan bakteri, proses fermentasi, dan analisis kandungan nutrien dan antinutriennya. Bakteri yang digunakan untu...

Latar Belakang. Pengaturan diet bagi pasien prediabetes merupakan salah satu pencegahan kejadian penyakit diabetes melitus secara dini. Labu siam dan ikan tuna merupakan bahan pangan lokal yang mengandung banyak senyawa di mana mampu menanggulangi masalah diabetes sehingga dapat dimanfaatkan sebagai makanan tabur fungsional. Pengembangan makanan be...

Algae (Ulva sp.) and tuna (Thunnini) are edible marine resources rich in protein, essential amino acids, zinc, and iodine that can be used to improve the growth of malnourished children including their cognitive functions. The combination of both resources may produce a supplementation (Furikake Ulvamina) with nutritional quality to support the acc...

Lutfia FN, Isnansetyo A, Susudarti RA, Nursid M. 2020. Chemical composition diversity of fucoidans isolated from three tropical brown seaweeds (Phaeophyceae) species. Biodiversitas 21: 3170-3177. Fucoidan is a polysaccharide with high sulfate content, found in the cell walls matrix of brown seaweed. Its bioactivities vary depending on the algae spe...

Survival rate and immunity of white leg shrimp ((Litopenaeus vannamei) postlarvae against acute salinity stress were evaluated in indoor experimental trials. The research was conducted with 2x2 factorial design with two levels of aeration (microbubble and macro bubble) and two levels of diet (with and without probiotics) resulting four treatments i...

Vibrio spp. have been known responsible for fish diseases in marine and brackish‐water systems in the tropics regions. Heat shock proteins are a highly conserved protein group that is known for its rapid response to environmental stresses, including infection. This study aimed to investigate physiological and biochemical responses of tropical abalo...

Tujuan Penelitian ini untuk menganalisis toksisitas daun miana sebagai antibakteri pada udang vanamei. Penelitian ini dilaksanakan pada Maret sampai Oktober 2019, di Laboratorium Sekolah Tinggi Teknologi Kelautan (STITEK) Makassar. Sampel daun miana berasal dari beberapa daerah di Sulawesi Selatan. Uji aktivitas antibakteri ekstrak daun miana denga...

Vibriosis is a bacterial disease that has been reported in Indonesian marine fish culture since the 1990s. The disease was reported mostly in grouper and shrimp (monodon and vanname) farming, although the infections in snapper ( Lates calcarifer) and abalone ( Haliotis squamata) aquaculture were also occurred. Causative agents of vibriosis in Indon...

Aim of this study was to isolate, characterize, and identify cellulolytic bacteria from the digestive tract of marine vertebrates and invertebrates as a candidate of fish probiotics. The bacteria were isolated from the digestive tract and grown on a cellulose agar plate. The bacteria were screened based on the cellulolytic activity, acid resistance...

Up regulation of heat shock proteins (HSPs) in organisms can be detected in response to many kinds of stressor. Recently there were few studies have focused on the three kinds of heat shock proteins (HSPs) and antioxidant enzyme activity after biofloc stress. The objective of this study was to investigate the effect of different biofloc volume on t...

Seaweeds are potential foodstuff and source of bioactive substances that are very useful in the food and pharmaceutical industries. The objective of this study was to evaluate toxicity of aqueous extracts of seaweeds by Brine shrimp lethality test (BLT) and cytotoxicity test. The seaweeds used in this study were Enteromorpha sp. and Laurencia sp. c...

Although alginate is known as an immunostimulant in shrimp, the comprehensive and simultaneous study on its activity to resolve the relationship of the hematological parameters, upregulation of immune-related gene expression, and resistance to pathogen has not been found in shrimp. We performed experiments to evaluate the effect and mechanism of al...

This study was conducted to determine the influence of Sargassum hystrix powder (SHP) as an alternative source of functional food for treating in vivo stress by measuring levels of glucose, triacylglycerol, total cholesterol, and cortisol, and liver histopathology. Wistar rats aged 3 months and weighing 150~200 g were divided into 7 groups: normal...

An increase in temperature can be a significant stressor for aquatic organisms. Abalones, a type of single-shelled gastropod, are the most important commercial species in shellfish cultivation in Indonesia. To evaluate the potential ecological risks posed by temperature stress, we measured biological responses such as survival rates, mucus producti...

The Musi River is located in the southern Sumatra, Indonesia. Most of activities, i.e. agricultural, industrial, and urban activities are considered as being major sources of chemicals and nutrients with their waste products effluent into the river. Nitrification, the microbial oxidation of ammonia to nitrite and nitrate, occurs in a wide variety o...

Introduction: Immunomodulator are chemical compounds that can improve the body’s defense mechanisms both specific and non-specific, and non-specific induction of both cellular and humoral defense mechanisms. Objectives: The objectives of this study were to investigate immunomodulator activity and to identify the chemical constituents of active frac...

In this study the crude ethyl acetate extracts and fractions from red algae Gracilaria edulis were studied against fish pathogens. G. edulis was extracted with ethyl acetate, fractionated in silica gel coloum chromatography and gradient hexane-ethyl acetate. The extract and fractions were subjected for bioassay guided isolation against fish pathoge...

Objective: This study aimed to determine the effect Sargassum hystrix (S. hystrix) powder on the levels of glucose, lipid profile and pancreatic profile of diabetic wistar rats. Methodology: S. hystrix powder (SHP) was made by grinding and sieving to obtain a 120 mesh sized powder. The diabetic rats were administered 450, 600 and 750 mg kgG¹ doses...

This research aims to know antioxidant activity, total phenolic, nutrient, and screening phytochemistry compounds of S. hystrix dry powder. Water extract from the dried sample of brown algae (S. hystrix) was prepared and examined for its phenolic compounds. The amount of total phenolic compounds in water extract was about 11.43 g Gallic Acid Equiva...

This research was aimed to identify the brown seaweed, to characterize the acid, sodium and calcium alginate, and to examine the alginate yield. The identification was done phaenotypically. The extraction method was pretreated by ethanol depigmentation, followed by the extraction of Na2CO3/EDTA and CaCl2 and presipitated with absolute ethanol. The...

Introduction: Fucoidan is a sulfated polysaccharide that has a wide range of bioactivities including anti-cancer. This polysaccharide commonly is extracted from marine brown seaweed. There is lack of information on the fucoidan extracted from tropical brown algae and its anti-cancer activity. Objectives: The objectives of this study were to purify...

Fucoidan is polysaccharide compound containing L-fucose and sulfate ester groups that mainly produced by brown algae. Previous studies have proved that this compound improves the non-spesiic immune parameter of tilapia by injection. The objective of this study was to determine the nonspecific immune parameters response of tilapia by oral administra...

Vibriosis is an important bacterial disease of mariculture industry in the world. The disease can be treated by using common antibiotic, but continuous application of the antibiotic resulting a wide range of negative influence. Exploration of novel antibiotic with narrow spectrum, specific to fish pathogenic Vibrio spp., should be proposed. Indones...

Vibriosis is an important bacterial disease of mariculture industry in the world. The disease can be treated by using common antibiotic, but continuous application of the antibiotic resulting a wide range of negative influence. Exploration of novel antibiotic with narrow spectrum, specific to fish pathogenic Vibrio spp., should be proposed. Indones...

This research aimed to identify ectoparasite and to investigate prevalence and intensity of the infection in the giant freshwater prawn (Macrobrachium rosenbergii de Man) cultured with UGADI method in Sleman. The research was conducted by case study method in two groups of fish farmer (Mina Ulam Asri & Mina Muda Sambe Rembe) which conduct UGADI in...

White shrimp (L. vannamei) is a global aquaculture commodity, which is cultured intensively in around the world including Indonesia. In Indonesia, the shrimp production now is dominated by this species. The shrimp aquaculture frequently faces diseases outbreak mainly caused viral and bacterial pathogens. Non-spesific immune system plays an importan...

Diabetes mellitus (DM) is a metabolic disorder due to insulin deficiency, insulin resistance or inability of β-cells of pancreas to produce insulin. This study was aimed to evaluate the effects of Na-alginate from Turbinaria ornata (Turner) J.Agardh on glucose level and lipid profile in alloxan?induced diabetic rats. Na-alginate was extracted from...

Diabetes mellitus (DM) is a metabolic disorder with symptoms of chronic hyperglycemia and disturbances in metabolism of carbohydrates, fats and proteins due to insulin deficiency, insulin resistance or inability of β-cells of pancreas to produce insulin. This study was aimed to evaluate the effects of ethanolic extract from Sargassum polycystum on...

Fucoidan mainly produced by brown algae is a sulfated polysaccharide with fucose as a main molecule backbone. The objective of this research was to evaluate the non-specific immune potentiating activity of fucoidan from a tropical brown alga, Sargassum cristaefolium indigenous to the Gunungkidul coast, Yogyakarta, Indonesia. Fucoidan was extracted...

Seaweeds produce great variety of metabolites benefit for human. Red seaweeds (Rhodophyta) are well known as producer of phycocolloids such agar, agarose, carragenan and great variety of secondary metabolites. This review discusses the red algal secondary metabolites with antibacterial activity. The chemical constituents of red algae are steroid, t...

Fucoidan is a sulfated polysaccharide from the sea that has a wide range of bioactivities, among others as anti-cancer and immunomodulators. The seaweeds sampling was carried out in the intertidal zone during low tide, followed by the identification, extraction and purification, and cytotoxicity assay. Identification showed that three seaweed used...

The aims of this study were to investigate lymphocyte proliferation activity and to identify chemical constituents of active fractions of star fish Acanthaster planci. A. planci was collected from Ternate Island, North Moluccas, extracted with distilled methanol and water, partitioned with gradient chloroform-hexane-methanol-water and fractionated...

A 42-day indoor feeding trial was conducted to evaluate the growth performance and feed utilization of juvenile white shrimp Litopenaeus vannamei fed with diets containing different supplement levels of hot-water extract of brown tropical macro algae Sargassum cristaefolium extract. The commercial white shrimp feeds containing 36% crude protein wer...

Marine organisms including algae has potential as high source of bioactive secondary metabolite that might be useful in the development agents for pharmaceutical, agriculture and aquaculture. Rhodophyta is one of phylum algae as the richest source of bioactive compounds. This study was conducted to screen the bioactivity secondary metabolites produ...

The Ariake Sea located in the west parts of Kyushu Island is a semi-closed and macro-tidal shallow sea, and has the largest tidal flat in Japan. A large mud tidal flat with a productive ecosystem found along the western shoreline of the sea makes this area ideal as a major production site of nori (Porphyra yezoensis) in Japan. We determined the ind...

To investigate seasonal variations of nutrient distribution in the mudflat–shallow water system, we conducted field surveys once a month from August 2007 to July 2008 in the inner area of Ariake Bay (IAB), Japan. The NH4+–N concentration of the water column increased in autumn because of the high NH4+ release from the sediments, ranging from 850 to...

The purpose of this research was to investigate the nitrification potential in different sediment types in the Ariake Sea tidal flat. The Ariake Sea, a semi-closed shallow sea with macro-tidal and a large tidal flat, is located in the west part of Kyushu Island, Japan. Different kinds of sediment develop in the different parts of its tidal flat. We...

In vitro activity test is a critical evaluation to screen the potential biocontrol agent. We developed a selective medium for quantitative in vitro activity evaluation of bacterial biocontrol agents against pathogenic Vibrio in aquaculture. Sensitivity test of bacterial biocontrol and Vibrio spp. to nine antibiotics showed that oxytetracycline inhi...

The aims of this study were to know the nitrification potential of the mud sediment of the Ariake sea tidal flat, and to investigate the individual influence of environmental parameters such as temperature, pH, salinity and ammonium concentration on its nitrification rate. The Ariake Sea is a semi-closed shallow sea with macro-tidal and the largest...

Leaf area index (LAI) is an important parameter to identify the water balance in forested watershed as a biological factor influencing directly on the evapotranspiration in the forest area. The purpose of this study was to estimate the LAI in a small forested watershed in summer and winter by applying the Terra/Advanced Spaceborne Thermal Emission...

To clarify the mechanism of hypoxia in the western interior parts of the Ariake Sea (WIAS), field observation data collected in the period of 1972–2004 were analyzed using a two-layer box model. Monthly averages of advection velocity, vertical diffusion coefficient (K z ), and biochemical oxygen consumption rate (R) in WIAS were evaluated quantitat...

Pseudomonas is a genus of non-fermentative gram-negative Gammaproteobacteria found both on land and in the water. Many terrestrial isolates of this genus have been studied extensively. While many produce bioactive substances, enzymes, and biosurfactants, other Pseudomonas isolates are used for biological control of plant diseases and bioremediation...

We isolated a marine bacterium strain S2V2 which inhibited the growth of pathogenic marine Vibrio spp. The aims of this research were to identify a new antibiotic-producing marine bacterium strain S2V2, and evaluate its spectrum activity and pathogenic property. Analysis of 16S rDNA sequence placed strain S2V2 in the genus Pseudoalteromonas, but th...

The aim of this study was to purify, characterise and evaluate the in vitro activity of MC21-B, an antibiotic produced by the marine bacterium Pseudoalteromonas phenolica O-BC30(T). MC21-B was purified by sequential silica and Cosmosil chromatography followed by high-performance liquid chromatography (HPLC). The chemical structure of MC21-B was det...

In this study, the extract was tested for in vitro activity against oxytetracycline-resistant V. harveyi . Toxicity of the methanolic extract was evaluated by Brain Shrimp Lethality test. Geodia sp. was characterized by three spicula types (oxeas, trianes and oxyaster euaster), encrusting growth formation, hispid surface features and skeletal struc...

The seasonal variations of nitrogen and phosphorus in bottom sediments of tidal flat and shallow water areas in the interior parts of the Ariake Sea were investigated during August, 2007-July, 2008. The supply amount of organic matter to sea bottom and sediment temperature had important effects on the seasonal variations of NH4-N in porewater. NO3-...

The aim of study was to know the effectiveness of garlic (Allium sativum) extract in preventing and curing Motile Aeromonas Septicemia (MAS) in common carp (Cyprinus carpio). Garlic extract was obtained by juicing fresh garlic and filtering by using filter paper. Common carp (8-12 cm in total lenght) were used. Infection was done by injecting intra...

Cat fish (Clarias sp.) is a favorite fish in Indonesia as this this fish has relatively cheap market price, and tolerant in intensive and high stocking density aquaculture system. However, this catfish is high susceptible to several diseases especially Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophyla. The application of immunostimu...

This research was aimed to investigate the pathogenicity of Vibrio fluvialis 24SK in humpback grouper (Cromileptes altivelis) based on its Lethal Dosage 50 (LD50). V. fluvialis 24SK was isolated from ren of humpback grouper cultured in floating net cage at Brackishwater Aquaculture Development Center (BADC) Situbondo, with vibriosis signs. The bact...

This research was conducted to isolate, identify, and characterize pathogenic Vibrio spp., causative agents of vibriosis in grouper at Brackishwater Aquaculture Development Center (BADC), Situbondo. Twenty-nine isolates were isolated from wound, gills, eyes, liver, and ren of grouper with vibriosis on Thiosulphate Citrate Bile Sucrose Agar (TCBSA)...

The objective of this research was to know the effectiveness of polyvalen Vibrio vaccine to control vibriosis in humpback grouper (Cromileptes altivelis). The effectiveness of vaccine was evaluated by the survival rate (SR), relative percent survival (RPS), mean time to death (MTD) as well as growth rate of vaccinated fish. This research consisted...

The objective of this paper was to compile and review the results of studies on probiotics and their applications especially for biological control agents in aquaculture. Antagonistic property of microorganisms against pathogenic microorganisms is a beneficial property to discover new microorganisms as candidates of biological control agents. Mecha...

Parasites often cause the great loss in aquaculture either in fish or shrimp aquacultures. The loss will become more serious when fish or shrimp are secondary infected especially by bacteria. The objective of this study was to identify parasites in fish and shrimp culture inYogyakartaSpecialProvince (DIY). Samples of fish and shrimp were collected...

Antibacterial activity of 2,4-diacetylphloroglucinol (DAPG) was evaluated against 23 vancomycin-resistant Staphylococcus aureus (VRSA) strains isolated from several Asian and European countries, Brazil, South Africa and USA, and against vancomycin-resistant Enterococcus spp (VRE) genotypes A, B and C. DAPG was active against a wide range of VRSA is...

S ince the first isolation of low-level vancomycin-resistant S. aureus (L-VRSA, or VISA) strain Mu50, there have been several L-VRSA strains with a similar level of vancomycin resistance reported from USA, South Africa and Brazil. The subsequent worldwide isolation of L-VRSA has confirmed that emergence of vancomycin resistance in S. aureus is a gl...

Four strains of aerobic, Gram-negative rods, motile by means of a single polar flagellum, that produced phenolic anti-methicillin-resistant Staphylococcus aureus (MRSA) substances and brown-pigmented colonies, were isolated from sea water. The G + C content of the DNA ranged from 39.9 to 40.6 mol%. The isolates grew at 18-37 degrees C and pH 6.5-9....

Previously 2,4-diacetylphloroglucinol (DAPG) produced by Pseudomonas sp. AMSN isolated from a marine alga, had demonstrated a high level of anti-methicillin-resistant Staphylococcus aureus (MRSA) comparable with that of vancomycin. In this study, this substance had bacteriolytic activity against MRSA at 1 microg/ml as well as similar activity again...

We previously reported a new marine bacterium, Pseudoalteromonas phenolica sp. nov. O-BC30T, which produced a bactericidal antibiotic against methicillin-resistant Staphylococcus aureus (MRSA). In the present study, we purified an anti-MRSA substance (MC21-A) from the methanol extract of the cells of P. phenolica O-BC30T and analyzed its chemical s...

Motile Aeromonas Septicimia is a dangerous disease on fresh water fish especially walking catfish and carp. Vaccination against the disease has been developed in Indonesia. However, there were high result variations of the vaccination. Immune response is influenced by level of vitamin C dietery. Objective of this experiment was to know effect of vi...