Alicia D. Carrera

Alicia D. Carrera
Universidad Nacional del Sur | UNS · Departamento de Agronomía

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39
Publications
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Publications

Publications (39)
Conference Paper
Full-text available
a) Race determination of P. halstedii isolates Fig. 1. A= recovery of inoculum at field B= detail of leaf sporulation C= spore solution D= reinfection of differential set of sunflower inbred lines E= phenotyping and race determination. b) SSR analysis The isolates were analyzed by eight loci SSR. The number of alleles per locus (APL), the expected...
Article
Full-text available
Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spr...
Article
The selection of fast-growing and high-yield-producing strains is required to satisfy the market demand on fungal food supplements. To that aim, three strains deposited in our collection as G. lucidum and G. oregonense were screened for polysaccharide production and biomass yield. Ganoderma strains deposited as G. lucidum were identified as G. sess...
Article
Full-text available
Qfhs.ndsu-3AS in wild emmer wheat (Triticum turgidum L. ssp. dicoccoides) is a major quantitative trait locus associated with type II resistance to Fusarium head blight (FHB). It was mapped in a population obtained from durum wheat Langdon (Triticum turgidum L. ssp. durum) and disomic Langdon-T. dicoccoides 3A substitution lines. Langdon(Dic-3A)-10...
Article
The objective of this work was to find practical procedures to overcome methodological drawbacks encountered during studies on sunflower downy mildew. Techniques for recovering living isolates of Plasmopara halstedii from the field and for the preservation of infected leaf samples for further molecular analysis were developed. A Polymerase Chain Re...
Article
Full-text available
Understanding the genetic basis of cold tolerance is a key step towards obtaining new and improved crop varieties. Current geographical distribution of durum wheat in Argentina exposes the plants to frost damage when spikes have already emerged. Biochemical pathways involved in cold tolerance are known to be early activated at above freezing temper...
Article
Full-text available
Fatty acid desaturases (FADs) catalyze the introduction of a double bond into acyl chains. Two FAD groups have been identified in plants: acyl-acyl carrier proteins (ACPs) and acyl-lipid or membrane-bound FAD. The former catalyze the conversion of 18:0 to 18:1 and to date have only been identified in plants. The latter are found in eukaryotes and b...
Article
Langdon(Dic-3A)-10 line carrying the QTL Qfhs.ndsu-3AS from T.Turgidum ssp. dicoccoides that confers Type II resistance to Fusarium head blight (FHB) was crossed with Argentinean durum wheat cultivars. F4 progeny were screened with the microsatellite locus Xgwm2, tightly linked to the Qfhs.ndsu-3A region. Reaction of these plants and parents to FHB...
Article
Panicum coloratum var. makarikariense, a perennial grass native to Africa, is adapted to a wide range of soil and climatic conditions with potential to be used as forage in tropical and semi-arid regions around the world. Our objective was to understand how the pollination mode affects viable seed production and further survival of the progeny. We...
Poster
Full-text available
Desde 2013 se observa un incremento de mildiu de girasol por Plasmopara halstedii en SE de Bs As y N de Santa Fé. Esto se atribuye a cambios genéticos del patógeno que confirieron tolerancia al curasemilla metalaxyl y/o superaron algunos genes Pl de resistencia de los híbridos cultivados. Ante este problema, se ha conformado una red compuesta por e...
Conference Paper
Full-text available
La introgresión de genes del cultivo en poblaciones silvestres emparentadas puede acelerar la adaptación de estas a nuevos ambientes, como los agro-ecosistemas. En Argentina, las poblaciones silvestres de girasol se comportan como ruderales y en algunos pocos casos, como malezas de los cultivos. El objetivo del trabajo fue evaluar el efecto de la i...
Article
The purpose of this research was to study the effect of vernalization on flowering time in thirty accessions of Triticum turgidum spp. durum. To this end, screening of allelic variation at VRN-1 loci was carried out using PCR-based markers and DNA sequencing, and gene expression analysis was performed in two and four developmental stages, with and...
Article
Full-text available
Summary. Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe, is a wheat disease that causes yield losses and mycotoxin accumulation in the grain. In germplasm of durum wheat (Triticum turgidum L. ssp. durum), genetic variability for resistance is scarce. To develop an improved assay for FHB resistance in wheat, we evaluated the effe...
Article
Panicum coloratum var. makarikariense is a perennial C4 grass native to South Africa with relatively good forage production under limited-resource conditions. Genetic characterisation and breeding efforts have been scant, thus limiting its use in cattle raising systems. The goal of the present study was to assess the genetic diversity of a collecti...
Article
The wheat recombinant chromosome inbred line LDN(Dic-3A)10, obtained through introgression of a Triticum dicoccoides disomic chromosome 3A fragment into Triticum turgidum spp. durum var. Langdon, is resistant to fusarium head blight (FHB) caused by Fusarium graminearum. To identify genes involved in FHB resistance, we used a cDNA-AFLP approach to c...
Article
Male sterile (MS) plants fail to produce functional anthers, pollen, or male gametes. This condition is useful in sunflower hybrid seed production. A MS form identified in an invasive population of H. annuus located in Las Malvinas was characterized by 37 morphological variables, six mitochondrial genes (atpA, atp6, coxIII, orfb, orfB and orfC) ass...
Article
Full-text available
Panicum coloratum (Poaceae) is a perennial grass native to South Africa, distributed all over the world, but poorly adopted by ranchers in Argentina. Adapted to a wide range of soil and climatic conditions, it constitutes a good forage resource for zones with edaphic and climatic restrictions, where cattle rising activities are here displaced. The...
Article
Full-text available
Genus Helianthus comprises diploid and polyploid species. An autoallopolyploid origin has been proposed for hexaploid species but the genomic relationships remain unclear. Mitotic and meiotic studies in annual Helianthus annuus (2n = 2x = 34) and perennial Helianthus resinosus (2n = 6x = 102) as well as the F-1 hybrids between both species were car...
Data
Full-text available
The concentration of yellow carotenoid pigments in durum wheat grain is an important quality criterion and is determined both by their accumulation and by their degradation by lipoxygenase enzymes (Lpx loci). The existence of a duplication at the Lpx-B1 locus and the allelic variation for a deletion of the Lpx-B1.1 copy is reported. This deletion w...
Article
Full-text available
The aim of this work was to map quantitative trait loci (QTLs) associated with flour yellow color (Fb*) and yellow pigment content (YPC) in durum wheat (Triticum turgidum L. var. durum). Additionally, QTLs affecting flour redness (Fa*) and brightness (FL*) color parameters were investigated. A population of 93 RILs (UC1113 × Kofa) was evaluated in...
Data
Fig. S1 QTLs mapping of flour brightness (FL*), flour redness (Fa*), flour yellow color (Fb*) and yellow pigment content (YPC) on chromosomes 1B, 5A and 5B in the linkage map of the UC1113 × Kofa RIL population. The centromere is indicated by a grey square. Markers are indicated to the right and their corresponding cumulative genetic distances are...
Article
Wild sunflower Helianthus annuus originates from North America and has naturalised in Argentina where it is considered invasive. The present study attempts to assess the genetic diversity using two different molecular marker systems to study the wild genetic patterns and to provide data applicable to conservation and breeding uses. Ten natural popu...
Article
Full-text available
Weeping lovegrass is well adapted for forage production and useful for soil conservation in semiarid regions, constituting a morphologically diverse group. Diploid genotypes are unfrequent and reproduce sexually, whereas the tetraploids and plants of higher ploidy levels reproduce by apomixis. In the present work RAPD, AFLP and EST-SSR were used in...
Article
A bright yellow color is an important quality criterion for pasta making. Yellow color depends on the amount of carotenoid pigments in grain, which is the result of the balance between pigment synthesis and degradation by lipoxygenases (LPX). The organization of genes coding for lipoxygenases in the tetraploid wheat genome is not completely underst...
Article
Helianthus petiolaris (Asteraceae) native to North America has naturalized in Argentina. The extensive overlapping with sunflower crop regions, the coincidence of life cycles and the common pollinators facilitate interspecific crosses with sunflower, H. annuus var. macrocarpus. To estimate the occurrence of crop-to-wild and wild-to-crop gene flow,...
Article
Full-text available
Two introduced wild species Helianthus annuus L. and H.petiolaris Nutt. have become widespread in central Argentina and overlap the sunflower crop region. Intermediate off-type plants between the wild and cultivated species are often found, which is of concern because of the recent release of imidazolinone resistant varieties and the likely use of...
Article
Full-text available
The aims of this work were (1) the saturation of a linkage map of durum wheat using AFPLs and RAPDs, (2) mapping of QTL related to lipoxygenase activity and (3) estimation of its usefulness in marker-assisted selection to increase pasta colour. A mapping population of 83 recombinant lines (RILs) derived from the cross between durum wheat Kofa and U...
Article
Naturalized populations of wild sunflower, Helianthus annnus, occur in six provinces of central Argentina, usually near sunflower crops. Plants of intermediate morphology indicate that gene flow might take place in both senses. In order to quantify gene flow between cultivated and wild sunflower, an experimental stand of sunflower was sown surround...
Article
Full-text available
Since 2000 a research is in progress on wild sunflowers in Argentina, comprising naturalized H. annuus and H. petiolaris populations, and their relationship with cultivated sunflower. The study includes morphological, phe-nological, biochemical and molecular aspects, addressed to explain dispersal and adaptive processes and gene flow within sunflow...
Article
Full-text available
Two wild Helianthus species native to North America have been natural-ized in Argentina, H. annuus ssp. annuus and H. petiolaris. They grow as adventitious overlapping about 50% of the crop area. Hybridization and intro-gression between these wild species and sunflower have important biological and practical consequences, the former including homop...
Article
Full-text available
Eight isozyme systems were used in this study: acid phosphatase (ACP), alcohol dehydrogenase (ADH), esterase (EST), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), 6-phosphogluconate dehydrogenase (PGD), and phosphoglucomutase (PGM). The polymorphism of these enzyme systems was studied in 25 elite inbred line...
Article
Full-text available
Dos especies de girasol silvestre originarias de América del Norte se han naturalizado en la Argentina: Helianthus annuus L. y H. petiolaris Nutt., anuales, diploides (x=17) y autoincompatibles. La primera no ha sido descripta anteriormente en el país y correspondería a H. annuus ssp. annuus. Se encontraron poblaciones establecidas en las provincia...
Article
Full-text available
Native populations of perennial grasses subjected to heavy grazing are typically shorter and more prostrate than ungrazed or lightly defoliated ones. However, it is often difficult to find out whether the morphological modifications are the result of genetic differentiation or phenotypic plasticity. Piptochaetium napostaense (Speg.) Hack. is a nati...
Article
Helianthus petiolaris is a wild species used in genetic improvement of sunflower, as a donor of cytoplasmic male sterility and of genetic resistance to diseases. Isozyme variation for ADH, ACP, EST, GDH, LAP, PGI, PGD and SKDH in this species was studied using starch gel electrophoresis. The patterns thus obtained were compared with zymograms of in...
Conference Paper
Fusarium head blight (FHB), caused by Fusarium graminearum, is a wheat disease that leads to economic losses due to reduced grain yield and quality together with rejection or downgrading of grain at marketing due to contamination by mycotoxins. Environmental temperature and humidity during flowering affect the onset of the infection. Resistance to...

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Projects

Projects (3)
Project
QTL mapping in biparental populations
Archived project
El proyecto se orienta a colectar, clasificar y valorizar como recurso fitogenético a las poblaciones naturales de los géneros Helianthus, Brassica, Vicia y Sorghum que se han naturalizado e integran la flora de nuestro país. Los trabajos previos del grupo muestran que existe una notoria biodiversidad que no ha sido totalmente relevada. Las poblaciones serán localizadas y se colectarán sus propágulos. Se establecerá la asociación entre parámetros edáficos, climáticos y de la biota acompañante con la presencia de las poblaciones naturales. Se colectarán individuos con indicios de cruzamientos espontáneos con los cultivos congéneres. El material colectado será observado en un jardín común para cada género y se establecerá la existencia de biotipos diferentes mediante rasgos fenotípicos y moleculares. Los rasgos que resulten de interés serán incrementados en poblaciones pre-seleccionadas que constituirán stocks genéticos que se depositarán en los bancos de germoplasma del país y se pondrán a disposición de los programas de mejoramiento de las instituciones estatales. Como producto del proyecto se apunta a lograr que la totalidad de la biodiversidad actual de los géneros estudiados se mantenga en condiciones que aseguren su disponibilidad para la mejora de los cultivos emparentados actual o futura.
Project
To know the genetics of the current races of the pathogen in Argentina. Do a survey of the curent pathotypes and a molecular characterization using tools like sequencing.