Ali Zohaib

Ali Zohaib
The Islamia University of Bahawalpur | IUB · Department of Microbiology

DVM, MPVM and PhD

About

41
Publications
35,647
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
906
Citations
Introduction
Virology; Human-animal interface; Global pathogen surveillance; Preparedness and response; Host-pathogen interaction
Additional affiliations
September 2012 - July 2015
Huazhong Agricultural University
Position
  • Master's Student
September 2007 - July 2012
University of Agriculture Faisalabad
Position
  • Doctor of Veterinary Medicine

Publications

Publications (41)
Article
Full-text available
SUMMARY This study describes the first large-scale serosurvey on West Nile virus (WNV) conducted in the equine population in Pakistan. Sera were collected from 449 equids from two provinces of Pakistan during 2012-2013. Equine serum samples were screened using a commercial ELISA kit detecting antibodies against WNV and related flaviviruses. ELISA-p...
Article
Full-text available
The innate immune system provides protection against invading neurotropic viruses. It acts as the first line of defense against invading viruses and plays an elementary role in their pathogenesis. The list of viruses capable of infecting human central nervous system (CNS) is quite long, most important of them are Japanese Encephalitis virus (JEV),...
Article
Full-text available
A new hepatitis E virus (HEV-7) was recently found in dromedaries and 1 human from the United Arab Emirates. We screened 2,438 dromedary samples from Pakistan, the United Arab Emirates, and 4 African countries. HEV-7 is long established, diversified and geographically widespread. Dromedaries may constitute a neglected source of zoonotic HEV infecti...
Article
Full-text available
Dromedary camels from Africa and Arabia are an established source for zoonotic Middle East respiratory syndrome coronavirus (MERS-CoV) infection among humans. In Pakistan, we found specific neutralizing antibodies in samples from 39.5% of 565 dromedaries, documenting significant expansion of the enzootic range of MERS-CoV to Asia.
Article
Full-text available
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in Pakistan, nasal (n = 776) and serum (n = 1050) samples...
Article
Full-text available
The present study evaluated the prevalence of fluoroquinolone resistance and the underlying mechanisms among Salmonella Typhi clinical strains from Punjab, Pakistan. A total of 174 Salmonella Typhi strains were isolated from the blood culture samples. The strains identification was done by using the API 20E system and VITEK®, while serovar validati...
Article
Full-text available
Ubiquitination, also denoted ubiquitylation, is a posttranslational modification that has been implicated in the regulation of both innate and adaptive immune responses. Ubiquitination plays crucial roles in innate immune signaling by ensuring the proper orchestration of several signaling mediators that constitute a functional immune response. Here...
Article
Full-text available
Here, we report the coding-complete genome sequence of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolate obtained from a nasopharyngeal swab from the first patient with COVID-19 in Gilgit, Pakistan.
Article
Full-text available
The emergence of SARS-CoV-2 has been reported during December 2019, in the city of Wuhan, China. The transmission of this virus via human to human interaction has already been described. The novel virus has become pandemic and declared as a comprehensive emergency worldwide by World Health Organization due to its exponential spread within and outsi...
Article
Full-text available
Background: Coronavirus disease 2019 (COVID-19) linked with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cause severe illness and life-threatening pneumonia in humans. The current COVID-19 pandemic demands an effective vaccine to acquire protection against the infection. Therefore, the present study was aimed to design a multiepitop...
Article
Full-text available
Brucellosis is an important zoonosis worldwide. Equines are susceptible to the infection when in close contact with infected animals. The objective of our study was to update the existing knowledge and detect and differentiate the causative agent of brucellosis in breeding equines in Punjab, Pakistan. A cross-sectional study was designed to evaluat...
Article
Full-text available
Brucella abortus and Brucella melitensis are important zoonotic pathogens. Brucellosis is an occupational hazard for veterinarians and animal handlers. The current work aimed to determine the prevalence of brucellosis in buffaloes and goats of Islamabad Capital Territory, Pakistan. A total of 341 milk samples from buffaloes (n=180) and goats (n=161...
Article
Full-text available
We describe the seroprevalence of severe fever with thrombocytopenia syndrome virus (SFTSV) and the association of antibody occurrence with location, sex, and age among the human population in Pakistan. Our results indicate substantial activity of SFTSV and SFTSVrelated viruses in this country.
Article
Full-text available
We detected Crimean-Congo hemorrhagic fever virus infections in 4 provinces of Pakistan during 2017–2018. Overall, seroprevalence was 2.7% in humans and 36.2% in domestic livestock. Antibody prevalence in humans was highest in rural areas, where increased contact with animals is likely
Article
An extended range of host susceptibility including camel has been evidenced for some of the important veterinary and public health pathogens, such as brucellosis, peste des petits ruminants (PPR) and bluetongue (BT). However, in disease endemic settings across many parts of the globe, most of the disease control interventions accounts for small and...
Preprint
Full-text available
During December 2019, a novel coronavirus named as 2019-nCoV, has emerged in Wuhan, China. The human to human transmission of this virus has also been established. Untill now the virus has infected more than seven thousand people and has spread to fifteen countries. The World Health Organization (WHO) has declared 2019-nCoV as global health emergen...
Preprint
Full-text available
Background: Coronavirus disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Corona virus 2 (SARS-COV-2) was first diagnosed in December 2019, Wuhan, China. Little is known about this new virus and it has the potential to cause severe illness and pneumonia in some people, therefore the development of an effective vaccine is highly de...
Article
Full-text available
Objectives: This study was performed to determine the presence of West Nile virus (WNV) in mosquito specimens and human blood donors from Pakistan. Methods: 4,500 mosquito specimens were collected using CO2-baited traps from five selected districts of Punjab province, Pakistan. Mosquito were taxonomically identified using standard morphological key...
Article
Full-text available
We describe the first genome isolation of Middle East respiratory syndrome coronavirus (MERS-CoV) in Kenya. This fatal zoonotic pathogen was first described in the Kingdom of Saudi Arabia in 2012. Epidemiological and molecular evidence revealed zoonotic transmission from camels to humans and between humans. Currently, MERS-CoV is classified by the...
Article
Full-text available
Many blood-feeding arthropods are known vectors of viruses that are a source of unprecedented global health concern. Mosquitoes are an integral part of these arthropod vectors. Advancements in next-generation sequencing and bioinformatics has expanded our knowledge on the richness of viruses harbored by arthropods. In the present study, we applied...
Article
Full-text available
This is the first country-wide surveillance of bat-borne viruses in Kenya spanning from 2012–2015 covering sites perceived to have medium to high level bat-human interaction. The objective of this surveillance study was to apply a non-invasive approach using fresh feces to detect viruses circulating within the diverse species of Kenyan bats. We scr...
Data
Univariate analysis of potential risk factors associated with Middle East respiratory syndrome coronavirus seropositivity in dromedary camels in Punjab, Pakistan.
Article
The type I interferon (IFN) response is part of the first-line defense against viral infection. To initiate replication, viruses have developed powerful evasion strategies to counteract host IFN responses. In the present study, we found that the Japanese encephalitis virus (JEV) NS5 protein could inhibit double-stranded RNA (dsRNA)-induced IFN-β ex...
Article
Full-text available
MicroRNAs (miRNAs) are small non-coding RNAs that play important roles in regulating the host immune response. Here we found that miR-22 is induced in glial cells upon stimulation with poly(I:C). Overexpression of miR-22 in the cultured cells resulted in decreased activity of interferon regulatory factor-3 and nuclear factor-kappa B, which in turn...
Article
Full-text available
Ubiquitination, also denoted ubiquitylation, is a posttranslational modification that has been implicated in the regulation of both innate and adaptive immune responses. Ubiquitination plays crucial roles in innate immune signaling by ensuring the proper orchestration of several signaling mediators that constitute a functional immune response. Here...
Data
Individual optical density ratios obtained from ELISA testing of serum and fecal samples from dromedary camels.
Article
Full-text available
Cancer is caused by a series of alterations in genome and epigenome mostly resulting in activation of oncogenes or inactivation of cancer suppressor genes. Genetic engineering has become pivotal in the treatment of cancer and other genetic diseases, especially the formerly-niche use of clustered regularly interspaced short palindromic repeats (CRIS...
Article
Full-text available
Importance: MiRNAs are critical regulators of gene expression that utilize sequence complementarity to bind to and modulate the stability or translation efficiency of target mRNAs. Accumulating data suggest that miRNAs regulate a wide variety of molecular mechanisms in the host cells during viral infections. JEV, a neurotropic flavivirus, is one o...
Article
Full-text available
Japanese encephalitis virus (JEV) can target CNS and cause neuroinflammation that is characterized by profound neuronal damage and concomitant microgliosis/astrogliosis. Although microRNAs (miRNAs) have emerged as a major regulatory network with profound effects on inflammatory response, it is less clear how they regulate JEV-induced inflammation....
Article
Full-text available
Toxoplasmosis is a serious threat for livestock in addition to being of zoonotic significance. In this study, serodiagnosis of equine toxoplasmosis was conducted in a randomly selected population from the 3 metropolises of Punjab, Pakistan. To this end, 272 draught equines were screened using a commercial latex agglutination assay kit. Association...
Article
Full-text available
Astrocyte-derived inflammation is a common component of acute or chronic injury in the central nervous system. MicroRNAs (miRNAs) are small non-coding RNAs that play important regulatory roles in the inflammatory response. In this study, we found that miR-206 is induced upon stimulation with lipopolysaccharide. Overexpression of miR-206 in astrocyt...
Article
Full-text available
Background: Japanese encephalitis virus (JEV) is a neurotropic flavivirus that causes Japanese encephalitis (JE), which leads to high fatality rates in human. Tumor necrosis factor alpha (TNF-α) is a key factor that mediates immunopathology in the central nervous system (CNS) during JE. Etanercept is a safe anti-TNF-α drug that has been commonly u...
Article
Full-text available
Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes the most prevalent viral encephalitis in Asia. The NS5 protein of JEV is a key component of the viral replicase complex, which plays a crucial role in viral pathogenesis. In this study, tandem affinity purification (TAP) followed by mass spectrometry analysis was performed...
Article
Full-text available
An unusual case of traumatic carditis in a Tedday goat is described. No significant other accompanying abnormalities were observed at necropsy except the presence of 4.5 mm long sharp quilt needle, which was extending from the left ventricle to right atrium.
Article
Japanese Encephalitis virus (JEV) is a mosquito borne flavivirus that infects macrophages, monocytes and dendritic cells (DCs) during in vivo replication. The C-type lectins DC-SIGN and DC-SIGNR have been reported to act as cell attachment factors for diverse array of pathogens. In this study, the effect of these lectins on JEV infection was invest...
Article
Full-text available
Key words: Calcium oxalate dihydrate Enrofloxacin Escherichia coli Struvite Urethral plug This report describes the lower urinary tract disease (LUTD) in four male cats with two different etiologies. All animals were under three years of age and on commercial dry diet. Treatment guidelines prescribed for obstructive and non-obstructive cases were f...

Questions

Questions (5)
Question
Hi
I would like to ask is it possible to do heat inactivation of serum samples without water bath. We are dealing with over 200 human serum samples and our water bath capacity is limited. Looking forward to hearing suggestions and personal experiences
Regards
Question
Hello Everyone
Hope you are doing well
Actually, I am new to MrBayes, Before analysis of my data i am trying to replicate data from my reference paper (already published) just to compare my results. However, I am getting some convergence issues Please see the attached three pictures. Although when I used the MEGA6 ML tree convergence was same as expected. My aim is just to analyse by using Model "nst=6 rates=invgamma" 
I used two different blocks , 1st time (block 1) give less convergence and the second time  (2n dblock) give more however still the issue of Branch NC006430 remains it should resemble with the reference paper. Both blocks were used separately. 
Block 1
begin mrbayes;
set autoclose=yes nowarn=yes;
lset
nucmodel=4by4
nst=6
code=Universal
ploidy=Diploid
rates=Invgamma
ngammacat=4
nbetacat=5
omegavar=Equal
covarion=No
coding=All
parsmodel=No;
mcmcp
filename=siMBaoutput
nruns=2
nchains=4
swapfreq=1
samplefreq=500
printfreq=500
burninfrac=0.25
temp=0.1
nswap=1
mcmcdiagn=Yes
diagnfreq=5000
diagnstat=Avgstddev
savetrees=No
minpartfreq=0.1
allchains=No
allcomps=No
relburnin=Yes
burnin=0
stoprule=No
stopval=0.05
ordertaxa=No
ngen=1000000;
mcmc;
sump;
sumt
contype=halfcompat
conformat=Figtree;
end;
Block 2
begin mrbayes;
set autoclose=yes nowarn=yes;
lset nst=6 rates=invgamma;
mcmc ngen=1000000 relburnin=yes burninfrac=0.25 samplefreq=1000 printfreq=10000 nchains=4 savebrlens=yes;
sump burnin=2500;
sumt burnin=2500;
END;
Can you please suggest me how should I resolve the issue. I will be highly obliged.
 Looking forward for your kind response. 

Network

Cited By

Projects

Projects (5)
Project
We will determine temperature thresholds that precipitate heat stress in Pteropus medius at roost level We will explore how the presence and distance of different landscape variables influences heat stress. We will see how weather patterns impact the thermoregulatory behaviors. We will generate models for other Pteropus medius roosting sites by using these studied variables. We will also conduct interviews of the local population to identify historic die offs and dietary ecology of Pteropus medius in Pakistan. This study also aims to determine the presence of pathogenic E. coli and Salmonella in free-ranging fruit bats at eight different roosts located in both urban and semi-urban setting of Pakistan. AMR of selected E.coli and Salmonella isolates will be performed. We will identify AMR genes in the fecal E. coli isolates. Additionally, this study also aims to develop and demonstrate a methodologically robust approach to determining and interpreting cortisol values in individual fecal samples collected from wild, free-living flying-fox populations. We will determine fecal bacterial load and its association with physiological stress by monitoring cortisol levels. We will describe population-level fecal cortisol range in the Indian flying foxes, and compare the fecal cortisol levels with various environmental, landscapes variables and fecal AMR resistance patterns.
Project
Computational analysis of infectious diseases and immunology
Project
To investigate Emerging Infectious Diseases under the umbrella of One Health in Pakistan