Ali A Moosavi-Movahedi

University of Tehran | UT · Institute of Biochemistry and Biophysics

Sturgeons are living fossils and among the oldest surviving vertebrate species, and Caspian Sea is habitat of the vast majority of sturgeon species. It is tempting to assume that speciation of sturgeons occurred in response to the severe environmental changes during the formation of Caspian Sea from the ancient Paratethys Sea. Hemoglobin (Hb) refle...

Laccases have been broadly applied as a multitasking biocatalyst in various industries, but their applications tend to be limited by easy deactivation, lack of adequate stability, and susceptibility under complex conditions. Identifying stable laccase as a green-biocatalyst is crucial for developing cost-effective biorefining processes. In this dir...

در برخی از کشورهای جهان پیشینه ایجاد شبکه آزمایشگاه های ملی ماموریت گرا و پیشرو در قلمرو های کلیدی که محل تلاقی علم و فناوری و جایگاه استقرارامکانات بزرگ پژوهشی و فعالیت دانشمندان و مهندسان برجسته می باشد به بیش از نیم قرن می رسد. چنین آزمایشگاه هایی هم افزایی قابل توجهی با دانشگاه ها در گسترش مرزهای علوم فاخر و فناوری های نافذ دارند. امکانات بزرگ...

In today's world, many problems, such as the deadly pandemics that science seeks to solve, are borderless in nature, and overcoming such challenges goes through the international scientific cooperation between scientists and policymakers working across geopolitical boundaries. Over the past few decades, in parallel with traditional diplomacy, a new...

Quinoa (Chenopodium quinoa Willd) is a potential source of protein with ideal amino acid profiles which its bioactive compounds can be improved during germination and gastrointestinal digestion. The present investigation studies the impact of germination for 24 hr and simulated gastrointestinal digestion on α-glucosidase inhibitory activity of the...

Oxidative stress is the leading player in the onset and development of various diseases. The Keap1-Nrf2 pathway is a pivotal antioxidant system that preserves the cells’ redox balance. It decreases inflammation in which the nuclear trans-localization of Nrf2 as a transcription factor promotes various antioxidant responses in cells. Through some oth...

The health effects of ambient air particulate matter with a diameter of ≤2.5 μm (PM2.5) on the central nervous system are well known and the induced oxidative stress has been shown as their main neuropathologic outcome. Ambient air PM2.5 sampling methods mostly use air sampler systems that collect PM2.5 on filters, which is followed by a PM2.5 extr...

There have been recent renewed interests in the importance of suicide enzyme inhibition. The principal objective of this review is to investigate all types of suicide inhibitions for natural enzymes, artificial biocatalysts as well as therapeutic potential of enzyme suicide inhibition. It is discussed the suicide inhibition beneficial in drug desig...

Unlike cytosolic proteins, membrane proteins (MPs) are embedded within the plasma membrane and the lipid bilayer of intracellular organelles. MPs serve in various cellular processes and account for over 65% of the current drug targets. The development of membrane mimetic systems such as bicelles, short synthetic polymers or amphipols, and membrane...

Herein, an innovative, green, and practical biocatalyst was developed using conjugation of a novel bifunctional mannanase/xylanase biocatalyst (PersiManXyn1) to the modified cellulose nanocrystals (CNCs). Firstly, PersiManXyn1 was multi-stage in-silico screened from rumen macrobiota, and then cloned, expressed, and purified. Next, CNCs were synthes...

Nowadays, there is an ever-increasing trend in the case of nutraceuticals and superfoods as a result of growing concerns about the effects of diet on health. Nutraceuticals are natural biologically active compounds extractable from various food sources. In contrast, a superfood is any fresh or processed food claimed to have particular health-promot...

Teduglutide is the only drug approved for long-term treatment of short bowel syndrome (SBS). This drug exerts its pharmacological effects via binding to the GLP-2 receptors (ECD-GLP2R) located in intestinal tissue. The three dimensional (3D) structure of ECD-GLP2R hasn't been determined yet and hence its mode of interaction with agonists/antagonist...

The scope of literacy influencing the quality of social life in a given society is to be defined where it is necessary that infrastructure required for its development be also outlined. Literacy in its broad context can be classified into three main categories: 1) General literacy and skills consisting of those that deal with reading, writing and n...

In this era, human life has been exposed to various dangers due to extensive environmental changes, population growth, obesity, unhealthy diets, sedentary lifestyles, unrest and tension which can be considered as the consequence of his destructive activities in many cases. The effect of these factors on human health occurs through interference with...

Degradation of various types of lignocellulosic biomass that require harsh pretreatment and abnormal operating conditions often occur in presence of a large amount of salt. Therefore, identification and use of stable and halotolerant cellulases is essential for industrial lignocellulose applications under extreme pH and temperature. This study focu...

Proteins have evolved in specific 3D structures and play different functions in cells and determine various reactions and pathways. The newly synthesized amino acid chains once depart ribosome must crumple into three-dimensional structures so can be biologically active. This process of protein that makes a functional molecule is called protein fold...

2-hydroxy-1, 4-naphthoquinone is a color phyto-chemical component taken from Henna. It can make an impact on hemoglobin structure certainly change hemoglobin functions. In some of in vivo study formation of hemichrome and Heinz body has been seen and these effects will lead to kidney, spleen and liver toxicity by increase their weight, iron sedimen...

The conversion of soluble proteins into amyloid fibrils has importance in protein chemistry, biology, biotechnology and medicine. A novel lipase from Pseudomonas sp. was previously shown to have an extremely high aggregation propensity. It was therefore herein studied to elucidate the physicochemical and structural determinants of this extreme beha...

This book argues that, to be healthy, human beings should love nature and stay in balance with it as much as possible. In other words: do not unbalance nature so that your own balance is not disturbed. The best and healthiest way for human beings to live is to find balance in life and nature. In this regard, the book discusses useful, nutritious, f...

Technologies strongly influence lifestyle behaviors; therefore, knowledge, rationality, and behavior are more important in healthy living. Today, many artificial technologies are abnormal and create pollutants that produce stress (unbalanced free radicals) for humans and creature. Molecular stress generates a variety of unbalanced free radicals fro...

Background Type 2 diabetes mellitus (T2DM) is a challenging and globally ubiquitous metabolic disease caused by insulin resistance. Skeletal muscle is the major insulin-sensitive tissue that plays a great role in blood sugar homeostasis. Dysfunction of muscle metabolism is implicated in the disturbance of glucose hemostasis and the development of i...

Background Type 2 diabetes mellitus (T2DM) is a challenging and globally ubiquitous metabolic disease caused by insulin resistance. Skeletal muscle is the major insulin-sensitive tissue that plays a great role in blood sugar homeostasis. Dysfunction of muscle metabolism is implicated in the disturbance of glucose hemostasis and the development of i...

Nuclear factor erythroid 2-related factor 2 (Nrf2) is an essential transcription factor that maintains the cell’s redox balance state and reduces inflammation in different adverse stresses. Under the oxidative stress, Nrf2 is separated from Kelch-like ECH-associated protein 1 (Keap1), which is a key sensor of oxidative stress, translocated to the n...

Background Type 2 diabetes mellitus (T2DM) is a challenging and globally ubiquitous metabolic disease caused by insulin resistance. Skeletal muscle is the major insulin-sensitive tissue that plays a great role in blood sugar homeostasis. Dysfunction of muscle metabolism is implicated in the disturbance of glucose hemostasis and the development of i...

Antimicrobial peptides (AMPs) are at the focus of attention due to their therapeutic importance and developing computational tools for the identification of efficient antibiotics from the primary structure. Here, we utilized the 13CNMR spectral of amino acids and clustered them into various groups. These clusters were used to build feature vectors...

Coeliac disease, or celiac sprue, is an autoimmune disorder of the small intestine with a genetic background. As a result of immunological responses to gluten consumption, small bowel villi will have damaged and cause malabsorption of nutrients. The symptoms of this disorder appeared due to presence of Gluten, a stored protein in the endosperm of g...

Background: Type 2 diabetes mellitus (T2DM) is a complex multifactorial disease with a high prevalence worldwide. Insulin resistance and impaired insulin secretion are the two major abnormalities in the pathogenesis of T2DM. Skeletal muscle is responsible for over 75% of the glucose uptake and plays a critical role in T2DM. Here, we sought to prov...

Insulin rapidly fibrillates in the presence of amyloid seeds from different sources. To address its cross-reactivity we chose the seeds of seven model proteins and peptides along with the seeds of insulin itself. Model candidates were selected/designed according to their size, amino acid sequence, and hydrophobicity. We found while some seeds provi...

Creation has a high spirituality and human is the abstract of the universe and has dignity of creation. Human health is the result of maintaining a balance between human nature with that of external state. Any deviations from these natural states lead to an unbalance condition in human life resulting to unhealthy consequences. Therefore, understand...

In this report we highlight the crucial importance of advanced and cutting edge basics and fundamental research in today in rapid advancement of technology. Technology production in the context of consumer goods such as smart mobile phone has extraordinary impacts on the daily life of every human being. This report attempts to negate the belief tha...

Curcumin, a natural compound derived from turmeric, has antioxidant, anti-tumor and anti-inflammatory activities. Accumulated evidence indicated curcumin plays an inhibitory role against infection of numerous viruses. Curcumin exerts its antiviral activity on various viruses through different mechanisms. These mechanisms involve either a direct int...

While polysaccharide-based superabsorbent hydrogels (SHs) have attracted increasing interest as proficient carriers in the enzyme immobilization, the nature of the favored interactions between the SHs and enzymes is still unclear. Herein, a combined experimental and computational study was employed to investigate the dominant parameters affecting o...

Background Nowadays, the biological properties and anticancer activities of platinum-based drugs and metal coordination complexes have received particular attention. These compounds have revealed the clinical potential in cancer chemotherapy. Objective In this research, the anticancer activity and molecular simulation of two newly synthesized binu...

Recently, nanoencapsulation was introduced as an efficient and promising approach for the protection, delivery, and site-specific liberation of the nutraceuticals and bioactive ingredients. Food proteins are attractive materials for developing nanocarriers to protect and deliver bioactives due to their unique functional and biological properties. F...

In this era, human life has been exposed to various dangers due to extensive environmental changes, population growth, obesity, unhealthy diets, sedentary lifestyles, unrest and tension which can be considered as the consequence of his destructive activities in many cases. The effect of these factors on human health occurs through interference with...

This paper studies the effects of electromagnetic fields (EMF), specifically with circular electric field polarization, on the structure of Hemoglobin. Nearly all of the previous studies are based on linearly-polarized waves. But our comparative study based on a novel dual-polarized waveguide exposure device, shows that the EMF effects on macromole...

The walnut proteins were trypsin-hydrolyzed for 0, 15, 30, 60 and 120 min and the effects of hydrolysis on the structural, functional, antioxidant, bioactive-loading, and angiotensin converting enzyme (ACE)-inhibitory properties of walnut protein were studied. The trypsin-mediated hydrolysis of walnut proteins was monitored using sodium dodecyl sul...

As an immediate priority, finding a solution for the new coronavirus (SARS-CoV-2) pandemic is on the agenda of scientists of the world. Herein, we summarize all the suggested global solutions proposed by scientists and physicians so far to stop this dangerous virus. Also, the important molecular targets for hitting the killer virus are highlighted.

In this study, walnut protein was employed as a novel carrier for the encapsulation of curcumin as a bioactive molecule using the solvent-free basic pH-shifting method. The encapsulation parameters (encapsulation efficiency and loading amount), the structural properties, reducing power, free radical (ABTS and DPPH) scavenging activity, and in vitro...

Background: Type 2 diabetes mellitus (T2DM) is a complex multifactorial disease with a high prevalence in the world. Insulin resistance and impaired insulin secretion are the two major abnormalities in the pathogenesis of T2DM. Skeletal muscle is responsible for over 75% of the glucose uptake thus plays a critical role in T2DM. Here, we attempt to...

Whey protein isolate (WPI) was first treated with citric acid as a cross-linker to yield whey protein aggregates (WPA). Formation of aggregates through the covalent cross-linking was confirmed by SDS-PAGE. After that, with curcumin as a bioactive cargo, the potential application of WPA as carriers was investigated. WPA showed a remarkable higher lo...

In this study, we focused on the interference of methylparaben (MP), as the most commonly used paraben, on hemoglobin (Hb) fructation, generating reactive oxygen species (ROS) and resulting lipid peroxidation (LPO). We showed the enhancement of ROS generation and heme degradation during hemoglobin fructation in the presence of MP using some biophys...

Background Type 2 diabetes mellitus (T2DM) is a complex multifactorial disease with a high prevalence in the world. Insulin resistance and impaired insulin secretion are the two major abnormalities in the pathogenesis of T2DM. Skeletal muscle is responsible for over 75% of the glucose uptake thus plays a critical role in T2DM. Here, we attempt to p...

Protein aggregation to form amyloid is associated with many human diseases, increasing the need to develop inhibitors of this process. Here we evaluate the ability of derivatives of the small organic compound noscapine, derived from the opium poppy, to inhibit fibrillation of the model protein insulin. We combined biophysical methods to assess insu...

Background: Type 2 diabetes mellitus (T2DM) is a complex multifactorial disease with a high prevalence in the world. Insulin resistance and impaired insulin secretion are the two major abnormalities in the pathogenesis of T2DM. Skeletal muscle is responsible for over 75% of the glucose uptake, thus plays a critical role in T2DM. Here, we attempt to...

Background: Type 2 diabetes mellitus (T2DM) is a complex multifactorial disease with a high prevalence in the world. Insulin resistance and impaired insulin secretion are the two major abnormalities in the pathogenesis of T2DM. Skeletal muscle is responsible for over 75% of the glucose uptake, thus plays a critical role in T2DM. Here, we attempted...

Background Type 2 diabetes mellitus (T2DM) is a complex multifactorial disease with a high prevalence in the world. Insulin resistance and impaired insulin secretion are the two major abnormalities in the pathogenesis of T2DM. Skeletal muscle is responsible for over 75% of the glucose uptake, thus plays a critical role in T2DM. Here, we attempted t...

Background Type 2 diabetes mellitus (T2DM) is a complex multifactorial disease with a high prevalence in the world. Insulin resistance and impaired insulin secretion are the two major abnormalities in the pathogenesis of T2DM. Skeletal muscle is responsible for over 75% of the glucose uptake, thus plays a critical role in T2DM. Here, we attempted t...

Background Type 2 diabetes mellitus (T2DM) is a complex multifactorial disease with a high prevalence worldwide. Insulin resistance and impaired insulin secretion are the two major abnormalities in the pathogenesis of T2DM. Skeletal muscle is responsible for over 75% of the glucose uptake and plays a critical role in T2DM. Here, we sought to provid...

L-Arginine (Arg or R) is a non-toxigenic, metabolically versatile and conditionally essential amino acid. Single-pot synthesis of Arg modified magnetic nanoparticles (RMNPs) was achieved using magnetite [Fe3O4] nanoparticles (MNPs) and binary function of Arg as a functional group and an alkali precipitator. This modification is supposed to offer se...

In this research, gum Arabic (GA) and whey protein nanofibrils (WPN) were employed for the encapsulation of curcumin as a bioactive compound with low water solubility through the complex coacervation method. The optimum conditions for the formation of complex coacervates were found at WPN/GA weight ratio of 1:1 and pH value of 3.0. The resulting co...

The α-Crystallin (α-Cry) functions as a molecular chaperone, preventing the formation of stress-induced protein aggregation which is important for maintenance of lens transparency. The kinetic data of Wt, R69C and D109H αB-Crys chaperone-like activity were obtained by UV-Vis spectroscopy in both thermal- and chemical-induced aggregation methods. Th...

L-Arginine (Arg or R) is a non-toxigenic, metabolically versatile and conditionally essential amino acid. Single-pot synthesis of Arg modified magnetic nanoparticles (RMNPs) was achieved using magnetite [Fe3O4] nanoparticles (MNPs) and binary function of Arg as a functional group and an alkali precipitator. This modification is supposed to offer se...

In the current study, the foaming properties of native egg white proteins (EWP) were compared to those of fibrillated EWP formed by the heating of EWP solution at acidic condition. Transmission electron microscopy (TEM) revealed that different fibrillated morphologies were formed dependent on heating duration. In general, foams produced from fibril...

In the present paper the effects of 940 MHz electromagnetic field (EMF) with two types of polarization (linear vs. circular electric field polarization) on the structure of Adult Hemoglobin (HbA) protein macromolecule are studied. The waves produced by mobile phone bases stations in urban areas are almost circularly-polarized, whereas most conventi...

Ellagic acid (ELA), as a polyphenolic natural compound and food additive, which has reported to possess anti-carcinogenic, antioxidant, antidepressant, ameliorative and anti-mutagenic effects. In the current work, the effects of ELA on the conformation and catalytic activity of catalase were investigated by using spectroscopic techniques including...

Stress-induced unfolding and fibrillation of insulin represent serious medical and biotechnological problems. Despite many attempts to elucidate the molecular mechanisms of insulin fibrillation, there is no general agreement on how this process takes place. Several previous studies suggested the importance of the C-terminal region of B-chain in thi...

Cold gelation of egg white proteins (EWP) has still remained a challenge because they suffer from heat-induced gelation at low protein concentrations during the preheating step. This study suggested that adjusting pH of EWP solution at relatively high alkaline pH (i.e. 11.3) could inhibit the heat-induced gelation of EWP, allowing to produce therma...

One of the important physicochemical features of the proteins specifically multi-subunit types is their stability at high temperatures. The kinetics of the dissociation and denaturation of proteins possessing at least two subunits has certain challenges because the overall mechanism of dissociation can include hidden reversible and/or irreversible...

Background: Diabetes is a chronic disease associated with many problems and high costs. In recent decades, a lot of research has been carried out in order to improve methods of treatment of diabetic patients. One of the currently used complementary therapies for diabetes is ozone therapy or autohemotherapy. The beneficial effects of ozone has been...

Enzyme immobilization onto supports could improve its specificity, storage stability, reusability, pH tolerance and thermal stability. Aiming to provide a proficient immobilization carrier for a recombinant xylanase (PersiXyn1), the high-performance 3D copolymeric network of carboxymethyl cellulose (CMC)-based hydrogel sponges were synthesized usin...