Ali Karami

Baqiyatallah University of Medical Sciences | BMSU · Molecular Biology www.dralikarami.com

Molecular biology plays an important role in understanding the structures, functions and internal controls within each cell. All this can be used to diagnose the diseases, effectively target new medicines and better understand cellular physiology. The bacteria that cause Lyme disease are difficult to observe directly in body tissues and too time-co...

ناشر: دانش بنیاد مولف: استیون ام. دروکر مترجم: سیده مأنوس وطن خواه زیر نظر: پروفسور علی کرمی (استاد تمام دانشگاه علوم پزشکی بقیه الله) حقایق پشت پرده کمپانی های قدرتمند تولید کننده بذرهای تراریخته و نفوذ در دولت آمریکا برای کنترل غذای جهان فعالیت می کنند کتابی مستند با 1150 منبع معتبر علمی و افشای اسناد از درون کمپانی های تراریخته مثل مونسانتو تو...

Increasing private car ownership and car dependency has led to a low share of walking as an active mode as well as congestion, air pollution, and health problems in developing countries. This paper aims to identify and compare the impacts of a selection of socio-economic, travel-related, and built environment variables on walking likelihood and res...

کتاب ژن‌های تغییریافته، حقیقتِ تحریف‌شده ترجمۀ سیده مأنوس وطن‌خواه زیر نظر پروفسور علی کرمی از انتشارات دانش‌بنیاد یکی از مستندترین کتاب‌های نوشته‌شده در موضوع تراریخته‌ها با بیش از 1150 منبع علمی و در واقع یکی از بهترین کتاب‌های پنجاه سال اخیر جهان است که مورد تحسین دانشمندان قرار گرفته است. در این کتاب به چگونگی ایجاد بذرهای تراریخته و کنترل...

کتاب ژن‌های تغییریافته، حقیقتِ تحریف‌شده ترجمۀ سیده مأنوس وطن‌خواه زیر نظر پروفسور علی کرمی از انتشارات دانش‌بنیاد یکی از مستندترین کتاب‌های نوشته‌شده در موضوع تراریخته‌ها با بیش از 1150 منبع علمی و در واقع یکی از بهترین کتاب‌های پنجاه سال اخیر جهان است که مورد تحسین دانشمندان قرار گرفته است. در این کتاب به چگونگی ایجاد بذرهای تراریخته و کنترل...

Immunostimulatory CpG Oligonucleotides as an Adjuvant with Recombinant DNA Vaccine Encoding Hepatitis C Virus Core Protein Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran The immunogenicity and protective efficacy of DNA vaccines have been amply demonstrated in numerous animal models against infectious d...

The immunogenicity and protective efficacy of DNA vaccines have been demonstrated in numerous animal models of infectious diseases. In order to increase the potency of DNA vaccines, in this study, conventional adjuvants such as aluminium phosphates, dendro-some, CpG motif and mixture of aluminium phosphate and CpG motif have been tested. Female BAL...

Young Scientist Awards for Bacteriology Conference Ali Karami Journal of Applied Microbiology and Biochemistry 2019 Vol.3 No.2 Professor, Department of Medical Biotechnology and Genetic Engineering, Baqiyatallah University of Medical Sciences, Iran, E-mail:

Nearly 1.35 million people die each year due to traffic crashes, 50% of which belong to vulnerable users (pedestrians, cyclists and motorcyclists). Considering the importance of pedestrian safety at intersections that are an important part of the urban pedestrian network, it is necessary to take steps to increase pedestrian safety. The purpose of t...

پروتوکل عملیاتی کنترل موج سوم کرونا راهنمای علمی و عملیاتی برای مدیران و مردم دکتر علی کرمی استاد دانشگاه علوم پزشکی بقیه ا... (عج) متخصص بیوتکنولوژی پزشکی، مهندسی ژنتیک و تهدیدات زیستی و دفاع بیولوژیک 17 مهر ماه 1399

Effective Management of COVID-19 in Iran Ali Karami. Abstract : On 30 December 2019, a cluster of viral pneumonia cases of unknown origin emerged in Wuhan, Hubei Province of China which soon spread across the world.1 From the first COVID-19 case in Iran, the Iranian President ordered health minister to set up a national committee for managing coron...

On 30 December 2019, a cluster of viral pneumonia cases of unknown origin emerged in Wuhan, Hubei Province of China which soon spread across the world.1 From the first COVID-19 case in Iran, the Iranian President ordered health minister to set up a national committee for managing coronavirus epidemic. In his decree, President urged the Iranian Heal...

On 30 December 2019, a cluster of viral pneumonia cases of unknown origin emerged in Wuhan, Hubei Province of China which soon spread across the world. (1) From the first COVID-19 case in Iran, the Iranian President ordered health minister to set up a national committee for managing coronavirus epidemic. In his decree, President urged the Iranian H...

A Summary of Effective Management of COVID-19 in Iran Collaboration of Civil and Defense forces. Ali Karami January-June 2020 Volume: 13 Issue: 2 Invited Articles Ali Karami: is a professor of Molecular Biology (Medical Biotechnology), Genetic Engineering and Bioinformatic Research Center of Molecular Biology, Baqyiatallah University of Medical Sc...

به ریشه یابی واژه توهم توطئه پرداختیم که نتیجه آن بسیار جالب بود. پروفسور مارک کریسپین میلر استاد مطالعات رسانه ای دانشگاه نیویورک در ا 2004 که به دلیل ادعای به سرقت بردن آرا جان کری، برای افزایش آرای جرج دبلیو بوش و رسیدن به قدرت به توهم توطئه متهم شد در این مورد بسیار بررسی کرده است او در این مورد کتاب هایی با عنوان بازنده همه را می گیرد "Loser T...

این مطلب مهم را همه فرماندهان و نیروهای جان بر کف انتظامی، نظامی، دفاعی، پاسداران، نیروهای امنیتی، اطلاعاتی، سربازان گمنام امام زمان و نیروهای خدماتی، امدادی، اورژانس، هلال احمر و آتش نشانی و بسیجیان و نیروهای داوطلب حتما حتما مطالعه کنند. راه پیمایی عظیم اربعین حسینی و حرکت میلیون ها نفر بسوی زیارت بارگاه مقدس ابا عبدالله ع و ابولفظل العباس در عر...

One of the most important needs of pedestrians is safety at crossing points, especially at intersections. Intersections are important parts of the urban road network because any disruption in them reduces the capacity of the entire network. The main objective of this research is to propose an appropriate method for prioritizing urban intersections...

An outbreak of tularemia or plague like syndrome occurred in the province of Sistan and Balochistan of Iran, from May to June 2007. Tularemia and plague had not been reported in this region for last 100 years and before. Thirteen eight cases were identified with ulcer glandular syndrome dominant to all cases with age from children to elderly ages....

Osteoarthritis (OA) is a common chronic disorder among elderly people that affects joints such as the knee and hip in particular. The objective of the current study was to examine the efficacy of an intervention based on a theory of planned behavior (TPB) in improving health-related quality of life in middle-age and older adults with this condition...

همه چیز در باره سبک زندگی سالم و تغذیه سالم سبک زندگی چیست اصول سبک زندگی سالم پیشگیری مقدم بر درمان کارهایی که باید برای سبک زندگی سالم انجام دهید Healthy Life Style

محصولات دستکاری شده ژنتیک یا تراریخته، چندین سال است وارد سفره غذایی مردم ایران شده است بدون آن که مردم و مصرف کنندگان از ان مطلع باشند. علیرغم اینکه عده‌ای موافق تولید این گونه محصولات هستند، امااستفاده از گیاهان تراریخته منتقدینی نیز دارد.از آن جایی که ساختار ژنتیکی گیاهان تراریخته از طریق مهندسی ژنتیک تغییر می‌یابد و این تغییر معمولا در جهت بهبو...

Introduction: Lyme and MS are diseases of central nervous system. Many genetic and environmental factors such as viruses, bacteria and genes of MHC or HLA systems have been suggested for MS. The cause of Lyme is a spiroctall bacterium called Borreli bugdorferi which causes similar symptoms to MS in acute stages. So, this bacterium can have an impor...

Detection and identification of microbial agents is important step for accurate diagnosis of infectious diseases for treatment or prevention from spread to the society. Classical methods like culture, subculture, biochemical, immunological or other typing process are very time consuming, labor intensive, expensive and results are presented very lat...

Abrin, known as a ribosome inactivating protein (RIP), is a high cytotoxic plant protein. The high lethality, low cost, and easy access to this plant and its seeds have led to this toxin to be used in crimes and terrorist acts. Since, obtaining purified toxins requires advanced laboratory equipment and complex procedures, it seems that the perpetra...

This Book Contains several chapter on Bioterrirism , Introduction, History, Novle Boi Threats , Passive defense in Biological Threats

Abstract Background and Objective: Biological weapons, like other weapons of mass destruction such as chemical, nuclear and radiological are very dangerous. In recent years, they are employed in biotterrorist attacks by many countries because of some properties such as: the ability to make massive injury, having latent period, creating a prolonged...

Enhancing public health is the major goal of all countries for public welfare. Passive defense is peaceful strategy to tackle emerging and reemerging threats against public safety and security. In this study we have conducted research plan to compare public health strategy of various nations from developed to under development countries on the base...

Detection and identification of microbial agents is important step for accurate diagnosis of infectious diseases for treatment or prevention from spread to the society. Classical methods like culture, subculture, biochemical, immunological or other typing process are very time consuming, labor intensive, expensive and results are presented very lat...

The BICC’s Global Militarization Index (GMI) 2012 states that the Middle East is the region with the highest level of militarization in the world. Five of the top ten countries in the GMI are situated in the Middle East: Israel (position 1), Syria (position 3), Jordan (position 5), Kuwait (position 8), and Saudi Arabia (position 10). Followed by ot...

An outbreak of tularemia or plague like syndrome occurred in the province of sistan and baluchestan of, Iran, from May to June 2007. Tularemia and plague had not been reported in this region for last 100 years and before. Thirteen eight cases were identified with ulcero glandular syndrome dominant to all cases with age from children to elderly ag...

Increasing threat of emerging infectious disease like, Ebola, Marburg, different strain of Influenza and other viral and bacterial agents with no active prevention available needs critical consideration to develop a new type of vaccine to protect people for a long time. Classic vaccine technology with very slow progress reveals its weakness to deve...

Lyme disease is a bacterial infection caused by the spiral-shaped bacterium Borrelia burgdorferi. We investigated the presence and prevalence of Borrelia species in ticks from the southern England. One hundred fifty-five cases (103 adult and 52 nymphal ticks) were collected from animal carcases. The midguts were removed, cultured in Barbour/Stoenne...

تهدیدات سلاح های کشتار جمعی و به خصوص عوامل شیمیایی، بیولوژیک و هسته ای به عنوان تهدیدی در عرصه جنگ نامتقارن و ناهمطراز تهدیدی جدی و قابل تامل است. متأسفانه با وجود معاهدات بین المللی منع توسعه و استفاده از این سلاح ها نه تنها شاهد توسعه تحقیقات در این زمینه هستیم بلکه انواع جدیدی در جنگ ها به کار گرفته می شوند که جدید ترین آنها در عراق، افغانستان...

Biohazards are divided to Natural man made,Before and During these incidents we need to organize our healthcare system to prevent or be prepared to act as soon as possible. on Natural biological incidents like epidemics or pandemics we need network of health system for Biosurveillance and ..... in this course we educate managers and decision makers...

Salmonellas are a group of Entrobacteriace that cause Infection disease in human and animals .Thyphoid, Bacteriemi ,Entrocolit and Salmonelose are cause by this bacteria turned to be a major health problem in developing countries ,there for quick , accurate ,reliable and on time diagnosis seem to be vital to prevent it from spreading widely and bec...

One of the major goals of the bio defense programs of every nation is to prevent from threats of Biological agents. The main questions are: which agent? by whom? where? and how it will be delivered? it is very difficult to answer these questions. Various numbers of biological agents, simplicity of agent development, mass production ad delivery and...

Detection and identification of microbial agents is important step for accurate diagnosis of infectious diseases for treatment or prevention from spread to the society. Classical methods like culture, subculture, biochemical, immunological or other typing process are very time consuming, labor intensive, expensive and results are presented very lat...

Abstract Spirochaetaceae is a family of spirochetes that cause syphilis, Lyme disease, epidemic and endemic relapsing fever, leptospirosis, swine dysentery, and periodontal disease. The spirochetes are presently classified as members of class Spirochaetes in the order Spirochaetales and are divided into three major phylogenetic groupings or familie...

Rapid Detection and Identification of Microbial Agents Professor Ali Karami Research Centre of Molecular Biology, Baqiyatallah University of Medical Sciences. Karami@bmsu.ac.ir Detection and identification of microbial agents is important step for accurate diagnosis of infectious diseases for treatment or prevention from spread to the society. Cl...

Rapid Detection and Identification of Microbial Agents Professor Ali Karami Research Centre of Molecular Biology, Baqiyatallah University of Medical Sciences. Detection and identification of microbial agents is important step for accurate diagnosis of infectious diseases for treatment or prevention from spread to the society. Classical methods...

در چند دهه گذشته بررسی جنبه های ژنتیکی رفتار به شدت مورد توجه دانشمندان قرار گرفته است و علوم دقیقی در ارتباط با کشف نقش وراثت و زنتیک افراد در رفتار و شخصیت آنان ایجاد شده است مانند: زنتیک رفتار، زنتیک سیستم عصبی و زنتیک روانپزشکی، اخیراً مطالبی در مورد نقش ژنتیک در گرایشات مذهبی مطرح شده است که در جوامع علمی بازخوردهای متفاوتی داشته است. حتی مقال...

Point Of Care (POC) Diagnostics from Bench to Market Ali Karami Research Centre of Molecular Biology, Baqiyatallah University of Medical Sciences. Karami@bmsu.ac.ir Point-of-care test (POC) also known as bedside, near-patient testing is defined as medical testing at or near the site of patient care.The current diagnostic laboratory system has been...

پیشرفت های چند دهه اخیر در حوزه زیست شناسی بخصوص زیست شناسی مولکولی، شناخت اسرار فعالیت های درون سلولی و فرایند های مولکولی پدیده های زیستی و بخصوص کسب توانمندی نوترکیبی ژن ها با فناوری مهندسی ژنتیک، زیست شناسی را از مرحله تحقیقات به حوزه کاربرد های مفید آن برای بشر و تولید محصولات مختلف در عرصه های بهداشت و درمان، کشاورزی و دامپروری، صنعت و محیط ز...

Using molecular adjuvants offers an attractive strategy to augment DNA vaccine-mediated immune responses. Several studies have revealed that an efficient HCV vaccine model should be able to induce both humoral and cell mediated immune responses targeting the conserved regions of the virus to circumvent the immune escape mutants. The beta chemokine...

Microbial typing is often employed to determine the source and routes of infections, confirm or rule out outbreaks, trace cross-transmission of healthcare-associated pathogens, recognize virulent strains and evaluate the effectiveness of control measures. Conventional microbial typing methods have occasionally been useful in describing the epidemio...

DNA microarrays consist of collection of DNA microscopic spots that In order to form an array attached to a solid surface such as glass, plastic or silicon chip. The pieces of fixed DNA considered as a searcher. In this technology it is possible to test sample against thousands probes for specific genes. With this ability, arrays accelerate the bio...

Salmonella typhi, Bacillus anthracis, and Yersinia pestis are some serious human pathogens, which their early diagnosis is of great importance. Salmonella typhi, Bacillus anthracis, and Yersinia pestis cause typhoid fever, anthrax, and plague respectively. These bacteria can be used to make biologic weapons. In this study, we designed a new and rap...

Aims: To provide a new reasonable measure for distinguishing between coding and non-coding regions of DNA sequences based on its fractal nature and self-similarity. Study design: After conducting background studies on the fractal structure of DNA sequences, the application of Detrended Fluctuation Analysis for identifying coding and non-coding regi...

Background: Cystatines have regulative and preventive roles on Cysteine Proteases which existing in all biologic fluids of the human body. The protein-coding genes in humans and mice are located on chromosomes 20. The purpose of the present study is cloning and sequencing of hman Cystatin Gene of human normal tissues isolated from Iranian samples f...

Background and Objectives: Salmonella typhi, Bacillus anthracis, and Yersinia pestis are potent human pathogenic bacteria that cause typhoid fever, anthrax, and plague,respectively. The classic microbiological methods for detection and identification of these agents are laborious and time consuming.Therefore, developing an accurate method for rapid...

To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously. Three important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method. According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in...

Abstract Aim: The aim of this study was to standardize the molecular detection Staphylococcus aureus enterotoxin C gene method. Materials and Methods: Based on gene bank data, the PCR molecular method was set up for detection of entC gene in Staphylococcus aureus isolated from human subjects (300 isolated strains were assayed). The PCR Product was...

largest genomes belongs to a very small creature, Amoeba dubia. This protozoan genome has 670 billion units of DNA, or base pairs. The genome of a cousin, Amoeba proteus, has a mere 290 billion base pairs, making it 100 times larger than the human genome. Human genome is 3000.000.000 base pair, if we publish it as a book it needs 1000 books of 100...

Introduction:With increasing threats of weapons of mass destruction (WMD) in future war especially biological threats against our country necessity of using novel technologies in crisis management against new warfare’s is very important. Passive defense as a peaceful and preventive strategy without any warfare is able to not only monitor the prospe...

INTRODUCTION: Several factors have been suggested to account for differences in the virulence of Helicobacter pylori infections in various populations. Evidence suggests the existence of different strains of H. pylori with different degrees of virulence. The present study aimed to investigate the gastric histopathology in Iranian patients infected...

Biological capabilities ( more specifically non- conventional biological capabilities and bio-weapons) are among the important challenges facing the Middle East. The overlap between Biological Weapons and Chemical warfare, the absence of verification regime for nuclear weapons are among the factors producing such a challenge. The paper discuss bios...

Aims: The aim of this study was to standardize the molecular detection Staphylococcus aureus enterotoxin C gene method. Methods: Based on gene bank data, the PCR molecular method was set up for detection of entC gene in Staphylococcus aureus isolated from human subjects (300 isolated strains were assayed). The PCR product was sequenced and were com...

Several factors have been suggested to account for differences in the virulence of Helicobacter pylori infections in various populations. Evidence suggests the existence of different strains of H. pylori with different degrees of virulence. The present study aimed to investigate the gastric histopathology in Iranian patients infected with H. pylori...

Objectives: The aim of this study was to determine the type of Streptococcus pneumoniae which is more prevalent in different diseases. Method: 115 Clinical isolates of Streptococcus pneumoniae were re – identified and serotyped with Quellung test as a one of the serotyping methods based on SSI protocol - Statens Serum Institute. The data were inser...

Lyme disease, or Lyme borreliosis, is an emerging infectious disease caused by bacteria belonging to the genus borrelia. Borrelia burgdorferi, in the strict sense. This book deals mostly with the molecular biology of the Lyme disease agent orrelia burgdorferi. It has been written by experts in the relevant field and is tailored to the need of resea...

Biosecurity covers a broad spectrum of potential risks and threats ranging from criminal activities to bioterrorism and espionage. This handbook focuses on the laboratory related activities and basic components of applied biosecurity that are relevant to all laboratory employees. The role played by laboratory managers and principal investigators in...

Janbazan Medical and Engineering Research Center (JMERC) as the head of a research group for CW victims. JMERC is under the organisation for veterans and war victims. Concurrently, I am volunteering for the Society for Chemical Weapons Victims Support (SCWVS), which is a Tehran-based NGO. AK: Can you tell us about the history of chemical attacks ag...

Background and purpose: Salmonella is a group of Enterobacterias that cause infectious diseases in human and animals. Typhoid, bacteremia, enterocolitis and salmonellosis which are caused by this bacterium are considered major health concerns in developing countries, such as Iran. Quick, accurate, reliable and early diagnosis is vital to prevent Sa...

Background&Objectives: Salmonellas are a group of Entrobactria that cause infectious diseases in human and animals Typhoid, Bacteriemi, Entrocolit and Salmonelose are caused by this Bacterias turned to be a major health problem in developing countries, such as our country Iran. Therefore, quick, accurate, reliable and on time diagnosis seems to be...

Aims: The goal of this research is standardizing molecular detection of the entC gene of Staphylococcus aureus isolated from human infections and determining its sequence. Methods: At first, the primer was prepared using the standard gene in the gene bank. Then, the molecular PCR method was set up to identify the entC gene in the Staphylococcus aur...

Background and Objectives: Staphylococcal enterotoxin D as a supper antigen is produced by infected samples of human and animal sources. The aim of this study was to standardize the detection methods for the Staphylococcus strain producing enterotoxin D. Materials and Methods: A PCR method was set up for detection of enterotoxin D gene (ent D) in S...

Abstract: Objective: The role of staphylococcal enterotoxin B (SEB) in food poisoning is well known, however its role in other diseases remains to be explored. The aim of this study is the molecular screening and characterization of the SEB gene in clinically isolated strains. Materials and Methods: In this experimentally study, 300 Staphylococcus...

Objective: The role of staphylococcal enterotoxin B (SEB) in food poisoning is well known, however its role in other diseases remains to be explored. The aim of this study is the molecular screening and characterization of the SEB gene in clinically isolated strains. Materials and Methods: In this experimentally study, 300 Staphylococcus aureus (S....

Polymerase chain reaction is the most widely used method for DNA amplification for the detection and identification of infectious diseases, genetic disorders and for other research purposes. However, it requires a thermocycling machine to separate the two DNA strands and then amplify the required fragment. Novel developments in molecular biology of...

To determine the levels of vascular endothelial growth factor isoform consisting of 165 amino acids (VEGF165) in Bronchoalveolar Lavage Fluid from Mustard Exposed Patients. Bronchoscopy with Bronchoalveolar Lavage was performed on sulphur mustard exposed patients. A total of 39 patients with documented exposure to Sulfur Mustard during the Iran-Ira...

Introduction and objective: Salmonellosis is responsible for large numbers of infections in both humans and animals. Conventional methods of isolation of Salmonella strains take 4-7 days to complete and are therefore laborious and require substantial manpower. Our main objective was to develop a rapid detection method using shortened PCR cycles in...

Introduction: Hepatitis C virus (HCV) is the major cause of acute and chronic hepatitis, cirrhosis, and hepatocellular carcinoma worldwide. Because of increasing number of infected patients throughout the world, various studies are concentrated on development of high quality diagnostic tests and genotyping in various regions of the world. Methods:...

INTRODUCTION: Antimicrobial drug resistance is increasing among Salmonella spp. and causes significant therapeutic problems in the treatment of diseases caused by this organism. The aim of this study was to determine the aminoglycoside resistance pattern of Salmonella spp. isolated from clinical cases in Tehran. MATERIAL-METHODS: Salmonella spp. st...

The use of cytokines as adjuvants has been shown to be a promising approach for enhancing DNA vaccine induced-immune responses. In this report, we investigate the administration of cytokines to modulate both humoral and cell-mediated immune responses elicited by an HCV-core plasmid DNA vaccine in Balb/c mice. Our studies indicate that the HCV-core...

Bioterrorism is defined as the intentional use of infectious disease agent and natural or synthetic biological toxin to harm individual people or to elicit widspread fear or intimidation of society

Rapid detection of natural and synthetic toxins is very critical for early warning and prevention from harmful side effects

Due major concern in dual use research and development in bio-toxins needs critical attention in legal, ethical, safety and bio security aspects