Ali Javadmanesh

Ferdowsi University Of Mashhad | FUM · Department of Animal Sciences

Background Bovine Seminal Ribonuclease (BS-RNase) is a unique member of the RNase A family found in the bovine seminal fluid. It is recognized for its antiviral and antitumor properties, which make it a potential therapeutic agent. Objective This study aimed to compare the stability of monomeric and dimeric forms of BS-RNase using in silico method...

Keywords Broiler Chicken Economic Traits Quantitative Trait Loci Protein-Protein Interaction The availability of genomic data, such as quantitative trait loci (QTL), has played a pivotal role in understanding the genetic components of various traits. This study aims to investigate critical and hub genes related to economic traits such as growth rat...

Introduction Identifying genomic regions under selection is the most challenging issue for improving important traits in animals. Few studies have focused on identifying genomic regions under selection in sheep. The aim of this study was to identify selective sweeps and to explore the relationship between these and quantitative trait loci (QTL) in...

Zinc (Zn) is a significant element of the reproductive system and is associated with several enzymes that regulate different metabolic pathways. Organic Zn can significantly affect polycystic ovarian syndrome (PCOS) pathogenesis. Insulin resistance (IR) is a common complication of PCOS. Mammalian target of rapamycin (mTOR), which controls crucial c...

In this study, we aimed to experimentally induce fatty liver disease in rainbow trout, Oncorhynchus mykiss, and then assessed the illness recovery process, growth, and changes in the expression of FAAH and ACADL genes in both healthy (0 [C2] and 4% apple cider vinegar [T4]) and diseased fish (0 [C1], 1 [T1], 2 [T2], and 4% [T3]) apple cider vinegar...

The present study aimed to evaluate the effect of thymol on growth performance and apparent total tract digestibility of nutrients in severely feed-restricted lambs. Twenty-one male Baluchi lambs were randomly blocked by live weight into three groups: control without feed restriction (CON), feed restricted (FR), and feed restricted plus thymol (FR...

Nivalenol (NIV) is a prevalent mycotoxin that poses challenges for effective mitigation using conventional approaches, including the use of mineral and organic adsorbents. Hence, it is recommended to explore enzyme-based approaches for the detoxification of these specific mycotoxins, necessitating prior bioinformatics investigations to inform subse...

Kirsten rat sarcoma viral oncogene homolog (KRAS) exhibits the highest mutation rate in colorectal cancer (CRC) and pancreatic cancer (PC), highlighting the need for a comprehensive understanding of KRAS-dependent pathogenesis. Given the regulatory role of long noncoding RNAs (lncRNAs) in gene expression, this study focused on constructing a compet...

Despite the identification of candidate genes influencing milk protein, the connections between genes and regulatory pathways remains elusive. This study aimed integrate findings from genome-wide association studies (GWAS) and RNA sequencing (RNA-Seq) through meta-analysis to pinpoint single nucleotide polymorphisms (SNPs) and genes responsible for...

Introduction Sex differences in prenatal growth may contribute to sex-dependent programming effects on postnatal phenotype. Methods We integrated for the first time phenotypic, histomorphological, clinico-chemical, endocrine and gene expression analyses in a single species, the bovine conceptus at mid-gestation. Results We demonstrate that by mid...

The present study aimed to evaluate the effect of thymol on growth performance and apparent total tract digestibility of nutrients in severely feed-restricted lambs. Twenty-one male Baluchi lambs were randomly blocked by live weight into three groups: control without feed restriction (CON), feed restricted (FR), and feed restricted plus thymol (FR...

Myostatin is a known negative regulator of muscle tissue growth. Thus, an inhibitor of myostatin may be therapeutically useful as an anabolic agent for the muscle tissue. A promising gene-silencing approach for gene therapy is DNA interference (DNAi), a sequence that is complementary to the promoter region of a target gene. To confer resistance to...

Current study hypothesized that dietary l ‐carnitine (LC) inclusion during the mating period ameliorates both metabolic status and reproductive performance of ewes. Seventy Baluchi ewes (52 ± 4.2 kg of bodyweight and 18 ± 6 months old of age) were enrolled in this study. Animals were randomly allocated into two dietary treatments, control (only bas...

Identification of selection signatures may provide a better understanding of domestication process and candidate genes contributing to this process. In this study, two populations of domestic and wild goats from Iran were analyzed to identify selection signatures. RSB, iHS, and XP-EHH statistics were used in order to identify robust selection signa...

Background Lactoferrin is a versatile protein with important modulatory functions in inflammation and immune response. This glycoprotein can bind and sequester iron and LPS, thereby intervening in certain signaling pathways and biological processes. In the present meta‐analysis, we aimed to pool experimental data regarding the immunomodulatory effe...

Mastitis is one of the costliest diseases in dairy farms caused by infection of different microorganisms such as Escherichia coli, Streptococcus uberis and Staphylococcus aureus. Promoters are significantly involved in regulating gene expression and shedding light on the mechanisms of transcriptional regulation in physiological and immunological pr...

The productivity of beef cows depends on early reproduction traits such as puberty and has an economic impact on the efficiency of production system. Imprinted genes modulate many important endocrine processes such as growth, the onset of puberty and maternal reproductive and behavior. The role of imprinted genes in puberty is a challenging subject...

Myostatin (MSTN) belongs to transforming growth factor-beta (TGF-) family as an autocrine/paracrine hormone produced mainly by muscle cells that inhibits muscle mass development. Follistatin is known to antagonize the function of TGF- ligands, such as MSTN and activin A. Therefore, the aim of this study was to predict the 3D structure of rat (Rat...

Awassi breed is an important sheep in the Middle East, but its lower twinning potential is a limitation against improvement. Due to the dynamic role of oocyte-expressed protein (OOEP) gene in controlling folliculogenesis, this study was conducted to evaluate the possible association between OOEP and twinning rate, as well as body measurement traits...

The lack of cost‑effective methods for producing antimicrobial peptides has made it impossible to use their high potential as a new and powerful class of antimicrobial agents. In recent years, extensive research has been conducted to decrease the cost of recombinant proteins production through microorganisms, transgenic animals, and plants. Well‑kn...

DNA-based approaches can now be utilized as low-risk methods to change gene expression. It appears that this approach has the ability to partially replace RNA-based approaches for altering gene expression, which in the majority of cases leads to immunological responses in patients. When utilized as a technique to silence target gene expression, DNA...

Exopolysaccharides are high molecular weight polymers composed of sugar subunits. Produced exopolysaccharides by lactic acid bacteria (LAB) play a significant role in improvement of organoleptic properties of fermented dairy products such as yogurt. Diversely, the probiotic function of these bacteria and the prebiotic properties of their produced b...

Habitat eradication and loss of animal species have created a new international hazard for wildlife conservation. National parks are considered as suitable places that can serve dual functions of biodiversity conservation and ecotourism. As recommended by the Food and Agriculture Organization (FAO) and ISAG, microsatellites have been used for anima...

In the present study, kashk samples were collected from two regions of Iran, Fars (Abadeh) and Razavi Khorasan (Kalat) provinces. Fifteen bacteria were isolated and physiological and bio-chemical assays were performed. After identification at the genus level, eight isolates were identified as Lactic Acid Bacteria (LAB) and subjected to molecular id...

Article type: Research Full Paper

The effects of curcumin and its nano-micelle form on body weight, insulin resistance, adiponectin, and blood biochemical parameters of streptozotocin-induced diabetic rats were studied. Fifty male wistar rats were induced diabetes and divided into 5 groups and treated with 1) no dietary supplements, 2 and 3) 40 and 80 mg curcumin/kg of feed, 4 and...

Keywords Semen quality Broiler breeder rooster Whey protein, Selenium Antioxidant enzymes activity The objective of this study was to investigate the effect of whey protein concentrate (WPC) and selenium (Se) supplementation on sperm quality, antioxidant enzymes activity and lipid peroxidation in seminal plasma, liver and testis of roosters. Forty-...

Introduction: Dairy milk is one of the most important economic products for any country. Also, milk fat has high impact on taste of milk and dairy products. The liver in ruminant animals including dairy cows plays an important role in carbohydrates metabolism, fats, vitamins, hormones, and etc. The absorbed nutrients pass through the liver from the...

Background: Abuse of growth hormone (GH) is expanding in exercising populations due to its lipolytic and anabolic actions. The purpose of this study was to examine the interactive effect of exercise training and GH administration on histopathological and functional assessment in the liver of male Wistar rats. Methods: Forty-eight male Wistar rat...

DNA methylation is a fundamental epigenetic process and have a critical role in many biological processes. The study of DNA methylation at a large scale of genomic levels is widely conducted by several techniques that are next-generation sequencing (NGS)-based methods. Methylome data revealed by DNA methylation next-generation sequencing (mNGS), sh...

The production of polysaccharides derived from lactic acid bacteria (LAB) can be a valuable alternative to current polysaccharides. In this study, eight samples of Kishk (traditional dairy product) were collected in sterile conditions and directly cultured on MRS agar medium. Following purification and examination of microscopic, macroscopic charac...

Objectives: Early, specific, and sensitive detection methods of COVID-19 are essential for force stopping its worldwide infection. Although CT images of the lung and/or viral RNA extraction followed by real-time reverse-transcriptase-polymerase chain reaction (rRT-PCR) are widely used; they have some limitations. Here, we developed a highly sensit...

KRAS mutation is responsible for 40–50% of colorectal cancers (CRCs). RNA-seq data and bioinformatics methods were used to analyze the transcriptional profiles of KRAS mutant (mtKRAS) in comparison with the wild-type (wtKRAS) cell lines, followed by in-silico and quantitative real-time PCR (qPCR) validations. Gene set enrichment analysis showed ove...

Importance of the work: Sheep are a major source of mutton production. Therefore, recognizing the genes that influence muscle growth rates in sheep is useful in breeding programs. Objectives: To identify the genes regulated by long non-coding RNAs (lncRNAs) that are effective in sheep skeletal muscle growth and development. Materials & Methods: In...

Abstract: Hilsa species are broadly described as largely anadromous fish with a large valuable population size. It is well-known as one of the most critical commercial and occurs in marine, estuarine and riverine environments Hilsa shad, Tenualosa ilisha locally known as Sbour, migrates to the freshwater environment of the Shatt Al-Arab River syste...

Long non-coding RNAs (lncRNAs) compose a plentiful category of transcripts that have gained increasing importance because of their roles in different biological processes. Although the function of most lncRNAs remains unclear. They are implicated in epigenetic regulation of gene expression, including muscle development and differentiation. We aimed...

Hilsa species are broadly described as largely anadromous fish with a large valuable population size. It is well-known as one of the most critical commercial and occurs in marine, estuarine and riverine environments Hilsa shad, Tenualosa ilisha locally known as Sbour, migrates to the freshwater environment of the Shatt Al-Arab River systems for bre...

Antibiotic resistance is a looming public health crisis especially in animal industry. Hence, researchers are looking for natural alternatives such as Antimicrobial peptides (AMPs). Nowadays, AMPs, also known as host defense peptides, are short and generally positively charged peptides found in a wide variety of life forms from microorganisms to hu...

Naturally, Fertility characteristics such as ovulation rate, litter size, total number of lambs born, stillbirth and age at first puberty are regulated by different genes with different effects in sheep and all have a significant impact on the economy of the sheep industry [1]. The development of DNA-based marker technology and genomic tools opened...

Myostatin is a transforming growth factor-beta (TGF-beta) family member that plays roles as a negative regulator of muscle mass development. Follistatin is a myostatin-binding protein that can inhibit myostatin activity to promote muscle growth [1]. This is an important way for increasing growth especially in demo horses. Here, we aimed to predicti...

Introduction: Poultry and meat products are the largest sources of non-typhoid salmonella infections in most countries. Studies have shown that raw foods of animal origin, especially poultry and its products, are the main source of contamination of kitchens and restaurants. In terms of growth conditions, these microorganisms are resilient bacteria...

Article Info Importance of the work: While several studies investigated the transcriptome of bovine mammary tissue; the cellular, biological processes and metabolic pathways of differentially expressed genes involved in lactation period in bovine have not been completely considered. Objectives: The objective was to identify the differentially expre...

In present study, genetic diversity of domestic and wild Iranian sheep and goats were evaluated using inbreeding coefficients (FGRM, FHOM and FROH), linkage disequilibrium (LD), and effective population size (Ne). Genomic data of Iranian sheep and goat species were obtained from the NEXTGEN project. Animals consisted of 20 domestic sheep (Ovis arie...

This study aimed to monitor the effect of including rumen-protected L-carnitine (Carneon 20 Rumin-Pro, Kaesler Nutrition GmbH, Cuxhaven, Germany) in the transition diet on the productive and metabolic responses of multiparous high-producing Holstein dairy cows. Thirty-two multiparous cows were allocated in a completely randomized design to receive...

The effect of different levels of Scrophularia striata extract in normal and challenged conditions with E. coli on performance, carcass characteristics, cellular immune response, blood antioxidant status, intestinal histo-morphometry, and microbial population (E.coli and Lactobacillus) of Cobb 500 broilers were evaluated. The experiment was perform...

Abstract Background and objectives: Mastitis is an inflammatory disease in dairy cows that occurs in response to infectious factors. This inflammatory disease has a high negative economic impact on the dairy industry. In recent decades, somatic cell score has been used as an indirect method to control mastitis. Resistance to infection disease may b...

In this study, the antimicrobial effect of exopolysaccharide (EPS) extracted from Enterococcus strains [E. durans K48 (MT437,248), E. faecium R114 (MT437,249) and E. faecium T52 (MT437,250)] isolated from Kishk was applied against some foodborne pathogenic bacteria using well diffusion and microdilution methods. The antioxidant activity of EPS was...

Clostridium perfringens-induced necrotic enteritis (NE) is an economically important disease of broiler chickens. The present study evaluated the effect of C. perfringens on the intestinal histomorphometry, enteric microbial colonization and host immune responses using three experimental NE reproduction methods. The experimental groups consisted of...

Article Info AGRICULTURE AND NATURAL RESOURCES The genetic similarity of anadromous Hilsa shad (Tenualosa ilisha) samples from four stations along the Shatt Al-Arab River was assessed using partial sequencing of the mtDNA cytochrome b gene to describe population structures and to compare samples. Two-way sequencing was conducted on 70 samples from...

Cationic antimicrobial peptides are being developed as a promising class of antimicrobial sub-stances. The introduction of a new antibiotic component requires a comprehensive study of its properties so that it can be relied upon to continue laboratory procedures and clinical trials on laboratory animals or human volunteers. Antimicrobial activity o...

Abstract Antagonistic interactions among different functional guilds of nematodes have been recognized for quite some time, but the underlying explanatory mechanisms are unclear. We investigated responses of tomato (Solanum lycopersicum) to two functional guilds of nematodes - plant parasite (Meloidogyne javanica) and entomopathogens (Heterorhabdit...

The use of anticancer peptides (ACPs) as an alternative/complementary strategy to conventional chemotherapy treatments has been shown to decrease drug resistance and/or severe side effects. However, the efficacy of the positively-charged ACP is inhibited by elevated levels of negatively-charged cell-surface components which trap the peptides and pr...

Identifying the genomic regions which are under selection for improving economically important traits in animals are the most important, and challenging issue in population genetic. However, few genomic regions have been identified nowadays, that positively selected in sheep. The aim of this study was to identify genomic regions under selection bet...

KRAS is one of the most widely prevalent proto-oncogenes in human cancers. The constitutively active KRAS oncoprotein contributes to both tumor onset and cancer development by promoting cell proliferation and anchorage-independent growth in a MAPK pathway-dependent manner. The expression of microRNAs (miRNAs) and the KRAS oncogene are known to be d...

We investigated responses of tomato to two functional guilds of nematodes - plant parasite (Meloidogyne javanica) and entomopathogens (Heterorhabditis bacteriophora, Steinernema feltiae belowground, and S. carpocapsae) - as well as a leaf mining insect (Tuta absoluta) aboveground. Our results indicate that entomopathogenic nematodes (EPNs): 1) indu...

Heat stress is one of the main environmental factors causing economic losses to the poultry industry, as it reduces growth and production performance of chickens. The heat shock proteins (HSPs) involved in the key cellular defense mechanisms during exposure in hot environmental conditions. Elevated level of expression of HSPs helps to overcome the...

A large number of genome-wide association studies (GWAS) in livestock, especially in dairy cows, provide favorable conditions to integrate multiple independent studies. Methods such as meta-analysis provide the identification of effective QTLs with higher precision and power. A meta-analysis for milk production traits between different countries wa...

There is a continuing need to prevent the increasing use of common antibiotic and find the replacement to combat the drug/antibiotic resistant bacteria such as antimicrobial peptides (AMPs) such as thanatin peptide. In this study, recombinant thanatin peptide was expressed in the HEK293 cell line. Then the antimicrobial properties of this peptide o...

In most bacteria, Glutamate decarboxylase (GAD) activity significantly reduces at pH > 6. Since L-glutamate is neutral to slightly alkaline, a large amount of hydrochloric acid must be added to the reaction mixture. Acidic reaction conditions greatly increase the corrosion of production equipment and shorten the life of manufacturing equipment. The...

One of the most promising ways to diagnose cancer especially colorectal cancer (CRC) is to trace its epigenetic events. In this article, a discovery step for detection of methylated DNA markers (MDMs) was performed using SureSelectXT Methyl-Seq in CRC case and control groups in addition to several methylation profiling datasets (GSE48684, GSE53051,...

Several studies performed on the transcriptome of bovine mammary tissue. However, biological, cellular processes and metabolic pathways of differentially expressed genes involved in milk production of bovine mammary glands are not completely discovered. Finding the essential genes and pathways may help to find new mechanisms as well as new markers...

Objective Salmonella is a Gram-negative, anaerobic, flagellated bacterium of Salmonella type in Enterobacteriaceae family, which is known as a zoonosis infectious agent. In recent years, different types of antibiotics have been used to overcome the Salmonella infection; however, the increasing number of antibiotic-resistant bacteria, the provision...

Nutritionists have liberty to choose various feeds for formulating a balanced ration depending upon the nutritive value, availability and feed cost. Although final target in an alternative ration is to obtain similar energy, protein and other nutrients, it would be favourable to consider virtual water (VW) requirement which must be spent while maki...

Article Info AGRICULTURE AND NATURAL RESOURCES Associations were identified between the genetic polymorphism of the High Mobility Group AT-hook 2 (HMGA2) gene and growth traits in Awassi and Karakul breeds. Four polymerase chain reaction amplicons were designed to cover the coding regions of this gene. Several growth traits were analyzed every 3 mt...

Curcumin can be used for diabetes treatment. However, it rapidly degrades and has low absorption in gastrointestinal system. Its nanomicelle form might improve its efficiency. Therefore, they both assessed in streptozotocin-induced diabetic rats. Fifty male wistar rats were induced diabetes and divided into 5 groups and treated with 1) no dietary s...

Gamma amino butyric acid (GABA) is used as drugs, food ingredients, and dietary supplements. l-glutamate is converted to GABA by the decarboxylation reaction, which is catalyzed by the glutamate decarboxylase (GAD). Escherichia coli is widely being used to express proteins. However, without appropriate signal peptide, it cannot be applied for secre...

Gamma amino butyric acid (GABA) is used as drugs, food ingredients, and dietary supplements. l-glutamate is converted to GABA by the decarboxylation reaction, which is catalyzed by the glutamate decarboxylase (GAD). Escherichia coli is widely being used to express proteins. However, without appropriate signal peptide, it cannot be applied for secre...

Computational prediction of signal peptides is one of the most important steps in genetic engineering experiments. The periplasmic expression cause the reducing in the inherent destructive behavior of Bofurin I against its host and also reducing its susceptibility to proteolytic degradation. In order to predict the best signal peptides for expressi...