Ali Assifaoui

Ali Assifaoui
University of Burgundy | UB · Unité Mixte de Recherche Procédés Alimentaires et Microbiologiques (PAM)

Professor

About

53
Publications
10,023
Reads
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1,049
Citations
Citations since 2017
21 Research Items
784 Citations
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
Additional affiliations
September 2007 - present
UMR PAM Procédés Alimentaires et Microbiologiques
UMR PAM Procédés Alimentaires et Microbiologiques
Position
  • Professor (Associate)
September 2002 - present
University of Burgundy
Position
  • Professor (Associate)

Publications

Publications (53)
Article
Full-text available
Linseed oil-based composite films were prepared with cinnamaldehyde (Cin) using a modified clay (organoclay) through in situ polymerization, which is the result of the interaction between Cin and organoclay. The incorporation of organoclay reduces the polymer chain's mobility and, therefore, increases the thermal stability of the composite films. I...
Article
We performed molecular dynamics (MD) simulations of octameric galacturonate, GalA8, chains in the presence of Ca2+ in a ratio of R = [Ca2+]/[GalA] = 0.25 in order to determine to which extent the popular "egg-box model" (EBM) is able to describe the association between Ca2+ cations and polygalacturonate (polyGalA) chains. To this aim, we slightly r...
Poster
Full-text available
Interactions between proteins, phytic acid and calcium in Fava Bean concentrate
Article
Diffusions in gels are of prime importance, but their measurements are mainly focused on the diffusion in the pores or through the mesh of the gels. In this study, we performed a deeper dynamic analysis of the water in close interaction with the fibers structuring two heterogeneous polygalacturonate hydrogels formed by Ca and Zn ions (crosslinking...
Article
In this study, the interaction of exopolysaccharides from Leuconostoc mesenteroides P35 (EPS-LM) with Escherichia coli heat-labile enterotoxin B-pentamer (LTB) was investigated at different concentrations and temperatures by using surface plasmon resonance (SPR) and molecular docking approaches. FT-IR spectral analysis together with HPTLC analysis...
Article
This work aims to synthesize polygalacturonate-based magnetic iron oxide nanoparticles (INP-polyGalA). The synthesis consists of the diffusion of both Fe²⁺ and Fe³⁺ at a molar ratio of 1:2 through polyGalA solution followed by the addition of an alkaline solution. To form individual nanoparticle materials, the polyGalA concentration needs to be bel...
Article
Nowadays, legumes are considered as a good source of plant-based proteins to replace animal ones. They are more favorable regarding environmental aspects and health benefits, therefore many people consider moving toward a greener diet. Interestingly, recent consumer trends are promoting pea and faba bean as alternatives to soybean. Both are rich in...
Article
Full-text available
Alginate is a polysaccharide obtained from brown seaweed that is widely used in food, pharmaceutical, and biotechnological applications due to its versatility as a viscosifier and gelling agent. Here, we investigated the influence of the addition of glucose on the structure and mechanical properties of alginate solutions and calcium-alginate hydrog...
Article
Full-text available
This study aims to encapsulate the fungicide carbendazim using a biodegradable polymer (pectin). First, we have obtained calcium pectinate beads (CPG-Carb) by ionotropic gelation using calcium ions as a crosslinking agent. These beads were then coated with silica starting from tetraethoxysilane (TEOS), by a sol-gel process to form hybrid beads (CPG...
Chapter
Pectin as a natural biopolymer is extensively used for pharmaceutical and biomedical applications, essentially due to its gelling properties that could be influenced by pectin sources and extraction methods. This chapter focuses on an overview of pectin drug delivery systems classified by their administration routes. Oral drug delivery systems have...
Article
Full-text available
In this study, we show that calcium pectinate beads (CPB) allow the formation of 20 µm spherical microcolonies of the probiotic bacteria Lacticaseibacillus paracasei (formerly designated as Lactobacillus paracasei ) ATCC334 with a high cell density, reaching more than 10 log (CFU/g). The bacteria within these microcolonies are well structured and a...
Article
Full-text available
Bacterial strains of the Lactobacillaceae family are widely used as probiotics for their multifaceted potential beneficial properties. However, no official recommendations for their clinical use exist since, in many cases, oral administrations of these bacteria displayed limited beneficial effects in human. Additional research is thus needed to imp...
Article
This work reports on an in-depth characterization of the nano- and microstructure of extruded starch foams loaded with the microalga Spirulina (1, 5 and 10 wt%), as well as the implications of Spirulina incorporation on the textural properties of the foams. Due to the gelatinization process occurring during extrusion, the crystalline and lamellar s...
Article
We show here how the structure of polygalacturonate (polyGalA) hydrogels cross-linked by Ca2+ cations via external gelation controls the loading and the release rate of betalactoglobulin (BLG), a globular protein. Hydrogels prepared from a polyGalA/BLG solution are found similar to those obtained from a polyGalA solution in our previous study (Mair...
Article
The local structure of Fe²⁺ in Fe²⁺-polygalacturonic acid (polyGalA) hydrogels has been studied by coupling Extended X-ray Absorption Fine Structure (EXAFS) spectroscopy and molecular dynamics (MD) simulation. The EXAFS fitting results reveal an octahedral coordination geometry of Fe²⁺ both in aqueous solution and in the hydrogel, with similar Fe-O...
Article
We show here how the nature of various divalent cations M2+ (Ca2+, Zn2+ or Fe2+) influences the structure and mechanical properties of ionotropic polygalacturonate (polyGal) hydrogels designed by the diffusion of cations along one direction (external gelation). All hydrogels exhibit strong gradients of polyGal and cation concentrations, which are s...
Poster
Extrusion is used in many different industrial sectors such as packaging, foods, coatings, plastics, paper and pulp and ceramics. It is a process where the materials under different conditions are forced to flow through a shaped die to produce various products. The understanding of the mechanisms and physical transformations taking place during the...
Article
We designed stable and highly reproducible hydrogels by external unidirectional diffusion of Fe²⁺ ions into aqueous solutions of polygalacturonate (polyGal) chains. The Fe²⁺ ions act as cross-linkers between the Gal units in such a way that both the molar ratio R ([Fe²⁺]/[Gal units] = 0.25) and the mesh size of the polyGal network at the local scal...
Article
Polysaccharide-based hydrogels were prepared by the diffusion of divalent cations (X²⁺) into the polygalacturonate (polyGal) solution through a dialysis membrane. The diffusion of four divalent cations (Mg²⁺, Ca²⁺, Zn²⁺ and Ba²⁺) was investigated. The polyGal gel growth was studied as a function of the initial cation concentration by both viscoelas...
Article
Carrageenans are a family of sulphated cell wall polysaccharides extracted from seaweeds and are widely used in different industrial sectors. Relative to κ-carrageenan (κ-car) and ι-carrageenan (ι-car), the ionic binding behavior of λ-carrageenan (λ-car) is far less studied. In this work, the interaction and binding behavior between λ-car and metal...
Article
Three polyuronates: amidated low methoxyl pectin (ALMP), low methoxyl pectin (LMP) and polygalacturonic acid (PGA) were used to encapsulate rutin in pellets, and they were characterized by different techniques (macroscopic properties, calcium and rutin release). The ability of the three polyuronates to bind calcium ions and the viscoelastic propert...
Poster
Study of the Formation of Polygalacturonate Gels in the Presence of Ferrous Iron by Small Angle Neutron Scattering (SANS)
Article
In this work, we have investigated the interactions between polygalacturonate (polyGal) and four divalent cations (M(2+)= Ba(2+), Ca(2+), Mg(2+), Zn(2+)) that differ in size and affinity for water. Our results evidence that M(2+)-polyGal interactions are intimately linked to the affinity of M(2+) for water. Mg(2+) interacts so strongly with water t...
Article
Chitosan and fish gelatin were used to formulate active biobased films containing an antioxidant (coumarin). After drying, the films were irradiated at 40 and 60 kGy using an electron beam accelerator. The effect of irradiation on the film properties as well as the coumarin release mechanism were investigated and compared with the control. Electron...
Article
This work deals with the study of the release kinetics of antioxidants (ferulic acid and tyrosol) incorporated into chitosan-gelatin edible films after irradiation processes. The aim was to determine the influence of electron beam irradiation (at 60 kGy) on the retention of antioxidants in the film, their release in water (pH=7) at 25 °C, in relati...
Article
Full-text available
In this paper, we compare the interactions between low methoxy pectin (LMP) and either Ca2+ or Zn2+ in semi-dilute solutions. Intrinsic viscosity and turbidity measurements reveal that pectin-calcium solutions are more viscous, but yet less turbid, than pectin-zinc ones. To get a molecular understanding on the origin of this rather unexpected behav...
Article
Full-text available
In this work, the adsorption of a small globular protein (betalactoglobulin, BLG), on a natural montmorillonite clay (Mt) was investigated in acidic buffer (pH=3). The combination of different characterization techniques such as zetametry, X-ray diffraction, transmission electronic microscopy, fluorescence and solid state nuclear magnetic resonance...
Data
Full-text available
The aim of this work is to develop novel organic–inorganic hybrid beads for colonic drug delivery. For this purpose, calcium pectinate beads with theophylline are prepared by a cross-linking reaction between amidated low-methoxyl pectin and calcium ions. The beads are then covered with silica, starting from tetraethyoxysilane (TEOS), by a sol–gel p...
Data
This report focuses on cold-set gelation of pea (Pisum sativum L.) proteins and related globulin fractions, namely Vicilin 7S and Legumin 11S. Protein thermal denaturation and aggregation were investigated using differential scanning calorimetry (DSC) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis, and sulfhydryl (S-)...
Data
The physicochemical properties of a native, globular plant protein–linear anionic polysaccharide aqueous system at 20 °C were investigated in conditions where biopolymers carry a net negative charge (pH 7.2, 0.1 M NaCl). The pea proteins–sodium alginate mixtures showed a phase separation mainly by thermodynamic incompatibility, characterized at bot...
Article
The heat-induced denaturation and aggregation of mixed pea globulins (8 %, w/w) was investigated using differential scanning calorimetry (DSC), SDS-PAGE, and size-exclusion chromatography (SEC-HPLC). DSC data showed that the pea proteins denaturation temperature (Td) was heating-rate dependent. The Td value decreased of about 4°C by lowering the he...
Article
The aim of this work is to develop novel organic-inorganic hybrid beads for colonic drug delivery. For this purpose, calcium pectinate beads with theophylline are prepared by a cross-linking reaction between amidated low-methoxyl pectin and calcium ions. Then beads are covered with silica starting from tetraethyoxysilane (TEOS) by a sol-gel process...
Article
Full-text available
Soluble and natural mixed pea proteins (PP) were extracted from defatted pea seeds according to acidic precipitation (PPP) or ultrafiltration/diafiltration (PPDF) procedures. The isolates contained proteins with a low level of denaturation. Mixed pea globulins isolates presented quite similar solubility and thermal profiles, also a similar polypept...
Article
Zinc-pectinate beads are interesting drug carriers for oral delivery. In order to investigate their in vitro and in vivo release behaviour, ionotropic gelation was used to entrap theophylline into calcium- or zinc-pectinate beads. Beads were investigated in vitro for their particle properties, especially the release kinetic in different media, and...
Article
The aim of this study was to investigate drug release from calcium and zinc pectinate beads and to understand the impact of medium electrolytes during drug transfer. A potential drug carrier for colonic drug delivery (rutin) was prepared with calcium and zinc pectinate beads and was tested in three different simulated intestinal fluids (pH 7.3) wit...
Article
Calcium and zinc pectinate gels were prepared using a method which allowed calcium or zinc to diffuse from the cross-linking solution through a dialysis membrane to form a gel with amidated low-methoxyl pectin. The gel thus obtained was then dried, and the film structure was studied using FTIR spectroscopy as a function of the cation content (0%, 5...
Article
In this article, we were interested in the study of biscuit dough after kneading. Previous studies have shown that the dynamic properties of water and its distribution within dough influence the rheological behaviour and the machinability of dough. The viscoelastic characteristics of dough were determined with dynamic mechanical thermal analysis, w...
Article
Biscuit dough is a complex system containing numerous components in different states, such as starch, gluten, lipids (flour constituents), sugars, fats and water. Proton mobility in these dough systems was studied using low field 1H nuclear magnetic resonance (NMR). Transverse relaxation times (T2) were obtained from single pulse (FID) and Carr-Pur...
Article
Amorphous ethyl cellulose exhibits three secondary relaxations at temperatures below its glass transition. The fitted parameters that describe these processes and the comparison with other polysaccharides allow to ascribe the relaxations to lateral groups on one hand and to local main chain motion on the other hand. Their contributions to the diele...
Article
Full-text available
Glazes for ceramic materials are often opacified by a zircon pigment. The increase in optical density is related to the number and size of pigment particles, which are well dispersed in the glass. Mechanical dispersion by grinding of a fine zircon powder in a raw glaze mixture results in an unoptimised pigment dispersion in the melted glassy phase...
Article
This work is aimed to the production of floor tiles which meet European Standards (BI class), i.e. characterized by a very low water absorption (<3%) and a high strength. First, the physical and chemical characteristics of the raw materials (clay minerals, pyrophylite, feldspar and talc) were studied and the vitrification diagrams were then used to...
Article
Dielectric measurements were performed on concentrated suspensions of TiO2 (50 vol%) and clay (40 vol%) in the frequency range 1 MHz–1 GHz. The suspension rheology was modified by various Tiron or sodium tripolyphosphate additions. Using a specific procedure for data interpretation, it was shown that the global dielectric relaxation phenomenon, in...
Article
Full-text available
Résumé : Dans ce travail, nous nous sommes intéressés à l'étude des propriétés des pâtons après pétrissage. Des études ont montré que les propriétés dynamiques de l'eau influencent le comportement rhéologique et le caractère collant de la pâte. La détermination du comportement viscoélastique de la pâte a été effectuée par analyse thermomécanique dy...

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