Alfonso Prado-Cabrero

Alfonso Prado-Cabrero
Waterford Institute of Technology | WIT · School of Health Sciences

PhD

About

28
Publications
14,469
Reads
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806
Citations
Citations since 2016
16 Research Items
542 Citations
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2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
Introduction
As part of Vistamilk SFI research Centre, I am currently developing a lutein enriched yoghurt to reach the market and help population groups at risk of poor nutrition. More info on my personal website: https://prado-cabrero.com
Additional affiliations
August 2016 - August 2016
Waterford Institute of Technology
Position
  • PostDoc Position
August 2016 - July 2019
Waterford Institute of Technology
Position
  • Research Associate
January 2016 - February 2016
Waterford Institute of Technology
Position
  • Head of Biochemistry Laboratory
Education
October 2000 - December 2006
Universidad de Sevilla
Field of study
  • Fungal Genetics and Biochemistry
October 1995 - June 1999
Universidad de Navarra
Field of study
  • Biochemistry

Publications

Publications (28)
Article
Background: Preliminary work by our center has reported behavior and functional benefits in patients with Alzheimer's disease (AD) following targeted micronutritional supplementation. Objective: To build on the existing exploratory research and investigate the impact of these micronutrients on the natural progression of AD in a randomized contro...
Article
Full-text available
Aquaculture is looking for substitutes for fishmeal and fish oil to maintain its continued growth. Zooplankton is the most nutritious option, but its controlled mass production has not yet been achieved. In this context, we have developed a monoalgal 'green water' closed-loop bioreactor with the microalgae Tetraselmis chui that continuously produce...
Article
Full-text available
Background & Aims Accumulating evidence suggests that omega-3 fatty acids (ω-3FAs), carotenoids and vitamin E can improve cognitive performance. However, their collective impact on cognition has not yet been investigated in healthy individuals. This study investigated the combined effect of ω-3FA, carotenoid and vitamin E supplementation on the cog...
Article
Full-text available
Purpose: The carotenoids lutein (L), zeaxanthin (Z), and meso-zeaxanthin deposit at the macula as macular pigment (MP) and provide visual benefits and protection against macular diseases. The present study investigated MP, its nutritional and environmental determinants, and its constituent carotenoids in serum from a Mexican sample, in healthy par...
Article
Full-text available
The nutraceutical market for EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) is promoting fishing for Euphasia superba (Antarctic krill) in the Southern Ocean and Calanus finmarchicus in Norwegian waters. This industry argues that these species are underexploited, but they are essential in their ecosystems, and climate change is altering...
Article
Full-text available
The harpacticoid copepod Tigriopus californicus has been recognized as a model organism for the study of marine pollutants. Furthermore, the nutritional profile of this copepod is of interest to the aquafeed industry. Part of this interest lies in the fact that Tigriopus produces astaxanthin, an essential carotenoid in salmonid aquaculture. Here, w...
Article
Full-text available
Lutein (L), zeaxanthin (Z), and meso-zeaxanthin (MZ) have been the focus of research and commercial interest for their applications in human health. Research into formulations to enhance their bioavailability is merited. This 6 month randomised placebo-controlled trial involving 81 healthy volunteers compared the bioavailability of different formul...
Article
Full-text available
Omega-3 fatty acids (ω-3FAs), carotenoids, and vitamin E are important constituents of a healthy diet. While they are present in brain tissue, studies have shown that these key nutrients are depleted in individuals with mild cognitive impairment (MCI) in comparison to cognitively healthy individuals. Therefore, it is likely that these individuals w...
Article
Improved life expectancy worldwide has resulted in a significant increase in age-related diseases. Dementia is one of the fastest growing age-related diseases, with 75 million adults globally projected to develop the condition by 2030. Alzheimer's disease (AD) is the most common form of dementia and represents the most significant stage of cognitiv...
Poster
Full-text available
Carotenoids such as astaxanthin and fatty acids such as EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) are used in food supplements and promoted to improve health. The current means of production of astaxanthin (using algae H. pluvialis) is very expensive, and the overexploitation of fish and krill to obtain EPA and DHA represents major...
Article
Full-text available
The macular carotenoids (i.e., lutein (L), zeaxanthin (Z) and meso-zeaxanthin (MZ)) exhibit anti-inflammatory, antioxidant and optical properties that are believed to support human health and function. Studying the accumulation and distribution of these nutrients in tissues and organs, in addition to the eye, is an important step in understanding h...
Article
Full-text available
We previously identified that the concentration of zeaxanthin in some commercially available carotenoid supplements did not agree with the product's label claim. The conclusion of this previous work was that more quality assurance was needed to guarantee concordance between actual and declared concentrations of these nutrients i.e., lutein (L) zeax...
Article
Full-text available
We investigated the concordance between actual and declared content of the three macular carotenoids in commercially available supplements aimed at eye health. Three batches of nine products were tested for content of lutein (L), zeaxanthin (Z) and meso-zeaxanthin (MZ) by chiral HPLC–DAD. In every product tested, actual L concentration was close to...
Article
Full-text available
Significance Saffron is a triploid, sterile species whose red stigmas constitute the most expensive spice on Earth. The color, the taste, and the aroma of the spice are owed to the crocus-specific apocarotenoid accumulation of crocetin/crocins, picrocrocin, and safranal. Through deep transcriptome analysis, we identified a novel carotenoid cleavage...
Article
Full-text available
The presence of hypoxic regions in solid tumors is an adverse prognostic factor for patient outcome. Here, we show that hypoxia induces the expression of Ephrin-A3 through a novel hypoxia-inducible factor (HIF)-mediated mechanism. In response to hypoxia, the coding EFNA3 mRNA levels remained relatively stable, but HIFs drove the expression of previ...
Article
Full-text available
Extracellular signal-regulated kinase 5 (ERK5), also known as big mitogen-activated protein kinase (MAPK) 1, is implicated in a wide range of biologic processes, which include proliferation or vascularization. Here, we show that ERK5 is degraded through the ubiquitin-proteasome system, in a process mediated by the tumor suppressor von Hippel-Lindau...
Article
Full-text available
The orange pigmentation of the ascomycete fungi Neurospora and Fusarium is mainly due to the accumulation of neurosporaxanthin, a carboxylic apocarotenoid whose possible biotechnological applications have not been investigated. From the discovery of the first enzyme of the biosynthetic pathway in 1989, the prenyltransferase AL-3, to the recent iden...
Article
Carotenoids are widespread terpenoid pigments with applications in the food and feed industries. Upon illumination, the gibberellin-producing fungus Fusarium fujikuroi (Gibberella fujikuroi mating population C) develops an orange pigmentation caused by an accumulation of the carboxylic apocarotenoid neurosporaxanthin. The synthesis of this xanthoph...
Article
The synthesis of the acidic apo-carotenoid neurosporaxanthin by the fungus Fusarium fujikuroi depends on four enzyme activities: phytoene synthase and carotene cyclase, encoded by the bifunctional gene carRA, a carotene desaturase, encoded by carB, and a postulated cleaving enzyme converting torulene (C(40)) into neurosporaxanthin (C(35)). Based on...
Article
Full-text available
The car gene cluster of the ascomycete Fusarium fujikuroi encodes two enzymes responsible for torulene biosynthesis (CarRA and CarB), an opsin-like protein (CarO), and a putative carotenoid cleaving enzyme (CarX). It was presumed that CarX catalyzes the formation of the major carotenoid in F. fujikuroi, neurosporaxanthin, a cleavage product of toru...
Article
Full-text available
The ascomycete Fusarium fujikuroi produces carotenoids by means of the enzymes encoded by three car genes. The enzymes encoded by carRA and carB are responsible of the synthesis of beta-carotene and torulene, respectively, while the product encoded by carT cleaves torulene to produce the acidic xanthophyll neurosporaxanthin. carRA and carB are foun...
Article
Opsins are membrane photoreceptors closely related to the heat-shock proteins of the HSP30 family. Their functions include light-driven ion pumping in archaea and light detection in algae and animals, using the apocarotenoid retinal as a light-absorbing prosthetic group. We describe a gene of Fusarium fujikuroi, carO, coding for a polypeptide resem...
Article
The aim of this study was to explore whether IGF-I treatment for two weeks modified fat intestinal absorption, measured from faecal content, in cirrothic and healthy rats. Male Wistar rats were used. Liver cirrhosis was induced by carbon tetrachloride (CCl4) inhalation twice a week for 11 weeks and phenobarbital addition to drinking water (400 mg/l...

Questions

Questions (7)
Question
I'm intending to measure some carotenoids from fish samples, but the carotenoids are in very low concentration. Therefore I have to increase the size of the fish sample, which contains a lot of lipids. I can't inject in the HPLC a such an oily sample, because I can clog the column. I thought in YMC-Pack PVA-Sil, intended to separate lipids, but The HPLC only has a UV detector, I don't have a ELSD. Should be enough the UV detection to locate the lipids in the chromatogram and collect my carotenoid (hopefully free of lipids) and quantify it in a different column? Any other HPLC column suitable for lipid separation?
Thanks!
Question
I have seen that some journals have indexed in pubmed only a small amount of papers. the rest of hundreds of papers of the journal are not indexed. These indexed papers are not free available, so this doesn't seem to be the reason. One example of such a journal is "Journal of Functional Foods". Any clue? Thanks.
Question
The metering head of my Agilent HPLC 1200 is a 900 ul (200 bars). I´m injecting 10 ul of sample only, so I´m wondering whether I have a lot of unnecesary tubing in my system.
In fact, peaks are not well resolved in this HPLC. Could it be due in part to this preparative head? I know that there are much more factors affecting separation, but separation is better in our 1260. I´m afraid that the long 900 ul loop is affecting. I got 2 contradictory advices from Agilent support, so I would appreciate more advice. Thanks.
Question
I have to purchase one for our lab and I want to choose the best in performance-price. I worked previously with Ika and they are good, but I heard that Buchi should be the best. Any advice? Thanks!
Question
 I know that these columns are thought for UHPLC, but If they exist, would it be worthy to use them in a HPLC system providing only 600 bars (Agilent 1260)? Would I get any improvement in my peak separation?
Question
In my spectrophotometer specifications say that the linear spectrophotometric range is 0-3 AU. Then why it can not give an accurate measurement above 1 AU? it gets saturated. I guess spectrophotometers cannot overcome 1 AU. Then why HPLC can reach up to 2 AU linearly? Did I work all my life with bad spectrophotometers?
Question
Given that the sample is pure, containing a quiral molecule

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Projects

Projects (4)
Project
I am currently managing a project funded by the Enterprise Ireland Commercialisation Fund Program to develop a novel biotechnological process aimed to produce natural carotenoids and long chain polyunsaturated fatty acids at the commercial level.