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Alfonso Méndez-Bravo

Alfonso Méndez-Bravo
Universidad Nacional Autónoma de México, ENES Morelia · National Laboratory for Ecological Analysis and Synthesis (LANASE)

PhD

About

45
Publications
23,904
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871
Citations
Citations since 2017
21 Research Items
632 Citations
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2017201820192020202120222023050100150
2017201820192020202120222023050100150

Publications

Publications (45)
Article
Full-text available
Bark is a permanent surface for microbial colonization at the interface of trees and the surrounding air, but little is known about its microbial communities. We used shotgun metagenomic sequencing to analyze the bark microbiomes of avocado trees from two orchards, and compared one of them to rhizospheric soil. It was shown that the microbial commu...
Article
Full-text available
The use of resistant cultivars and fungicides are common methods to control coffee leaf rust (CLR), the main disease that affects the Arabica coffee crop. In this study, we evaluated the response of grafted and ungrafted plants during the early stage of Hemileia vastatrix infection. We used ungrafted plants of Oro Azteca (resistant cultivar) and Ga...
Chapter
Plants interact with a wide range of organisms both above- and belowground that influence each other through nutrient cycling and a wide array of signaling compounds. Plant belowground interactions with soil microbes alter plant fitness and physiology, affecting the performance of plant-associated aboveground organisms through three main pathways:...
Article
The rhizosphere microbiome is critical for plant growth and protection against plant pathogens. However, rhizosphere microbial communities are likely to be restructured upon plant infection by fungal pathogens. Our objective was to determine the shifts in rhizosphere bacterial communities of avocado trees (Persea americana Mill.) after Fusarium die...
Article
Full-text available
The structure and function of rhizosphere microbial communities are affected by the plant health status. In this study, we investigated the effect of root rot on the avocado rhizosphere microbiome, using 16S rDNA and ITS sequencing. Furthermore, we isolated potential fungal pathogens associated with root rot symptoms and assessed their pathogenic a...
Article
Full-text available
Background: Alkamides are plant-specific bioactive molecules. They are low molecular weight N-substituted a-unsaturated acyl amides that display biological explicit activities in different organisms from bacteria, fungi, insects to mammals and plants. The acyl chain has been proposed to be biosynthesized from a fatty acid; however, this has not bee...
Preprint
Bark is a permanent surface for microbial colonization at the interface of trees and the surrounding air. However, little is known about the microbial communities harbored on these tissues. Studies on bark microbial ecology show a dominance of bacteria from a few phyla. Bark microbial communities of avocado (Persea americana) could have implication...
Article
Full-text available
Background In Mexico, coffee leaf rust (CLR) is the main disease that affects the Arabica coffee crop. In this study, the local response of two Mexican cultivars of Coffea arabica (Oro Azteca and Garnica) in the early stages of Hemileia vastatrix infection was evaluated. Methods We quantified the development of fungal structures in locally-infecte...
Conference Paper
Full-text available
El efecto que tienen los portainjertos en los injertos para resistir la infección de patógenos ha demostrado que la severidad de la infección puede disminuir o aumentar dependiendo del portainjerto. En un estudio previo se evaluaron los cambios en la expresión de genes de defensa en plantas injertadas de café para identificar sí el uso de portainje...
Poster
The Korushio Shot Hole Borer Euwallacea kuroshio (KSHB) is an invasive pest coming from Asia which is rapidly expanding in California. KSHB is associated with several symbiotic fungi, one of them from the genus Fusarium that causes a vascular disease known as Fusarium Dieback (FD) [1]–[4]. FD affects more than 300 host plant species, including avoc...
Article
Full-text available
The avocado, Persea americana , is a fruit crop of immense importance to Mexican agriculture with an increasing demand worldwide. Avocado lies in the anciently diverged magnoliid clade of angiosperms, which has a controversial phylogenetic position relative to eudicots and monocots. We sequenced the nuclear genomes of the Mexican avocado race, P. a...
Preprint
Full-text available
The avocado, Persea americana , is a fruit crop of immense importance to Mexican agriculture with an increasing demand worldwide. Avocado lies in the anciently-diverged magnoliid clade of angiosperms, which has a controversial phylogenetic position relative to eudicots and monocots. We sequenced the nuclear genomes of the Mexican avocado race, P. a...
Article
Recent studies showed that bacterial volatile organic compounds (VOCs) play an important role in the suppression of phytopathogens. The ability of VOCs produced by avocado (Persea americana Mill.) rhizobacteria to suppress the growth of common avocado pathogens was therefore investigated. We evaluated the antifungal activity of VOCs emitted by avoc...
Article
Full-text available
Plant rhizobacteria have been successfully used as biocontrol agents against fungal phytopathogens. However, their potential to control two important avocado diseases, namely Fusarium dieback (FD) and Phytophthora root rot (PRR), has been poorly studied. FD is an emerging disease triggered by fungi associated with two ambrosia beetle species (Euwal...
Article
Full-text available
Rhizobacteria associated with crops constitute an important source of potentially beneficial microorganisms with plant growth promoting activity or antagonistic effects against phytopathogens. In this study, we evaluated the plant growth promoting activity of 11 bacterial isolates that were obtained from the rhizosphere of healthy avocado trees and...
Data
Bacterial isolates obtained from avocado rhizosphere and effect on Arabidopsis thaliana seedlings. †The effect of rhizobacteria isolated from avocado symptomatic trees (A) or healthy trees (B) on Arabidopsis seedlings in vitro at seventh day after inoculation. The effects were divided into three categories; Positive, visible growth promotion effect...
Data
Co-cultivation of Arabidopsis thaliana seedlings with rhizobacterial isolates from healthy non-symptomatic avocado trees. Representative photographs of Arabidopsis Col-0 seedlings inoculated with rhizobacterial isolates at 2.5 cm (long distance) and 1 cm (close distance) from root tip. Primary root length (b), lateral root number (c) and fresh weig...
Data
Representative total ion chromatogram of volatile emissions by PGPR isolate A8a. The volatile profile of the control sample (LB agar medium; a) was contrasted with the profile of volatiles emitted by isolate A8a (b). Arrows indicate differential peaks of compounds described in Table 3. (TIF)
Poster
Full-text available
The Polyphagous Shot Hole Borer and the Kuroshio Shot Hole Borer (Euwallacea spp. nr. fornicatus) are invasive beetles that vector a disease called Fusarium Dieback, caused by fungi that the beetles inoculate inside host tissue and utilize as a nutrition source. Fusarium dieback affects avocado (Persea americana Mill.) and many other tree species t...
Poster
Poster - ”Cambios en la microbiota rizosférica del aguacate causados por la marchitez por Fusarium y búsqueda de agentes de control biológico” - IV Simposio Nacional de Biotecnología para una Agricultura Sustentable - Diciembre 2016, Morelia, Michoacán.
Poster
Poster - ”Cambios en la microbiota rizosférica del aguacate causados por la marchitez por Fusarium y búsqueda de agentes de control biológico” - IV Simposio Nacional de Biotecnología para una Agricultura Sustentable - Diciembre 2016, Morelia, Michoacán.
Poster
Poster - ”Cambios en la microbiota rizosférica del aguacate causados por la marchitez por Fusarium y búsqueda de agentes de control biológico” - IV Simposio Nacional de Biotecnología para una Agricultura Sustentable - Diciembre 2016, Morelia, Michoacán.
Poster
Full-text available
Poster - ”Cambios en la microbiota rizosférica del aguacate causados por la marchitez por Fusarium y búsqueda de agentes de control biológico” - IV Simposio Nacional de Biotecnología para una Agricultura Sustentable - Diciembre 2016, Morelia, Michoacán.
Article
Full-text available
Avocado (Persea americana) is an economically important tropical fruit considered to be a good source of fatty acids. Despite its importance, the molecular and cellular characterization of biochemical and developmental processes in avocado is limited due to the lack of transcriptome and genomic information. The transcriptomes of seeds, roots, stems...
Article
Full-text available
Soil contamination by hexavalent chromium [Cr(VI) or chromate] due to anthropogenic activities has become an increasingly important environmental problem. To date few studies have been performed to elucidate the signaling networks involved on adaptive responses to (CrVI) toxicity in plants. In this work, we report that depending upon its concentrat...
Article
Full-text available
Hydroponics is a plant growth system that provides a more precise control of growth media composition. Several hydroponic systems have been reported for Arabidopsis and other model plants. The ease of system set up, cost of the growth system and flexibility to characterize and harvest plant material are features continually improved in new hydropon...
Article
Full-text available
Pyocyanin acts as a virulence factor in Pseudomonas aeruginosa, a plant and animal pathogen. In this study we evaluated the effect of pyocyanin on growth and development of Arabidopsis seedlings. Root inoculation with P. aeruginosa PAO1 strain inhibited primary root growth in WT Arabidopsis seedlings. In contrast, single lasI- and double rhlI-/lasI...
Data
Full-text available
Functional categories over-represented in N -isobutyl decanamide responsive genes. (PDF)
Data
Effect of N-isobutyl decanamide on Botrytis cinerea mycelial growth. B. cinerea mycelium excised from a solid culture in Petri dishes was transferred to potato dextrose agar dishes supplemented with N-isobutyl decanamide at the concentrations indicated. Radial growth of the fungus was measured 24, 48 and 72 h after inoculation (A). Data means avera...
Data
Experimental design for microarray analysis. 6 day-old Arabidopsis Col-0 seedlings were grown on N-isobutyl decanamide-free medium and then transferred to control medium (A) supplied with the solvent, or to 60 µM N-isobutyl decanamide-containing medium (B). Pictures were taken 14 days after transfer (d.a.t.). Modified loop design including 4 indepe...
Data
Validation of microarray results via qRT-PCR. Quantitative real-time PCR analysis was performed for 15 genes in Arabidopsis (Col-0) seedlings, under the same conditions used for microarray analysis (1, 3, 7 and 14 days of treatment with 60 µM N-isobutyl decanamide). Fold-change (control to N-isobutyl-decanamide) expression for the indicated selecte...
Data
Local induction of defense genes by N-isobutyl decanamide on detached leaves. Leaves from 20 day-old transgenic LOX2∶GUS or WT (Col-0) plants grown in soil were detached and incubated 24 h on solvent (control, white squares), or 30 µM N-isobutyl decanamide containing plates (black squares), transferred to decanamide-free plates and then analized. (...
Data
Full-text available
Full list of genes differentially expressed by N -isobutyl decanamide in Arabidopsis . (PDF)
Data
Full-text available
Sequences of oligonucleotides used as PCR primers for quantitative expression analysis. (PDF)
Article
Full-text available
Alkamides are fatty acid amides of wide distribution in plants, structurally related to N-acyl-L-homoserine lactones (AHLs) from Gram-negative bacteria and to N- acylethanolamines (NAEs) from plants and mammals. Global analysis of gene expression changes in Arabidopsis thaliana in response to N-isobutyl decanamide, the most highly active alkamide i...
Article
Full-text available
Serotonin (5-hydroxytryptamine) is a well-known neurotransmitter in mammals and is widely distributed in plants. This compound is synthesized from tryptophan and shares structural similarity with IAA. To date, little is known about the morphological, physiological and molecular responses of plants to serotonin. In this study, we characterized the e...
Article
Full-text available
Alkamides are small bioactive lipid signals with a wide distribution in plants. In this report, the role of nitric oxide (NO) in the alterations induced by N-isobutyl decanamide on the Arabidopsis (Arabidopsis thaliana) root system architecture (RSA) was investigated. We first compared the effects of N-isobutyl decanamide and NO donors sodium nitro...
Article
Full-text available
Alkamides belong to a class of small lipid signals of wide distribution in plants, which are structurally related to the bacterial quorum-sensing signals N-acyl-l-homoserine lactones. Arabidopsis (Arabidopsis thaliana) seedlings display a number of root developmental responses to alkamides, including primary root growth inhibition and greater forma...
Chapter
Plant growth and development are driven by the integration of a vast number of signals including volatile compounds, small organic molecules, peptides, steroids and lipids. Among these, amino-containing signalling lipids such as alkamides and N-acyl ethanolamines (NAEs) have emerged as important regulators of cellular processes, including cell prol...
Article
Full-text available
Alkamides and N-acylethanolamines represent a new class of lipid compounds related to animal endocannabinoids, which regulate different aspects of plant morphogenesis. To elucidate further the plant regenerative properties of alkamides and their role on plant development, we used an in vitro system to cultivate Arabidopsis thaliana explants under v...
Article
Full-text available
Alkamides and N-acilethanolamides are a class of lipid compounds related to animal endocannabinoids of wide distribution in plants. We investigated the structural features required for alkamides to regulate plant development by comparing the root responses of Arabidopsis (Arabidopsis thaliana) seedlings to a range of natural and synthetic compounds...
Conference Paper
Avocado (Persea americana) is one of the rare crop plants among the basal angiosperms that belong to Lauracea family. Genomic characterization of avocado will provide an opportunity to examine the developmental genetics of fleshy fruits and some of the mechanisms of early evolutive adaptations in flowering plants. Avocado is also a product of great...
Conference Paper
Avocado (Persea americana) is a major fruit crop with high nutritional and industrial value. It is one of the rare crop plants among the basal angiosperms that belongs to Lauraceae family. Genomic characterization of avocado will provide an opportunity to examine the developmental genetics of fleshy fruits and some of the mechanisms of early evolut...

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Projects

Projects (3)
Project
Predict metabolic pathways of avocado involved in Fusarium dieback response from transcriptomic data from California trees infected with Fusarium kuroshium.
Project
We are evaluating bacterial strains that we isolated from diverse habitats (soil, roots, leaves and barks of different plant species) for antagonistic activity against important fungal pathogens. We are also determining the volatiles and diffusible compounds that are emitted by our bacterial strains of interest.
Project
This project aims at unravelling how plant pathogens influence the taxonomic and functional diversity of rhizosphere bacterial communities. Microbial communities associated with the plant rhizosphere are critical for plant growth and productivity, nutrient cycling and other ecosystem processes. Any alteration in their structure and diversity could thus affect soil and plant quality. We are also looking at isolating rhizobacteria with antifungal activity and at identifying the bacterial chemical compounds that are responsible for the antagonism.