Alfonso Mate- PhD
- Professor (Full) at University of Seville
Alfonso Mate
- PhD
- Professor (Full) at University of Seville
About
84
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Introduction
Current institution
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January 2010 - December 2012
Publications
Publications (84)
Arterial hypertension leads to oxidative and inflammatory imbalances, triggering hypertensive organ damage through several pathways. We have previously described the antioxidant and anti‐inflammatory properties of olive oil extracted from the wild olive tree (Olea europaea var. sylvestris, acebuche, ACE) against hypertensive ocular damage. The aim...
Glaucoma is characterized by cupping of the optic disc, apoptotic degeneration of retinal ganglion cells (RGCs) and their axons, and thinning of the retinal nerve fiber layer, with patchy loss of vision. Elevated intraocular pressure (IOP) is a major risk factor for hypertensive glaucoma and the only modifiable one. There is a need to find novel co...
Aims/Purpose: Many systemic and ocular disturbances are closely related to oxidative stress, including hypertensive eye diseases. In this context, the oral administration of wild olive oil, also known as acebuche (ACE) oil ( Olea europaea var. sylvestris ), proved to modulate local production of reactive oxygen species (ROS) by the enzyme nicotinam...
Aims/Purpose: Arterial hypertension (AH) leads to oxidative and inflammatory imbalance triggering hypertensive organ damage via different pathways. Oil extracted from the wild olive tree (acebuche, ACE) has previously shown antioxidant and anti‐inflammatory properties against hypertensive ocular damage when regularly consumed in the diet 1,2 . We t...
Scope
Our laboratory has previously described the antioxidant and anti‐inflammatory potential of a wild olive (acebuche, ACE) oil against hypertension‐associated vascular retinopathies. The current study aims to analyze the antifibrotic effect of ACE oil on the retina of hypertensive mice.
Methods and results
Mice are rendered hypertensive by admi...
Este recurso forma parte del Proyecto “Aplicación de realidad virtual sobre el laboratorio de prácticas de Fisiología del Sistema Visual” (“Apoyo al diseño de recursos docentes basados en las nuevas tecnologías” correspondiente a la Línea de actuación Actividades del profesorado para la innovación educativa ref. 222 del IV Plan Propio de Docencia d...
Este recurso forma parte del Proyecto “Aplicación de realidad virtual sobre el laboratorio de prácticas de Fisiología del Sistema Visual” (“Apoyo al diseño de recursos docentes basados en las nuevas tecnologías” correspondiente a la Línea de actuación Actividades del profesorado para la innovación educativa ref. 222 del IV Plan Propio de Docencia d...
Este recurso forma parte del Proyecto “Aplicación de realidad virtual sobre el laboratorio de prácticas de Fisiología del Sistema Visual” (“Apoyo al diseño de recursos docentes basados en las nuevas tecnologías” correspondiente a la Línea de actuación Actividades del profesorado para la innovación educativa ref. 222 del IV Plan Propio de Docencia d...
Este recurso forma parte del Proyecto “Laboratorio de prácticas de Fisiología del Sistema Visual” del III Plan Propio de Docencia de la Universidad de Sevilla (Ref. 1.2.1 Dotación y mejora de recursos para la docencia (Recursos Audiovisuales), conv. 2021-2022) donde se produce material audiovisual (vídeo didáctico) del laboratorio de prácticas prin...
Este recurso forma parte del Proyecto “Laboratorio de prácticas de Fisiología del Sistema Visual” del III Plan Propio de Docencia de la Universidad de Sevilla (Ref. 1.2.1 Dotación y mejora de recursos para la docencia (Recursos Audiovisuales), conv. 2021-2022) donde se produce material audiovisual (vídeo didáctico) del laboratorio de prácticas prin...
Este recurso forma parte del Proyecto “Laboratorio de prácticas de Fisiología del Sistema Visual” del III Plan Propio de Docencia de la Universidad de Sevilla (Ref. 1.2.1 Dotación y mejora de recursos para la docencia (Recursos Audiovisuales), conv. 2021-2022) donde se produce material audiovisual (vídeo didáctico) del laboratorio de prácticas prin...
El recurso audiovisual tiene como objetivo profundizar en los conceptos teórico-prácticos de electrofisiología ocular impartidos en las asignaturas mencionadas. En el vídeo se muestra la realización de pruebas electrofisiológicas avanzadas para el estudio del sistema visual, como el registro de potenciales evocados visuales, de los movimientos ocul...
Targeted therapies with antiangiogenic drugs (e.g., sunitinib) and immune checkpoint inhibitors (e.g., anti-PD-1 antibodies) are the standard of care for patients with metastatic renal cell carcinoma. Although these treatments improve patient survival, they are rarely curative. We previously hypothesized that advanced cancers might be treated witho...
Preeclampsia (PE) is a pregnancy-specific disorder characterized by the new onset of hypertension plus proteinuria and/or end-organ dysfunction. Here, we investigate the role of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system as a major component of reactive oxygen species generation, in a rodent model of early-onset preeclam...
Targeted therapies with antiangiogenic drugs (e.g., sunitinib) and immune checkpoint inhibitors (e.g., anti-PD-1 antibodies) are the standard of care for patients with metastatic renal cell carcinoma. Although these treatments improve patient survival, they are rarely curative. We previously hypothesized that advanced cancers might be treated witho...
Arterial hypertension (AH) leads to oxidative and inflammatory imbalance that contribute to fibrosis development in many target organs. Here we aimed to highlight the harmful effects of severe AH in the cornea. Our experimental model was established by administration of NG-nitro-L-arginine-methyl-ester (L-NAME) to C57BL/6 mice, which were monitored...
Purpose
Despite numerous reports on the beneficial effects of olive oil in the cardiovascular context, very little is known about the olive tree’s wild counterpart ( Olea europaea , L. var. sylvestris ), commonly known as acebuche (ACE) in Spain. The aim of this study was to analyse the possible beneficial effects of an extra virgin ACE oil on vasc...
Para nuestro proyecto de “Canal virtual de divulgación sobre Fisiología” se ha utilizado diferentes redes sociales como son Instagram y YouTube, donde se han ido publicando diferentes contenidos sobre Fisiología. Los contenidos subidos a las redes sociales de manera periódica han sido creados por los estudiantes que cursan asignaturas en el Departa...
Inflammation plays a crucial role in the course of eye diseases, including many vascular retinopathies. Although olive oil is known to have beneficial effects against inflammatory processes, there is no information available on the anti-inflammatory potential of the wild olive tree (namely, acebuche (ACE) for the primitive Spanish lineages). Here w...
Other than being a physiological process, pregnancy is a condition characterized by major adaptations of maternal endocrine and metabolic homeostasis that are necessary to accommodate the fetoplacental unit. Unfortunately, all these systemic, cellular, and molecular changes in maternal physiology also make the mother and the fetus more prone to adv...
Purpose:
Increased reactive oxygen species (ROS) released by NADPH oxidase and inflammation are associated with arterial hypertension and eye diseases associated with high blood pressure, including glaucoma, retinopathies (e.g., age-related macular degeneration), and choroidopathies affecting ocular function; however, the mechanisms underlying the...
Background
Echinomycin (EKN), an inhibitor of hypoxia-inducible factor (HIF)-1 DNA-binding activity, has been implied as a possible therapeutic agent in ischemic diseases. Here, we assess EKN in hypoxia-driven responses in vitro using human primary adult retinal pigment epithelium cells (aRPE) and retinal endothelial cells (hREC), and in vivo using...
Healthy lifestyle habits spanning from preconception to postpartum are considered as a major safeguard for achieving successful pregnancies and for prevention of gestational diseases. Among preconception priorities established by the World Health Organization (WHO) are healthy diet and nutrition, weight management, physical activity, planned pregna...
Late-onset preeclampsia (LOPE) associates with reduced umbilical vein reactivity and endothelial nitric oxide synthase (eNOS) activity but increased human cationic amino acid (hCAT-1)–mediated L-arginine transport involving A2A adenosine receptor in the fetoplacental unit. This study addresses the A2B adenosine receptor (A2BAR)-mediated response to...
Oxidative stress plays an important role in the pathogenesis of ocular diseases, including hypertensive eye diseases. The beneficial effects of olive oil on cardiovascular diseases might rely on minor constituents. Currently, very little is known about the chemical composition and/or therapeutic effects of the cultivated olive tree's counterpart, w...
Sunitinib (Su) is a tyrosine kinase inhibitor with antiangiogenic and antineoplastic effects that is recommended therapy for renal cell carcinoma, gastrointestinal stromal tumors, and pancreatic neuroendocrine tumors. Arterial hypertension is one of the adverse effects observed in the treatment with Su. The aim of this work was to deepen our unders...
Purpose
Echinomycin (EKN), an inhibitor of hypoxia‐inducible factor (HIF)‐1 DNA‐binding activity, has been implied as a possible therapeutic agent in ischemic diseases. We assessed EKN in hypoxia‐driven responses in vitro using human primary adult retinal pigment epithelium cells (aRPE), and in vivo using the laser‐induced mouse choroidal neovascul...
The role of oxidative stress in the physiopathology of human pregnancy is of particular interest. Pregnancy is well-known to increase the oxidative stress, mainly produced by a normal systemic inflammatory response, which results in high amounts of circulating reactive oxygen species (ROS) and reactive nitrogen species (RNS). Both ROS and RNS play...
Pregnancy hypertensive disorders such as preeclampsia (PE) are strongly correlated with insulin resistance, a condition in which the metabolic handling of D-glucose is deficient. In addition, the impact of preeclampsia is enhanced by other insulin resistant disorders, including polycystic ovary syndrome and obesity. For this reason, there is a clea...
Intercellular communication is a critical process in biological mechanisms. During pregnancy foetoplacental tissues release a heterogeneous group of extracellular vesicles (EVs) that include exosomes, microvesicles, apoptotic bodies, and syncytial nuclear aggregates. These vesicles contain a complex cargo (proteins, DNA, mRNA transcripts, microRNAs...
L-carnitine (LC) exerts beneficial effects in arterial hypertension due, in part, to its antioxidant capacity. We investigated the signalling pathways involved in the effect of LC on angiotensin II (Ang II)-induced NADPH oxidase activation in NRK-52E cells. Ang II increased the generation of superoxide anion from NADPH oxidase, as well as the amoun...
Adenosine is an endogenous nucleoside with pleiotropic effects in different physiological processes including circulation, renal blood flow, immune function, or glucose homeostasis. Changes in adenosine membrane transporters, adenosine receptors, and corresponding intracellular signalling network associate with development of pathologies of pregnan...
Leptin is a protein involved in the regulation of food intake and in the immune and inflammatory responses, among other functions. Evidences demonstrate that obesity is directly associated with high levels of leptin, suggesting that leptin may directly link obesity with the elevated cardiovascular and renal risk associated with increased body weigh...
Sunitinib (Su) is currently approved for treatment of several malignances. However, along with the benefits of disease stabilization, cardiovascular toxicities have also been increasingly recognized. The aim of this study was to analyze which mechanisms are involved in the cardiotoxicity caused by Su, as well as to explore the potential cardioprote...
Preeclampsia is associated with impaired placental vasodilation and reduced endothelial nitric oxide synthase (eNOS) activity in the foetoplacental circulation. Adenosine and insulin stimulate vasodilation in endothelial cells, and this activity is mediated by adenosine receptor activation in uncomplicated pregnancies; however, this activity has ye...
Although clays are wildly used in a range of applications, the toxicity assessment of these new materials is still scarce. In the present study, oxidative stress induced by Clay 1, a novel clay, was determined in rats after 90 d of oral exposure. The activities of antioxidant enzymes, namely, superoxide dismutase (SOD), catalase (CAT), glutathione...
Impaired L-carnitine uptake correlates with higher blood pressure in adult men, and L-carnitine restores endothelial function in aortic rings from spontaneously hypertensive rat (SHR). Thus, endothelial dysfunction in hypertension could result from lower L-carnitine transport in this cell type. L-Carnitine transport is mainly mediated by novel orga...
The development of renal fibrosis is a consequence of arterial hypertension. L-carnitine plays an essential role in the β-oxidation of fatty acids, and we have previously demonstrated hypotensive, antioxidant, and anti-inflammatory effects of L-carnitine in arterial hypertension. This work aims to analyze the effect of L-carnitine on renal fibrosis...
Cardiac fibrosis is a pathogenic factor in a variety of cardiovascular diseases and is characterized by an abnormal accumulation of extracellular matrix protein that leads to cardiac dysfunction. L-carnitine (LC) plays an essential role in the β-oxidation of long-chain fatty acids in lipid metabolism. We have previously demonstrated the beneficial...
Purpose:
Arterial hypertension is associated with a high production of reactive oxygen species and a decrease in the antioxidant defense systems. Based on the lack of toxicity of L-carnitine (LC) and previous studies reporting beneficial effects of this compound in experimental models of hypertension, the aim of this work was to test the hypothesi...
Highlights
► Drug discovery in the field of preeclampsia is particularly sensitive. ► The pathophysiology of preeclampsia remains unknown. ► The discovery of possible common effector pathways is crucial ► Adenosine–l-arginine–nitric oxide pathway may be a preferential target ► l-Carnitine may have a relevant role in preeclampsia prevention.
Pregnan...
Preeclampsia is a syndrome that affects a significant proportion of pregnant women worldwide. The several hypotheses proposed regarding the aetiology of preeclampsia highlight the possibility of a critical role played by the placenta and the multiple molecules released by this organ in the onset of the syndrome. Recent reports suggest that not only...
In spite of a wide range of drugs being available in the market, treatment of arterial hypertension still remains a challenge, and new therapeutic strategies could be developed in order to improve the rate of success in controlling this disease. Since oxidative stress has gained importance in the last few years as one of the mechanisms involved in...
Captopril is an angiotensin-converting enzyme (ACE) inhibitor widely used in the treatment of arterial hypertension and cardiovascular diseases. Our objective was to study whether captopril is able to attenuate the cardiac inflammatory process associated with arterial hypertension.
Left ventricle mRNA expression and plasma levels of pro-inflammator...
It has been shown that oxidative stress is involved in the pathogenesis of arterial hypertension. The aim of this work was to study and compare the molecular mechanisms of the antioxidant properties of l-carnitine and captopril in spontaneously hypertensive rats (SHR). Antioxidant enzyme activity/regulation (glutathione peroxidase, glutathione redu...
Subclinical organ damage is a very important aspect when assessing total cardiovascular risk in hypertensive subjects. Therapeutic strategies in those patients should consider treatment of hypertension-related cardiovascular and renal damage in addition to achieving the recommended blood pressure targets. l-carnitine (LC) is a naturally occurring c...
La Facultad de Farmacia de la Universidad de Sevilla (US) tiene en marcha un Programa de Alumnos Tutores desde 2006/07 con el objetivo de que alumnos de cursos superiores (AATT) tutelen a alumnos de nuevo ingreso (1x3). Pretende generar una actitud responsable en los AATT y favorecerles el desarrollo de habilidades sociales, objetivos cualitativos...
El objetivo principal de este trabajo es fomentar el autoaprendizaje y la relación profesor-alumno. Esto lo conseguiremos promoviendo el seguimiento continuado del alumno mediante tutorías presenciales y virtuales, y la evaluación de varios temas del programa seleccionados por el profesor para ser abordados por los alumnos. La materia elegida, Hema...
The main aim of this project is to stimulate the creation of self-learning material and to improve the relationship between teachers and students. This will be achieved through the construction of a lesson of the theoretical program, by the student him- or herself. Part of the Pharmacy Degree course, Haematology, was the chosen subject. This work h...
The mechanism(s) underlying the effects of L-carnitine (beta-hydroxy-gamma-N-trimethylammonium-butyrate; LC) in cardiovascular diseases are not well clarified. Previous studies have demonstrated that oxidative stress and inflammation contribute to arterial hypertension, and antioxidant and/or anti-inflammatory therapies have been proposed. We hypot...
The present study aimed to investigate whether l-carnitine (LC) protects the vascular endothelium and tissues against oxidative damage in hypertension. Antioxidant enzyme activities, glutathione and lipid peroxidation were measured in the liver and heart of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. Nitrite and nitrate level...
Experimental models of hypertension, such as spontaneously hypertensive rats (SHR), show alterations in cellular sodium transport that affects Na(+)-coupled cotransport processes and has been involved in the pathogenesis of this disease. The objective of the present study was to analyze the kinetic properties of the sodium-dependent glucose transpo...
Oxidative stress plays an important role in arterial hypertension and propionyl-L-carnitine (PLC) has been found to protect cells from toxic reactive oxygen species. In this work, we have evaluated the antioxidant capacity of chronic PLC treatment in spontaneously hypertensive rats (SHR) by measuring the activity of antioxidant enzymes and the lipi...
Hepatocytes and cholangiocytes release ATP into bile, where it is rapidly degraded into adenosine and P(i). In rat, biliary P(i) concentration (0.01 mM) is approximately 100-fold and 200-fold lower than in hepatocytes and plasma, respectively, indicating active reabsorption of biliary P(i). We aimed to functionally characterize canalicular P(i) rea...
IntroducciónEl trabajo trata de evaluar la acción antioxidante de la propionil-L-carnitina (PLC) en el hígado y corazón de ratas hipertensas (spontaneusly hypertensive rats [SHR]) y normotensas (Wistar-Kyoto [WKY]). Además, estudiamos su acción hipolipidemiante.Material y métodosRatas WKY y SHR de 4 semanas se tartan con PLC (200 mg/kg de peso corp...
The effect of acute exposure of intraperitoneal injection of microcystin-LR (MCLR) on antioxidant enzymes and lipid peroxidation has been studied in liver and kidney of rats. Rats were treated with two doses, i.e. 100 and 150 microg of pure MCLR/kg body weight or saline solution. The enzyme activities of glutathione peroxidase (GSH-Px), glutathione...
Abnormalities in carbohydrate metabolism and the insulin resistance status have been associated with hypertension. We have previously described alterations in the sodium-coupled sugar absorption in an experimental model of hypertension; in the present work, we studied the regulation of the sodium-independent, GLUT5-facilitated D-fructose intestinal...
The objective of the present study was to evaluate the effects of microcystin-LR (MCLR) on the activity of membrane enzymes from intestinal mucosa. In addition, serum chemistry and peroxidative status of both serum and intestinal homogenate were evaluated after treatment with MCLR. Wistar rats were treated with intraperitoneal injection of either 1...
D-glucose absorption, distinguishing between active and diffusive components, was studied in the ileum of spontaneously hypertensive rats (SHR) and their normotensive control Wistar-Kyoto (WKY) rats. Net water transport was also determined.
A perfusion system in vivo with ileum loops was used, and experiments with phlorizin, phloretin and 2,4,6-tri...
To examine the effect of long-term administration of L-carnitine on L-carnitine transport in renal brush-border membrane vesicles (BBMVs) from normotensive, Wistar-Kyoto rats.
Rats (n = 20) were orally administered 0.2 g carnitine/kg body weight per day for a total period of 8 weeks. Kinetic parameters of L-carnitine uptake were calculated by non-l...
D-fructose transport was characterized in renal brush-border membrane vesicles (BBMVs) from both spontaneously hypertensive rats (SHR) and normotensive genetic control Wistar-Kyoto (WKY) rats. Kinetic studies indicated that the maximal rate (Vmax) of D-fructose transport was significantly lower in SHR compared with WKY rats. No differences were obs...
The lipid composition and fluidity in brush-border membrane vesicles (BBMV) isolated from renal cortex of spontaneously hypertensive rats (SHR) and their normotensive control, Wistar Kyoto (WKY) rats have been studied. The activity of Na+-dependent D-glucose transport has been also determined. A significant increase in total phospholipids and free...
Na+-dependent D-glucose and D-galactose transport was studied in the ileal brush-border membrane vesicles from both spontaneously hypertensive rats (SHR) and their normotensive genetic control Wistar-Kyoto (WKY) rats. Initial rates and accumulation ratios of the transport of both monosaccharides were significantly lower in the hypertensive rats com...
Ultrastructural studies on the epithelium, sugar transport and immunocytochemistry of Na+-glucose cotransporter (SGLT1) were carried out in the jejunum of Spontaneously Hypertensive Rats (SHR) and their normotensive genetic control, Wistar-Kyoto (WKY) rats. Electron microscopy studies showed a regular brush-border membrane in the jejunal enterocyte...
Na(+)-dependent D-glucose and D-galactose transport were studied in brush-border membrane vesicles (BBMVs) from kidney cortex isolated from both spontaneously hypertensive rats (SHR) and their normotensive genetic control Wistar-Kyoto (WKY) rats. Initial rates and accumulation ratios of Na(+)-dependent D-glucose and D-galactose transport were signi...
En: Revista de Enseñanza Universitaria Sevilla 2001, n. 18 ; p. 33-45 El presente proyecto ha tenido por objeto el diseño y la elaboración de un módulo práctico de aprendizaje que permita a estudiantes de Toxicologa de la Universidad de Sevilla, tanto presenciales como virtuales y a toxicólogos establecidos, el acceso a diferentes tipos de fuentes...
