Alexis Parenté

Alexis Parenté
University of Abomey-Calavi | UAC · Faculty of Health Science (FSS)

PhD Biological & Health Sciences
Postdoctoral researcher (Molecular Genetics in Human and animal Health). Contact : alexis.parente@unilim.fr

About

20
Publications
2,640
Reads
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50
Citations
Citations since 2017
10 Research Items
49 Citations
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2017201820192020202120222023051015
Introduction
PhD Molecular and Cell Biology - PostDoctoral Researcher - Laboratory of Chronic and Neurologic Diseases Epidemiology - Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin. Focusing on Genetic Risk Factors for Breast Cancer in Benin. Contact : alexis.parente@unilim.fr
Additional affiliations
July 2021 - present
University of Abomey-Calavi
Position
  • PostDoc Position
October 2019 - July 2021
University of Limoges
Position
  • Lecturer
October 2015 - September 2019
University of Limoges
Position
  • PhD Student

Publications

Publications (20)
Article
Full-text available
Background GASP-2 is a secreted multi-domain glycoprotein known as a specific inhibitor of myostatin and GDF-11. Here we investigate the role of GASP-2 on myogenesis and the effect of its glycosylation on its activity. Methods GASP-2 overexpression or knockdown by shRNAs were carried out on C2C12 myoblasts cells. In silico analysis of GASP-2 prote...
Article
Full-text available
Background/aims: Myostatin is known as a powerful negative regulator of muscle growth playing a key role in skeletal muscle homeostasis. Recent studies revealed that myostatin-deficient mice lead to an increase of insulin sensitivity, a decrease of adiposity and a resistance to obesity, showing that myostatin can also impact on metabolism. Thus, m...
Article
The transcriptional co-activator PGC-1α has been reported to play a key role in adaptive thermogenesis and to influence muscle homeostasis and growth in mouse and human. PGC-1α has a complex structure with multiple protein domains whose gene is controlled by two promoters and is subject to alternative splicing events. In cattle, very little is curr...
Chapter
Full-text available
Myogenesis is a multistep process taking place during pre- and postnatal stages for muscle formation, growth, and regeneration. It is a highly regulated process involving many molecular factors which act during myoblast proliferation and differentiation. To provide new insights into the molecular mechanisms and interactions behind the regulation of...
Article
Full-text available
PhexL222P mouse is a new ENU mouse model for XLH disease due to Leu to Pro amino acid modification at position 222. PhexL222P mouse is characterized by growth retardation, hypophosphatemia, hypocalcemia, reduced body bone length, and increased epiphyseal growth plate thickness and femur diameter despite the increase in PHEXL222P expression. Actuall...
Conference Paper
Nous présentons une nouvelle stratégie instrumentale et computationnelle appelée FAMOUS (pour fast algorithm for three-dimensional (3D) multiphoton microscopy of biomedical structures) basée sur une approche de microscopie multiphotonique assistée par calcul. Le but est l’amélioration visuelle des images d'échantillons biologiques épais offrant ain...
Article
Full-text available
We present a new instrumental and computational pipeline named FAMOUS: f ast a lgorithm for three-dimensional (3D) m ultiphoton microscopy of bi o medical str u cture s . This pipeline rests on a MPM strategy combined with an original 3D post-processing computational approach. In the present work, FAMOUS approach is devoted to the 3D imaging of the...
Article
Full-text available
Muscle atrophy is associated with many diseases including genetic disorders, sarcopenia, or cachexia syndromes. Myostatin (Mstn), a transforming growth factor-beta (TGF-β) member, plays a key role in skeletal muscle homeostasis as a powerful negative regulator. Over the last decade, about 15 clinical trials aimed at inhibiting the Mstn pathway, fai...
Article
Full-text available
While GASP‐1 and GASP‐2 proteins are known to regulate myogenesis by inhibiting myostatin, their structural organization suggests a putative role as multivalent protease inhibitors controlling different protease activities. In this study, we show the non‐competitive and competitive anti‐trypsin activities of the full‐length GASP‐1 and GASP‐2 protei...
Thesis
GASP-1 and GASP-2 are two closely related proteins structurally characterized by several protease inhibitor modules and identified as inhibitors of several members of the TGF-ß family such as myostatin or GDF-11, negative regulators of myogenesis and osteogenesis, respectively. Despite the common structural organization of GASPs proteins, their dif...
Poster
Myostatin (Mstn) is a negative regulator of muscle growth and allows to control the balance between muscle hypertrophy and atrophy. As in many muscle diseases, this balance is altered. Knockout Mstn-/- mice exhibit a hypermuscular phenotype characterized by an hyperplasia and hypertrophy of muscle fibers and a decrease in fat mass. One of the lines...

Questions

Questions (3)
Question
Hello,
We wish to electroporate ES cells with protein nickase D10A and two sgRNAs targeting our gene of interest.
Before adding the RNP complexes to the electroporation cuvette containing our cells :
Should the RNP complexes be formed independently? (1 tube with nickase D10A + sgRNA1 and 1 tube with nickase D10A + sgRNA-2) or mix the 2 sgRNAs and the nickase? (1 tube with nickase D10A + sgRNA1 + sgRNA2).
What concentrations/volumes do you use to form the RNP complexes prior to addition to the electroporation cuvette.
How many ES cells do you electroporate with RNP complexes?
Thank you
Question
Do you know a scientific article indicating the adhesion time of murine primary myoblast cells? It must be around 3 to 5 hours?
Question
Hello everyone,
Is there a free ImageJ / Figi macro to quantify nuclear fusion indexes, myotube size, nucleus number in myotube ...? (From Myosine / Dapi image)
Thanks!

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Projects

Projects (3)
Project
GASP-2 and GASP-1 are two orthologous secreted proteins with several domains. They have a strong affinity with myostatin and GDF11, which are members of the TGFβ family and which are essential in the regulation of musculoskeletal development. In order to better understand the role of GASP-2 in musculoskeletal development and the molecular mechanisms involved, different approaches were developed during my thesis.