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Citations since 2017
10 Research Items
PhD Molecular and Cell Biology - PostDoctoral Researcher - Laboratory of Chronic and Neurologic Diseases Epidemiology - Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin. Focusing on Genetic Risk Factors for Breast Cancer in Benin. Contact : firstname.lastname@example.org
Background GASP-2 is a secreted multi-domain glycoprotein known as a specific inhibitor of myostatin and GDF-11. Here we investigate the role of GASP-2 on myogenesis and the effect of its glycosylation on its activity. Methods GASP-2 overexpression or knockdown by shRNAs were carried out on C2C12 myoblasts cells. In silico analysis of GASP-2 prote...
Background/aims: Myostatin is known as a powerful negative regulator of muscle growth playing a key role in skeletal muscle homeostasis. Recent studies revealed that myostatin-deficient mice lead to an increase of insulin sensitivity, a decrease of adiposity and a resistance to obesity, showing that myostatin can also impact on metabolism. Thus, m...
The transcriptional co-activator PGC-1α has been reported to play a key role in adaptive thermogenesis and to influence muscle homeostasis and growth in mouse and human. PGC-1α has a complex structure with multiple protein domains whose gene is controlled by two promoters and is subject to alternative splicing events. In cattle, very little is curr...
Myogenesis is a multistep process taking place during pre- and postnatal stages for muscle formation, growth, and regeneration. It is a highly regulated process involving many molecular factors which act during myoblast proliferation and differentiation. To provide new insights into the molecular mechanisms and interactions behind the regulation of...
19th PhD Student Seminar of the Genetic Animal Department - 2016, March 16 and 17 - Toulouse
PhexL222P mouse is a new ENU mouse model for XLH disease due to Leu to Pro amino acid modification at position 222. PhexL222P mouse is characterized by growth retardation, hypophosphatemia, hypocalcemia, reduced body bone length, and increased epiphyseal growth plate thickness and femur diameter despite the increase in PHEXL222P expression. Actuall...
Nous présentons une nouvelle stratégie instrumentale et computationnelle appelée FAMOUS (pour fast algorithm for three-dimensional (3D) multiphoton microscopy of biomedical structures) basée sur une approche de microscopie multiphotonique assistée par calcul. Le but est l’amélioration visuelle des images d'échantillons biologiques épais offrant ain...
We present a new instrumental and computational pipeline named FAMOUS: f ast a lgorithm for three-dimensional (3D) m ultiphoton microscopy of bi o medical str u cture s . This pipeline rests on a MPM strategy combined with an original 3D post-processing computational approach. In the present work, FAMOUS approach is devoted to the 3D imaging of the...
Muscle atrophy is associated with many diseases including genetic disorders, sarcopenia, or cachexia syndromes. Myostatin (Mstn), a transforming growth factor-beta (TGF-β) member, plays a key role in skeletal muscle homeostasis as a powerful negative regulator. Over the last decade, about 15 clinical trials aimed at inhibiting the Mstn pathway, fai...
While GASP‐1 and GASP‐2 proteins are known to regulate myogenesis by inhibiting myostatin, their structural organization suggests a putative role as multivalent protease inhibitors controlling different protease activities. In this study, we show the non‐competitive and competitive anti‐trypsin activities of the full‐length GASP‐1 and GASP‐2 protei...
GASP-1 and GASP-2 are two closely related proteins structurally characterized by several protease inhibitor modules and identified as inhibitors of several members of the TGF-ß family such as myostatin or GDF-11, negative regulators of myogenesis and osteogenesis, respectively. Despite the common structural organization of GASPs proteins, their dif...
Myostatin (Mstn) is a negative regulator of muscle growth and allows to control the balance between muscle hypertrophy and atrophy. As in many muscle diseases, this balance is altered. Knockout Mstn-/- mice exhibit a hypermuscular phenotype characterized by an hyperplasia and hypertrophy of muscle fibers and a decrease in fat mass. One of the lines...
We wish to electroporate ES cells with protein nickase D10A and two sgRNAs targeting our gene of interest.
Before adding the RNP complexes to the electroporation cuvette containing our cells :
Should the RNP complexes be formed independently? (1 tube with nickase D10A + sgRNA1 and 1 tube with nickase D10A + sgRNA-2) or mix the 2 sgRNAs and the nickase? (1 tube with nickase D10A + sgRNA1 + sgRNA2).
What concentrations/volumes do you use to form the RNP complexes prior to addition to the electroporation cuvette.
How many ES cells do you electroporate with RNP complexes?
Is there a free ImageJ / Figi macro to quantify nuclear fusion indexes, myotube size, nucleus number in myotube ...? (From Myosine / Dapi image)
GASP-2 and GASP-1 are two orthologous secreted proteins with several domains. They have a strong affinity with myostatin and GDF11, which are members of the TGFβ family and which are essential in the regulation of musculoskeletal development. In order to better understand the role of GASP-2 in musculoskeletal development and the molecular mechanisms involved, different approaches were developed during my thesis.