Alexey Olovnikov

Alexey Olovnikov
Russian Academy of Sciences | RAS · Institute of Biochemical Physics

28.92
 · 
PhD

About

70
Publications
6,881
Reads
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3,162
Citations
Research Experience
September 1997 - present
Russian Academy of Sciences
Position
  • Leading researcher
Description
  • theorist, PhD, olovnikov@gmail.com
January 1977 - September 2020
Russian Academy of Sciences
Position
  • Leading researcher in Institute of Biochemical Physics

Publications

Publications (70)
Article
During a cell's lifespan, DNA break formation is a common event, associated with many processes, from replication to apoptosis. Most of DNA breaks are readily repaired, but some are meant to persist in time, such as the chromosome ends, protected by telomeres. Besides them, eukaryotic genomes comprise shorter stretches of interstitial telomeric rep...
Article
Full-text available
It is supposed that the development and aging of multicellular animals and humans are controlled by a special form of the clock mechanism - a chronograph. The development of animals and their aging are interconnected by the program of the species lifespan that has been selected in the evolution of each species to fit the resources of its ecological...
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Full-text available
The non-coding and repetitive sequences constitute a great amount of higher eukaryotes genomes, but the elucidation of its role and mechanisms of action is now at the very beginning. Here we found, that internal telomeric repeats in Danio rerio are colocalized with some repetitive elements, namely, hAT and EnSpm repeats, which are highly represente...
Article
We found that some morphological properties of the pineal gland and submandibular salivary gland of mice are significantly distinct at the new and full moon. We suppose that the differences are initiated by the displacements of the electron-dense concretions in the secretory vesicles of pinealocytes. This presumably occurs under the influence of th...
Article
Noncoding and repetitive sequences make up a large part of the genome of high eukaryotes, but the elucidation of their roles and mechanisms of action are poorly understood. In this work, we found that interstitial telomeric repeats in the genome of Danio rerio colocalize with repetitive elements, including hAT and EnSpm, which are widely represente...
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Full-text available
An explanation of the role of primordial germ cell (PGC) migration during embryogenesis is proposed. According to the hypothesis, various PGCs during their migrations through an early embryo are contacting with anlagen of organs and acquiring nonidentical organ specificities. An individual PGC gets such an organ specificity, which corresponds to sp...
Article
Aging could be a consequence of the programmed partial dedifferentiation of brain neurons that is performed through nonrandom losses of chronomeres-short perichromosomal DNA molecules that also participate in maintenance of cellular differentiation state. Process of their sequential losses, implementing as the “relay-race partial dedifferentiations...
Article
An suggestion is put forward according to which the incomplete restoration of membranes in irradiated brain cells can self-perpetuate, down regulate their activity and accelerate ageing.
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Full-text available
A hypothetical mechanism for rapid and nonrandom emergence of evolutionary adaptations is proposed. It is supposed that some transcription factors and transcription regulators that are able to cross membranes can leave the cells of their origin and move within the organism using a specialized transport system when individual development occurs unde...
Article
SUMMARYA new virobacterial agglutination (VBA) test for the immunodiagnosis of plant viruses is described. The test is based on the agglutination of Staphylococcus aureus cells by virus particles after treatment of the cells with homologous antiserum. The agglutination occurs within 1–5 min. The sensitivity of the test is 0·1-0·4 μg virus/ml and is...
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Full-text available
As the basis for the lifelong clock and as a primary cause of aging, a process of shortening of hypothetical perichromosomal DNA structures termed chronomeres is proposed in the CNS. The lifelong clock is regulated by the shortening of chronomere DNA in postmitotic neurons of the hypothalamus. Shortening of these DNA sequences occurs in humans on a...
Article
As the basis for the lifelong clock and as a primary cause of aging, a process of shortening of hypothetical perichromosomal DNA structures termed chronomeres is proposed in the CNS. The lifelong clock is regulated by the shortening of chronomere DNA in postmitotic neurons of the hypothalamus. Shortening of these DNA sequences occurs in humans on a...
Article
Full-text available
The concept of paragenome is proposed, which is considered as a transient array of short DNA molecules appearing on the chromosome surface during development for the control of genome. The paragenome consists of printomeres, chronomeres, and phylomeres. Chronomeres and printomeres are obligatory for cells of certain differentiation lineages, but th...
Article
According to the redusome hypothesis, the aging of an organism is determined by the shortening of chronomeres (small perichromosomal linear DNA molecules). In this paper, a presumptive role for infradian hormonal rhythms is considered. Endogenous infradian rhythms are supposed to actively interact with those hormonal shifts which are governed by an...
Article
Full-text available
The redusome hypothesis of aging and the control of biological time in individual development is proposed. Redusomes are hypothetical perichromosomal particles arising in differentiation events during morphogenesis of an organism. The linear molecule of DNA covered with proteins in the redusome is assumed to be a copy of a segment of chromosomal DN...
Article
The redusome hypothesis of aging and biological age control (Olovnikov, Biochemistry (Moscow) 2003, vol. 68, pp. 2-33; http://protein.bio.msu.su/biokhimiya/contents/v68/ToC6801.htm.) is discussed. Though the main part of telomere-related predictions (Olovnikov, 1971, 1973) have successfully been confirmed (end under-replication of linear DNA molecu...
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Full-text available
It is hypothesized that gene function is modulated through the action of the ion channels of the internal nuclear membrane, and that this underlies the phenomenon of dominance and some epigenetic effects. The topographic specificity essential to gene regulation by injecting portions of ions--ion fountains--is ensured by special fountain RNAs (fRNAs...
Article
Full-text available
It is hypothesized that gene function is modulated through the action of the ion channels of the internal nuclear membrane, and that this underlies the phenomenon of dominance and some epigenetic effects. The topographic specificity essential to gene regulation by injecting portions of ions—ion fountains—is ensured by special fountain RNAs (fRNAs),...
Article
Full-text available
According to the proposed hypothesis, the memory of a cell about the achieved state of cytodifferentiation is based on the existence of a postulated genetic structure termed here as a "printomere". A printomere is a relatively small linear DNA fragment which is laterally located on the chromosomal body and armed at its termini with peculiar analogs...
Article
Full-text available
Nature Biotechnology journal featuring biotechnology articles and science research papers of commercial interest in pharmaceutical, medical, and environmental sciences.
Article
We propose a possible mechanism for the telomere shortening signal. The suggested solution of this as yet unsolved enigma-how cell senescence is causally linked to telomere shortening-is based on a 'fountain theory' of modulation of eukaryotic gene expression, in which gene expression is modulated by ionic channels of the inner nuclear membrane. Th...
Article
It is proposed that hypermutation of the Ig gene is based on the use of "misprimers" (MPs) capable of competing with a true primer for the DNA. The MP is a product of the cleavage of the nascent transcript. Each MP has an erroneous base on its 3; terminus. Wobbling of the unpaired 3; end of the MP forces the DNA polymerase to make an error.
Article
Full-text available
A fountain mechanism of quantitative regulation of gene expression level during development is proposed. The mechanism is based on postulated ability of a special class of RNA molecules, so called fountain RNAs (fRNAs), to induce passive and selective ionic channels in the internal nuclear membrane. Ions diffuse via channel from the nuclear lumen i...
Article
A molecular mechanism of reading of positional information by cells in morphogenesis and regeneration is proposed. It permits to translate the genome information into three-dimensional form of an organism. In the mechanism, a new fraction of DNA, so called "location DNA" is used which is suggested as a substitution instead of a former "egoistic" DN...
Article
Full-text available
In 1971 I published a theory in which I first formulated the DNA end replication problem and explained how it could be solved. The solution to this problem also provided an explanation for the Hayflick Limit, which underpins the discovery of in vitro and in vivo cell senescence. I proposed that the length of telomeric DNA, located at the ends of ch...
Article
A possible effect of incomplete terminal DNA repair providing for shortening of telomeric DNA in the somatic cells, specifically postmitotic neurons, has been discussed. The effect of incomplete terminal repair can and must show up independently from the effect of incomplete terminal DNA replication proposed earlier.
Article
Incomplete repair of the DNA double helix terminus is an independent mechanism of the telomeric DNA shortening in non-dividing cells. This effect of incomplete terminal DNA repair may initiate aging of postmitotic neuroendocrine cells or even whole postmitotic organisms, if loss of the buffer telomeric DNA (due to incomplete terminal repair) is fol...
Article
Earlier we proposed an idea about the direct proportional dependence between the length of buffer DNA at the ends of chromosomes, i.e., telomeric DNA, and the life span of organisms. In these publications, we predicted the phenomenon of shortening of telomeric DNA as a result of under-replication (incomplete replication) of terminal DNA sequences....
Article
A technique of antierythrocyte antibody detection is described. It as based on co-agglutination of red blood cells and S. aureus cells. The method is unsophisticated and non-laborious. It provides massive agglutination, its sensitivity is one order of magnitude higher than that of the Coombs test.
Article
Recently, great success has been attained in studies on agents responsible for food intoxication. The modern stage of research on food intoxication of bacterial etiology is qualitatively different from previous ones, since many investigators ignored the determination of food toxic agents and instituted direct investigation of biologically active su...
Article
An aggregate-haemagglutination test has been used for determining antierythrocyte autoantibodies. The first antiglobulin variant of the test allows us to establish the presence of antibodies in 33 cases with a negative direct Coombs' test. The test II (antiantiglobulin variant) proved to be positive in 88 cases of auto-immune haemolytic anaemia (AI...
Article
The possibility has been demonstrated of using the method of aggregate-haemagglutination for the detection of B. cereus exo-enterotoxin in both food products and culture media. It has been established that 0.004 mug/ml of enterotoxin can be detected by this method. The applied antisera to B. cereus enterotoxin did not yield cross reactions with ent...
Article
An aggregate-haemagglutination test has been used for determining antierythrocyte autoantibodies. The first antiglobulin variant of the test allows us to establish the presence of antibodies in 33 cases with a negative direct Coombs’ test. The test II (antiantiglobulin variant) proved to be positive in 88 cases of autoimmune haemolytic anaemia (AIH...
Article
A new test for detection of antierythrocyte antibodies is described; one of its variants is three orders of magnitude more sensitive than the classic Coombs test. The proposed procedures are based on the agglutination of the red blood cells following addition of test-erythrocytes coated with the aggregated proteins of an antiglobulin serum. Results...
Article
Various methodological approaches for detection of antibodies in autoimmune hemolytic anemia were considered. An aggregate hemagglutination test was used enabling the sensitivity of determination after Coombs to be increased 1000 times. In 114 of the patients with a negative direct Coombs test antierythrocytic antibodies were revealed with the aid...
Article
An aggregate-hemagglutination test for antierythrocytic antibodies is suggested. In one of its forms it is three times as sensitive as the indirect Coombs' test. The test is based on agglutination of the erythrocytes under investigation on treatment with test-erythrocytes coated with aggregated antiglobulin serum proteins.
Article
Full-text available
The possibility of using the aggregate-hemagglutination technique for detection of Bacillus cereus exo-enterotoxin in foodstuffs and culture media is shown. A 0.004-mug quantity of enterotoxin per ml can be detected by this method.
Article
A theory of marginotomy has been proposed to explain the limitation of the cell doubling potential of clones of normal somatic cells. Marginotomy of DNA is the shortening of the replica with respect to the template. Two possible mechanisms of marginotomy are discussed. The first mechanism is a DNA-polymerase, postulated to have a catalytically inac...
Article
A method of sensitizing sheep's erythrocytes with proteins based on the use of a stable tetrazonium salt is suggested. After fixation of protein to them by means of a covalent azo-bond, the erythrocytes can be used in the local passive hemolysis test for the detection of rabbit or mouse spleen cells secreting antibodies against protein antigens. A...
Article
A new method for determining antigen content, based on the agglutination of erythrocytes coated by polycondensed proteins of immune serum, is proposed. It was assumed that successful sensitization could be achieved only if the active centre of the antibody molecule was kept at some distance from the erythrocyte surface. This may be done by the intr...

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The goal is the same as for all researchers of this insidious process, to slow down its running.