Alexey Moskalev

Russian Scientific Center of Surgery named after Academician Petrovsky · Institute of longevity

The life span alteration after γ-irradiation and/or paraquat treatment in Drosophila in wild type strain Canton-S and strains with mutations of heat shock factor (1–4 alleles) and heat shock proteins (Hsp70Ba 304 , Hsp83 e6A , Hsp22 EY09909 , Hsp67Bb EY099099 ) was investigated. Chronic low-dose rate γ-irradiation (0.017 and 0.17 cGy/h) on pre-imag...

With the rapid expansion of aging biology research, the identification and evaluation of longevity interventions in humans have become key goals of this field. Biomarkers of aging are critically important tools in achieving these objectives over realistic time frames. However, the current lack of standards and consensus on the properties of a relia...

The recent unprecedented progress in ageing research and drug discovery brings together fundamental research and clinical applications to advance the goal of promoting healthy longevity in the human population. We, from the gathering at the Aging Research and Drug Discovery Meeting in 2023, summarised the latest developments in healthspan biotechno...

Biological age is a personalized measure of the health status of an organism, organ, or system, as opposed to simply accounting for chronological age. To date, there have been known attempts to create estimators of biological age based on various biomedical data. In this work, we focused on developing an approach for assessing heart biological age...

The phenomenon of compartmentalization is one of the key traits of life. Biological membranes and histohematic barriers protect the internal environment of the cell and organism from endogenous and exogenous impacts. It is known that the integrity of these barriers decreases with age due to the loss of homeostasis, including age-related gene expres...

The extracellular matrix (ECM) is a complex three-dimensional network of macromolecules that provides structural support for the cells and plays a significant role in tissue homeostasis and repair. Growing evidence indicates that dysregulation of ECM remodeling contributes to various pathological conditions in the body, including age-associated dis...

Introduction: Multiple interventions have demonstrated an increase in mouse lifespan. However, non-standardized controls, sex or strain-specific factors, and insufficient focus on targets, hinder the translation of these findings into clinical applications. Areas covered: We examined the effects of genetic and drug-based interventions on mice fr...

Significance: Currently, a large amount of evidence of beneficial effects of diets enriched with polyphenols on various aspects of health has been accumulated. These phytochemicals have a geroprotective potential slowing down the pathological processes associated with aging and ensuring longevity. In this study, a comprehensive analysis was conduc...

Worldwide the aging population continues to increase, so the concept of healthy longevity medicine has become increasingly significant in modern society. Berberis vulgaris L. fruits serve as a functional food supplement with a high concentration of bioactive compounds, which offer numerous health-promoting benefits. The goal of this study was to in...

Geroprotectors are substances that slow down aging process and can be used for prevention of age-related diseases. Geroprotectors can improve functioning of various organ systems and enhance their homeostatic capabilities. We have developed a system of criteria for geroprotectors and proposed their classification based on the mechanisms of their ac...

Epigenetic aging is a hot topic in the field of aging research. The present study estimated epigenetic age in long-lived individuals, who are currently actively being studied worldwide as an example of successful aging due to their longevity. We used Bekaert’s blood-based age prediction model to estimate the epigenetic age of 50 conditionally “heal...

In recent years, there has been a focus on breeding wheat with high anthocyanin levels in order to improve food quality and human health. The objective of this study was to examine the antioxidant and geroprotective properties of wheat bran extracts using both in vitro and in vivo research methods. Two wheat lines were used: one with uncolored peri...

Plant polyphenols are characterized by a wide range of biological activities, including antioxidant properties, and have a high geroprotective potential. The purpose of this work was to investigate the effect of the extract of rowan berries (Sorbus aucuparia L.) on the lifespan and stress resistance of Drosophila melanogaster with the identificatio...

Aging is accompanied by a widespread deterioration in the state of the body (physiological and psychological) with the concomitant manifestation of neurodegenerative diseases (dementia, Alzheimer’s disease, Parkinson’s disease). Biologically active substances contained in the fruits of chokeberry have previously shown a neuroprotective potential in...

The search for drugs that slow down the rate of aging and stimulate radioresistance is an urgent task of biology, ecology, and medicine. In this work we have studied the effects of trichostatin A and berberine on the lifespan, resistance to the paraquat prooxidant and acute gamma irradiation of the Drosophila melanogaster fruit fly. Trichostatin A...

Epigenetic mechanisms play a leading role in the regulation of gene expression and the coordination of biological processes, influencing the aging rate and the organism’s lifespan. An important role in the implementation of these mechanisms is played by small RNAs which suppress the activity of own targets through the RNA interference and provide t...

INTRODUCTION. Due to the demographic aging of the population, the problem of treatment of age-related diseases and prevention of premature aging in modern healthcare has become particularly urgent. One of the most promising approaches is the impact on the molecular mechanisms of aging, including the activation of adaptive systems and suppression of...

Worldwide aging population continues to increase, so the concept of healthy longevity medicine has become increasingly significant in modern society. Berberis vulgaris L. fruits serve as a functional food supplement with a high concentration of bioactive compounds, which offer numerous health-promoting benefits. The goal of this study was to invest...

Background. Biological age is a better predictor of morbidity and mortality associated with chronic age-related diseases than chronological age. The estimated difference between biological and chronological age can reveal an individual’s rate of aging.Aim. The aim of this study was to assess the association of cardiovascular risk factors with the r...

With the rapid expansion of aging biology research, the identification and evaluation of longevity interventions in humans have become key goals of this field. Biomarkers of aging are critically important tools in achieving these objectives over realistic time frames. However, the current lack of standards and consensus on the properties of a relia...

Geroprotectors are compounds that extend lifespan of model organisms. Currently more than 200 geroprotectors are known, however only few of them are ready to be tested in clinical trials so the discovery of new geroprotectors is an important problem. Here we propose a novel approach for geroprotector discovery based on machine learning and omics da...

The term neuronutrition has been proposed as part of nutritional neuroscience, studying the effects of various dietary components on behavior and cognition. Other researchers underline that neuronutrition includes the use of various nutrients and diets to prevent and treat neurological disorders. The aim of this narrative review was to explore the...

Torin-2, a synthetic compound, is a highly selective inhibitor of both TORC1 and TORC2 (target of rapamycin) complexes as an alternative to the well-known immunosuppressor, geroprotector, and potential anti-cancer natural compound rapamycin. Torin-2 is effective at hundreds of times lower concentrations and prevents some negative side effects of ra...

Гистонацетилтрансферазы семейства CBP/p300 участвуют в регуляции транскрипции и в осуществлении ряда биологических процессов (пролиферации и дифференцировки клеток, развитии организма, регуляции стресс-ответа и метаболизма). В исследовании на плодовой мушке Drosophila melanogaster нами впервые проанализировано влияние сверхэкспрессии и нокдауна ген...

The transcriptional coactivators Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are the main downstream effectors of the evolutionarily conserved Hippo signaling pathway. YAP/TAZ are implicated in the transcriptional regulation of target genes that are involved in a wide range of key biological processes a...

A prospective randomized comparative placebo-controlled double-blind study was carried out based on Arterial Indices model of biological age. The study involved 60 men and women aged 40–65 years that were randomly divided into two equal groups of 30 people: the main group and the control one. The study participants from the main group received a di...

The relationships between blood biomarkers, frailty, and the risk of death of people diagnosed with COVID-19 is unclear. In the current investigation we decided to analyze the collective effect of multiple bio-markers (laboratory markers of inflammation, blood biochemistry deviations, comorbidity, demographics) on mortality in people diagnosed with...

Background: Kivach Clinic has developed a special medical spa program to prevent aging-related conditions in metabolic, cardio-vascular, and neurological states. Spa programs modify diet, physical activity, and lymphatic drainage, as it deteriorates with aging. We investigated its influence on the blood markers of biological age of patients during...

Transposable elements (TEs) are an important part of eukaryotic genomes. The role of somatic transposition in aging, carcinogenesis, and other age-related diseases has been determined. This review discusses the fundamental properties of TEs and their complex interactions with cellular processes, which are crucial for understanding the diverse effec...

Hydrogen sulfide (H 2 S) is one of the most important gasotransmitters that affect lifespan and provide resistance to adverse environmental conditions. Here we investigated geroprotective effects of the individual and simultaneous overex-pression of genes encoding key enzymes of H 2 S biosynthesis-cystathionine-β-synthase (CBS) and cystathionine-γ-...

BACKGROUND : The problem of metabolic syndrome is considered a demographic catastrophe. According to WHO experts, «by 2025, the prevalence of metabolic syndrome (MS) in the world will amount to more than 300 million people, and in the next 25 years it is expected to increase by 50%.» The pathophysiological mechanisms of MS formation and the role of...

Extracellular matrix (ECM) is an extracellular tissue structure that, in addition to mechanical support to the cell, is involved in regulation of many cellular processes, including chemical transport, growth, migration, differentiation, and cell senescence. Age-related changes in the structure and composition of the matrix and increase of ECM stiff...

INTRODUCTION. The impact on a human organism of health resort detox programs based on therapeutic governance of lymphatic tissues drainage on human metabolism and a possibility to reduce intoxication and inflammation level has not been evaluated yet. AIM. To make the first attempt to objectively estimate the influence of health resort body detoxifi...

The transcriptional coactivators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are the main downstream effectors of the evolutionary conserved Hippo signaling pathway. YAP/TAZ is implicated in the transcriptional regulation of target genes that are involved in a wide range of key biological processes affe...

Differences in the size and shape variability of the female offspring wing obtained from irradiated parents of inbred and outbred groups of Canton-S strain of Drosophila melanogaster were estimated using geometric morphometry methods. The groups were kept under conditions of chronic irradiation at an exposure dose rate of 2,5 mGy/h. The absorbed do...

The search for drugs that affect the radioresistance of an organism is an urgent task of radiobiology and medicine. The effects of retinoic acid, enoxacin, KN-93, and UNC-0646 on resistance to γ-irradiation at doses of 120 and 800 Gy, as well as on the level of DNA damage and the efficiency of DNA repair were studied for the first time using the Dr...

Chokeberry contains a wide range of biologically active substances with potential geroprotective activity. An ethanolic extract of chokeberry ×Sorbaronia mitschurinii (ABE) has previously been found to have a positive effect on the lifespan of Drosophila melanogaster individuals in short-term exposure (two weeks). The present study evaluated the ef...

Aging is one of the global challenges of our time. The search for new anti-aging interventions is also an issue of great actuality. We report on the success of Drosophila melanogaster lifespan extension under the combined influence of dietary restriction, co-administration of berberine, fucoxanthin, and rapamycin, photodeprivation, and low-temperat...

Honeysuckle Lonicera pallasii (Lonicera caerulea L.) is an excellent source of anthocyanins which have a number of health-promoting properties mainly associated with antioxidant and anti-inflammatory activities. Cyanidin-3-O-glucoside (C3G) is one of the most common anthocyanins naturally found in honeysuckle. The goal of the present study was to i...

Aging biology is a promising and burgeoning research area that can yield dual-purpose pathways and protein targets that may impact multiple diseases, while retarding or possibly even reversing age-associated processes. One widely used approach to classify a multiplicity of mechanisms driving the aging process is the hallmarks of aging. In addition...

Plants hold all records in longevity. Their aging is a complex process. In the presented review, we analyzed published data on various aspects of plant aging with focus on any inferences that could shed a light on aging in animals and help to fight it in human. Plant aging can be caused by many factors, such as telomere depletion, genomic instabili...

The gasotransmitter hydrogen sulfide (H2S) is an important biological mediator, playing an essential role in many physiological and pathological processes. It is produced by transsulfuration − an evolutionarily highly conserved pathway for the metabolism of sulfur-containing amino acids methionine and cysteine. Cystathionine-β-synthase (CBS) and cy...

Old age is a crucial risk factor for severe coronavirus disease 2019 (COVID-19), with serious or fatal outcomes disproportionately affecting older adults compared with the rest of the population. We proposed that the physiological health status and biological age, beyond the chronological age itself, could be the driving trends affecting COVID-19 s...

Annual fish of the genus Nothobranchius are promising models for aging research. Nothobranchius reproduces typical aspects of vertebrate aging, including hallmarks of brain aging. Meclofenoxate (MF) is a well-known compound that can enhance cognitive performance. The drug is prescribed for asthenic conditions, trauma, and vascular diseases of the b...

Geroprotectors slow down aging and promote healthy longevity in model animals. Although hundreds of compounds have been shown to extend the life of laboratory model organisms, clinical studies on potential geroprotectors are exceedingly rare, especially in healthy elders. This review aims to classify potential geroprotectors based on the mechanisms...

Aging is the single largest risk factor for most chronic diseases, and thus possesses large socioeconomic interest to continuously aging societies. Consequently, the field of aging research is expanding alongside a growing focus from the industry and investors in aging research. This year's 8th Annual Aging Research and Drug Discovery (ARDD) meetin...

Maintenance of active longevity, preservation of physical activity, and prevention of decreased mobility associated with injury or age of patients are among the most urgent tasks for modern healthcare. The suppression of pathological processes and activation of defense systems at the cellular and organismal levels are the main routes for solving th...

The COVID-19 pandemic enforced societies to rapidly restructure industrial, social security, and public health care processes. Several countries, where organized anti-epidemic measures were implemented, indicated the efficacy of strict adherence to measures that flexibly balance between self-isolation and social distancing. The population group at...

From the biomedicine point of, view ageing is a natural process, characterized by a gradual decrease in the physiological integrity and adaptive abilities of the body, leading to a violation of its functions and an increase in the risk of death with age. Demographic aging of the population is a serious socio-economic problem, both in Russia and aro...

The rhythmic functioning of the molecular circadian clock in various tissues and nutrition has a significant impact on the duration and quality of life, as well as on the rate of aging of the body. It is known that body weight correlates with the expression of various genes and the risk of death at different ages. In this study, an attempt was made...

The increase in life expectancy, leading to a rise in the proportion of older people, is accompanied by a prevalence of age-related disorders among the world population, the fight against which today is one of the leading biomedical challenges. Exploring the biological insights concerning the lifespan is one of the ways to provide a background for...

В настоящей работе проведено исследование сочетанных эффектов пан-нейрональной конститутивной экспрессии гена cry и применения хронобиотика KL001 в геропротекторной концентрации 5 мкМоль/мл на модели Drosophila melanogaster. Гипотеза: KL001 стабилизирует криптохром и увеличивает горметиче-ский эффект его сверхэкспрессии. Экспериментально установлен...

The infusion of coronavirus disease 2019 (COVID-19) patients with mesenchymal stem cells (MSCs) potentially improves clinical symptoms, but the underlying mechanism remains unclear. We conducted a randomized, single-blind, placebo-controlled (29 patients/group) phase II clinical trial to validate previous findings and explore the potential mechanis...

BACKGROUND: ×Sorbaronia mitschurinii (also known as Aronia mitschurinii) is an intergeneric hybrid of×Sorbaronia fallax and Aronia melanocarpa. ×S. mitschurinii berry is a rich source of phytochemicals such as flavonoids and anthocyanins, which have a broad range of health benefits and a great geroprotective potential. OBJECTIVES: The goal of the p...

Nutrition (diet composition and eating regimen) is one of the key factors of longevity. A balanced diet allows you to avoid essential nutrient deficiencies and hazardous substances to metabolic health. A healthy diet, containing enough dietary fibers, polyphenols, and metabiotics, maintains an optimal quantitative and qualitative balance of the mic...

The main aim of the current Research Topic was to contribute to establishing the methodological basis for developing, and regulatory adoption of, diagnostic criteria for aging and aging-related multimorbidity. The articles published in this research topic provided a broad and diverse exploration of clinical criteria for aging and old-age multimorbi...

Background: Abisil is an extract of Siberian fir terpenes with antimicrobial and wound healing activities. Previous studies revealed that Abisil has geroprotective, anti-tumorigenic, and anti-angiogenic effects. Abisil decreased the expression of cyclin D1, E1, A2, and increased the phosphorylation rate of AMPK. Objective: In the present study,...

Citation: Solovev, I.A.; Shaposhnikov, M.V.; Moskalev, A.A. Chronobiotics KL001 and KS15 Extend Lifespan and Modify Circadian Rhythms of Drosophila melanogaster. Clocks&Sleep 2021, 3, 429-441. https://doi. Abstract: Chronobiotics are a group of drugs, which are utilized to modify circadian rhythms targeting clock-associated molecular mechanisms. Th...

The roots of Vicatia thibetica de Boiss are a kind of Chinese herb with homology of medicine and food. This is the first report showing the property of the extract of Vicatia thibetica de Boiss roots (HLB01) to extend the lifespan as well as promote the healthy parameters in Caenorhabditis elegans (C. elegans). For doxorubicin- (Doxo-) induced prem...

Sulfonylureas are widely used oral anti-diabetic drugs. However, its long-term usage effects on patients’ lifespan remain controversial, with no reports of influence on animal longevity. Hence, the anti-aging effects of chlorpropamide along with glimepiride, glibenclamide, and tolbutamide were studied with special emphasis on the interaction of chl...

Chronobiotics is a group of drugs utilized to modify circadian rhythms targeting clock-associated molecular mechanisms. The circadian clock is known as a controller of numerous processes standing behind aging. Hypothesis: KL001 and KS15 targeting CRY, affect lifespan, locomotor activity and circadian rhythm of Drosophila melanogaster. We observed a...

Background Black chokeberry (Aronia melanocarpa (Micht.) Elliot) is a great dietary source of a wide range of biologically active compounds, such as anthocyanins, flavonoids, other phenolic compounds, vitamins, and minerals that have a wide range of health benefits. Scope and approach Here we provide an overview of the in vitro and in vivo effects...