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Alexey Yu Gorbachev

Alexey Yu Gorbachev
Zenome.io · Genomics

PhD

About

30
Publications
6,913
Reads
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446
Citations
Introduction
Alexey Yu Gorbachev currently works at the Genomics, Zenome.io. Alexey does research in Molecular Biology, Microbiology and Analytical Chemistry. Their current project is 'Zenome.io'.
Additional affiliations
September 2009 - October 2014
Research Institute for Physico-Chemical Medicine
Position
  • Researcher
Education
October 2009 - October 2014
Research Institute for Physico-Chemical Medicine
Field of study
  • PhD in Molecular biology and Biochemistry
September 2004 - July 2009
Lomonosov Moscow State University
Field of study
  • Specialist in Molecular Biology

Publications

Publications (30)
Article
Full-text available
The CRX gene encoding the cone-rod homeobox protein is a specific photoreceptor transcription factor crucial for retinal function. Persons temporarily residing in the Arctic zone during the polar summer may suffer from disturbances associated with extremely high ambient illumination. These environmental changes are mediated by retinal photoreceptor...
Article
Full-text available
The MGISEQ-2000 developed by MGI Tech Co. Ltd. (a subsidiary of the BGI Group) is a new competitor of such next-generation sequencing platforms as NovaSeq and HiSeq (Illumina). Its sequencing principle is based on the DNB and the cPAS technologies, which were also used in the previous version of the BGISEQ-500 device. However, the reagents for MGIS...
Article
Full-text available
Studies of suicide genetics can be divided into 3 periods: 1) period of classical methods of behavioral genetics; 2) candidate genes association studies, and 3) genome-wide association studies. The majority of studies using candidate genes strategy has been dedicated to main neurotransmitter systems (serotonine, catecholamines, GABA, excitatory ami...
Article
Differential expression of 30,003 genes was studied in the liver of female Wistar rats fed with isocaloric diets with the excess of fat, fructose, or cholesterol, or their combinations for 62 days using the method of whole-transcriptome pro-filing on a microchip. Relative mRNA expression levels of the Asah2, Crot, Crtc2, Fmo3, GSTA2, LOC1009122026,...
Article
Full-text available
C57black/6J female mice were fed an isocaloric diet with excess fat, fructose, cholesterol, or combinations of them for 62 days. In their liver, the differential expression of 30 003 genes was studied by full-chip transcriptional profiling on an Agilent One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling, version 6.8, a...
Preprint
Full-text available
Background MGISEQ-2000 developed by MGI Tech Co. Ltd. (a subsidiary of the BGI Group) is a new competitor of such next-generation sequencing platforms as NovaSeq and HiSeq (Illumina). Its sequencing principle is based on the DNB and cPAS technologies, which were also used in the previous version of the BGISEQ-500 device. However, the reagents for M...
Article
Full-text available
Aims: To study changes in the expression of genes in liver tissue of rats with impaired receptivity of Lep under the influence of high-fat and high-carbohydrate diet (HFCR) or / and Q supplementation. Materials and methods: 4 groups of six male Zucker-LEPRfa male rats were used in experiment. Within 61 days the animals of the 1st group (control) re...
Thesis
Full-text available
Genomics is a technology spreading to many areas of human activity. Today, it is widely used in medicine, biotechnology, agriculture, and the food industry. Genetic data grows exponentially, which can make genomics the primary generator of data on the Internet (more than Twitter and YouTube). The rapid development of genomics creates several proble...
Article
Full-text available
Despite the fact the term “proteome” was proposed to characterize a set of proteins in one of mycoplasma species, proteome response to various exposures in this bacteria are still obscure. Commonly, authors studying proteomic response on perturbation models in mycoplasmas use single approach and do not confirm their findings by alternative methods....
Article
Full-text available
Gene-centric approach was applied for a large-scale study of expression products of a single chromosome. Transcriptome profiling of liver tissue and HepG2 cell line was independently performed using two RNA-Seq platforms (SOLiD and Illumina) and also by Droplet Digital PCR (ddPCR) and quantitative RT-PCR. Proteome profiling was performed using shot...
Article
Full-text available
Protein degradation is a basic cell process that operates in general protein turnover or to produce bioactive peptides. However, very little is known about the qualitative and quantitative composition of a plant cell peptidome, the actual result of this degradation. In this study we comprehensively analyzed a plant cell peptidome and systematically...
Article
Full-text available
Human hepatoma HepG2 cells are used as an in vitro model of the human liver. High-throughput transcriptomic sequencing is an advanced approach for assessing the functional state of a tissue or cell type. However, the influence of experimental factors, such as the sample preparation method and inter-laboratory variation, on the transcriptomic profil...
Article
Full-text available
The avian bacterial pathogen Mycoplasma gallisepticum is a good model for systems studies due to small genome and simplicity of regulatory pathways. In this study, we used RNA-Seq and MS-based proteomics to accurately map coding sequences, transcription start sites (TSSs) and transcript 3′-ends (T3Es). We used obtained data to investigate roles of...
Article
Full-text available
Ovarian cancer ascites is a native medium for cancer cells that allows investigation of their secretome in natural environment. This medium is of interest as a promising source of potential biomarkers and also as a medium for cell-cell communication. The aim of this study was to elucidate specific features of malignant ascites metabolome and proteo...
Preprint
The avian bacterial pathogen Mycoplasma gallisepticum is a good model for transcriptional regulation studies due to its small genome and relative simplicity. In this study, we used RNA-Seq experiments combined with MS-based proteomics to accurately map coding sequences (CDSs), transcription start sites (TSSs) and transcription terminators (TTs) and...
Article
Full-text available
We present a systematic study of three bacterial species that belong to the class Mollicutes, the smallest and simplest bacteria, Spiroplasma melliferum, Mycoplasma gallisepticum, and Acholeplasma laidlawii. To understand the difference in the basic principles of metabolism regulation and adaptation to environmental conditions in the three species,...
Article
Full-text available
DNA repair is essential for the maintenance of genome stability in all living beings. Genome size as well as the repertoire and abundance of DNA repair components may vary among prokaryotic species. The bacteria of the Mollicutes class feature a small genome size, absence of a cell wall, and a parasitic lifestyle. A small number of genes make Molli...
Article
Mycoplasmas are the smallest known microorganisms, with drastically reduced genome sizes. One of the essential biochemical pathways lost in mycoplasmas is methylation-mediated DNA repair (MMR), which is responsible for correction of base substitutions, insertions, and deletions in both bacteria and higher organisms. We found that the histone-like p...

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