Alexey Chemeris

Alexey Chemeris
Ufa Scientific Center of the Russian Academy of Science | USC RAS · Institue of Biochemistry and Genetics

PhD

About

218
Publications
40,693
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855
Citations
Citations since 2016
118 Research Items
606 Citations
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140

Publications

Publications (218)
Article
Taraxacum hybernum Steven (Asteraceae family) native to Southeast Europe is a promising rubber-bearing dandelion species. In the 1930s, several phenotypically distinct forms were described in this dandelion, such as brown-achened and pinkish-achened. More than 70 years after termination of research of temperate zone rubber-bearing plants we were ab...
Article
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In this work, we propose a new method for identifying organisms of multiplex polymerase chain reaction (PCR) with arbitrary primers in silico (multiplex in silico RAPD-analysis) for the unique identification of living organisms. The results of computer modeling search of possible primer annealing sites in genomic DNA, and their convertation into th...
Article
COVID-19 pandemic highlighted the demand for the fast and reliable detection of viral RNA. Although various methods for RNA amplification and detection have been proposed, some limitations, including those caused by reverse transcription (RT), need to be overcome. Here, we report on the direct detection of specific RNA by conventional polymerase ch...
Article
In 2019, at the World Economic Forum, DNA data storage was indicated as one of the breakthroughs expected to radically impact the global socio-economic order. Indeed, dry DNA is a relatively stable substance and an extremely capacious information carrier. One gram of DNA can hold up to 455 exabytes, provided that one nucleotide encodes two bits of...
Article
Polymerase chain reaction (PCR) is the most widely used method for nucleic acids amplification. To date, a huge number of versatile PCR techniques have been developed. One of the relevant goals is to shorten PCR duration, which can be achieved in several ways. Here, we report on the results regarding nucleic acids amplification by convective PCR (c...
Article
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Successful domestication and introduction into culture of new forms of rubber-bearing dandelions require not only determination of the content and quality of rubber in them, but also accurate species identification and genetic certification of valuable lines and forms. The objects of our study were three samples of Taraxacum kok-saghyz Rodin KAZ08-...
Article
The objective of the study was to pre-evaluate the applicability of gender-specific nucleotide sequences in human neuroligin genes as alternative DNA markers of sex. A new polymorphic locus based on NLGNX and NLGNY genes was proposed to establish the sex attribute of human biomaterials. The significant difference in the location of these loci relat...
Article
As confirmation of the huge diversity of DNA content in the nuclei of plant cells, the boundary values of minimum and maximum parameters such as the number of chromosomes, varying in plants per haploid genome from two chromosomes to 480 (or even up to 720) chromosomes, as well as the "weight" sizes of genomes in picograms in the form of 1C-value wi...
Article
Although this article is more like scientific fantastic, the creation of “talking” dogs through genetic engineering cannot be ruled out in the near future. The transcription factor FOXP2, which is responsible for communication abilities and articulate speech in humans, may become a key target. This protein is very conservative and differs in differ...
Article
Single nucleotide substitutions (SNPs) account for the largest share of DNA polymorphism in virtually any organism, including humans, and have a significant impact on their life status. Detection of already known polymorphic nucleotides (SNP typing) is of great importance, which explains the extremely wide variety of existing methods for SNP analys...
Article
Polymerase chain reaction (PCR) is the main and widely used method for nucleic acids amplification. PCR, which developed almost 40 years ago, has undergone significant development. Different variations and significant modifications of the method were also proposed. However, despite the achievements, the improvement of PCR, due to the need to solve...
Article
To detect DNA polymorphism in dogs and their wild relatives, a fairly simple method was used in the form of virtual amplification of certain random areas of their genomes using multiplex RAPD analysis with primers with arbitrary nucleotide sequences, which does not require prior knowledge of the sequences of nitrogenous bases of the detected DNA fr...
Article
Neanderthals lived on the territory of present-day Western Europe for about 300 thousand years before the arrival of people of modern anatomical type, also called Cro-Magnons, about 50 thousand years ago. After that Neanderthal soon disappeared and what happened to them occupies the minds of many generations of paleoanthropologists, and over the ye...
Article
The reasons for the mysterious disappearance of Neanderthals about 40 thousand years ago, which occurred shortly after the appearance on their territory of people of the modern anatomical type - Cro-Magnons, occupy the minds of many generations of paleoanthropologists. The extinction of the Neanderthals lasted for 2-5 thousand years, depending on t...
Article
Detection and quantification of biotargets are important analytical tasks, which are solved using a wide range of various methods. In recent years, methods based on the isothermal amplification of nucleic acids (NAs) have been extensively developed. Among them, a special place is occupied by rolling circle amplification (RCA), which is used not onl...
Article
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The emergence of the new SARS-CoV-2 coronavirus has given rise to many enigmas, to which there are no answers yet. However, the degree of threat to humanity, due to the fact that by the beginning of February 2021, more than 100 million people were ill in the world, of which 2 million died, led to the fact that the efforts of many researchers were a...
Article
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In forensic medicine, it is necessary to establish the sexual identity of the owner of the analyzed biological material. To do this, it is necessary to use PCR to detect specific DNA sequences that are characteristic only of the Y chromosome. For these purposes, a number of loci are used, located on both the Y and X chromosomes but carrying certain...
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When writing this botanical and historical review, it was necessary to get acquainted with the works of different authors from the XVII to the XXI century, written in Latin, old Spanish, old French, Spanish, French, German, Dutch, English and Russian. It shows the development of knowledge about growing on the American continents rubber-bearing tree...
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Over the two decades that have passed since the development of loop amplification (Loop AMPlification, LAMP), that carry out to detect specific nucleic acid under isothermal conditions, it has undergone quite a lot of improvements. This review presents data represented methodological bases of about a hundred variations of the LAMP, classified accor...
Article
A rather simple method of detecting DNA polymorphism in dogs in the form of amplification of random fragments of the genome using RAPD analysis with primers with arbitrary nucleotide sequences, which does not require prior knowledge of the sequences of nitrogenous bases of the detected DNA fragments, is considered. However, information about the co...
Article
The possible scenarios of the transformation of ancient now extinct wolves into a proto-dogs and the domestication of the latter, which could occur repeatedly, often ending for various reasons, are considered. It is noted that only a few ancient dog breeds are similar to their wolf ancestors and the Siberian Husky stands out among them, especially...
Article
Information is given on the size and organization of the mitochondrial and nuclear genomes of dogs, including information on polymorphisms of some loci used to clarify the phylogenetic relationships of wolves and the first dogs, including hypotheses about the places of domestication of ancient now extinct wolves and the dates of these events. It is...
Article
The application of mini- and microsatellite polymorphisms of dog DNA, also referred to as VNTR- and STR-loci, respectively, in dog breeding and criminalistics is considered. Their use in dog breeding is shown to clarify pedigrees, establish paternity and purebred, as well as to differentiate breeds mainly in the form of microsatellite DNA polymorph...
Article
The structural organization of the mitochondrial genome of dogs is briefly considered, paying more attention to its variable areas, including the control region and its hypervariable parts HV1 and HV2, as well as a block of tandem decameric repeats and a homopolymer tract of cytosines and thymines. It is noted that the detected polymorphism of mito...
Article
Underground water sources and their movement in various rocks, including karsts, are of considerable interest for many reasons, among which human economic activity plays a significant role. Various compounds are used to trace underground waters – stable and radioactive isotopes, microorganisms and bacteriophages, solid particles as lycopodium spore...
Conference Paper
Tobacco hairy roots with constitutive expression of the NtEXPA5 expansin gene were obtained and analyzed, which were characterized by higher growth rates and increased productivity under normal conditions, as well as the action of stress factors.
Conference Paper
Mutagenesis of Triticum sinskajae was performed using sodium azide. The optimal concentration was 0.1 mM. Changes were observed in the length of the spike and stem in the experimental plants as compared with the control ones.
Article
Full-text available
Kok-saghyz (Taraxacum kok-saghyz Rodin) is an alternative to Hevea brasiliensis source of high quality natural rubber that is able to grow in temperate climates. However, the field cultivation of kok-saghyz is associated with a number of problems, such as the need for seed stratification, seedlings that are very sensitive to stress factors, laborio...
Article
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The year 2020 marks the 100th anniversary of the term "genome", proposed by the German botanist H.Winkler when describing parthenogenesis in the plant and animal kingdoms. The etymology of this word is unclear, but it seems to us that Winkler used a play on words, combining the morphemes "gene", "soma" and getting "genosom"(German). Then he decided...
Article
Full-text available
Identification of individuals has become an urgent problem for mankind. In the last three decades, STR-based DNA identification has actively evolved along with traditional biometric methods. Nonetheless, single-nucleotide polymorphisms (SNPs) are now of great interest and a number of relevant SNP panels have been proposed for DNA identification. He...
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The culture of hairy roots is a promising system for the development of various compounds of both primary and secondary metabolism. For the industrial cultivation of hairy roots are being developed various types of bioreactors, but its most effective design for these purposes has not yet been determined. We have designed and tested two versions of...
Article
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Taraxacum brevicorniculatum and Taraxacum kok-saghyz are promising rubber-bearing dandelion species. T. brevicorniculatum, in contrast to kok-saghyz, is characterized by high parameters of vegetative growth, high seed productivity, but it accumulates very little natural rubber in the roots. The aim of the study was to carry out of chemical mutagene...
Article
Polymerase chain reaction is the most commonly used approach for nucleic acids amplification. Despite the variety of PCR methods have been proposed, new techniques are being developed to improve this reaction. We found that, in general, mono- and disaccharides can serve as effective PCR enhancers. Unlike oligo- and polysaccharides, low molecular-we...
Article
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This review focuses primarily on betacoronavirus SARS-CoV-2, which caused a pandemic in early 2020 in the form of a dangerous highly contagious coronavirus infection COVID-19, considered together with the closely related viruses SARS-CoV and MERS-CoV. The taxonomy of betacoronaviruses and their genomic organization is briefly presented, including i...
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The absence of a logic comma in the title of this work "To prohibit genetically modified organisms (GMOs) is impossible to resolve!", which is similar to the classic phrase ―To execute impossible to pardon‖ is no coincidence, because we would like readers themselves to put it in the only correct place after reading the entire article. Therefore, we...
Article
Background An analysis of the spatial distribution of some taxonomically and ecologically related legumes in the Ural showed a nontrivial spatial distribution of related species of the genus Oxytropis DC of the Orobia Bunge section within the Uchalinsky uplands. Despite the similarities in ecology, these species practically do not grow together. Ex...
Article
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Root rubber-bearing plants are represented mainly by plants of the Asteraceae family, and the most valuable of them is the Taraxacum kok-saghyz. In the 21st century, worldwide interest in both science and production towards this group of plants is increasing, some of which, unlike Hevea, can be cultivated in the vast areas of the northern hemispher...
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A series of 5-oxo-4H-pyrrolo[3,2-b]pyridine derivatives was identified as novel class of highly potent antibacterial agents during an extensive large-scale high-throughput screening (HTS) program utilizing a unique double-reporter system—pDualrep2. The construction of the reporter system allows us to perform visual inspection of the underlying mech...
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DNA is undoubtedly the most important biological macromolecule and its initial discovery in the form of a phosphorus-rich compound made by the young Swiss biochemist F.Miescher 150 years ago in nuclei of white blood cells of pus and called nuclein, as well as the discovery of its spatial structure in the form of a double helix by the efforts of J.W...
Article
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Specific amplification of nucleic acids is a convenient and quick alternative to the culture-based method of detecting bacterial cells. However, conventional PCR and other amplification reactions can not differentiate between live bacteria and dead or dormant ones, and are also capable of amplifying DNA that persists for a long time and in a cell-f...
Article
CRISPR/Cas technology of genome editing is a powerful tool for making targeted changes in the DNA of various organisms, including plants. The choice of the precise nucleotide sequence (protospacer) in the gene to be edited is important in the design of guide RNA, which can be carried out by specialized software. We review and compare all the known...
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The paper considers the problematic aspects of detecting a new coronavirus infection using RT-PCR, which often lead to false negative diagnostic results, which occur both at the preanalytic stage and during nucleic acid amplification, including the interpretation of the obtained data. The viral load in humans was evaluated and assumptions were made...
Article
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The nucleotide sequence of the chloroplast genome of the diploid wheat Triticum sinskajae A. Filat. et Kurk. was determined. The comparison of the assembled genome with the chloroplast genomes of some di- and polyploid species of the wheat-aegilops alliance revealed a high evolutionary conservativeness and proximity to the plastid genome of T. mono...
Article
The simplest and most common method of long-term storage of DNA samples at present is the storage of their frozen solutions, which, however, has a number of disadvantages, including the destruction of DNA molecules during freezing and thawing, as well as energy consumption and the likelihood of losing valuable samples in the event of possible accid...
Article
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CRISPR/Cas genome editing of plants is realized in three basic variants, including knockout mutations as indels, insertion of alien DNA fragments, and base editing via deamination of nitrogenous bases. The most important stages of the CRISPR/Cas-based genome editing are the choice of a target site, design of guide RNAs, creation of genetically engi...
Article
Full-text available
CRISPR/Cas genome editing of plants is realized in three basic variants, including knockout mutations as indels, insertion of alien DNA fragments, and base editing via deamination of nitrogenous bases. The most important stages of the CRISPR/Cas-based genome editing are the choice of a target site, design of guide RNAs, creation of genetically engi...
Article
Full-text available
[Article in Russian] Since the beginning of the 21st century, there has been a new surge of interest in alternative to rubber tree (Hevea brasiliensis (Willd. Ex A. Juss.) Mull. Arg.) sources of natural rubber. For the temperate zone, three species from the Asteraceae family were recognized as the most promising rubber-bearing plants: Taraxacum kok...
Article
Full-text available
Many pharmaceutical companies are avoiding the development of novel antibacterials due to a range of rational reasons and the high risk of failure. However, there is an urgent need for novel antibiotics especially against resistant bacterial strains. Available in silico models suffer from many drawbacks and, therefore, are not applicable for scorin...
Article
Introduction A variety of organic compounds has been reported to have antibacterial activity. However, antimicrobial resistance is the one of the main problems of current anti-infective therapy, and the development of novel antibacterials is one of the main challenges of current drug Methods Using our previously developed dual-reporter high-throug...
Article
Full-text available
Polymerase chain reaction (PCR) is one of the most common experimental methods for solving DNA analysis problems. The possibility of PCR experiment conduction and its success are vastly depend on oligonucleotide structures. Oligonucleotide primers are important component of any PCR, and therefore, there are a number of requirements for their design...
Presentation
Full-text available
[In Russian] Natural rubber today is an indispensable raw material in the manufacture of many industrial products, but above all, for the tire industry, where its share in the mixture with synthetic rubber for a car tire is up to 50%, for trucks up to 85%, and aviation tires by 100% made from natural rubber. To date, the main source of natural rubb...
Conference Paper
Full-text available
IX Всероссийская конференция с международным участием «Каучук и Резина-2019: традиции и новации» Современные подходы для получения натурального каучука из растений умеренного пояса Кулуев Булат Разяпович, зав. лаб., д.б.н., Князев Алексей Викторович, с.н.с., к.б.н., Мулдашев Альберт Акрамович, с.н.с., к.б.н., Чемерис Алексей Викторович, г.н.с., д.б...
Article
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Great relevance is the possibility of obtaining natural rubber from plant material grown in temperate-zone countries. Industrial cultivation of rubber-bearing plants under the conditions of Russia can be quite profitable, provided that the same plant material is used to produce several economically valuable metabolites. The aim of our work was to s...
Article
Aim and Objective Antibiotic resistance is a serious constraint to development of the new effective antibacterials. Therefore, the discovery of the new antibacterials remains one of the main challenges in modern medicinal chemistry. This study was undertaken to identify novel molecules with antibacterial activity. Materials and Methods Using our u...
Article
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The efficiency of Polymerase Chain Reaction (PCR) depends on multiple factors. The primers are very important components of PCR which determine the specificity of amplification. In this review, considerable attention is paid to the principles of selection of primers and the requirements for their design with the help of appropriate software. The pr...
Article
Full-text available
The ongoing research on CRISPR/Cas loci is so diverse, that can be called no fewer than “The CRISPR/Cas World” or even “The CRISPR/Cas Universe”. This review covers only a small part of existing data, with an emphasis on plant organisms. There are briefly described new methods for different stages of genome editing and for its various options such...
Article
The genetic diversity and phylogeny of root nodule bacteria, microsymbionts of plants of the genus Lupinaster Adans. (L. albus Link and L. pentaphyllus Moench), were studied, and also their symbiotic genes were analyzed. The bacterial strains studied were shown to be phylogenetically different; however, all of them are related to the Mezorhizobium...
Article
We generated transgenic tobacco plants (Nicotiana tabacum L.) with overexpression of the Arabidopsis thaliana CLAVATA3 (CLV3) gene which is known to be a negative regulator of cell division. Surprisingly, most of the 35S::CLV3 transgenic plants showed no phenotypic differences with the wild type plants. However, there were considerable changes in t...
Article
Full-text available
Natural rubber for a number of properties superior to synthetic rubber, so it remains to this day an indispensable natural raw material. In view of the vulnerability of the main source of rubber Hevea brasiliensis, it is urgent to search for alternative sources of this raw material. By the beginning of the XIX century, apart from H. brasiliensis, s...
Presentation
Full-text available
In 2018, kok-saghyz (Taraxacum kok-saghyz Rodin) and krym-saghyz (Taraxacum hybernum Steven) grew up on an experimental plot in an open field in the conditions of the Republic of Bashkortostan. The content of rubber in the roots of kok-saghyz reached in some plants up to 14% by dry weight. In the roots of the krym-saghyz, the rubber content reached...
Article
Full-text available
Natural rubber for a number of properties superior to synthetic rubber, so it remains to this day an indispensable natural raw material. In view of the vulnerability of the main source of rubber Hevea brasiliensis, it is urgent to search for alternative sources of this raw material. By the beginning of the XIX century, apart from H. brasiliensis, s...
Article
Full-text available
The purpose of the present study was to develop effective methods for callus induction, shoot regeneration, and rooting for Parasponia andersonii. Leaf explants of P. andersonii were placed on Lloyd and McCown’s (WPM) medium supplemented with various concentrations of TDZ and NAA for callus induction. Callus induction was observed on media containi...