Alexandre Ho-Pun-Cheung

Alexandre Ho-Pun-Cheung
Institut de Recherche en Cancerologie de Montpellier · URT

Ph.D.

About

35
Publications
1,891
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346
Citations

Publications

Publications (35)
Article
Full-text available
The alteration of mismatch repair (MMR) genes leads to microsatellite instability and plays a key role in colorectal cancer (CRC) pathogenesis and prognosis. The transcription factor NRIP1 is involved in intestinal tumorigenesis and is a good prognostic marker in CRC. In this study, we demonstrate that NRIP1 induces MSH2 and MSH6 MMR gene transcrip...
Article
In a previous phase II study (THERAPY), cetuximab and trastuzumab combination, as second‐line after progression with gemcitabine, showed disease stabilization in 27% of 33 patients with pancreatic carcinoma. In the present phase II multicenter study, we assessed the efficacy and tolerance of gemcitabine, trastuzumab plus erlotinib as first‐line tre...
Article
Background No treatment option was available for RAS-mutated (RASmt) metastatic colorectal cancer (mCRC) patients who progress after standard combined chemotherapies at the time of the study. After promising results in phase II, the aim of the present NEXIRI-2/PRODIGE 27 trial was to assess the 2-month non-progression rate for sorafenib (NEX) plus...
Article
Full-text available
The aberrant hypermethylation of BRCA1 promoter CpG islands induces the decreased expression of BRCA1 (Breast Cancer 1) protein. It can be detected in sporadic breast cancer without BRCA1 pathogenic variants, particularly in triple-negative breast cancers (TNBC). We investigated BRCA1 hypermethylation status (by methylation-specific polymerase chai...
Article
Full-text available
Triple-negative breast cancer (TNBC) has a worse prognosis compared with other breast cancer subtypes, and biomarkers to identify patients at high risk of recurrence are needed. Here, we investigated the expression of human epidermal receptor (HER) family members in TNBC and evaluated their potential as biomarkers of recurrence. We developed Time R...
Article
Full-text available
The TP53 gene plays essential roles in cancer. Conventionally, wild type (WT) p53 is thought to prevent cancer development and metastasis formation, while mutant p53 has transforming abilities. However, clinical studies failed to establish p53 mutation status as an unequivocal predictive or prognostic factor of cancer progression. The recent discov...
Data
Schematic illustration of the role of delta133p53ß in regulating RhoB pro-apoptotic activity. When delta133p53ß is expressed in cancer cells, RhoB is sequestered in the nucleus and its activity is inhibited. In the absence of delta133p53ß, RhoB can trigger its pro-apoptotic activities in the cytoplasm. (JPG)
Data
Sequences of primers and probes used for TaqMan real-time PCR assays. (DOCX)
Data
Analysis of RhoB-delta133p53ß interaction and of RhoC activity in CRC cell lines and RhoB localization in delta133p53α-overexpressing SW480 cells. (a) Immunoblot showing the co-immunoprecipitation of MYC-tagged delta133p53ß and endogenous RhoB. (b) RhoC activity in HCT116, SW480 and SW620 cells. Results are expressed as the fold change compared wit...
Article
Full-text available
DNA mismatch repair (MMR) protein analysis by immunohistochemistry (IHC) can identify colorectal cancer (CRC) with microsatellite instability (MSI). As MLH1-deficient CRC can be hereditary or sporadic, markers to distinguish between them are needed. MLH1 promoter methylation assay is the reference method; however, sometimes, it is challenging on fo...
Article
Activation of receptor tyrosine kinases (RTK), such as those belonging to the human epidermal growth factor receptor (HER) family, occurs only after receptor dimerization, which is a crucial step for cellular signal transduction and diversification. The HER family includes four members (EGFR/HER1, HER2, HER3, and HER4) that can homodimerize or hete...
Article
e14621 Background: Peritoneal carcinomatosis (PC) occurs in 10 to 15% of colorectal carcinoma (CRC). Strategies combining surgical resection and hyperthermic intraperitoneal chemotherapy have improved PC outcome. The morphologic, phenotypic and molecular spectrum of CRC PC and their clinical impact are still unknown. Methods: We selected 70 CRC PC...
Article
Full-text available
Following the development of targeted therapies against EGFR and HER2, two members of the human epidermal receptor (HER) family of receptor tyrosine kinases, much interest has been focused on their expression in tumors. However, knowing the expression levels of individual receptors may not be sufficient to predict drug response. Here, we describe t...
Data
TR-FRET quantification of HER dimers in 18 breast cancers. (TIF)
Data
TR-FRET quantification of EGFR and HER2 protein expression in 18 breast tumors. (TIF)
Article
Full-text available
KRAS status assessment is mandatory in patients with metastatic colorectal cancer before therapy with anti-epidermal growth factor receptor monoclonal antibodies, as KRAS mutations are associated with resistance to this treatment. However, KRAS genotyping may be very challenging in case of poor tumor cellularity, particularly when major tumor regre...
Article
Full-text available
Neoadjuvant radiochemotherapy followed by total mesorectal excision is now the standard treatment for locally advanced rectal cancer. However, tumor response to chemoradiation varies widely among individuals and cannot be determined before the final pathologic evaluation. The aim of this study was to identify germline genetic markers that could pre...
Article
Aberrant activation of the HER signaling pathways plays a critical role in the invasive and metastatic potential of tumors. The aim of this study was to address whether, in rectal cancer, alterations of these pathways could have a value as prognostic factors to be used to identify patients who are at risk of distant metastases. Therefore, the mRNA...
Article
Introduction L’oligomerisation des Recepteurs a Tyrosine-Kinase (RTKs) HER1(EGFR) et HER2 (erbB-2) est un facteur critique dans les mecanismes d’oncogenese. La surexpression de HER2, utilisee pour selectionner les patients en vue d’un traitement a l’herceptine (anti-HER2) est communement evaluee par Immunohistochimie. Cette technique n’est pas quan...
Article
Full-text available
Pancreatic carcinoma remains a treatment-refractory cancer with a poor prognosis. Here, we compared anti-epidermal growth factor receptor (EGFR) and anti-HER2 monoclonal antibodies (2mAbs) injections with standard gemcitabine treatment on human pancreatic carcinoma xenografts. Nude mice, bearing human pancreatic carcinoma xenografts, were treated w...
Article
Full-text available
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard technique for mRNA quantification, but appropriate normalization is required to obtain reliable data. Normalization to accurately quantitated RNA has been proposed as the most reliable method for in vivo biopsies. However, this approach does not correct diff...
Article
Full-text available
Gene expression analysis has many applications in the management of cancer, including diagnosis, prognosis, and therapeutic care. In this context, the reverse transcription quantitative polymerase chain reaction (RT-qPCR) has become the "gold standard" for mRNA quantification. However, this technique involves several critical steps such as RNA extr...
Article
Gene expression quantification using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) requires data normalization using an invariable reference gene. Here we assessed the stability of 15 housekeeping genes in 31 tumor and normal rectal samples to validate a reliable reference gene for rectal cancer studies. Our data show that...
Article
To investigate whether CCND1 genetic variations associated with a constitutive nuclear protein may influence either the pathologic response to preoperative RT or the prognosis in a series of rectal cancer patients. Seventy rectal cancer patients treated by neoadjuvant radiotherapy were included in the study. CCND1 exon 5 mutations were screened, an...
Article
The ability to realize simultaneous genotyping of multiple single-nucleotide polymorphisms or mutations is valuable in DNA samples from complex multigenic pathologies such as cancer. In this way, the complexity (number of hybridization units per chip) of the developed MICAM DNA chip, and the orientation of the grafted pyrrole oligonucleotides, make...
Article
Full-text available
With the increasing availability of genetic information and its relationship to human diseases, there is a growing need in the medical diagnostic field for technologies that can proceed to the parallel genotyping of multiple markers. In this paper, we report the development of a new flexible microarray-based method that aims to be inexpensive, accu...
Article
Full-text available
Background: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard technique for mRNA quantification, but appropriate normalization is required to obtain reliable data. Normalization to accurately quantitated RNA has been proposed as the most reliable method for in vivo biopsies. However, this approach does not...

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