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Citations since 2016
17 Research Items
Soon after my PhD studies, which I did in the field of genetically modified organisms, I joined a European Metrology Programme for Innovation and Research (EMPIR) project AntiMicroResist, and from then on metrology for support of nucleic acid measurements has been the central point of my studies. My expertise lies in design and evaluation of complex molecular biology methods such as dPCR and NGS complete with underlying metrological aspects that influence measurement uncertainty.
September 2015 - December 2015
RIKILT Wageningen University & Research
- NGS data analysis for developement of an automated bioinformatics pipeline for multiplex detection of unknown and unauthorised GMOs
This paper describes Food-MetNet, a coordinated preparatory initiative to establish the European Metrology Network on Food Safety (EMN-FS). Food-MetNet aims to establish a long-term ongoing dialogue between the metrology community and relevant stakeholders, in particular, European Union Reference Laboratories (EURLs), National Reference Laboratorie...
A candidate digital PCR (dPCR)-based reference measurement procedure for quantification of human cytomegalovirus (hCMV) was evaluated in 10 viral load comparison schemes (seven external quality assessment (EQA) and three additional training schemes) organized by INSTAND e.V. over four years (between September 2014 and March 2018). Four metrology in...
Antimicrobial drug resistance is one of the biggest threats to human health worldwide. Timely detection and quantification of infectious agents and their susceptibility to antimicrobial drugs are crucial for efficient management of resistance to antiviral drugs. In clinical settings, viral drug resistance is most often associated with prolonged tre...
In recent years, pepino mosaic virus (PepMV) has rapidly evolved from an emerging virus to an endemic pathogen, as it causes significant loses to tomato crops worldwide. At present, the main control strategy for prevention of PepMV disease in tomato production remains based on strict hygiene measures. To prevent damage caused by PepMV, cross-protec...
The increased use of genetically modified organisms (GMOs) is accompanied by increased complexity of the matrices that contain GMOs. The most common DNA-based approach for GMO detection and quantification is real-time quantitative polymerase chain reaction (qPCR). However, as qPCR is sensitive to inhibitors and relies on standard curves for quantif...
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
The standard-curve based simplex quantitative polymerase chain reaction (qPCR) has been the gold standard for DNA target quantification for more than a decade. The large and growing number of individual analyses needed to test for genetically modified organisms (GMOs) is reducing the cost-effectiveness of qPCR. Droplet digital PCR (ddPCR) enables a...
The majority of feed products in industrialised countries contains materials derived from genetically modified organisms (GMOs). In parallel, the number of reports of unauthorised GMOs (UGMOs) is gradually increasing. There is a lack of specific detection methods for UGMOs, due to the absence of detailed sequence information and reference materials...
Accurate and precise nucleic-acid quantification is crucial for clinical and diagnostic decisions, as overestimation or underestimation can lead to misguided treatment of a disease or incorrect labelling of the products. Digital PCR is one of the best tools for absolute nucleic-acid copy-number determination. However, digital PCR needs to be well c...
Quantifcation of genetically modifed organisms (GMOs) in food and feed products is often required for their labelling or for tolerance thresholds. Standard-curve-based simplex quantitative polymerase chain reaction (qPCR) is the prevailing technology, which is often combined with screening analysis. With the rapidly growing number of GMOs on the wo...
Background: Monoclonal antibodies (mAbs) are an important tool in diagnostics and research, especially when we are dealing with a protein marker of unknown primary structure as in the case of bilitranslocase (BTL). BTL is also expressed on kidney cells, where it acts as an organic anion transporter. We have shown earlier that there are diﬀerences i...
Presence of genetically modified organisms (GMO) in food and feed products is regulated in many countries. The European Union (EU) has implemented a threshold for labeling of products containing more than 0.9% of authorized GMOs per ingredient. As the number of GMOs has increased over time, standard-curve based simplex quantitative polymerase chain...
The main objective of BACTO-MET is to develop and evaluate candidate higher order methods for accurate measurement of Gram-negative bacteria causing nosocomial respiratory tract infection, and their susceptibility/resistance to antibiotics, and assign values of candidate calibrators and/or reference materials. The study will also investigate metrological support for new and innovative approaches that potentially offer the next generation of diagnostic solutions to revolutionise the fast diagnosis needed for timely management of disease.