Alexander M. Kudryavtsev

Alexander M. Kudryavtsev
Russian Academy of Sciences | RAS · Plant Genetics

About

81
Publications
4,516
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
439
Citations

Publications

Publications (81)
Article
Full-text available
Non-specific lipid-transfer proteins (nsLTPs) represent a family of plant antimicrobial peptides (AMPs) implicated in diverse physiological processes. However, their role in induced resistance (IR) triggered by non-pathogenic fungal strains and their metabolites is poorly understood. In this work, using RNA-seq data and our AMP search pipeline, we...
Article
The genetic diversity of 123 apple accessions from the collection of the research and production base Pushkin and Pavlovsk Laboratories of VIR, including landraces, Soviet cultivars, and commercial Russian and foreign cultivars, was for the first time estimated using the AFLP technique. A total of 355 fragments were obtained, 319 of which were poly...
Article
Full-text available
Antimicrobial peptides (AMPs) are the main components of the plant innate immune system. Defensins represent the most important AMP family involved in defense and non-defense functions. In this work, global RNA sequencing and de novo transcriptome assembly were performed to explore the diversity of defensin-like (DEFL) genes in the wheat Triticum k...
Article
S-SAP (Sequence-Specific Amplified Polymorphism) markers developed on the basis of Cassandra retrotransposon LTR sequences were first used to study the genetic diversity and phylogeny of 65 representatives of seven species of the genus Prunus L. selected from the collection of the Krymskaya Experimental Breeding Station (KEBS). The use of six S-SAP...
Article
Full-text available
At Pushkin and Pavlovsk Laboratories of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) a diverse collec tion of local apple cultivars is maintained. Some of the cultivars are widely used in breeding programs for their ecological plasticity, increased adaptation to abiotic stress and disease resistance, still there have been...
Article
Full-text available
Цитогенетическое исследование 67 образцов эфиопской пшеницы, собранных экспедицией Н.И. Вавилова в 1927 г. и через 85 лет Совместной российско-эфиопской биологической экспедицией (СРЭБЭ/ JERBE) в 2012 г. в тех же районах Эфиопии, показало сохранение системы полиморфизма гетерохроматических районов хромосом при изменении структуры популяции эфиопски...
Article
Full-text available
All the new challenges that food production has been experienced, requires adequate response not only in wide agricultural practice, but also in modern breeding programs. Broadening genetic variability is indispensable to meeting the border set by climatic changes, land erosion, human population growth, and sustainable agriculture. Gathering geneti...
Article
Full-text available
The origin, diversity and distribution of hexaploid wheat still remain somewhat unclear. In this study we examined the patterns of genetic diversity and phylogenetic relationships of seven hexaploid wheat species using integration site polymorphism of the LTR retrotransposons. Forty-eight accessions (most of them aboriginal) of seven wheat species...
Article
The Inter Simple Sequence Repeats (ISSR) analysis of 93 samples of a rare endemic species Allium regelianum, growing in Volgograd oblast is conducted for the first time. Using the six primers, 109 fragments are obtained, of which 87 (79.8%) are polymorphic. The intra- and interpopulation diversity of A. regelianum is estimated. The highest rates of...
Article
Full-text available
Sequence-Specific Amplified Polymorphism (S-SAP)-analysis of 131 accessions of different species from five sections of the genus Malus was carried out to study genetic diversity, and to clarify phylogenetic and taxonomic issues. S-SAP-markers, based on long terminal repeat (LTR)-retrotransposons TRIM2 and dem1, were developed, which identified 679...
Article
AFLP analysis of 144 samples of tetraploid wheat species, including cultivars and landraces of Triticum durum from Azerbaijan, was conducted. We obtained 249 fragments, 189 (76%) of which were polymorphic. The Dice genetic similarity index ranged from 0.67 to 0.99. Principal coordinate analysis revealed clear differences between the group of wheat...
Article
Full-text available
The sorbitol-6-phosphate dehydrogenase gene (S6PDH) sequences of eight tribe Pyreae species (Rosaceae) are studied for the first time. The exon–intron structure and polymorphism of the nucleotide and amino acid sequences of this gene are characterized. The interspecific polymorphism of the S6PDH coding sequences in the studied Pyreae species is 8.3...
Article
Full-text available
Background. Knowledge of genetic diversity within and among populations of rare and endangered plants species is practically important for conservation management. Molecular markers are useful tools for analysis of genetic diversity. In this study ISSR-analysis of rare endemic species Allium regelianum which grows in the Volgograd region was perfor...
Article
SSR analysis of rare and endemic species Allium regelianum, which grows in the south of Russia, was performed for the first time. Variability analysis of 88 accessions of A. regelianum was carried out using four highly polymorphic microsatellite loci (PIC value ranged from 0.55 to 0.72). SSR-analysis made it possible to revealed polymorphism within...
Article
Full-text available
NBS-profiling was used to study NBS-LRR resistance genes polymorphism in 32 Russian and foreign apple (M. domestica) varieties and nine Antonovka landraces. NBS-LRR resistance genes variability in the studied apple varieties was rather low. While in Antonovka landraces specific NBS-patterns were revealed, which may indicate the presence of a number...
Article
Sequence variability of the ITS spacers and 5.8S rRNA gene was examined in 11 accessions of the subfamily Scilloideae, including seven accessions of rare and endangered species Bellevalia sarmatica from Volgograd region. The intraspecific polymorphism level of the examined ITS1–5.8S–ITS2 sequence of B. sarmatica accessions constituted 1.3%. The phy...
Article
The sorbitol-6-phosphate dehydrogenase gene (S6PDH) sequences of six Malus accessions, from five different taxonomic sections, were studied for the first time. The exon-intron structure and polymorphism of the nucleotide and amino acid sequences of these genes were characterized. The intraspecific polymorphism of the S6PDH gene was assessed for the...
Article
The first molecular genetic analysis of the apple species and varieties from Russian collections with the AFLP marker system was performed in order to study the genetic diversity of the genus Malus, as well as to clarify the phylogeny and solve some systematic issues of the genus. Nienty-one apple accessions, including species from five sections of...
Article
Full-text available
This paper presents the results of identification of columnar habit in apple trees using molecular markers 29f3 and 29f1. In the genome of varieties Strela, Teleimon, Kaskad, Geizer, Zelenyi Shum, Priok-skoe, and Gotika, an insertion determining the columnar crown habit in apple trees was identified in the near-gene region of the Co locus. This ins...
Article
Full-text available
One hundred and fifty Russian and foreign winter common wheat varieties were examined by the PAGE method. A total of 70 alleles were identified at six gliadin-coding loci. It was demonstrated that 42% of varieties were heterogeneous, i.e., were represented by a number of genotypes, while 52% of varieties were homogeneous. A unique combination of gl...
Article
The sequences that encode the main functional glucosyltransferase domain of sucrose synthase genes have been identified for the first time in 14 species of the genus Malus and related species of the family Rosaceae, and their polymorphism was investigated. Single nucleotide substitutions leading to amino acid substitutions in the protein sequence,...
Article
Full-text available
Features of the analyzed allele distribution in domestic and foreign varieties are revealed on the basis of apple variety and shape polymorphism in alleles of genes associated with shelf life (Md-ACS1 and Md-ACO1) and fruit quality (MD-Exp7). Two allelic states have been revealed for the Md-ACS1 gene. With regard to the foreign selection Fuji and G...
Article
The allelic diversity at four gliadin-coding loci was studied in modern cultivars of the spring and winter durum wheat Triticum durum Desf. Comparative analysis of the allelic diversity showed that the gene pools of these two types of durum wheat, having different life styles, were considerably different. For the modern spring durum wheat cultivars...
Article
The nucleotide sequence of the ITS1-5.8S ribosomal DNA spacer fragment was determined for 41 samples of the Malus species. The total length of compared sequences ranged from 389 to 392 bp. The nucleotide sequence of the 5.8S gene within the genus was highly conserved. The level of polymorphism of ITS1 region comprised 14%. Both species- and group-s...
Article
Full-text available
The genetic diversity of strawberry F. x ananassa varieties with respect to FaRE1 LTR retrotransposon integration sites was studied for the first time. The SSAP method with a single combination of primers revealed 35 polymorphic sites of FaRE1 LTR retrotransposon integration in the strawberry genome. Most of the studied varieties (36 out of 46) had...
Article
DNA samples obtained from the populations of the Red Data Book species Bellevalia sarmatica (Georgi) Worovow from Volgograd oblast were examined using RAPD and AFLP analyses. DNA marking revealed considerable differences in the levels of interpopulation and intraspecific polymorphism of the Bellevalia. Furthermore, RAPD analysis, despite of lower l...
Article
The non-transgenic manipulation of starch properties in common wheat (Triticum aestivum L.) generally implies combining mutant alleles of the particular gene copies in all three subgenomes (A, B and D). The redundancy of the hexaploid wheat chromosome set substantially complicates the identification of recessive mutations and breeding. Nevertheless...
Article
Gliadins are seed storage proteins which are characterized by high intervarietal polymorphism and can be used as genetic markers. As a result of our work, a considerably extended catalogue of allelic variants of gliadin component blocks was compiled for durum wheat; 74 allelic variants for four gliadin-coding loci were identified for the first time...
Article
The possibility of using RAPD and AFLP methods for genetic monitoring of populations of Matthiola fragrans (Bunge), a species included in the Red Book of the USSR, was shown for the first time. An analysis of inter- and intrapopulation and interspecies genome polymorphism was performed. Differences in the genetic structure of Matthiola populations...
Article
Full-text available
The recombinant inbred line mapping population Opata85 × Synthetic W7984 was used to map Jeli long terminal repeat retrotransposon insertion sites in the hexaploid wheat genome. Sequence-specific amplified polymorphism technique was applied to reveal Jeli insertions. Jeli was found to provide multiple genetic markers for common wheat. Our marker sy...
Article
Full-text available
Molecular markers based on retrotransposon insertions are widely used for various applications including phylogenetic analysis. Multiple cases were described where retrotransposon-based markers, namely sequence-specific amplification polymorphism (SSAP), were superior to other marker types in resolving the phylogenetic relationships due to their hi...
Article
Full-text available
The polymorphism of gliadins was studied in 98 Bulgarian durum wheat (Triticum durum Desf.) landraces and classified according to the existing catalogues of blocks of gliadin components. In total, 31 alleles, including 12 new ones, were revealed for five gliadin-coding loci. Nine allele families, which included several alleles coding similar blocks...
Article
Full-text available
Contemporary trends and perspectives of Chinese winter wheat breeding programs were explored using multiple alleles of gliadin (Gli) and glutenin (Glu)-coding loci as genetic markers. To estimate genetic diversity and specific features of Chinese cultivars, the allele frequencies and genetic diversity H in Chinese wheat pool were calculated and com...
Article
Full-text available
Genetic diversity for the alleles of gliadin-coding loci was studied with 465 durum wheat accessions from 42 countries. A total of 108 alleles were identified for four loci; 60 alleles were described for the first time. Broad diversity of rare gliadin-coding alleles was observed. The highest genetic diversity was characteristic of durum wheat acces...
Article
In this work, we isolated and characterized novel antifungal proteins from seeds of dandelion (Taraxacum officinale Wigg.). We showed that they are represented by five isoforms, each consisting of two disulphide-bonded large and small subunits. One of them, To-A1 was studied in detail, including N-terminal amino acid sequencing of both subunits, an...
Article
Full-text available
Allelic diversity at five gliadin-coding gene loci has been studied in the most important spring durum wheat cultivars released in Russia and former Soviet republics in the 20th century (66 cultivars). Seven, 5, 8, 13, and 2 allelic variants of blocks of gliadin components controlled by the loci Gli-A1 d , Gli-B1 d , Gli-A2 d , Gli-B2 d , and Gli-B...
Article
Allelic diversity at five gliadin-coding gene loci has been studied in the most important spring durum wheat cultivars released in Russia and former Soviet republics in the 20th century (66 cultivars). Seven, 5, 8, 13, and 2 allelic variants of blocks of gliadin components controlled by the loci Gli-A1d, Gli-B1d, Gli-A2d, Gli-B2d, and Gli-B5d, resp...
Article
Full-text available
RAPD analysis was used to verify the varieties in an in vitro germplasm collection of lilac Syringa vulgaris L. RAPD patterns were obtained with 16 decanucleotide primers for 46 accessions (microclones and corresponding reference varieties). The RAPD patterns of a microclone and the corresponding reference variety often differed in composition; con...
Article
Sixty-four durum wheat varieties of the domestic breeding (USSR and Russia) were studied for heterogeneity using various genetic markers: storage proteins (gliadins), RAPD, and microsatellite (SSR) markers. About a third of the studied varieties (24) were shown to be heterogeneous at the protein markers. These varieties contained from two to six bi...
Article
Full-text available
Gliadin of 21 Ethiopian durum wheat genotypes representing about 95% of officially registered cultivars were studied by using acid polyacrylamide gel electrophoresis (APAGE). Analysis of gliadin electrophoretic patterns made it possible to identify 17 alleles at four main gliadin loci Gli-1 and Gli-2 (from two to six per locus) and 17 gliadin genot...
Article
Polymorphism at 28 SSR loci was analyzed and described in 45 cultivars of spring durum wheat created in the former USSR and Russia during the last 80 years. Each cultivar was shown to have a unique allele combination. This allows SSR markers to be used to identify durum wheat varieties. Meanwhile, these markers can hardly be used to detect phylogen...
Article
RAPD analysis was carried out with 22 accessions of the genus Syringa, including six species, one interspecific hybrid, and 15 cultivars. In total, 500 polymorphic fragments were detected; species-specific and cultivar-specific markers were identified. For the first time, genetic polymorphism and genome similarity coefficients were estimated and ph...
Article
Full-text available
RAPD analysis was carried out with 22 accessions of the genus Syringa, including six species, one interspecific hybrid, and 15 cultivars. In total, 512 polymorphic fragments were detected; species-specific and cultivar-specific markers were identified. For the first time, genetic polymorphism and genome similarity coefficients were estimated and ph...
Article
A comparison of similarity indices between 64 durum wheat cultivars calculated using pedigree analysis and RAPD method showed a correspondence between these two approaches to estimation of genetic diversity. The associations between the results of RAPD clustering and coefficients of parentage (chi 2 test) and the coefficient of correlation between...
Article
A comparison of similarity indices between 64 durum wheat cultivars calculated using pedigree analysis and RAPD method showed a correspondence between these two approaches to estimation of genetic diversity. The associations between the results of RAPD clustering and coefficients of parentage (2 test) and the coefficient of correlation between simi...
Article
Each wheat cultivar has a characteristic spectrum of gliadins. This makes it possible to use blocks of the components of reserve proteins as genetic markers when estimating seed quality. However, identification of the blocks that constitute the electrophoretic spectrum is a complicated task. For this purpose artificial neural network (ANN) technolo...
Article
Each wheat cultivar has a characteristic spectrum of gliadins. This makes it possible to use blocks of the components of reserve proteins as genetic markers when estimating seed purity and identity. However, identification of the blocks that constitute the electrophoretic spectrum is a complicated task. For this purpose artificial neural network (A...
Article
Gliadins of 72 Italian durum wheats representing about 80% of cultivars registered in the Italian catalogue were studied by APAG electrophoresis. Altogether, 26 different alleles were identified for the four main gliadin coding loci. Three novel alleles were identified and a catalogue of the alleles was compiled. Among the 72 cultivars, 16 were fou...
Article
Full-text available
Polymorphism and inheritance of wheat storage protein, gliadin, of durum (macaroni) and carthlicum wheats have been studied. Analysis of gliadin in 78 cultivars and in F2 seeds of intercultivar crosses of durum wheat revealed three different chromosome 1A-encoded blocks of components similar to those found in common wheat (GLD1A2, GLD1A18, GLD1A19)...
Article
The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate, pH 3.1) and two-dimensional (first dimension, Al-lactate, pH 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. Analysis of hybrids has shown that all components of the alpha zone of gliadin spectra are inherit...

Network