Alex Ignatov

Peoples' Friendship University of Russia | rudn · Faculty of Agriculture

Background and objectives Xanthomonas campestris pv. campestris (X.c.c.), the causal agent of black rot of crucifers, is one of the most important diseases of Brassica. It occurs world wide and attacks all cultivated brassicas and numerous cruciferous weeds. Control of the disease can be achieved by eliminating sources of inoculum and by the use of...

Background In recent years, the damage caused by bacterial pathogens to major crops has been increasing worldwide. Pseudomonas syringae is a widespread bacterial species that infects almost all major crops. Different P. syringae strains use a wide range of biochemical mechanisms, including phytotoxins and effectors of the type III and type IV secre...

Agriculture is one of the backbone sectors of national economics and a factor of political stability in many countries. International organizations (IPPC, FAO) estimate that up to 40% of annual yield with the cost of about 88 bill. U.S. dollars is lost due to the pests and diseases. Thus, prevention of plant pathogen dissemination is an internation...

Curtobacterium is a genus of Gram-positive bacteria within the order Actinomycetales. Some Curtobacterium species (C. flaccumfaciens, C. plantarum) are harmful pathogens of agricultural crops such as soybean, dry beans, peas, sugar beet and beetroot, which occur throughout the world. Bacteriophages (bacterial viruses) are considered to be potential...

Curtobacterium flaccumfaciens pv. flaccumfaciens (H.) Collins & Jones is known as a pathogen of different legume crops, including soybean (Glycine max (L.) Merr.) (Hedges 1922; Dunleavy 1983). OEPP/EPPO (2011) considers C. flaccumfaciens pv. flaccumfaciens as present in Russia based on reports of the disease on common beans in two regions of Russia...

In the summer of 2018, wilt and leaf spots were observed on sunflower (Helianthus annuus L.) plants in fields near Kursk (51.74°N, 36.02°E) in Russia. In the following years, incidence of this disease was 5 to 20% in the inspected fields. Marginal chlorosis on seedling leaves developed into wilt and necrosis about one week later (Fig. 1). Mature pl...

Abstract Proteobacteria comprising species of Pseudomonas syringae group cause diseases of many plants around the world. The phytopathogen has a complex taxonomic structure, which is constantly being revised due to the emergence of new molecular and biochemical diagnostic methods. Here for the first time, we describe the genetic and phenotypic dive...

Database of genetic collection of bacterial plant pathogens of genus Xanthomonas isolated in Russia

Novel, closely related phages Possum and Horatius infect Pectobacterium versatile, a phytopathogen causing soft rot in potatoes and other essential plants. Their properties and genomic composition define them as N4-like bacteriophages of the genus Cbunavirus, a part of a recently formed family Schitoviridae. It is proposed that the adsorption appar...

Diseases caused by the Gram-positive bacterium Curtobacterium flaccumfaciens pv. flaccum-faciens (Сff) inflict substantial economic losses in soybean cultivation. Use of specific bacterial viruses (bacteriophages) for treatment of seeds and plants to prevent the development of bacterial infections is a promising approach for bioprotection in agricu...

Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot of crucifers, remains a threat to all Brassica crops around the world for almost 140 years [3, 7, 9]. Bacteria of the genus Xanthomonas cause diseases on over 500 plant species [6]. Recent genomic and genetic data improved our understanding of Xcc taxonomy and virulence mech...

The spread of bacterial pathogens of sugar beet was assessed by visual examination, PCR and microbiological/ phytopathological diagnostics of the collected at 2017 and 2020 plants samples. A high initial degree of damage to sugar beet plants by the causative agent of bacterial spot and blight Pseudomonas syringae pv. aptata has been established, an...

Since 2011, bacterial tail rot of sugar beet roots has spread widely in the Black-Earth Region leading to great losses, especially during sugar beet storage. The symptoms, prevalence, and favorable conditions for the disease development are described. The dynamics of changes in species composition of the disease agents where the dominant patho...

Bacterial viruses (bacteriophages) have been considered as potential agents for the biological control of bacterial phytopathogens due to their safety and host specificity. Pseudomonas savas-tanoi pv. glycinea (Psg) is a causative agent of the bacterial spotting of soybean (Glycine max Willd). The harm caused by this bacterium to crop production an...

The genus of Curtobacterium, belonging to the Microbacteriaceae family of the Actinomycetales order, includes economically significant pathogenic bacteria of soybeans and other agricultural crops. Thorough phylogenetic and full-genome analysis using the latest genomic data has demonstrated a complex and contradictory taxonomic picture within the gr...

Black rot (caus. agent Xanthomonas campestris pv. campestris (Dowson) Dye, et al. (1980) is the most harmful disease of brassicas, widespread across the World. Plant breeding for resistance to Xcc received a new push after the discovery of a race-specific reaction in several plant species of the Brassicaceae family in 1992 and, accordingly, the rac...

The throughput and single-base resolution of RNA-Sequencing (RNA-Seq) have contributed to a dramatic change in diagnostics of viruses and other plant pathogens. A transcriptome represents all RNA molecules, including the coding mRNAs as well as the noncoding rRNA, tRNA, etc. A distinct advantage of RNA-Seq is that cDNA fragments are directly sequen...

The throughput and single-base resolution of RNA-Sequencing (RNA-Seq) have contributed to a dramatic change in diagnostics of viruses and other plant pathogens. A transcriptome represents all RNA molecules, including the coding mRNAs as well as the noncoding rRNA, tRNA, etc. A distinct advantage of RNA-Seq is that cDNA fragments are directly sequen...

Pectobacterium parmentieri is a plant-pathogenic bacterium, recently attributed as a separate species, which infects potatoes, causing soft rot in tubers. The distribution of P. parmentieri seems to be global, although the bacterium tends to be accommodated to moderate climates. Fast and accurate detection systems for this pathogen are needed to st...

Genetic collection of Solanaceous bacterial pathogens, database.

The study of the ecological and evolutionary traits of Soft Rot Pectobacteriaceae (SRP) comprising genera Pectobacterium and Dickeya often involves bacterial viruses (bacteriophages). Bacteriophages are considered to be a prospective tool for the ecologically safe and highly specific protection of plants and harvests from bacterial diseases. Inform...

Characteristic leaf spot and blight symptoms caused by Robbsia andropogonis on bougainvillea plants were found in three locations in different provinces of Mexico from 2019 to 2020. Eleven bacterial isolates with morphology similar to R. andropogonis were obtained from the diseased bougainvillea leaves. The isolates were confirmed as R. andropogoni...

Plant pathogenic xanthomonads virulent to wheat, rye, barley, tomato, sunflower, and brassicas were isolated in Russia in 2001–2008. Physiological tests and multilocus sequence typing analysis confirmed their position within the Xanthomonas arboricola species. The obtained draft genome sequence of representative strain 3004 from barley plants, whic...

A pathovar flaccumfaciens of gram-positive bacterium Curtobacterium flaccumfaciens (Cff) is the causal agent of bacterial wilt of Leguminosae plants, a disease that reduces the yields of many economically important crops around the globe. Previous genomic studies demonstrated the differences between pathogenic Curtobacterium flaccumfaciens pv. flac...

Abstract Relevance. Lettuce (Latin: Lactúca satíva) is a species of annual herbaceous plant in the genus Lettuce of the Asteraceae family. As a vegetable crop, it is cultivated everywhere in the world, and its hydroponic cultivation technology has received special development in recent years. One of the common pathogens of lettuce is Pseudomonas ci...

Black rot of brassicas (caus. agent Xanthomonas campestris pv. campestris – Xcc) is one of the most devastating diseases of vegetables. Previous comparison of Xcc strains collected before 1998 demonstrated distinct reactions for strains recovered from plants of subtropical (group 1) and temperate climatic zones (group 2) against antibodies reacting...

Genetic collection of Pseudomonas syringae from diverse crops collected at Russian State Agrarian University - MSKHA.

Using bacteriophages (bacterial viruses) to control pathogenic bacteria is a promising approach in horticulture. However, the application of this strategy in real conditions requires compliance with particular technological and environmental restraints. The presented paper concerns the process of phage selection to create a cocktail that is efficie...

The causative agent of the pandemic identified as SARS-CoV-2 leads to a severe respiratory illness similar to SARS and MERS with fever, cough, and shortness of breath symptoms and severe cases that can often be fatal. In our study, we report our findings based on molecular docking analysis which could be the new effective way for controlling the SA...

Availability of ample genomic data promotes the reevaluation and reestablishment of bacterial taxonomy and evolution. One of taxons under active study is Pectobacteriaceae, a family of Gram-negative bacteria that cause diseases of important agricultural plants. Elucidation of genomic and transcriptomic features of bacterial species facilitates the...

Availability of ample genomic data promotes the reevaluation and reestablishment of bacterial taxonomy and evolution. One of taxons under active study is Pectobacteriaceae, a family of Gram-negative bacteria that cause diseases of important agricultural plants. Elucidation of genomic and transcriptomic features of bacterial species facilitates...

The keystone difference between viruses and other domains of life is an absence of own translation apparatus in viruses. However, the rapidly accumulating amount of genomic information indicates that a noticeable number of viral genomes contain sequences related to ribosomal RNA. In order to clarify the conservatism and possible evolutionary pathwa...

The causative agent of the viral pneumonia outbreak in the world identified as SARS-CoV-2 leads to a severe respiratory illness like SARS and MERS. The pathogen spreading has turned into a pandemic dissemination and increased the mortality rate. Therefore, any useful information is essential for effective control of the disease. Our findings show t...

The recent taxonomic diversification of bacterial genera Pectobacterium and Dickeya, which cause soft rot in plants, focuses attention on the need for improvement of existing methods for the detection and differentiation of these phytopathogens. This research presents a whole genome-based approach to the selection of marker sequences unique to part...

Blackleg and soft rot of potato (Solanum tuberosum) were monitored in the Central European part of Russia within a period of 2012- 2019. Symptoms included decay of tubers, blackening of stem vascular bundles, and partial yellowing of leaves. The disease causes serious potato yield losses in the field and storage. Pectobacterium parmentieri, P. bras...

Одна из важнейших продовольственных культур в мире, картофель (Solanum tuberosum L.) заражается многими вирусами, из которых вирус Y (Potato virus Y, PVY) имеет наиболее важное экономическое значение, вызывая существенные потери урожая [1]. Эффективных препаратов для защиты растений от вирусов практически не существует. Экзогенные дцРНК которые рас...

Представленная вниманию читателя книга суммирует пяти- летний опыт работы лаборатории молекулярной биоинженерии Института биоорганической химии им. академиков М. М. Шемя- кина и Ю. А. Овчинникова РАН над поддержанным Российским Научным Фондом проектом применения вирусов бактерий (бак- териофагов, фагов) для биологического контроля бактериальных заб...

Possible tendencies in the distribution and harmfulness of phytopathogens in the world under different scenarios of climate change are reviewed. Effects of enhanced temperature, carbon dioxide and ozone concentrations separately and in combination on the phytopathogen’s distribution and harmfulness are analyzed.

One of the most important food crops in the world, the potato (Solanum tuberosum L.) is infected with many viruses, of which the y virus (Potato virus Y, PVY) is the most important economically, causing significant crop losses. Several alternative methods of dsRNA delivery have been tested, with the most promising being spray - induced gene silenci...

Twenty-one isolates of bacteriophages specific to eleven target strains of Xanthomonas campestris pv. campestris were isolated from soil samples collected under black rot-infected cabbage plants. After the analysis of phagotyping for seventy-three phytopathogen strains against newly isolated isolates and four collection strains of bacteriophages, i...

This Special Issue of BMC Microbiology “Systems Biology and Bioinformatics” presents the materials discussed at the 11-th Young Scientists School “Systems Biology and Bioinformatics”-2019 (SBB-2019) in Novosibirsk, Russia (http://conf.bionet.nsc.ru/sbb2019/en/). These Young Scientists’ Schools on bioinformatics have been organized every year since...

Bacterial blight is one of most harmful diseases of legumes, reducing the profitability of soybean production in Russian Federation. Among a number of Pseudomonas isolates obtained from diseased seeds and plants of soybean, 4 strains were selected and confirmed as Pseudomonas savastanoi pv. glycinea (Psg). Properties of the isolated bacteria were s...

Black leg and soft rot are devastating diseases causing up to 50% loss of potential potato yield. The search for, and characterization of, bacterial viruses (bacteriophages) suitable for the control of these diseases is currently a sought-after task for agricultural microbiology. Isolated lytic Pectobacterium bacteriophages Q19, PP47 and PP81 posse...

Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is a Gram-positive phytopathogenic bacterium attacking leguminous crops and causing systemic diseases such as the bacterial wilt of beans and bacterial spot of soybeans. Since the early 20th century, Cff is reported to be present in North America, where it still causes high economic losses. Cur...

Soft rot Pectobacteriaceae (SRP) causing soft rot, aerial stem rot, and black leg diseases of potatoes and vegetables pose serious economic threat to agriculture World wide. In this work we have studied phylogenetic relations within the genera Pectobacterium and Dickeya applying a full genome comparison approach. Phylogenetic trees were constructed...

100 семейств растений (84-двудольных), плодовых, ягодных и декоративных культурах, поражаемых бактериальным раком (англ.: "crown gall"-корончатый галл), вызываемым Agrobacterium tumefaciens и бактериями близких видов и даже родов альфа-протобактерий, например, Allorhizobium vitis. Приведены сведения по таксономическому положению патогена, симптомат...

Анализ наиболее вредоносных вирусных фитопатогенов на предприятиях защищенного грунта показывает, что проникновение новых видов с семенами и готовой продукцией из других регионов и стран подвергает производство томата большому риску. Главная проблема выявления заражения длительный инкубационный период для вирусных болезней и необходимость инструмен...

Phytopathogenic bacteria belonging to the Pectobacterium and Dickeya genera (soft-rot Pectobacteriaceae) are in the focus of agriculture-related microbiology because of their diversity, their substantial negative impact on the production of potatoes and vegetables, and the prospects of bacteriophage applications for disease control. Because of nume...

The survival of bacteriophage in the phyllosphere is influenced by many factors, including sunlight UV irradiation. The effect of the UV light factor on persistence of the phage mixed with different UV-protectors was evaluated in vitro and in planta on young cabbage plants in greenhouse. The exposition of UV irradiation positively correlated with p...

Plant pathogenic bacteria of the genus Xanthomonas display high levels of genetic diversity and cause remarkable damage to about 400 plant species. In 2001–2008, a new group of strains of Xanthomonas arboricola has been found as pathogens on novel host plants such as wheat, rye, barley, tomato, sunflower, and brassicas in Russia. Physiological test...

Global climate warming and involvement of new regions with endemic populations of microorganisms in commercial seed production have led to an increase in the diversity of phytopathogenic bacteria that are affecting major crops, including the fruit trees. As a rule, emergence of new pathogens is associated with importation of infected seeds and plan...

There is a description for the method for development of bacteriophage agent to control bacterial blight of soybean caused by Pseudomonas savastanoi pv. glycinea. Biological effectiveness of bacteriophage application was about 75% that was approximately the same as for test chemical bactericides.

Soft rot caused by numerous species of Pectobacterium and Dickeya is a serious threat to the world production of potatoes. The application of bacteriophages (bacterial viruses) to combat bacterial infections in medicine, agriculture, and the food industry requires the selection of comprehensively studied lytic phages and the knowledge of their infe...

Картофель находится на четвертом месте среди продовольственных культур в мире, и является одним из основных продуктов питания населения России. Высокая заболеваемость картофеля вирусными, бактериальными, оомицетными и грибными болезнями серьезно влияет на урожайность культуры, приводя также к экономическим потерям изза выбраковки семенного картофел...

Cruciferous weeds can serve as a major reservoir of inoculum of Xanthomonas campestris pv. campestris (Xcc). Surveys of Cruciferous weeds have shown black rot (BR) to be common among cruciferous weeds in central coastal non-cultivated areas of California. In California atypical leaf spotting strains of BR were found along the central coast in sever...

Xanthomonas campestris pv. campestris (Xcc) is one of devastating diseases of Brassica vegetables. We have collected Xcc isolates in the main areas of brassicas cultivation in Russia from 2006 to 2018. Multilocus sequence typing based on the partial sequence of loci dnaK, fuyA, rpoD, gyrB, cytP450, and avrXcc2109 was applied to over 100 strains rep...

Nanoparticles (NPs) have a number of unique properties associated with their ultra small size and applied delivery of polypeptides, RNA and DNA of various sizes into the plant cells. Our data demonstrate a potential of delivery of antimicrobial peptide biomolecules that have been previously isolated from wild species and structure-functional charac...

Phages of the phytopathogenic Pectobacteriaceae species causing black leg and soft rot of potato were investigated. These phages are promising as biocontrol agents to prevent the loss of seed and ware potato tubers. The present work characterizes a new podovirus PP16, infecting a broad range of Pectobacterium carotovorum strains. Based on its genom...

Картофель (Solanum tuberosum L.) – одна из наиболее важных продовольственных культур в мире. Высокая заболеваемость картофеля вирусными болезнями серьезно влияет на урожайность культуры, приводя к экономическим потерям из-за выбраковки семенного картофеля и увеличения потерь при хранении. Вирус картофеля Y (Potato Virus Y, PVY) – наиболее распростр...

Бактериозы являются одной из острых проблем сельскохозяйственного производства в современном мире. Причинами этого служат передача бактериальных патогенов с семенами и посадочным материалом, сложность правильной идентификации патогенов, отсутствие эффективных разрешенных бактерицидов и ограниченное число устойчивых к бактериозам сортов растений. Фи...

Twenty-one isolates of bacteriophages specific to eleven target strains of Xanthomonas campestris pv. campestris were isolated from soil samples collected under black rot-infected cabbage plants. An electron microscopic study of bacteriophage morphology allowed to classify 3 isolates as members of the Siphoviridae family, and the remaining eighteen...

Бактериофаги пектобактерий, вызывающих заболевания картофеля (черную ножку и мягкую гниль), – сравнительно малоизученная группа фагов, которые имеют перспективы для технологических приложений в целях предотвращения потерь товарного и семенного картофеля. В настоящей статье представлена характеристика нового подовируса PP16, инфицирующего широкий сп...

Genetic diversity of black rotpathogen in Russia / A. T. Orynbayev, N. Ha. Vo Thi, F. S. U. Dzhalilov, A. N. Ignatov // 4th International youth conference "Perspectives of science and education", New York, 10 декабря 2018 года. – New York: SLOVO\WORD, 2018. – P. 16-21. – EDN VXXJBM. Xanthomonas campestris pv. campestris (Xcc) is the causal agent o...

Dickeya solani, a recently emerged virulent bacterial potato pathogen, is a major threat to world agriculture. Because of the growing limitations in antibiotic use, the alternative strategies of bacteriosis control are considered, including the application of bacteriophages. Myoviridae bacteriophages recently re-ranked as a separate family Ackerman...

BACILLUS AMYLOLIQUEFACIENS STRAIN WITH ANTIBACTERIAL AND FUNGISTATIC ACTIVITY, AND MICROBIOLOGICAL PREPARATIONS ON ITS BASE AGAINST PLANT DISEASES CAUSED BY PHYTOPATHOGENIC MICROORGANISM (57) Abstract: FIELD: biotechnology. SUBSTANCE: claimed group of inventions includes the Bacillus amyloliquefaciens Ab8b (VKPM B-12792) bacterial strain, which has...

15 августа 2018 года в с. Рогачево Дмитровского района Московской области состоялся Картофельный Форум 2018, посвященный перспективным технологиям и инновациям в агроиндустрии. В рамках Форума прошла научно-практическая конференция «Система CRISPR-Cas в редактировании геномов картофеля и других сельскохозяйственных культур» и были представлены резу...