Alex Brogan

Alex Brogan
King's College London | KCL · Department of Chemistry

PhD

About

17
Publications
1,705
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
518
Citations
Introduction
Lecturer in the Department of Chemistry, King's College London. My main research interests are: Biocatalysis in ionic liquids, Nonaqueous enzymes, Biophysics of proteins in unnatural environments, Biomaterials, Functional hybrid materials, Protein engineering, and ionogels.
Additional affiliations
June 2019 - present
King's College London
Position
  • Lecturer
Description
  • Extreme Biomaterials
September 2015 - July 2016
Imperial College London
Position
  • Research Associate
Description
  • Lead tutorials for 4 groups of first year undergraduates (Chemistry for Chemical Engineers)
September 2014 - May 2019
Imperial College London
Position
  • Research Associate
Description
  • Enzyme stability in ionic liquids and novel ionic materials
Education
September 2008 - July 2012
University of Bristol
Field of study
  • Chemistry
September 2004 - July 2008
University of Bristol
Field of study
  • Chemistry

Publications

Publications (17)
Article
The interaction between proteins and hydration water stabilizes protein structure and promotes functional dynamics, with water translational motions enabling protein flexibility. Engineered solvent-free protein-polymer hybrids have been shown to preserve protein structure, function, and dynamics. Here, we used neutron scattering, protein and polyme...
Article
Ionogels are emerging as soft materials with remarkable physical properties that can be tuned to suite application requirements. The liquid component — ionic liquids — are effectively involatile, which provides new opportunities to explore gel surfaces with UHV based analytical techniques. Here, we exploit the highly solvating nature of ionic liqui...
Article
Full-text available
Chemical modification of proteins to yield solvent-free liquid proteins has been demonstrated to be a robust method for stabilizing proteins against temperature, aggregation, and non-aqueous environments. This new class of biomaterial shows exceptional compatibility with ionic liquids, with enzyme architecture preserved and its activity enhanced. T...
Article
Full-text available
The temperature sensitivity of vaccines and therapeutic proteins forces the distribution of life‐saving treatments to rely heavily on the temperature‐controlled (usually 2–8°C) supply and distribution network known as the cold chain. Here, using avidin as a model, we demonstrate how surface engineering could significantly increase the thermal stabi...
Article
Full-text available
Ionic liquids offer exciting possibilities for biocatalysis as solvent properties provide rare opportunities for customizable, energy-efficient bioprocessing. Unfortunately, proteins and enzymes are generally unstable in ionic liquids and several attempts have been made to explain why; however, a comprehensive understanding of the ionic liquid–prot...
Article
Ionogels are an emerging class of soft material with exceptional properties stemming from high ionic liquid content. In contrast to other gel systems, the ionic liquid component provides an extra level of design. However, this highly modular nature has yet to be fully explored and the role ionic liquids play in the structural properties of gel-base...
Article
Here, we demonstrate a chemical modification strategy to create biomaterials of the M13 bacteriophage with extraordinary thermal stability, and high compatibility with non-aqueous ionic liquids. The results provide a blueprint...
Article
Full-text available
The increasing requirement to produce platform chemicals and fuels from renewable sources means advances in biocatalysis are rapidly becoming a necessity. Biomass is widely used in nature as a source of energy and as chemical building blocks. However, recalcitrance towards traditional chemical processes and solvents provides a significant barrier t...
Article
Nonaqueous biocatalysis is rapidly becoming a desirable tool for chemical and fuel synthesis in both the laboratory and in industry. Similarly, ionic liquids are an increasingly popular anhydrous reaction media for a number of industrial processes. Consequently, the use of enzymes in ionic liquids as efficient, environment-friendly, commercial bioc...
Article
Solvent-free liquid proteins are a new class of thermally stable hybrid bionanomaterials that are produced by extensive lyophilisation of aqueous solutions of protein-polymer surfactant nano-conjugates followed by thermal anneal-ing. The hybrid constructs, which consist of a globular protein core surrounded by a monolayer of electrostatically cou-p...
Article
Water molecules play a number of critical roles in enzyme catalysis, including mass transfer of substrates and products, nucleophilicity and proton transfer at the active site, and solvent shell-mediated dynamics for accessing catalytically competent conformations. The pervasiveness of water in enzymolysis therefore raises the question concerning w...
Article
An anisotropic glucose oxidase-polymer surfactant nano-conjugate is synthesized and shown to exhibit complex temperature-dependent phase behavior in the solvent-free state. At close to room temperature, the nano-conjugate crystallizes as a mesolamellar soft solid with an expanded interlayer spacing of ca. 12 nm and interchain correlation lengths co...
Article
Redox responses associated with the heme prosthetic group in a myoglobin-polymer surfactant solvent-free liquid are investigated for the first time in the absence of an electrolyte solution. Cyclic voltammograms from the biofluid exhibit responses that are consistent with planar diffusion of mobile charges in the melt. Temperature-dependent dynamic...
Article
The thermal denaturation of solvent-free liquid lysozyme at temperatures in excess of 200°C was studied by synchrotron radiation circular dichroism spectroscopy. Temperature-dependent changes in secondary structure were used to map the equilibrium denaturation pathway and characterize a reactive β-sheet-rich unfolding intermediate that was stable i...
Article
The observation of biological activity in solvent-free protein-polymer surfactant hybrids challenges the view of aqueous and nonaqueous solvents being unique promoters of protein dynamics linked to function. Here, we combine elastic incoherent neutron scattering and specific deuterium labeling to separately study protein and polymer motions in solv...
Article
Isolating solvent effects by studying proteins in a liquid phase devoid of solvent has not been previously possible because freeze-dried protein solids do not melt but thermally degrade. Herein we circumvent this problem by modifying the interactions between myoglobin molecules via a polymer-surfactant coronal layer to produce a solvent-free liquid...
Article
Full-text available
The ensemble of forces that stabilize protein structure and facilitate biological function are intimately linked with the ubiquitous aqueous environment of living systems. As a consequence, biomolecular activity is highly sensitive to the interplay of solvent-protein interactions, and deviation from the native conditions, for example by exposure to...

Network

Cited By

Projects

Projects (3)
Project
Investigating the structure-property relationship of ionic liquid gel systems, for prospective uses in biocatalysis, biosensing, and functional soft materials.
Project
To perform biocatalysis in nonaqueous media, particularly ionic liquids.