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To create various laboratory and experimental models corresponding to artificial cells as part of a Horizon 2020 funded project called ACDC. This project includes controlled assembly and disassembly of self-assembled structures such as emulsion droplets and liposomes. This work will employ microfluidics as a technical platform.
December 2016 - July 2018
Regional Center for Diagnostic, Prognostic and Predictive Biomarkers (CRIBT)
Objective To compare the effects of the complex triamcinolone acetonide-hydroxypropyl-β-cyclodextrin (TA-CD) on in vitro inflamed primary human articular chondrocytes in the presence or absence of the mixture hyaluronic acid-Chitlac, a lactose-modified chitosan (HA-CTL). Design Changes in cell viability and pro-inflammatory cytokines gene expressi...
Conventional measurements of fasting and postprandial blood glucose levels investigated in genome-wide association studies (GWAS) cannot capture the effects of DNA variability on ‘around the clock’ glucoregulatory processes. Here we show that GWAS meta-analysis of glucose measurements under nonstandardized conditions (random glucose (RG)) in 476,32...
We simulate the movement and agglomeration of oil droplets in water under constraints, using a simplified stochastic-hydrodynamic model. We analyze both local and global properties of the networks formed by the agglomerations of droplets for various system sizes. We focus on the differences of these properties for monodisperse and polydisperse syst...
Experimentally, periodically released droplets in systems of widening pipes show clustering. This is surprising, as purely hydrodynamic interactions are repulsive so that agglomeration should be prevented. In the main part of this paper, we investigate the clustering of droplets under the influence of phenomenological hydrostatic forces and some hy...
Within the scope of the European Horizon 2020 project ACDC – Artificial Cells with Distributed Cores to Decipher Protein Function, we aim at the development of a chemical compiler governing the three-dimensional arrangement of droplets, which are filled with various chemicals. Neighboring droplets form bilayers with pores which allow chemicals to m...
We aim at planning and creating specific agglomerations of droplets to study synergic communication using these as programmable units. In this paper, we give an overview of preliminary obstacles for the various research issues, namely of how to create droplets, how to set up droplet agglomerations using DNA technology, how to prepare them for confo...
The development of data science, the increase of computational power, the availability of the internet infrastructure for data exchange and the urgency for an understanding of complex systems require a responsible and ethical use of computational models in science, communication and decision-making. Starting with a discussion of the width of differ...
We explain how to optimize the image analysis of mixed clusters of red and green droplets in solvents with various degrees of sharpness, brightness, contrast and density. The circular Hough Transform is highly efficient for separated circles with reasonable background contrast, but not for large amounts of partially overlapping shapes, some of them...
Homeostatic control of blood glucose requires different physiological responses in the fasting and post-prandial states. We reasoned that glucose measurements under non-standardised conditions (random glucose, RG) may capture diverse glucoregulatory processes more effectively than previous genome-wide association studies (GWAS) of fasting glycaemia...
Glycation is one of the most important post-translational modifications in cells and tissues and gives rise to highly reactive species called advanced glycation end products (AGEs). AGEs exert their pathological effects through different ways and previous reports suggest that they may also affect adipose tissue function and insulin sensitivity. All...
β-caryophyllene (BCP), a plant-derived sesquiterpene, has been reported to have anti-inflammatory and antioxidant effects. The purpose of this study is to evaluate the effects of BCP in combination with ascorbic acid (AA) and d-glucosamine (GlcN) against macrophage-mediated inflammation on in vitro primary human chondrocytes. Changes in cell viabil...
A Correction to this paper has been published: https://doi.org/10.1038/s41467-021-21276-3
Differences between sexes contribute to variation in the levels of fasting glucose and insulin. Epidemiological studies established a higher prevalence of impaired fasting glucose in men and impaired glucose tolerance in women, however, the genetic component underlying this phenomenon is not established. We assess sex-dimorphic (73,089/50,404 women...
Gastric cancer is the fourth most common worldwide cause of cancer-related death. Early gastric cancer has no associated symptoms, for this reason, patients come to the attention of the clinicians only in advanced stages. This paper aims to give a global view on the biomarkers for gastric cancer and the therapy in use. We discuss VEGF family, HER f...
I am looking for a bit of advice about solubility of fluorescent lipids in oil (DEP or mineral oil).
my protocol to create an oil+lipids mixture says (at least) 5 cycles of heating 10min at 80C and 10 min of sonication.
after that, the mixture is perfectly homogeneous...BUT you will not find any slightest trace of fluorescence coming from the fluorophore.
The worst result comes from DOPE PEG(2000)-N-Cy5; but also NBD-EggPE gives problems and so on other fluorophores.
(I need to work with at least two well-separated colors. )
if I add it to the final lipid-oil mixture only with vortexing, it will not mix at all, and - on top - chloroform will not evaporate and its remints are damaging for the droplets/vesicle creation.
What shall I do?
thank you, have a good one
I need a piece of advice on this topic:
I am working with oil in water droplets decorated with a fluorescent tag and ssDNA
To avoid droplets' coalescence I need to add a surfactant in the water, the SDS (0.5-1%) works well in that.... the back of the coin is that it acts as a quencher on my fluorescent tag.
does anybody of you have experience in this phenomenon?
thank you in advance
I have a problem with hFOB 1.19 cells.
They are really slow to multiplicate and don't attach properly to the plastic.
After almost a month they didn't reach confluence in t75 flask, I changed medium twice/trice a week, but each time I found a lot of cells were floating.