Aleksandra Piechota-Polanczyk

Aleksandra Piechota-Polanczyk
Jagiellonian University | UJ · Department of Medical Biotechnology

PhD

About

77
Publications
32,831
Reads
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1,842
Citations
Introduction
Received research projects: - “Role of heme oxygenase 1 in the development and progression of abdominal aortic aneurysm” founded by National Science Centre (Poland; Grant No. DEC-2015/16/S/NZ4/00040) - “Fibrosis or senescence - why transcriptionally inactive Nrf2 protects against colon dysfunction” founded by National Science Centre (Poland; Grant No. 2018/31/D/NZ4/00077) Used techniques: - In vitro and in vivo models related to AAA and IBD - Histology, IF and IHC - Gene expression analysis
Additional affiliations
November 2015 - October 2021
Jagiellonian University
Position
  • Professor (Associate)
Description
  • https://www.nicheworks.eu/copy-of-the-biology-of-heme
December 2013 - April 2015
Medical University of Łódź
Position
  • Researcher
Description
  • employed in the Healthy Aging Research Center financed by EU grant A specialist in flow cytometry (LSR II Fortessa, BD). In vitro - PBMC, HNEC, HBEC; infecting cells wit viruses; protein analysis (electrophoresis, WB , ELISA); RNA, PCR, RT-PCR, qRT-PCR
June 2013 - December 2013
University of Lodz
Position
  • Researcher
Description
  • mice models of gastrointestinal tracks diseases, research analysis at protein and gene levels using WB, PCR, RT-PCR techniques. More on http://jakubfichna.wix.com/zakladbiochemii
Education
October 2007 - October 2011
Medical University of Łódź
Field of study
  • medical biology
September 2003 - September 2007
Medical University of Łódź
Field of study
  • public health

Publications

Publications (77)
Article
Background and aim The proper functioning of the gastrointestinal system relies on an intricate crosstalk between a plethora of cell types and signaling pathways. Recently we identified that the lack of NRF2 transcriptional activity (NRF2 tKO) triggers significant colon microscopical alterations, still they do not affect the general functioning of...
Article
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Air pollution is a global problem that is responsible for more than four million premature deaths each year. Air exchange in ammonia engine rooms is a priority for normal operating conditions, as well as in the event of an emergency release. A numerical approach with the use of computational fluid dynamics techniques can provide detailed data, such...
Article
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Our recent findings indicate that Nrf2 transcriptional activity is essential in maintaining the proper large intestinal structure in adult mice. Here, we aimed to verify whether Nrf2-related intestine abnormalities stemmed from the early weaning or gestational periods. Therefore, we analyzed 4-day-old pups and embryos devoid of Nrf2 transcriptional...
Article
Background and objectives: Endovascular prosthesis placement is a predominant surgical procedure to reduce the risk of aneurysm rupture in abdominal aortic aneurysm patients. The formation of an endoleak is a major complication of stent-graft placement. Therefore, the aim of this study was to determine the influence of stent-graft’s spatial configu...
Article
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Fires in landfills, where used plastic packaging waste is discarded, have shown how great a fire hazard these types of materials pose. In this study, the course of thermo-oxidation of samples made of polypropylene (PP), polystyrene (PS), and polyethylene terephthalate (PET) based plastics was determined. Based on an analysis of the dissociation ene...
Article
Contamination of toxic and odorous gases emitted from stacks in buildings located in an urban environment are potential health hazards to citizens. A simulation using the computational fluid dynamic technique may provide detailed data on the flammable region and spatial dispersion of released gases. Concentrations or emissions associated with garag...
Article
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Using computer tomography angiography (CTA) and computational structural analysis, we present a non-invasive method of mass flow rate/velocity and wall stress analysis in type B aortic dissection. Three-dimensional (3D) computer models of the aorta were calculated using pre-operative (baseline) and post-operative CT data from 12 male patients (aged...
Article
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Smoke from fires in residential buildings represents the greatest threat to the life and health of inhabitants and firefighters at the scene of an accident. Therefore, the aim of this study was to reconstruct a numerical model for the estimation of smoke spread in a medium-high building under different ventilation conditions. Here, the three-dimens...
Article
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The storage of large numbers of batteries and accumulators is associated with an increased risk of their ignition, which results in the release of significant amounts of hydrogen into the environment. The aim of the study was to reconstruct hazardous zones after hydrogen and liquefied propane–butane (reference gas) release for different industrial...
Article
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Abdominal aortic aneurysm (AAA) bears a high risk of rupture and sudden death of the patient. The pathogenic mechanisms of AAA remain elusive, and surgical intervention represents the only treatment option. Heme oxygenase-1 (HO-1), a heme degrading enzyme, is induced in AAA, both in mice and humans. HO-1 was reported to mitigate AAA development in...
Article
LPG storage tanks may be seriously threatened by a fire coming from nearby fuels or by leakage appearance. The aim of the study was to prepare a three-dimensional model of LPG release on a car gas station under different environmental conditions. CFD simulations of liquid and gas phase release from a tank localized on a car gas station was performe...
Article
Human activities such as transport contribute to the environmental degradation. Therefore, the aim of the study was to determine the level of contamination of soils, from different districts of big cities of central Europe, with heavy metals: zinc, lead, and nickel. The samples were taken from the top, sodded soil layer. Each single sample weigh wa...
Article
The influence of sun rays, wind speed, and different type of subsoil on the evaporation process was analyzed. A dedicated experimental set-up for investigation of evaporation process of three liquids (ethanol, petrol and tap water) deposited on glass and sand was created. Results indicated that for porous surfaces wind decreased the amount of evapo...
Article
Nrf2 (nuclear factor erythroid 2-related factor 2) is a stress-responsive transcription factor, associated with cellular homeostasis. Under normal conditions Nrf2 is kept in the cytoplasm by Kelch-like ECH-associated protein 1 (Keap1) which facilitates its degradation. Meanwhile, oxidative or electrophilic stress trigger Keap1 dissociation from the...
Article
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The aim of this study was to prepare a self-made mathematical algorithm for the estimation of risk of stent-graft migration with the use of data on abdominal aortic aneurysm (AAA) size and geometry of blood flow through aneurysm sac before or after stent-graft implantation. AngioCT data from 20 patients aged 50-60 years, before and after stent-graf...
Article
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Up to date, there is no information on the influence of 2,2,2-tribromoethanol (TBE; Avertin), a commonly used anaesthetic, on mice with impaired antioxidant capacity. We aimed to analyse the effect of a single dose of Avertin on anaesthesia duration time, inflammatory response, oxidative stress and collagen deposition in the large intestine of Nrf2...
Article
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Surgical intervention is currently the only option for an abdominal aortic aneurysm (AAA), preventing its rupture and sudden death of a patient. Therefore, it is crucial to determine the pathogenic mechanisms of this disease for the development of effective pharmacological therapies. Oxidative stress is said to be one of the pivotal factors in the...
Article
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The aim of this study was to create a mathematical approach for blood hemodynamic description with the use of brightness analysis. Medical data was collected from three male patients aged from 45 to 65 years with acute type IIIb aortic dissection that started proximal to the left subclavian artery and involved the renal arteries. For the recognitio...
Article
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The scope of tasks of chemical and ecological rescue procedures includes prevention of terrorist attacks with biological weapons. After each action, firefighters are obliged to clean and disinfect their outfits to prevent the potential spreading of harmful microorganisms. This study aimed to analyze the effectiveness of decontamination fluids used...
Article
Endothelial dysfunction accompanied by the loss of endothelial cell phenotype plays an essential role in cardiovascular diseases. Here, we report that inhibition of biliverdin reductase (BVR), the enzyme of the heme degradation pathway converting biliverdin to bilirubin, shifts endothelial phenotype of the primary human aortic endothelial cells (HA...
Article
Full-text available
Premature senescence, a death escaping pathway for cells experiencing stress, is conducive to aging and cardiovascular diseases. The molecular switch between senescent and apoptotic fate remains, however, poorly recognized. Nrf2 is an important transcription factor orchestrating adaptive response to cellular stress. Here, we show that both human pr...
Article
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Objective Neutrophil gelatinase associated lipocalin (NGAL) and matrix metalloproteinase (MMP)-9/NGAL complex were investigated in asymptomatic patients with carotid artery stenosis including gender specific differences aiming at vulnerable plaques prone to embolisation. Methods Serum NGAL and MMP-9/NGAL levels were analysed in 83 patients with as...
Article
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Background In clinical diagnostics, combination of different imaging techniques is applied to assess spatial configuration of the abdominal aortic aneurysm (AAA) and deformation of its wall. As deformation of aneurysm wall is crucial parameter in assessing wall rupture, we aimed to develop and validate a Non-Invasive Vision-Based System (NIVBS) for...
Article
Background and objectives: Post-operative complications of endovascular aneurysm repair (EVAR), such as endoleaks, migration or angular bands in a stent-graft or narrowing and occlusion of a stent-graft lumen are potentially life-threatening and difficult to perceive without constant monitoring. Therefore, our work aimed to propose a new ex-vivo sy...
Article
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Background: We aimed to verify the accuracy of the Computational Fluid Dynamics (CFD) algorithm for blood flow reconstruction for type IIIb aortic dissection (TBAD) before and after thoracic endovascular aortic repair (TEVAR). Methods: We made 3D models of the aorta and its branches using pre- and post-operative CT data from five patients treated f...
Article
Aging is the most critical risk factor for the development of cardiovascular diseases and their complications. Therefore, the fine-tuning of cellular response to getting older is an essential target for prospective therapies in cardiovascular medicine. One of the most promising targets might be the transcription factor Nrf2, which drives the expres...
Conference Paper
This study was undertaken to verify if simvastatin modulates Cav-1/eNOS expression and if this modulation is associated with changes in pro- and anti-inflammatory cytokines and Toll-like receptor 4 (TLR4) in abdominal aortic aneurysm (AAA).
Article
Full-text available
Heme oxygenase-1 (HO-1), encoded by HMOX1 gene and regulated by Nrf2 transcription factor, is a cytoprotective enzyme. Its deficiency may exacerbate abdominal aortic aneurysm (AAA) development, which is also often associated with hyperlipidemia. Beneficial effects of statins, the broadly used antilipidemic drugs, were attributed to modulation of Nr...
Article
Full-text available
This study was undertaken to verify whether simvastatin modulates Cav-1/eNOS expression, and if this modulation is associated with changes in pro- and anti-inflammatory cytokine and Toll-like receptor 4 (TLR4) level in abdominal aortic aneurysm (AAA). It is a 1:2 case-control study of non-statin (n=12) and simvastatin-treated patients (n=24) who un...
Article
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Complexity of the spatial configuration of an aortic implant with bifurcation in the distal part is related to changes in blood hemodynamic in the area of bifurcation which may disturb blood flow and lead to thrombus formation. This study was designed to characterize parameters which define spatial configuration of an aortic implant for which the r...
Article
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Background: Neutrophil gelatinase-associated lipocalin (NGAL), an acute phase protein released by neutrophils, has been described as biomarker of inflammatory states. Type 2 diabetes mellitus (T2DM) is characterized by increased inflammation and an elevated risk for embolization of carotid artery stenosis (CAS). We aimed to explore the role of NGA...
Article
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Background Cyclophilin A (CyPA) is an immunomodulatory protein, high expression of which correlates with poor outcome of patients with inflammatory diseases. However, its role in inflammatory bowel disease (IBD) has not been studied. AimThis study analyzes the correlation between cyclophilin A, matrix metalloproteinase (MMP)-9, and tissue inhibitor...
Article
Aim: To investigate the levels of G protein-coupled receptor 55 (GPR55) expression in colonic tissue of inflammatory bowel disease (IBD) patients and healthy controls, and its potential implication in IBD treatment. Methods: Fifty patients were enrolled in our prospective study: n = 21 with Crohn's disease (CD) and n = 16 with ulcerative colitis...
Article
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Purpose: Periostin is considered a biomarker for eosinophilic airway inflammation and have been associated with NSAID-Exacerbated Respiratory Disease (NERD) and chronic rhinosinusitis (CRS). In this study, we aimed to evaluate periostin in exhaled breath condensate (EBC) and in serum of patients with various asthma phenotypes. Methods: The study...
Article
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Background & Aims: G protein-coupled receptor 30 (GPR30) is a recently de-orphanized estrogen receptor that mediates the effects of estrogens on different cells. It has been postulated that in inflammatory bowel diseases (IBD) activation of GPR30 blocks the pathways dependent on pro-inflammatory cytokines. The aim of our study was to investigate GP...
Article
Aim: This study was undertaken to verify if two-weeks treatment of lipoic acid (LA) influence colon damage and pro-inflammatory cytokine synthesis during DSS-induced acute colitis. Moreover, as LA has anti-oxidative properties, we analyzed its influence on the level of antioxidative enzymes, HO-1 and eNOS, and their regulator- caveolin-1. Methods:...
Article
The aim of this study was to assess the immune response to parainfluenza virus type 3 (PIV3), rhinovirus 1B (RV1B) and intracellular Toll-like receptors (TLR) agonists in nasal epithelial cells (NECs) from patients with allergic rhinitis and healthy controls. NECs were obtained from eight patients with allergic rhinitis (AR) and 11 non-atopic healt...
Article
Cardiovascular diseases (CVD) are associated with dysfunction of endothelium which regulates vascular wall contractile state and cellular composition. Latest large clinical trials indicated that lipid-modifying interventions decrease the risk of CVD in patients with hypercholesterolemia and in those with relatively normal levels of LDL cholesterol....
Article
Objective: Neutrophil gelatinase-associated lipocalin (NGAL), a protein found in activated neutrophils, is expressed in kidney tubule cells in response to noxious stimuli, and is thus recognized as a marker of acute kidney injury. Recent studies have suggested that NGAL could also have pathophysiological importance in cardiovascular diseases. The...
Article
Introduction. Model of primary bronchial epithelial cells culture has been used to study the innate response to viral infections in patients with bronchial asthma. Aim. The aim of the study was to evaluate the effectiveness of bronchial epithelial cell culture and the influence of parainfluenza virus type 3 (PIV- 3) on cell morphology. Materials an...
Article
The α7 pentamer nicotinic acetylcholine receptors (nAChRs) are a target in transduction of anti-inflammatory signals from the central nervous system (CNS) to the gastrointestinal (GI) tract. The aim of this study was to investigate the anti-inflammatory action of the novel α7 nAChR partial agonist, encenicline and to determine the mechanism underly...
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The pathogenesis of the abdominal aortic aneurysm (AAA) shows several hallmarks of atherosclerotic and atherothrombotic disease, but comprises an additional, predominant feature of proteolysis resulting in the degradation and destabilization of the aortic wall. This review aims to summarize the current knowledge on AAA development, involving the ac...
Article
Matrix metalloproteinases (MMPs) play a pivotal role in the development and progression of abdominal aortic aneurysms (AAAs). The action of MMPs depends on a balance between tissue inhibitors of MMPs (TIMPs) and compounds that may prolong protease activity, such as neutrophil gelatinase-associated lipocalin (NGAL). The study was designed to analyse...
Article
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Epidemiological data suggest that the consumption of polyphenol-rich foods reduces the incidence of cancer, coronary heart disease, and inflammation. Chlorogenic acid (CGA), an ester of caffeic and quinic acids, is one of the most abundant polyphenol compounds in human diet with proven biological effectiveness both in vitro and in vivo. The aim of...
Article
Celiac disease (CD) is an autoimmune disease induced by an autoimmune reaction to indigested gluten, which occurs in genetically predisposed population. The etiology of CD is linked to innate and adaptive immunity, mostly mediated by lymphocytes, especially T cells, infiltrating into the small intestinal wall. The subpopulations of T cells that inf...
Article
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Oenothera paradoxa (EP) preparations are commonly used in folk medicine to treat skin diseases, neuralgia, and gastrointestinal (GI) disorders. Several reports suggested that EP preparations exhibit potent anti-inflammatory and antioxidant activities both in vitro and in vivo. Here, we aimed to characterize the action of EP pomace polyphenol extrac...
Article
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In this review, we focus on the role of oxidative stress in the aetiology of inflammatory bowel diseases (IBD) and colitis-associated colorectal cancer and discuss free radicals and free radical-stimulated pathways as pharmacological targets for anti-IBD drugs. We also suggest novel anti-oxidative agents, which may become effective and less-toxic a...
Article
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Natural and synthetic derivatives of benzo-γ-pyrones (i.e. flavones, chromones, and coumarins) and their synthetic analogues possess a wide range of biological properties in vitro and in vivo. In this paper we investigated the influence of two hydrazone compounds of chromones, 3-{[(2-dimethoxytiophosphoryl)-2-methylhydrazono]-methyl}-chromen-4-one...
Article
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Background: Bacterial endotoxin (lipopolysaccharide - LPS) is a strong modulator of the immune system that plays a crucial role in the pathogenesis of endotoxic shock. The aim of the study was to investigate the effects of lipoic acid (LA) on oxidative stress markers in spleen homogenates obtained from LPS-induced endotoxic shock rats. Methods:...
Article
BACKGROUND: Cyclophilin A (CyPA), a cyclosporine A-binding protein, influences abdominal aortic aneurysm (AAA) formation and the ERK1/2 signalling pathway in animal and in vitro studies. Statins decrease CyPA in smooth muscle cells although their influence on CyPA in human AAA is unknown. MATERIAL AND METHODS: The study was performed on AAA wall-ti...
Article
was to evaluate the influence of nuclear factor-kappaB inhibition on -SH groups concentration in liver of rat Experiments were performed on Wistar-Kyoto rats divided into following groups: group I (control) received saline; group II (ET-1) received endothelin 1 (12.5 microg/kg b.w.); Group III (BAY 11-7082) received a nuclear factor-kappaB (NF-kapp...