Aleksandra Kładna

• Pomeranian Medical University

Background: A series of eight new flavone derivatives containing a piperazine chain with different substitution were synthesized and their structures were determined. Methods: Their antiradical and antioxidant activities were evaluated using superoxide anion radical, hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl radical, 2,2'-azino-di(3-ethylb...

Background Stilbene phytalexis (1,2-diphenyloethylen) and benzamide are beneficial for human health. To increase the stilbene ring activity, a new series of its derivatives containing benzamide structure was synthesized and evaluated for their in vitro antioxidant power. Methods 1H nuclear magnetic resonance, mass spectroscopy, and chromatographic...

The body of evidence from the past three decades demonstrates that oxidative stress can be involved in several diseases. This study aims to summarise the current state of knowledge on the association between oxidative stress and the pathogenesis of some characteristic to the biological systems diseases and aging process. This review also presents t...

So far, no article concerning the history of epidemiological studies on myopia in Poland after World War II has been published. Therefore, the aim of the work is to present the history of epidemiological studies on myopia in Poland after World War II. In order to obtain answers to the research questions, studies of source and archival materials wer...

Aim: Synthesis of novel 2(2-hydroxyphenyl) pyrimidine/benzothiazole piperazinyl-substituted flavones end evaluate their antioxidant activity. Results: Six novel 2-(2-hydroxyphenyl) pyrimidine/benzothiazole-substituted flavones were synthesized, their structures were confirmed by elementary and spectral analyses. The compounds were evaluated for...

In the conducted examinations we determined that the leading center in myopia research after World War II in Poland is the Pomeranian Medical Academy (Pomeranian Medical University). In Szczecin after the World War II, various areas of ophthalmology developed significantly. Particularly intense development occurred in the field of myopia. In the co...

Background: A new series of 13 piperazinyl flavone derivatives has been synthesized and examined for their in vitro antiradical and antioxidant activities as response to the pharmacy industry's increasing demand for new non-toxic anti-inflammatory and anticancer drugs. Methods: Their antioxidant activity was evaluated by the reactive oxygen spec...

Flavones exhibit a variety of beneficial effects and are well known for their medicinal importance in several diseases, including cardiovascular, neurodegenerative and cancer. The inclusion of the piperazine ring to the flavone backbone is an important strategy in drug discovery but only a few studies have synthesized piperazinyl flavone compounds...

The aim of this study was to characterize the antioxidant activity of penicillin G (PG), ampicillin (AMP), oxacillin (OX) and dicloxacillin (DOX) through their reactivity towards reactive oxygen species (superoxide anion radical, ; hydroxyl radical, HO•; peroxyl radical, ROO•; hydrogen peroxide, H2O2; DPPH•) using various in vitro antioxidant assay...

Isoflavones genistein and daidzein are nonsteroidal phytoestrogens occurring mainly in soybean foods. These phytoestrogens possess estrogenic properties and show a variety of health benefits as anti-inflammatory agents. However, the mechanism of their action has not been identified in detail. The aim of this study is to characterize the antioxidant...

Pyruvic acid and its derivatives occurring in most biological systems are known to exhibit several pharmacological properties, such as anti-inflammatory, neuroprotective or anticancer, many of which are suggested to originate from their antioxidant and free radical scavenger activity. The therapeutic potential of these compounds is a matter of part...

A series of flavonyl-2,4-thiazolidinedione, imidazolidinedione and rhodanine derivatives were tested for their antioxidant activity as scavengers of oxygen free radicals. Free radical scavenging activities, including superoxide anion radical , hydroxyl radical (HO•) and 2,2′-diphenyl-1-picrylhydrazyl free radical have been evaluated using chemilumi...

Recent reviews evidence that the naturally occurring compounds containing the chromone skeleton exhibit antiradical activities, providing protection against oxidative stress. The antioxidant activities of 13 new synthesized chromonyl-2,4-thiazolidinediones, chromonyl-2,4-imidazolidinediones and chromonyl-2-thioxoimidzolidine-4-ones were evaluated u...

Fifteen chromonylrhodamine derivatives (CRs) were synthesized and the antioxidant activity levels were evaluated for the first time. The antioxidant activity potencies of these chromone derivatives were evaluated towards superoxide anion radicals, hydroxyl radicals and 2,2-diphenyl-1-picrylhydrazyl radicals. Also, the total antioxidant capacity of...

The antioxidant properties of 11 new synthesized chromonyl-2,4-thiazolidinediones and chromonyl-2,4-imidazolidinediones (CBs) were investigated. The antioxidant activities and mechanisms of the CBs interaction with reactive oxygen species (ROS) were clarified using various in vitro antioxidant assay methods including superoxide anion radical ([Form...

The direct effect of the four catecholamines (adrenaline, noradrenaline, dopamine and isoproterenol) on superoxide anion radicals (O2-•) was investigated. The reaction between 18-crown-6-ether and potassium superoxide in dimethylsulfoxide was used as a source of O2-•. The reactivity of catecholamines with O2-• was examined using chemiluminescence,...

The direct effects of the four catecholamines (CATs), adrenaline (A), noradrenaline (NA), dopamine (D) and isoproterenol (I), on free radicals were investigated using the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH(•) ) and hydroxyl radial (HO(•) ). The CATs examined were found to inhibit the ESR signal intensity of DPPH(•) in a dose-dependent...

Tetracyclines are the second most common antibiotic family in medicine usage. These antibiotics exhibit antioxidant potential; however, the exact mechanism remains unclear. The antiradical activity of the seven tetracyclines (TCs; tetracycline, chlortetracycline, oxytetracycline, doxocycline, methacycline, demeclocycline, minocycline) was determine...

Tetracyclines are the second most common antibiotic family in the human and veterinary medicine usage, worldwide. These groups of drugs originally developed as antibiotics with antibacterial activity are able to exert biological effects different from their antimicrobial action. The actions occur by multiple mechanisms and include inflammatory cyto...

Carazolol [4-(2-hydroxy-3-isopropyl-amino-propoxy)-carbazole], a β(3)-adrenoceptor agonist, is clinically used in the treatment of hypertension, cardiac arrhythmias and angina pectoris. Despite the beneficial effect of the drug, its high dose may contribute to cardiotoxicity. This study was conducted to examine whether carazolol can influence hydro...

A chemiluminescent technique was applied to determine antioxidative activities of adriamycin, farmorubicin, mitomycin C and bleomycin against superoxide anion radical (O(2)(•)) in aprotic medium. The antioxidant capacity was expressed as the decrease in light emission from the O(2)(•) solution by and antibiotic. A KO(2) solution in dimethyl sulphox...

The tetracycline family antibiotics are widely used as human and veterinary treatments. The drugs are effective as antibiotics and also show antimicrobial and non-microbial action. However, the antioxidant properties of tetracyclines have not been characterized in aprotic media. To better understand their biological functions, the in vitro superoxi...

The purpose of this study was to identify the antioxidant activity of 16 compounds isolated from Piper cubeba (CNCs) through the extent of their capacities to scavenge free radicals, hydroxyl radical (HO(•)), superoxide anion radical O•(2)(-) and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH(•)), in different systems. Electron paramagnetic resonance...

Free radical scavenging activity of flavonyl-thiazolidine-2,4-dione compounds has been evaluated using chemiluminescence, electron spin resonance spectroscopy with 5,5-dimethyl-1-pyrroline-1-oxide as spin trap and DPPH (2,2'-diphenyl-1-picrylhydrazyl) method. The examined compounds exhibited 28-50% scavenging superoxide anion radical O₂⁻ⁱ16.7-76.7%...

Communication and narrative skills development is considered as an essential prerequisite to acquire and exhibit empathic behavior in clinical context. Students' capacity for empathy can be also enhanced by their ability to control own emotion and stress level. Thus, educators have developed and implemented educational programs to improve students'...

Literature, theatrical performances, films and the arts could be used to enhance empathy among students of medicine and residents. The use of these media enables them to learn how feelings are expressed. Through study of literature and the performing arts students can also gain new insights into their own emotional responses to illness and sufferin...

Clinical empathy is a multidimensional concept consisting of four attributes: cognitive, emotional, behavioral, and motivational. Empathy involves an interest in and understanding of patients' experiences, concerns, and perspectives associated with illness combined with a capacity to care and an intention to help. Empathy in the context of clinical...

Medical training should aim at providing students' with opportunities to learn about and explore their cognitive and emotional responses to patients' illness and suffering. The training should also foster understanding of a patient's experiences and encourage empathic communication. Teaching approaches that use experiential learning methods seem to...

The antioxidant behavior of a series of new synthesized substituted thiazolyl-thiazolidine-2,4-dione compounds (TZDs) was examined using chemiluminescence and electron paramagnetic resonance spin trapping techniques. 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was used as the spin trap. The reactivity of TZDs with superoxide anion radical (O2(*-)) and...

Phenolic compounds are widely present in plants and they have received considerable attention due to their antioxidant property. In this article we report the results of a study of the reactivity of 10 selected phenolics (sesamol, three phenolic acids, three flavonols, one flavone, and two flavanones) with superoxide anion radical (O ), hydroxyl ra...

Ketoprofen and tolmetin are widely used nonsteroidal anti-inflammatory drugs, whereas fenofibrate belongs to a family of hypolipidemic drugs used in the prevention of cardiovascular diseases. The aim of this study was to assess effect of these drugs on reactions generating reactive oxygen species (ROS). The following generators of ROS were used: 18...

Background. Diseases occurring in old age cause many health and social problems related to handicap. Particularly vulnerable to these problems are lonely living old patients. Material and methods. The study was a descriptive one and concerned a group of 135 patients aged 65 or more, living lonely. Data were obtained from medical records and owing t...

Bucillamine (BUC) is used clinically for the treatment of rheumatoid arthritis. Some of the pharmacological action of BUC has been reported as being dependent on the production of reactive oxygen species (ROS). In this paper the reactivity of BUC with superoxide anion radical (O2•−) generated from potassium superoxide/18-crown-6 ether dissolved in...

The antioxidant behavior of a series of new synthesized substituted indoline-2-ones and indolin-2-thiones was investigated in this study using an oxygen radical absorbance capacity assay (ORAC(ROO*-) and 2,2'-azobis(2-amidino-propane) dihydrochloride (AAPH) as the radical generator; system generating superoxide anion radical, O2*- (18-crown-6/KO(2)...

The plant-derived phenolic compounds genistein and oleuropein are known to exhibit several biological properties, many of which may result from their antioxidant and free radical scavenger activity. In this paper we report the results of a complex study of antioxidant activity of genistein and oleuropein, using electron spin resonance (ESR), chemil...

4-(4-Phenoxybenzoyl)benzoic acid derivatives (PBADs) were found to inhibit rat and human alpha-reductase isozymes 1 and 2 in vitro. Chemiluminescence (CL), electron spin resonance, spin trapping techniques, and spectrophotometry were used to examine the effect of PBADs on reactive oxygen species (superoxide radical, O(2)(.-); hydroxyl radical, HO(*...

Indole-2 and 3-carboxamides (IDs) are proposed to be selective cyclooxygenase inhibitors. Since cyclooxygenase-1 may be involved in reactive oxygen species (ROS) production, we hypothesize that these indole derivatives have antioxidative properties. We have employed chemiluminescence (CL) and electron spin resonance (ESR) spin trapping to examine t...

The oxidation of melatonin (MEL) using the Cu(II) + H(2)O(2) + HO(-) (the Fenton-like reaction) system was investigated by chemiluminescence (CL), fluorescence, spectrophotometric, and EPR spin trapping techniques. The reaction exhibits CL in the 400-730 nm region. The light emission from the Fenton-like reaction was greatly enhanced in the presenc...

Psoralens (psoralen, 5-methoxypsoralen, 8-methoxypsoralen, khellin, and visnagin) in 1 mM doses were shown to enhance the generation of reactive oxygen species, such as the hydroxyl radical (HO*), the superoxide anion radical (O2(-)), and singlet oxygen ((1)O(2)), from the system generating chemiluminescence (CL), as well as free radicals in the ab...

The generation of singlet molecular oxygen ((1)O(2)) and hydroxyl radicals (HO*) during peroxidation of bopindolol in the presence of Co(II) ions was studied using electron spin resonance (ESR) and spectrophotometry methods. 2,2,6,6-Tetramethyl-4-piperidone and 5,5-dimethyl-1-pyrroline-1-oxide were used as traps. The spectrophotometry determination...

Despite beneficial effect of isoproterenol and doputamine, synthetic catecholamines rutinely used in intensive care patients, their high doses may contribute to cardiotoxicity. This study was conducted to examine whether these drugs can influence on Co, Cu - catalyzed generation deleterious reactive oxygen species (ROS) from H 2 O 2 using chemilumi...

The enhanced generation of singlet oxygen (1O2) during oxidation of farmorubicin in the Co(II) + H2O2 system was studied using chemiluminescent, fluorescent and spectrophotometic techniques. The influence of 1O2-quenchers, catalase, superoxide anion radical (O2*-) and hydroxyl radical (HO*) scavengers on the light emission was studied. The spectrop...

The effect of 4-flavonil-1,4-dihydropyridine derivatives on a chemical system involving a superoxide radical anion was tested using the chemiluminescence and spectrophotometry methods. All tested compounds enhanced the light emission from the system. The obtained results indicated that the tested derivatives may catalyze the conversion of superoxid...

The effects of thymol (TOH), thymoquinone (TQ) and dithymoquinone (TQ2) on the reactions generating reactive oxygen species (ROS) such as superoxide anion radical (O−2), hydroxyl radical (HO) and singlet oxygen () were tested using the chemiluminescence (CL) and spectrophotometry methods. All tested compounds acted as scavengers of various ROS. The...

Chemiluminescence appearing during oxidation of isoproterenol using chemical system involving superoxide anion radical has been studied. Chemiluminescence and fluorescence spectra were measured. The chemiluminescence spectrum was measured with cut‐off filters and revealed bands with maximum at 440, 480, 550, 640 and 700 nm. The bands at 480, 640 an...

The aim of the present study was to investigate the inhibitory effect of a number of biologically important compounds on catecholamines (adrenaline, noradrenaline, dopamine) peroxidation with respect to production of reactive oxygen species, especially hydroxyl radicals. Measurements were done using a chemiluminescence technique, in presence of the...