Aleksandra Bednarz

Aleksandra Bednarz
Jagiellonian University | UJ · Faculty of Biochemistry, Biophysics and Biotechnology

PhD

About

23
Publications
3,293
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126
Citations

Publications

Publications (23)
Article
Full-text available
The demand for iron is high in pregnancy to meet the increased requirements for erythropoiesis. Even pregnant females with initially iron‐replete stores develop iron‐deficiency anemia, due to inadequate iron absorption. In anemic females, the maternal iron supply is dedicated to maintaining iron metabolism in the fetus and placenta. Here, using a m...
Article
Full-text available
Owing to its redox properties, copper is a cofactor of enzymes that catalyze reactions in fundamental metabolic processes. However, copper–oxygen interaction, which is a source of toxic oxygen radicals generated by the Fenton reaction, makes copper a doubled-edged-sword in an oxygen environment. Among the microelements influencing male fertility, c...
Article
Full-text available
In most mammals, neonatal intravascular hemolysis is a benign and moderate disorder that usually does not lead to anemia. During the neonatal period, kidneys play a key role in detoxification and recirculation of iron species released from red blood cells (RBC) and filtered out by glomeruli to the primary urine. Activity of heme oxygenase 1 (HO1),...
Article
Full-text available
Iron deficiency is the most common mammalian nutritional deficiency during the neonatal period. However, among mammalian species neonatal iron deficiency anemia (IDA), the most severe consequence of iron scarcity, occurs regularly in pigs. Although intramuscular supplementation of piglets with high amounts of iron dextran (FeDex) is largely conside...
Article
Full-text available
Neutrophil extracellular traps (NETs) contribute to pathological disorders, and their release was directly linked to numerous diseases. With intravital microscopy (IVM), we showed previously that NETs also contribute to the pathology of systemic inflammation and are strongly deposited in liver sinusoids. Over a decade since NET discovery, still not...
Article
Full-text available
Moderate intravascular hemolysis is a common condition in newborns. It is followed by the accumulation of bilirubin, which is a secondary product of the activity of heme oxygenase-1, an enzyme that catalyzes the breakdown of heme released from disrupted erythrocytes and taken up by hepatic macrophages. Although these cells are a major site of enzym...
Article
Jackson toxic milk mutant mice (tx-J) carrying a missense mutation in the Atp7b gene are animal models of the Wilson disease. In both the Wilson patients and the tx-J mice, mutations in the ATP7B/Atp7b gene lead to disturbances in copper metabolism. The dysfunction of ATP7B/Atp7b leads to a reduction in the incorporation of copper into apocerulopla...
Article
Full-text available
Progressive functional maturation of spermatozoa is completed during the transit of these cells through the epididymis, a tubule structure connecting a testicle to a vas deferens. Epididymal epithelial cells by means of their secretory and absorptive functions determine a highly specialized luminal microenvironment containing multiple organic and i...
Article
The maintenance of copper homeostasis is critical for all cells. As learned from mice with disturbed copper metabolism, this trace element is also important for spermatogenesis. The experiments conducted in yeasts have demonstrated that appropriate copper level must be preserved to enable meiosis progression; however, increased copper level is toxi...
Article
Full-text available
Heme is an efficient source of iron in the diet, and heme preparations are used to prevent and cure iron deficiency anemia in humans and animals. However, the molecular mechanisms responsible for heme absorption remain only partially characterized. Here, we employed young iron-deficient piglets as a convenient animal model to determine the efficacy...
Data
Mean body weight gain in piglets from the 1st to the 28th day after birth (mean ± S.D.). (DOCX)
Data
Validation of the cross-reactivity of antibodies with pig proteins analyzed in the study. Comparative Western blot analysis shows that primary antibodies cross-react with mouse as well as with pig proteins. For the analysis crude membrane and cytosolic protein extracts (40–60 μg protein) were prepared from duodenal scrapings as described previously...
Data
Mean daily feed and iron intake by piglets during 4 main periods after birth. (DOCX)
Data
List of oligonucleotide primers used for RT-qPCR. (DOCX)
Data
Blastn and blastp analysis for examined genes and proteins. (DOCX)
Data
List of antibodies used for Western blot analyses. (DOCX)
Conference Paper
Objectives: Wilson disease (WD) is an autosomal disorder of copper metabolism caused by mutations in the ATP7B gene and leading to the copper accumulation in the liver, brain and other organs. Case reports and animal studies suggest that alteration in iron metabolism can be connected with WD. The aim of this study was to investigate iron metabolism...
Article
Mosaic mutant mice displaying functional dysfunction of Atp7a copper transporter (the Menkes ATPase) are an established animal model of Menkes disease and constitute a convenient tool for investigating connections between copper and iron metabolisms. This model allows to explore changes in iron metabolism in suckling mutant mice suffering from syst...
Conference Paper
Ferroportin (Fpn) is the only known mammalian non-heme iron exporter expressed at the membrane of duodenal enterocytes and macrophages of the spleen and the liver. It plays a crucial role in transporting iron from the duodenum to the serum and iron recycling from senescent red blood cells back into the circulation, respectively. Fpn expression is r...
Article
Full-text available
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterized by degeneration and loss of motor neurons in the spinal cord, brainstem and motor cortex. Up to 10% of ALS cases are inherited (familial, fALS) and associated with mutations, frequently in the superoxide dismutase 1 (SOD1) gene. Rodent transgenic models of...
Article
Full-text available
Among livestock, domestic pig (Sus scrofa) is a species, in which iron metabolism has been most intensively examined during last decade. The obvious reason for studying the regulation of iron homeostasis especially in young pigs is neonatal iron deficiency anemia commonly occurring in these animals. Moreover, supplementation of essentially all comm...
Article
Full-text available
The biological interaction between copper and iron is best exemplified by the decreased activity of multicopper ferroxidases under conditions of copper deficiency that limits the availability of iron for erythropoiesis. However, little is known about how copper deficiency affects iron homeostasis through alteration of the activity of other copper-c...

Questions

Question (1)
Question
I need to perform a seperate IF staning on kidney sections of marker protein for proximal tubules and for tested protein. Unfortunately, I can't make double staining on the same tissue section because of lack of appropriate antibodies 
(I have to use primary antibodies produced in the same host). That's why I'm considering IF on serial sections. What is the best method for this kind of staining?

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