Alejandro B Rodriguez-Navarro

Alejandro B Rodriguez-Navarro
University of Granada | UGR · Department of Mineralogy and Petrology

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185
Publications
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Publications

Publications (185)
Article
The effect of Hg and PCBs (Aroclor 1268) on bone characteristics was investigated in a population of Clapper Rails (Rallus longirostris) inhabiting contaminated and unimpacted estuarine marsh systems in coastal Georgia. Exposure to contaminants did not affect the length or weight of leg bones, but it significantly altered the chemical composition o...
Article
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For a fuller understanding of the functionality of the eggshell cuticle, we conducted a detailed study using a wide array of analytical techniques (scanning and transmission microscopy), energy dispersive x-rays, and attenuated total reflection-Fourier transform infrared spectroscopy to analyze the structure, morphology, and chemical composition of...
Data
Future ocean acidification (OA) will affect physiological traits of marine species, with calcifying species being particularly vulnerable. As OA entails high energy demands, particularly during the rapid juvenile growth phase, food supply may play a key role in the response of marine organisms to OA. We experimentally evaluated the role of food sup...
Article
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The external surface microornament of the glass scallops Catillopecten natalyae and malyutinae is made by calcitic spiny projections consisting of a stem that later divides into three equally spaced and inclined branches (here called aerials). C. natalyae contains larger and smaller aerials, whereas C. malyutinae only secreted aerials of the second...
Article
The importance of egg natural defences to prevent bacterial contamination and their relation with hen age in extended production cycles were evaluated. Egg-white from eggs of different hen age groups (up 100-weeks-old) and lines (Hy-Line white and brown) were inoculated with Gram-positive Staphylococcus aureus or Gram-negative Salmonella Typhimuriu...
Article
Environmental variation alters biological interactions and their ecological and evolutionary consequences. In coastal systems, trematode parasites affect their hosts by disrupting their life-history traits. However, the effects of parasitism could be variable and dependent on the prevailing environmental conditions where the host-parasite interacti...
Article
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Keel bone fractures are a serious animal welfare problem in laying hens. The aim of the current study was to assess the influence of egg production, estradiol-17ß, and selection for high laying performance on bone quality. Hens of two layer lines differing in laying performance (WLA: 320 eggs per year, G11: 200 eggs per year) were allocated to four...
Article
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Cleidoic eggs possess very efficient and orchestrated systems to protect the embryo from external microbes until hatch. The cuticle is a proteinaceous layer on the shell surface in many bird and some reptile species. An intact cuticle forms a pore plug to occlude respiratory pores and is an effective physical and chemical barrier against microbial...
Chapter
The avian egg is a giant reproductive cell protected by an eggshell, which biomineralizes in the uterus, in an acellular fluid that contains ionic and organic precursors. The distinctive features of the eggshell, as compared to bone or teeth, are its mineral composition (CaCO3 in the form of calcite) and the absence of cell-directed assembly during...
Article
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The exposure to environmental variations in pH and temperature has proven impacts on benthic ectotherms calcifiers, as evidenced by tradeoffs between physiological processes. However, how these stressors affect structure and functionality of mollusk shells has received less attention. Episodic events of upwelling of deep cold and low pH waters are...
Article
Purpose: To investigate the effects of four commercial silver diamine fluoride (SDF) agents on the chemical composition and microstructural properties of dentin, and its relation to the bond strength of two adhesives. Materials and methods: Ninety human molars were randomly divided into sound dentin (negative control), demineralized dentin (posi...
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Background The physiological adaptations that have evolved for egg laying make hens susceptible to bone fractures and keel bone damage. In modern laying hen breeds, longer periods of egg laying could result in a greater risk of poor bone quality, and selection for increased egg production has frequently been stated to be a cause. However, the exist...
Article
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The bacterial load on the eggshell surface is a key factor in predicting the bacterial penetration and contamination of the egg interior. The eggshell cuticle is the first line of defense against vertical penetration by microbial food-borne pathogens such as Salmonella Enteritidis. Egg producers are increasingly introducing alternative caging syste...
Article
The shell of the cephalopod Argonauta consists of two layers of fibers that elongate perpendicular to the shell surfaces. Fibers have a high-Mg calcitic core sheathed by thin organic membranes (>100 nm), and configurate a polygonal network in cross-section. Their evolution has been studied by serial sectioning with electron microscopy-associated te...
Article
Objective: This study evaluated the potential combined effects of nanohydroxyapatite and proanthocyanidin on the remineralization and collagen stabilization of demineralized dentin. Methods: Seventy-five coronal dentin beams (6 × 1 × 1 mm3) were randomly allocated into five experimental groups (n = 15): Sound (no treatment), Control (pH-cycling)...
Article
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After thermal modification at 210 °C using the Thermowood method, changes in Pinus radiata D. Don wood cell wall components and hygroscopicity were studied for subsequent comparison with recently felled wood of the same species in samples from the same region of provenance (Basque Country, Spain). To this end, samples were characterised by sorption...
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Laternula elliptica is a key bivalve species and widely distributed around the Antarctic continent. This bivalve has been the study subject in several studies centered on ecological, physiological, biochemical, and behavioral patterns. However, little is known about the chemistry and the biomechanical properties of the shells of this mollusk. Here,...
Preprint
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Osteoporosis and bone fractures are a severe problem for the welfare of laying hens, with genetics and environment, such as housing system, each making substantial contributions to bone strength. In this work, we performed genetic analyses of bone strength, bone mineral density and bone composition, as well as body weight, in 860 commercial crossbr...
Article
• Bone properties are adapted to their specific functions in the animal, so various types of bones develop different characteristics depending on their location in the skeleton. • The aim of this research was to compare the chemical composition, crystalline characteristics and structural organisation in tibiotarsus, humerus and keel bones as repres...
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The objective of the present study was to investigate the effects of chronic lead exposure on the mineral properties of alveolar bone. For this purpose, female Wistar rats (n = 8) were exposed to 1000 ppm lead acetate in drinking water for 90 days, while the control group (n = 5) was treated with sodium acetate. The alveolar bone structure and chem...
Article
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We have studied in detail the changes that occur in eggshell structure and composition during a production cycle in order to better understand the deterioration of eggshell quality with hen age (at 33, 45, and 67 weeks). To study changes in eggshell ultrastructure and microstructure characteristics (mammillary density, palisade layer thickness, siz...
Article
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Objectives This study compared the chemical composition, microstructural, and mechanical properties of human and bovine dentin subjected to a demineralization/remineralization process.Materials and methodsHuman and bovine incisors were sectioned to obtain 120 coronal dentin beams (6 × 1 × 1 mm3) that were randomly allocated into 4 subgroups (n = 15...
Article
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(1) Background: Nowadays the industry aims to improve lay persistency for extended cycles (100 weeks or longer) to make egg production more sustainable. However, intensive egg production challenges hen health, inducing severe osteoporosis and the incidence of bone fractures. In this study, the relationship between bone quality and egg production, a...
Article
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An amendment to this paper has been published and can be accessed via the original article.
Article
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Background The avian eggshell is a natural protective envelope that relies on the phenomenon of biomineralization for its formation. The shell is made of calcium carbonate in the form of calcite, which contains hundreds of proteins that interact with the mineral phase controlling its formation and structural organization, and thus determine the mec...
Article
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Background: Skeletal damage is a challenge for laying hens because the physiological adaptations required for egg laying make them susceptible to osteoporosis. Previously, we showed that genetic factors explain 40% of the variation in end of lay bone quality and we detected a quantitative trait locus (QTL) of large effect on chicken chromosome 1....
Article
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The calcite grains forming the wall plates of the giant barnacle Austramegabalanus psittacus have a distinctive surface roughness made of variously sized crystalline nanoprotrusions covered by extremely thin amorphous pellicles. This biphase (crystalline-amorphous) structure also penetrates through the crystal’s interiors, forming a web-like struct...
Article
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Amorphous calcium carbonate (ACC) is an unstable mineral phase, which is progressively transformed into aragonite or calcite in biomineralization of marine invertebrate shells or avian eggshells, respectively. We have previously proposed a model of vesicular transport to provide stabilized ACC in chicken uterine fluid where eggshell mineralization...
Article
Environmental variation may alter biological interactions and their ecological consequences. For instance, in marine ecosystems hosts and parasites are subject to environmental variability across latitudinal gradients, and their co-evolutionary dynamics may be the result of the interplay with local physical-chemical variables in seawater. Thus, ass...
Article
Understanding how marine species cope with the natural environmental variability of their native habitats will provide significant information about their sensitivity to the potential environmental changes driven by climate change. In particular, marine species inhabiting upwelling ecosystems are experiencing low seawater temperatures, as well as,...
Article
Full-text available
The hygroscopicity and thermodynamic properties of Pinus sylvestris L. wood from a coffin allegedly holding the remains of famous Spanish author Miguel de Cervantes Saavedra (1547-1616) were studied using the 15 • C and 35 • C isotherms fitted to the Guggenheim-Anderson-de Boer model and comparing them with the isotherms of recently felled wood of...
Article
Hydrogels present model systems for biopolymer matrices in biological structural materials, as their fabric and physicochemical properties can be tailored to mimic characteristics of polymer matrices in biological hard tissues. However, hydrogels comprise synthetic compounds and lack attributes of native biopolymers, in contrast to extracellular po...
Preprint
Full-text available
Amorphous calcium carbonate (ACC) is an unstable mineral phase, which is progressively transformed into aragonite or calcite in biomineralization of marine invertebrate shells or avian eggshells, respectively. We have previously proposed a model of vesicular transport to provide stabilized ACC in chicken uterine fluid where mineralization takes pla...
Article
Coastal ecosystems influenced by river discharges are subjected to important environmental changes. Understanding how marine biota cope with its environment is relevant in predicting the responses to future conditions imposed by climate change. To date, a large number of studies have addressed the role of pH on shell and biomineralization propertie...
Article
Mammalian otoconia of the inner ear vestibular apparatus are calcium carbonate-containing mineralized structures critical in maintaining balance and detecting linear acceleration. The mineral phase of otoconia is calcite, which coherently diffracts X-rays much like a single-crystal. Otoconia contain osteopontin (OPN), a mineral-binding protein infl...
Article
In biomineralization, it is essential to know the microstructural and crystallographic organization of natural hard tissues. This knowledge is virtually absent in the case of barnacles. Here, we have examined the crystal morphology and orientation of the wall plates of the giant barnacle Austromegabalanus psittacus by means of optical and electron...
Article
An increasing concern for natural resources preservation and environmental safety is the removal of heavy metals from contaminated water. It is essential to develop simple procedures that use ecofriendly materials with high removal capacities. In this context, we have synthesized a new hybrid material in which eggshell membranes (ESMs) act as nucle...
Article
Mineralized structures generated under biological control are hierarchical composites that consist of biopolymer matrices and minerals. The biopolymer matrix in biological material is developed as membranes or as a network of fibrils, which has structural as well as functional roles for the composite hard tissue. Microorganisms are surrounded by mi...
Article
Arctic calcifiers are believed to be particularly vulnerable to ocean acidification as the Arctic already experiences low carbonate saturations states due to low temperature and high inputs of freshwater. Here, we report the finding of dense beds of Mytilus growing in tidal lagoons and river mouths, where the availability of carbonate ions is remar...
Article
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A comparison was made of the hygroscopicity, cell wall chemical composition and crystallinity of recently peeled poplar (Populus spp.) wood and wood of the same species subjected to repeated cycles (20, 60 and 80) of vacuum/pressure (85 kPa/600 kPa) and soaking in an autoclave followed by oven drying. The 15 and 35 °C sorption isotherms were obtain...
Article
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Bone crystallite chemistry and structure change during bone maturation. However, these properties of bone can also be affected by limited uptake of the chemical constituents of the mineral by the animal. This makes probing the effect of bone-mineralization-related diseases a complicated task. Here it is shown that the combination of vibrational spe...
Article
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The morphology and ultrastructure of the shells of two balanid species have been examined, paying special attention to the three types of boundaries between plates: (i) radii-parietes, (ii) alae-sheaths, and (iii) parietes-basal plate. At the carinal surfaces of the radii and at the rostral surfaces of the alae, there are series of crenulations wit...
Article
The Guinea fowl eggshell is a bioceramic material with the remarkable mechanical property of being twice as strong as the chicken eggshell. Both eggshells are composed of 95% calcite and 3.5% organic matrix, which control its structural organization. Chicken eggshell is made of columnar calcite crystals arranged vertically. In the Guinea fowl, the...
Article
Obligate avian brood parasites lay stronger eggs than their hosts or non‐parasitic relatives because they are rounder and have a thicker eggshell. Additionally, some other characteristics of the brood parasitic eggshells related to their microstructure such as size and orientation of calcite crystal units could also contribute to generating even st...
Article
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Broiler chickens have an extreme physiology (rapid growth rates) that challenges the correct bone mineralization, being an interesting animal model for studying the development of bone pathologies. This work studies in detail how the mineralization, chemistry, and structural organization of tibiae bone in broiler chickens change with age during the...
Article
Unheated and heat-treated bovine bone material with differing heating times and heat-treated in increments from 100 °C to 1000 °C were analysed to study the crystallographic change of fresh biological bone mineral occuring during incineration. We combine several complementary analytical methods applied to the same unheated and heat treated material...
Article
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Avian (and formerly dinosaur) eggshells form a hard, protective biomineralized chamber for embryonic growth-an evolutionary strategy that has existed for hundreds of millions of years. We show in the calcitic chicken eggshell how the mineral and organic phases organize hierarchically across different length scales and how variation in nanostructure...
Article
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Food safety of table eggs is vital since many pathogens can contaminate the unfertilized egg, leading to increased risk of foodborne illness for consumers. The eggshell cuticle is the first line of defense to restrict the entry of egg-associated pathogens, such as Salmonella Enteritidis. The thickness and completeness of coverage of the cuticle lay...
Article
1. The cuticle is a very thin organic layer that coats the eggshell surface and plugs the eggshell pores preventing bacterial penetration. It also reduces egg shell permeability which is important to maintain egg internal quality. Thus, the eggshell cuticle quality is crucial to ensure the food safety and quality of eggs. 2. A new methodology to as...
Article
To achieve a better understanding of the formation and evolutionary history of molluscan shell microstructures, we analysed crystallographic textures of shell microstructures of selected species of Protobranchia, the most ancient group of Bivalvia. Our dataset covers four of the five protobranch superfamilies. Shell layers of five species of Nuculo...
Article
MamC from Magnetococcus marinus MC-1 has been shown to control the size of magnetite crystals in in vitro experiments, thereby demonstrating its potential as a candidate protein for the production of magnetite nanoparticles possibly useful in medical and other applications. However, the importance of the structure and aggregation state of the prote...
Article
Environmental gradients play an important role in shaping geographic variability in coastal marine populations. Thus, the ability of organisms to cope with these changes will depend on their potential to acclimatize, or adapt, to these new environmental conditions. In this study, we investigate the spatial variability in biological responses shown...