Alejandro Pérez-de-Luque

Alejandro Pérez-de-Luque
Instituto de Investigación y Formación Agraria y Pesquera | IFAPA · Departamento de Mejora Genética y Biotecnología

PhD
Plant resistance against pathogens and nanotechnology applied to crop protection and production.

About

95
Publications
44,302
Reads
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4,711
Citations
Citations since 2016
38 Research Items
2804 Citations
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20162017201820192020202120220100200300400500
Introduction
I finished agronomical engineering studies at the University of Córdoba (Spain) in 1998. My PhD was about parasitic plants, finished in 2002. My main researching topics include: 1) Resistance and defensive mechanisms of crops against parasitic plants and other pathogens; 2) Nanotechnology applied to plant research and agriculture; 3) Mycorrhizal fungi, rhizobacteria and induced resistance; 4) Influence of microbiological components of the soil in holm oak decline; 5) Plant histology and microscopy (histochemistry, confocal microscopy)
Additional affiliations
April 2013 - March 2015
The University of Sheffield
Position
  • Marie Curie Fellow
September 2009 - present
Instituto de Investigación y Formación Agraria y Pesquera
Position
  • Researcher
Description
  • Centro "Alameda del Obispo"
January 2008 - August 2009
Spanish National Research Council
Position
  • Contract Researcher "Ramón y Cajal" programme
Education
January 1999 - July 2002
University of Cordoba (Spain)
Field of study
  • Agronomical Engineering
September 1991 - April 1998
University of Cordoba (Spain)
Field of study
  • Agronomical Engineering

Publications

Publications (95)
Article
Full-text available
Yellow (stripe) rust, caused by the biotrophic fungus Puccinia striiformis f. sp. tritici (Pst), stands as the most serious wheat disease worldwide, affecting approximately 88% of world wheat production. Even though yellow rust generally develops in cool humid weather conditions, the expansion of new races adapted to warmer climates threatens zones...
Article
Full-text available
La seguridad alimentaria de los ciudadanos es una de las preocupaciones básicas de un país. En un contexto de población mundial en expansión y cambio climático global es un desafío para la sociedad mundial. ¿Qué papel pueden jugar los cereales en todo esto? ARTÍCULO DISPONIBLE ONLINE: https://theconversation.com/nuestra-seguridad-alimentaria-depe...
Article
Agriculture is responsible for supplying food, feed, fibres, and an increasing fraction of fuel and raw chemicals for industry. Fulfilling such demands sustainably is one of the major challenges of our time. In this metadata analysis, we offer a quantitative overview of how scientists have been addressing the effects of nanomaterials on plants betw...
Article
Full-text available
Wheat and rice are two main staple food crops that may suffer from yield losses due to drought episodes that are increasingly impacted by climate change, in addition to new epidemic outbreaks. Sustainable intensification of production will rely on several strategies, such as efficient use of water and variety improvement. This review updates the la...
Article
Full-text available
Aim of study: Septoria tritici blotch (STB), caused by the fungus Zymoseptoria tritici, is one of the most important wheat diseases worldwide, affecting both bread and durum wheat. The lack of knowledge about the interaction of durum wheat with Z. tritici, together with limited resources of resistant durum wheat material, have both led to a rising...
Preprint
Full-text available
Agriculture is responsible for supplying food, feed, fibres, and an increasing fraction of fuel and raw chemicals for industry. Fulfilling such demands sustainably is one of the major challenges of our time. In this metadata analysis of 10 years of published research, we offer a quantitative overview of how scientists have been addressing the effec...
Article
Drought is one of the most important constraints to crop productivity worldwide. Control of plant responses to drought is very complex. The mechanisms and their intensity may differ between species and/or genotypes ultimately conditioning tolerance or susceptibility. We explore here the strategy set up by two oat cultivars to cope with drought base...
Article
Full-text available
Agriculture must overcome several challenges in order to increase—or even maintain—production, while also reducing negative environmental impact. Nanotechnology, fundamentally through the development of smart delivery systems and nanocarriers, can contribute to engineering more efficient and less contaminant agrochemicals. This Collection presents...
Article
Full-text available
ISSN 1889-0776 Tierras de Castilla y León: Agricultura, 288:62-66
Article
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Nanotechnology is emerging as a very promising tool towards more efficient and sustainable practices in agriculture. In this work, we propose the use of non-toxic calcium phosphate nanoparticles doped with urea (U-ACP) for the fertilization of Triticum durum plants. U-ACP nanoparticles present very similar morphology, structure, and composition tha...
Presentation
Podcast del Programa de Radio "Investigadores por el Mundo" presentado y dirigido por Antonio G. Armas, número 78, que se emitió en directo el martes 12/05 en Libertad FM de 13 a 14 horas: https://t.co/NU9wN9Ze0R?amp=1
Article
Biomimetic calcium phosphate nanoparticles (CaP) have been actively used in biomedicine, due to their high biodegradability and biocompatibility. However, much less progress has been made regarding its application in precision agriculture, i.e., for the controlled delivery of active species to plants. Herein we report a straightforward and green sy...
Article
Full-text available
Purpose of Review Holm oak is a relevant species, both for its distribution and ecological importance. Among the risks looming over this species, oak decline—influenced by extreme climatic events, and alien-invasive species—is considered the main factor causing the loss of holm oak in Mediterranean open woodlands. The aim of this review is to ident...
Article
Full-text available
Las dehesas representan el ecosistema mediterráneo más extendido en la Península Ibérica, con la encina como especie protagonista. Actualmente, la sostenibilidad tanto de la especie como del ecosistema se encuentran en serio peligro debido al decaimiento de los encinares, producido por la podredumbre de raíz, causada por patógenos de suelo del géne...
Article
Full-text available
La encina (Quercus ilex subsp. ballota) es un árbol autóctono de la cuenca Mediterránea, considerada una especie clave por su importancia ecológica y socioeconómica. A nivel ecológico, destaca su capacidad para ocupar zonas que presentan condiciones extremas para otras especies arbóreas , considerándose una especie importante en la lucha contra la...
Article
Full-text available
El decaimiento de la encina es una enfermedad compleja, en la que intervienen numerosos factores como el cambio climático, o las modificaciones en el manejo de las explotaciones, aunque se considera que el factor más importante es la podredumbre de la raíz de la encina, asociada a algunos patógenos, principalmente a Phytophthora cinnamomi (conocida...
Article
Full-text available
Forest decline is nowadays a major challenge for ecosystem sustainability. Dehesas, which consists of savannah-like mediterranean ecosystems, are threatened by the holm oak decline in the south-west of Iberian Peninsula. Phytophthora cinnamomi is considered the main agent of holm oak root rot, but little is known about the relationship between dive...
Article
Natural compounds and living organisms still have a limited use in crop protection, and only a few of them have reached the market, despite their attractiveness and the efforts made in research. Very often those products have negative characteristics compared to the synthetic compounds, e.g. higher costs of production, lower effectiveness, lack of...
Article
Full-text available
El calabacín muestra resistencia a Meloidogyne incognita y susceptibilidad a M. javanica. Los procesos clave en el parasitismo que diferencian M. incognita de M. javanica son el paso del cuarto estado juvenil a hembra adulta y la menor fertilidad de las hembras grávidas de M. incognita . Los sitios de alimentación inducidos por M. incognita se degr...
Article
Full-text available
The sustainability of "dehesas" is threatened by the Holm oak decline. It is thought that the effects of root rot on plant physiology vary depending on external stress factors. Plant growth and biomass allocation are useful tools to characterize differences in the response to drought and infection. The study of physiological responses together with...
Article
Full-text available
Physical and chemical barriers in root tissues contribute to quantitative resistance to Fusarium oxysporum f. sp. pisi in pea Fusarium wilt caused by Fusarium oxysporum f. sp. pisi (Fop) is one of the most destructive diseases of pea worldwide. Control of this disease is difficult and it is mainly based on the use of resistant cultivars. While mon...
Article
The development of Meloidogyne incognita and M. javanica on zucchini 'Amalthee' was compared to characterise critical events in plant parasitism. Meloidogyne incognita was much less successful parasitising zucchini than M. javanica despite similarities in penetration rates and juvenile development. The increased frequency of undersized individuals,...
Article
Full-text available
Belowground interactions between plant roots, mycorrhizal fungi and plant growth-promoting rhizobacteria (PGPR) can improve plant health via enhanced nutrient acquisition and priming of the plant immune system. Two wheat cultivars differing in their ability to form mycorrhiza were (co)inoculated with the mycorrhizal fungus Rhizophagus irregularis a...
Article
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Copper-based chemicals are effectively used as antimicrobials in agriculture. However, with respect to its nanoparticulate form there has been limited number of studies. In this investigation, in vitro tests on effect of copper nanoparticles (CuNPs) against plant pathogenic fungi, oomycete, bacteria, beneficial microbes Trichoderma harzianum and Rh...
Chapter
Parasitic weeds could be considered as a separate world within the weed 'universe'. This is because of their unique systems that allow them to interact with the host plants in almost every phase of their life-cycle. This chapter considers parasitic weeds in Europe the nature of the problem, the unique features of their biology and implications for...
Article
Full-text available
The number of published researching works related with applications of nanomaterials in agriculture is increasing every year. Most of such works focus on the synthesis of nanodevices, their characteristics as nanocarriers for controlled release of active substances, and their interaction (either positive or negative) with plants or microorganisms u...
Research
Full-text available
http://masscience.com/2014/09/17/1949/ Este relato forma parte de una actividad de “cuentacuentos” realizada en Sheffield (Reino Unido) durante el Festival de la Mente (Festival of the Mind) en septiembre de 2014. El relato original estaba en inglés, pero lo he traducido para que los lectores de MasScience puedan también disfrutar de él. La idea er...
Research
Full-text available
http://masscience.com/2014/05/08/plantas-que-se-alimentan-de-otras-plantas/ El reino vegetal no suele estar entre los más utilizados cuando se trata de hacer divulgación científica. La verdad, es que una planta tiene poco que hacer cuando se trata de competir contra feroces dinosaurios o misteriosos agujeros negros para atraer la atención del públi...
Research
Full-text available
http://www.agenciasinc.es/Opinion/Cultivos-transgenicos-sufre-Europa-fatiga-de-decision En las dos últimas décadas, Europa ha estado inmersa en un debate sin fin acerca de si los cultivos genéticamente modificados debían ser incorporados al mercado, y por tanto, que entraran a formar parte de la cadena alimentaria. Seis investigadores de la Univer...
Article
Full-text available
Root parasitic weeds are a major limiting production factor in a number of crops, and control is difficult. Genetic resistance and chemical control lead the fight, but without unequivocal success. Models that help to describe and even predict the evolution of parasitism underground are a valuable tool for herbicide applications, and even could help...
Article
Full-text available
Wilt disease of lentil caused by Fusarium oxysporum f.sp. lentis (Fol) is one of the most important diseases affecting lentil worldwide. Differential response of six lentil accessions with reported differences in the level of resistance to Fol was studied micro and macroscopically. Penetration took place through root epidermal cells without formati...
Article
Full-text available
Aim A principal goal of grassland management is to minimize the use of artificial fertilizers by maximising the productivity of nitrogen-fixing leguminous plants such as clovers. The objective of this study was to investigate whether a plant-growth-promoting strain of the free-living rhizosphere fungus Trichoderma hamatum (GD12) could be used as a...
Article
Quercus ilex is one of the most susceptible European forest species to the oomycete Phytophthora cinnamomi, which causes root rot, contributing to holm oak decline, a particularly serious problem in the “dehesas” ecosystem of south western Iberian Peninsula. This work describes the pathogen-host interaction of Q. ilex and P. cinnamomi, using new in...
Article
Full-text available
For several years now, nanoscaled materials are being implemented in biotechnological applications related to animal (in particular human) cells and related pathologies. However, the use of nanomaterials in plant biology is far less widespread, although their application in this field could lead to the future development of plant biotechnology appl...
Chapter
This chapter focuses on applications of nanotechnology in the field of agriculture. The first part deals with nanoformulations of agrochemicals for applying pesticides and fertilizers to crops. Three types of nanomaterials are considered: organic polymers, inorganic compounds, and hybrid materials (nanocomposites). This is followed by a brief discu...
Chapter
Full-text available
The invasive phase of haustorium development starts when the young haustorium adheres to host roots by means of specialized attachment devices employing adhesive substances. A group of intrusive cells then enzymatically and mechanically penetrate host tissues. Enzymatic activity by the intrusive cells opens gaps between the host cells, while the el...
Article
Full-text available
Ascochyta blight caused by Didymella pino-des is a serious disease of pea (Pisum sativum ssp. sat-ivum) to which little resistance has been identified so far. Only incomplete resistance is available in pea germplasm although higher levels of resistance have been reported in related Pisum species. In this study we characterized histochemically the u...
Article
Full-text available
Phytophthora cinnamomi Rands. is an important root rot pathogen widely distributed in the north hemisphere, with a large host range. Among others diseases, it is known to be a principal factor in the decline of holm oak and cork oak, the most important tree species in the "dehesa" ecosystem of south-western Spain. Previously, the focus of studies o...
Data
Figure S1. Two series (A1-A5, B1-B5) of consecutive sections from vascular tissue of inoculated samples 14 dai. Observation of consecutive sections allows a three-dimensional reconstruction of the intracellular pathogen structures and confirms that the appearance of septate hyphae in a single section is an artefact. Bars present in figures A5 and B...
Data
Figure S2. Schematic representation for the sectioning of several neighboring hyphae. When observed in a slide, a single section could be confused with a septated hypha. For that reason, several consecutive sections must be checked in order to get a three dimensional representation of the tissues. H1, Hyphae 1; H2, Hyphae 2; H3, Hyphae 3; H4, Hypha...
Article
Full-text available
The behavior of nanoparticles inside plants is gaining importance for its implications in research about putative applications and toxicology. Magnetic carbon-coated nanoparticles can be easily traced through plant tissues using simple and affordable histological techniques. Here we present a methodology for the synthesis of such nanoparticles. We...
Article
The development of water-soluble nanodevices extends the potential use of compounds developed for other purposes (e.g. antifungal drugs or antibiotics) for applications in agriculture. For example, the broad-spectrum, water-insoluble, macrolide polyene antibiotic amphotericin B (AMB) could be used to inhibit phytopathogenic fungi. A new formulation...
Article
Full-text available
The development of nanodevices for agriculture and plant research will allow several new applications, ranging from treatments with agrochemicals to delivery of nucleic acids for genetic transformation. But a long way for research is still in front of us until such nanodevices could be widely used. Their behaviour inside the plants is not yet well...
Article
Broomrapes (Orobanche spp. and Phelipanche spp.) are weedy root parasites that represent a major constraint for faba bean (Vicia faba) cultivation in the Mediterranean area. Control methods are being developed that comprise techniques ranging from agronomical practices to precision farming, including chemical and biological control, genetic and ind...
Article
Full-text available
Orobanche and Phelipanche spp. (broomrapes) are parasitic plants that can be responsible for devastating losses in several important crops. The development of resistant cultivars is one of the key strategies in the fight against this pest. However, the nature of resistance is complex and the basis of the interaction between the host and the parasit...
Article
A comparative structure-activity relationship (SAR) study has been conducted with several guaianolide sesquiterpene lactones (SLs) as inducers of the germination of sunflower broomrape (Orobanche cumana) seeds. Compounds were selected and synthesized to study the influence of the lactone-enol-gamma-lactone moiety on the selectivity of SLs toward th...
Article
Background: Pea cultivation is strongly hampered in Mediterranean and Middle East farming systems by the occurrence of Orobanche crenata Forsk. Strategies of control have been developed, but only marginal successes have been achieved. Most control methods are either unfeasible, uneconomical, hard to achieve or result in incomplete protection. The...
Article
The field of nanotechnology opens up novel potential applications for agriculture. Nanotechnology applications are already being explored and used in medicine and pharmacology, but interest for use in crop protection is just starting. The development of nanodevices as smart delivery systems to target specific sites and nanocarriers for controlled c...
Article
Full-text available
In recent years, the application of nanotechnology in several fields of bioscience and biomedicine has been studied. The use of nanoparticles for the targeted delivery of substances has been given special attention and is of particular interest in the treatment of plant diseases. In this work both the penetration and the movement of iron-carbon nan...
Data
Correlative images 1. Bright and dark field visualization of the nanoparticles shown in Figure 2A: A) Bright field image, of the same field in (Fig. 2A). The nanoparticle aggregates appear as a dark material. B) Dark field image of the same field in (Fig. 2A). The nanoparticles appear as bright refringent. Bar = 40 μm
Data
Correlative images 2. Bright and dark field visualization, and electron microscopy of the nanoparticles shown in Figure 3B. A) Dark field image, B) Bright field image, C) Electron micrograph of the nanoparticle aggregates of (A) and (B). Bar in A and B = 50 μm; C = 2 μm.
Data
Correlative images 4. Bright and dark field visualization of the nanoparticles shown in Figure 7A. A) bright field image. B) dark field image. Bar = 30 μm.
Data
Correlative images 3. Detail of the cell squared in fig 4A. A) Phase contrast image, showing nanoparticle aggregates that are visible as an intense dark material. The cytoplasm appears dense and displaying numerous structures and organelles in comparison with the surrounding cells. B) Same field that in (A), bright field image. Bar = 20 μm.
Article
A number of broomrapes (Orobanche spp.) are major threats for grain legumes in the Mediterranean area. Previous studies have shown very high levels of resistance to Orobanche crenata in Medicago truncatula with little variation among accessions hampering, therefore, its use for genetic analysis. In order to identify alternative systems for ulterior...
Article
Over 4000 species of angiosperms are able to directly invade and parasitise other plants, but only very few are weedy and parasitise cultivated plants. Together with the witchweeds (Striga spp.) and dodders (Cuscuta spp.), the broomrapes (Orobanche spp.) affect important crops causing complete yield loses with severe infestations. Genetic resistanc...
Article
Full-text available
Various Orobanche species are weedy and cause severe reduction in the yields of many important crops. The seeds of these parasitic weeds may remain dormant in the soil for many years until germination is stimulated by the release of a chemical signal from a host plant. In order to determine the effects of fenugreek root exudate on the induction of...
Article
The weedy root parasite Orobanche crenata constitutes a serious threat to lentils and other legumes grown in the Mediterranean and western Asia. Control strategies have centred on agronomic practices and the use of herbicides. Resistance breeding is hampered by scarcity of proper sources of resistance and of a reliable and practical screening proce...
Article
Full-text available
The weedy root parasite Orobanche crenata constitutes a serious threat to lentils and other legumes grown in the Mediterranean and western Asia. Control strategies have centred on agronomic practices and the use of herbicides. Resistance breeding is hampered by scarcity of proper sources of resistance and of a reliable and practical screening proce...
Article
Full-text available
The great potential of using nanodevices as delivery systems to specific targets in living organisms was first explored for medical uses. In plants, the same principles can be applied for a broad range of uses, in particular to tackle infections. Nanoparticles tagged to agrochemicals or other substances could reduce the damage to other plant tissue...
Article
Full-text available
A number of broomrapes (Orobanche spp.) are major threats for grain legumes in the Mediterranean area. Previous studies have shown very high levels of resistance to Orobanche crenata in Medicago truncatula with little variation among accessions hampering, therefore, its use for genetic analysis. In order to identify alternative systems for ulterior...
Article
Crenate broomrape (Orobanche crenata) is a root parasitic weed that represents a major constraint for grain legume production in Mediterranean and West Asian countries. Medicago truncatula has emerged as an important model plant species for structural and functional genomics. The close phylogenic relationship of M. truncatula with crop legumes incr...
Article
10 pages, 4 figures. Broomrapes (Orobanche spp.) are parasitic angiosperms, which attach to the roots of the hosts to take water and nutrients from them. No complete control measures are available to date, but breeding for resistance remains as one of the most feasible and environmentally friendly methods. However, the mechanisms governing the inte...
Article
Orobanche foetida Poir. is a parasitic plant widely distributed in the Western Mediterranean area. It typically parasitizes wild plants but has recently been described as an agricultural problem in legume crops in Tunisia. The pattern of genetic variation within and among O. foetida populations growing on chickpea and faba bean was analyzed by RAPD...