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Publications (55)
Riassunto. Vengono descritte le xerofite ita-liane di nuova introduzione nell'area espositi-va denominata il "Deserto" dell'Orto Botanico di Napoli. Queste specie vengono discusse per loro aspetti tassonomici, per il significato didattico e divulgativo che rivestono e per la loro importanza per la conservazione ex situ di specie vegetali di specifi...
A method based on the use of electromagnetic radiation (EMR) in the radiofrequency band was experimented to eliminate heterotrophic organisms from biofilms encrusting linen canvasses used for paintings. Artificial biofilms were used for exposure to EMR. Biofilms were grown on fragments of canvasses sampled from the backside of a painting. The fragm...
A novel technique, effective in eliminating biodeteriogens from biofilms encrusting terracotta artifacts, is presented here. This method is based on the use of high-strength electromagnetic radiation (EMR) in the radiofrequency band. Shards of terracotta from historical pots at the Botanical Garden of Naples, Italy, were used. The shards, after ste...
Heterotrophic components of biofilms on wood artefacts were studied at the Conservation Laboratory for Wood Artefacts of the University Suor Orsola Benincasa of Naples, Italy. The aim of the study was to add new information on the micro-habitats represented by biofilms formed by wood-dwelling organisms. Light and electron microscopy of histological...
We investigated microorganisms dwelling on rocks, walls and paintings in two votive chapels built in grottoes in the Region of Campania, Italy. One grotto was near the coast in an area with a Mediterranean climate, and the other grotto was inland on a mountain in an area with a cold continental climate. Color and distribution of biofilms in various...
Methodologies used by the authors in the study of biofilms encrusting artifacts, monuments and archaeological sites are illustrated. Techniques adopted in each step of the study, i.e., sampling, identification of biofilm microbial components, analysis of inorganic and organic components of biofilms and removal of biodeteriogens by using radio frequ...
Abstract. Ceratozamia delucana Vázquez-Torres, Moretti & Carvajal-Hernández (Zamiaceae) is described from a mountain cloud forest in Veracruz, Mexico. The new species differs from others in the genus by larger sizes of leaves, petioles and leaflets. This taxon is apparently related to C. mexicana, C. latifolia and C. morettii. Like in most Ceratoza...
We investigated phototrophic microorganisms dwelling on stone walls made of Piperno, a volcanic rock frequently used as construction material in historical buildings in Naples, Italy. Biofilms from three historical buildings in the center of the city and from a natural Piperno quarry located in a suburban area were examined. Light and electron micr...
A methodology aiming at identifying and removing biofilms from cultural heritage was applied to stones from tuff walls in historical sites. Identification of phototrophic encrusting microorganisms was carried out by optical and electron microscopy, as well as by molecular techniques (DNA analyses and denaturing gradient gel electrophoresis (DGGE))....
A scanning electron microscopy (SEM) study of Cycas cuticle characteristics was undertaken in order to expand our knowledge of microscopic characters not observable under light microscopy and to clarify unresolved affinitites among some species within the genus. Whole leaf and isolated cuticle specimens from the middle region of leaflets of greenho...
Phylogenetic relationships among 17 taxa of Dipsacaceae were inferred from nucleotide sequence variation in both the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA and the chloroplast trnL(UAA) intron sequences. The combined phylogenetic analysis, carried out by using two taxa from Valerianaceae as an outgroup yielded a single m...
This book presents the current state of our knowledge of the systematics of the approximately 300 species of cycads. It has been developed from 15 papers presented at the conference in Miami. Guidelines are presented for the designation of species, species boundaries and species groupings, thus clarifying what has been a confused area of research....
Cyanidium caldarium, Cyanidioschyzon merolae and Galdieria sulphuraria are three unicellular algae characteristic, of acid thermal environments. Recently, on the basis of morphological characters, three new species of Galdieria (G. partita, G. daedala, G. maxima ) isolated from acid-thermal springs in Russia have been instituted. A selected region...
Phylogenetic relationships among members of genus Orchis and allied genera Aceras, Anacamptis, Barlia, Dactylorhiza, Gymnadenia, Himantoglossum, Neotinea, Ophrys, Platanthera, and Serapias were inferred from nucleotide sequence variation in the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA. Sequences were subjected to various a...
Light and scanning electron microscopy were utilised to study stoma and cuticle morphology whereas transmission electron microscopy was used to observe plastid ultrastructure in Ceratozamia kuesteriana Regel (Zamiaceae). Results show that in G kuesteriana a diperigenous‐type stoma (or a derivation of a diperigenous type) occurs and that protein cry...
A new species of Zamia (Zamiaceae) from Belize and the Yucatan Peninsula of Mexico
Chromosome numbers and karyotypes of four species of Zamia L. (Zamiaceae) are described. Plants of Z. manicata from Colombia are 2n = 18 with eight metacentric (M), four submetacentric (S), two acrocentric (A), and four telocentric (T) chromosomes. Plants of Z. ipetiensis from Panama are 2n = 23 with 3M + 4S + 2A + 14T. Plants of Z. cunaria from Pa...
Chromosome numbers and karyotypes of four species of Zamia L. (Zamiaceae) are described. Plants of Z. manicata from Colombia are 2n = 18 with eight metacentric (M), four submetacentric (S), two acrocentric (A), and four telocentric (T) chromosomes. Plants of Z. ipetiensis from Panama are 2n = 23 with 3M + 4S + 2A + 14T. Plants of Z. cunaria from Pa...
A phylogenetic analysis of all the intrageneric taxa of the genus Dioon Lindley (Zamiaceae) was undertaken by using chloroplast DNA restriction fragment length polymorphism analysis. Wagner parsimony analysis on a 187 character matrix yielded two equally parsimonious trees, differing only for the position of D. caputoi. The consensus tree has two w...
A phylogenetic analysis of all the intrageneric taxa of the genus Dioon Lindley (Zamiaceae) was undertaken by using chloroplast DNA restriction fragment length polymorphism analysis. Wagner parsimony analysis on a 187 character matrix yielded two equally parsimonious trees, differing only for the position of D. caputoi. The consensus tree has two w...
The effect of various oxygen tensions on Acetylene Reduction Activity (ARA) by symbiotic and asymbiotic diazotrophs was examined in the range 0–59% O2. For each diazotroph, ARA always appeared bimodal. An ARA maximum occurred at different oxygen concentrations, namely at 11% O2in Alnus cordata. 9% in Alnus glutinosa. 15% in Colletia paradoxa. 17% i...
Intraspecific chromosome variation in three species of Zamia (Zamiaceae, Cycadales) from Mexico and Belize is reported. Zamia fischeri has 2n = 16 and 18, Z. picta has 2n = 21 and 22, and Zamia sp. nov. has 2n = 22 and 26. All cytotypes have two submetacentric and two acrocentric chromosomes but differ in number of metacentric and telocentric chrom...
Chromosome numbers and karyotypes of species from four American Zamiaceae (Cycadales) are reported. Zamia shows interspecific and intraspecific chromosome variation, whereas Microcycas, Ceratozamia, and Dioon have constant karyotypes within each genus. In Zamia, all karyotypes have the same number of submetacentric and acrocentric chromosomes, but...
The chromosome numbers of several species ofZamia from Mexico are reported.Z. paucijuga, distributed from central Oaxaca to Nayarit, has been found to have 2n = 23, 25, 26, 27 and 28. 2n = 28 is the highest chromosome number yet found in the cycads. Karyotypes of this species differ principally in the number of telocentric and metacentric chromosom...
Seventeen strains comprising six species of Azolla were studied. A. filiculoides was the most suitable for green manure in temperate climates. The endophytes of all species appeared to be identical in morphology although heterocyst frequency varied.
Cycasin and macrozamin, the main methylazoxymethanol glycosides, found in cycads, have been analysed in seeds of representative species of all 10 genera of this group. Macrozamin concentration is taxonomically significant at the generic level. The taxonomic and evolutionary significance of the results is discussed.
Cyanidium caldarium Geitler, Cyanidioschyzon merolae De Luca, Taddei & Varano and Galdieria sulphuraria (Galdieri) Merola are the three thermoacidophilic algae characterized by a chloroplast which is bounded by a single membrane. The presence of this atypical chloroplast made the inclusion of these algae in the Rhodophyta difficult. The discovery i...
The patterns of monosaccharide distribution of the mucilages of Cycadales are characteristic at the generic level. Arabinose, fucose, galactose, mannose, rhamnose and methylrhamnose were identified in the hydrolysed mucilage of Bowenia, Ceratozamia, Cycas, Dioon, Encephalartos, Lepidozamia, Macrozamia, Microcycas and Zamia. Stangeria contains no rh...
Ceratozamia kuesteriana Regel, which was described from material coming from an unspecified Mexican locality and which, after its description, was
never recorded in the field, has been found in southern Tamaulipas.Ceratozamia kuesteriana is very closely allied toC. zaragozae Medellin but differs by not having twisted leaves.
The taxonomic attribution of Porphyridium Näg. to Rhodophyta is a question from a long time debated since Porphyridium lacks both the morphological and the reproductive features of the red algae.In the present paper we report the presence of floridosides and γ-linolenic acid in P. aerugineum and P. cruentum. Both these compounds are believed to be...
Acetylene reduction by detached nodules of Alnus cordata, A. glutinosa and Colletia paradoxa was tested with respect to the effect of temperature on nitrogenase activity. Nodules were incubated at temperatures from 5°C to 45°C, exposed to C2H2 generally for 1 hr, and sampled for C2H4. The maximum of activity occurred in all three species tested at...
Arabinose, fucose, galactose, mannose, methylrhamnose and rhamnose were identified in the hydrolysed mucilages of 14 Encephalartos species. The monosaccharides occur in quantities varying between 44% (w/w) in E. hildebrandtii and 89% (w/w) in E. tegulaneus. Galactose, arabinose and rhamnose are the most abundant monosaccharides. The monosaccharide...
A new species ofDioon has been found in Oaxaca. It is characterized by leaflets which are entire, lanceolate but unequally tapering toward the
apex and over 10 mm wide at the middle portion of the frond.
Megagametophytes of Ceratozamia mexicana, Cycas revoluta and Encephalartos umbeluziensis were induced to proliferate in vitro.Callusing was observed in the three species; regeneration occurred only in Ceratozamia mexicana and Cycas revoluta.Megagametophytes of Ceratozamia mexicana produced adventive embryos on White's basal medium. They became mono...
