Alberto Piloni

• Doctor of Philosophy
• Research Scientist at Polynovo

Successful preclinical drug testing relies in part on data generated using in vitro cell culture models that recapitulate the structure and function of tumours and other tissues in vivo. The growing evidence that 3D cell models can more accurately predict the efficacy of drug responses compared to traditionally utilised 2D cell culture systems has...

Three-dimensional (3D) bioprinting has revolutionised the field of biofabrication by delivering precise, cost-effective and a relatively simple way of engineering in vitro living systems in high volume for use in tissue regeneration, biological modelling, drug testing and cell-based diagnostics. The complexity of modern bioprinted systems requires...

Three-dimensional (3D) bioprinting has revolutionised the field of biofabrication by delivering precise, cost-effective and a relatively simple way of engineering in vitro living systems in high volume for use in tissue regeneration, biological modelling, drug testing and cell-based diagnostics. The complexity of modern bioprinted systems requires...

We apply Brillouin microscopy to characterize micromechanical properties of 3D bioprinted hydrogel constructs. Non-contact and label-free mapping of constructs’ mechanical properties in situ provides invaluable insight into spatial distribution and swelling behavior of hydrogels.

Recently the role of protein absorption in nanoparticle drug delivery has gathered significant attention as the protein corona can significantly decide on the fate of nanoparticles in the body. Although it is known that the surface chemistry will significantly influence the amount and type of bound protein, there is little known about the effect of...

Poly(vinyl pyridine) has widely been used as a pH‐responsive polymer to trigger changes in self‐assembly of block copolymer micelles. However, the polymer is known to display toxic features, which limits its ultimate applicability for biological applications. Here, poly(4‐vinyl imidazole) (P4VIm), a much more biocompatible polymer, is used as a pH‐...

Fluorine‐19 (¹⁹F)‐based contrast agents are increasingly used for magnetic resonance imaging. Conjugated to polymers, they provide an excellent quantitative imaging tool to detect the movement of the polymeric nanoparticles in vivo as there is no background signal in tissue. One of the challenges is the decline in signal intensity when the conjugat...

Amphiphilic block copolymers can elegantly assemble in water to form well defined nano-object and through intellligent design or the polymers it is possible to efficiently prepare functional materials for biomedical applications such as drug carriers. Linear triblock terpolymers add an additional level of complexity to the process. The assembly can...

It has been hypothesized that maximum binding of carbohydrates can be achieved when the distance between two carbohydrate units are equivalent to two binding sites of a lectin. Therefore, a library of glyco starpolymers was prepared using RAFT polymerization to study the interaction with Concanavalin A (Con A). The starpolymers had a block structur...