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Introduction
Current institution
Publications
Publications (94)
Study question
Does chemotherapy (CT) treatment for testicular cancer induce sperm methylome changes?
Summary answer
The analysis of 27 pre- and post-CT samples from testicular cancer survivors identified 65 differentially methylated regions.
What is known already
Research has shown that cancer treatment can cause DNA damage and aneuploidy in spe...
Study question
Are fragmentation levels (single-double stranded DNA breaks) or aneuploidy frequencies in sperm increased in patients with testicular cancer who recovered spermatogenesis after chemotherapy (CT)?
Summary answer
Similar levels of sperm DNA fragmentation and sperm aneuploidy frequency are found in men with testicular cancer pre- and p...
Information regarding the effects of the novel coronavirus on human reproduction is currently limited. The objective of our work was to assess whether, in women who have passed the disease, there was a variation in the ovarian reserve through the determination of AMH levels. During May-June 2020, women performing an Assisted Reproductive treatment...
Research question:
There is some controversy regarding the impact of ovarian stimulation on immune cells in women undergoing IVF. The study's aim was to determine whether ovarian stimulation affected immune uterine cells in healthy women undergoing IVF.
Design:
This prospective cohort study included 28 patients undergoing IVF and 47 healthy oocy...
Research question:
Is anti-Müllerian hormone (AMH) serum concentration a useful tool to predict the outcome of assisted reproductive treatment?
Design:
Retrospective cohort study involving 2971 patients who underwent 5570 IVF cycles. Patients were classified into six groups according to their AMH levels and analysed for associations with reprodu...
The objective of our study was to assess if sperm DNA fragmentation could affect embryo quality and the morphokinetic parameters of embryo development
Recently, growing interest in vitamin D has emerged from findings that demonstrate a low vitamin D status in populations. Similarly, much interest has been shown in the role that anti-Müllerian hormone (AMH) plays in reproductive physiology. Considerable confusion as to whether vitamin D status is related to ovarian function can be found in the lit...
Purpose of review:
Biomarkers are useful tool to help clinicians to estimate the chances of pregnancy of an infertile couple prior to assisted reproductive treatment. One proposed candidate is anti-Müllerian hormone (AMH), but its role as a predictor of reproductive success, especially in patients with low or very low levels, is under discussion....
Objective:
To determine whether the type of gonadotropin affects the secretion of oocyte-specific factors, the endocrine pattern in follicular fluid, and the apoptosis rate in cumulus cells.
Study design:
Prospective and observational study into an university-affiliated private in vitro fertilization setting. Ninety women included in our oocyte...
To determine the relationship between serum P levels on the day of hCG administration and ongoing pregnancy rates.
Retrospective study.
University-affiliated private IVF setting belonging to IVI group in Spain.
A total of 2,458 couples undergoing IUI.
Ovarian stimulation with human recombinant FSH.
Ongoing pregnancy and miscarriage rates.
Progester...
Objective
To investigate the correlation between total and bioavailable serum 25-OH vitamin D and the pregnancy rate in recipients of donated oocytes.
Design
Retrospective study.
Setting
University-affiliated private IVF center.
Patient(s)
A total of 267 patients who were referred to our clinic for oocyte donation from June 2013 to December 2013...
The role of Na(+) fluxes through voltage-gated sodium channels in the regulation of sperm cell function remains poorly understood. Previously, we reported that several genes encoding voltage-gated Na(+) channels were expressed in human testis and mature spermatozoa. In this study, we analyzed the presence and function of the TTX-resistant VGSC α su...
Study question
The aim of this prospective study was to analyze the utility of MACS (magnetic activated cell sorting) technique to improve pregnancy rates in ICSI cycles using sperm samples from patients with high level of sperm DNA fragmentation.
Summary answer
Sperm magnetic separation is a very useful technique to improve the pregnancy rate in...
Objective:
To investigate the effect of testosterone and hCG on FSH receptor (FSHR) protein and mRNA expression in human granulosa cells (GC) in vitro.
Study design:
Experimental in vitro cell culture obtained from healthy women undergoing IVF/ICSI due to male factor infertility. Human follicular fluid samples were obtained and after cumulus-ooc...
To study the levels of four markers of oxidative stress in follicular fluid (FF) and plasma of patients with infertility related to endometriosis and controls.
Experimental study.
University-affiliated hospital and infertility center.
Ninety-one infertile women were included in the study (23 infertile women with endometriosis and 68 controls includ...
The aneuploidy rate is higher in poor-quality sperm samples, which also have higher DNA fragmentation index values. The aim of this study was to assess the relationship between sperm DNA fragmentation in samples from infertile men belonging to couples with recurrent miscarriage or implantation failure and the aneuploidy rate in spermatozoa as well...
Introduction: In order to optimize IVF/ICSI treatments it is important that embryos are scored uniformly by different people working in
the same laboratory. Lab to lab standardisation for embryo morphological assessment is important for the comparison of results
and for doing research. To minimize the inter observer variability, there is a need of...
A prospective study was performed to assess the impact of sperm DNA fragmentation on the outcome of IVF with own or donated oocytes. The study population included 178 couples (62 cycles of IVF, 116 of intracytoplasmic sperm injection (ICSI)) with own (n=77) and donor (n=101) oocytes. DNA fragmentation was evaluated by TdT (terminal deoxynucleotidyl...
For abstracts see the DOI resource.
https://doi.org/10.1093/humrep/26.s1.75
This study evaluated the differentially modulated expression of vascular mediators in oocyte donors after hCG vs. GnRHa triggering, trying to understand ovarian hyperstimulation syndrome pathophysiology. Donors who received GnRH agonist triggering showed a statistically significant decrease in vascular endothelial growth factor in follicular fluid...
Introduction
In France, stringent laws mandate that oocyte donors are unpaid and fertile ( ≥ 1 child). As these rigid rules impede with the recruitment of donors, we accepted that women who wore progestin-delivering IUDs (Mirena®) and implants (Implanon®) donated their oocytes, starting in 1/2008. This was based on a preliminary report in 8 women i...
It remains unclear how GnRH agonist (GnRHa) triggering affects the luteal phase, so we investigated the luteal phase after GnRHa triggering, supported with conventional E(2)/P with or without low-dose hCG. E(2)/P support, compared with low-dose hCG, induced a shorter luteal phase (11.2 ± 1.1 vs. 15.0 ± 1.6 days) and fewer subjective complaints (0 v...
In assisted reproduction treatments it is no longer enough to obtain spermatozoa, we must find the best spermatozoa. Among the different parameters studied until recently, such as concentration, motility and morphology, varying success rates were observed even with similar classical sperm quality markers. Thus, research has been developed in order...
In assisted reproduction treatments it is no longer enough to obtain spermatozoa, we must find the best spermatozoa. Among the different parameters studied until recently, such as concentration, motility and morphology, varying success rates were observed even with similar classical sperm quality markers. Thus, research has been developed in order...
Introduction:
Ovarian Hyperstimulation Syndrome (OHSS) is a severe health complication observed in some patients undergoing controlled ovarian
stimulation (COS) during IVF.
However prediction of OHSS prior to controlled ovarian stimulation in an individual IVF treatment cycle using only age and
body mass index(BMI) remains a difficult task Monito...
Introduction:
Kartagener's syndrome (KS) is characterized by the classic triad of chronic sinusitis, bronchiectasis and situs inversus.
This is caused by dynein arm defects in axonemal microtubules in ciliary and flagellar structures. Male patients are invariably
infertile because of the immotility of spermatozoa. We report a healthy birth that wa...
Introduction
Recent publications suggest that elevated serum progesterone levels at the start of controlled ovarian stimulation (COS) were associated with decreased pregnancy rates following in vitro fertilization (IVF) using gonadotropin-releasing hormone (GnRH). The reference arm of a recent, randomized, double-blind, multicenter, international c...
To assess if the luteinizing hormone/human chorionic gonadotropin present in some gonadotropin formulations may be of benefit in protocols with GnRH antagonists.
Open, quasi-experimental, multicenter, prospective, parallel-controlled study compared 136 women undergoing in vitro fertilization--intracytoplasmic sperm injection after stimulation with...
This study evaluated the efficacy of a gonadotrophin-releasing hormone antagonist (GnRHa) to prevent premature luteinization (PL) and examined its impact on intrauterine insemination (IUI) cycle outcome. A total of 662 patients who were undergoing IUI were evaluated. Ovarian stimulation was started on day 3 with recombinant (r)FSH, followed by the...
La fragmentació del DNA és la conseqüència final de l'apoptosi. En el testicle és ben sabut
que el camí utilitzat per controlar el nombre adequat de cèl·lules germinals mitjançant
cèl·lules de Sertoli depèn de la via Fas. Tot i això, hi ha espermatozoides que han estat assenyalats
per prendre la via de l'apoptosi, que s'escapen d'aquest procés. Alt...
Introduction
Anti-müllerian hormone (AMH) has been recently introduced as a new marker of ovarian reserve with a high sensitivity and with the added value of being independent of cycle day. Some authors have claimed that it may also provide information regarding oocyte quality. As endometriosis is a disease that mainly affects the ovary and thus, e...
In a prospective cohort study, we compared the effect of hCG and GnRH agonist triggering of final oocyte maturation on vascular endothelial growth factor production. Vascular endothelial growth factor follicular fluid concentration was significantly lower in response to GnRH agonist versus hCG, which may partially explain the absence of OHSS in the...
Continuation of GnRH analogue after ovum retrieval produces a faster decline in vascular endothelial growth factor levels and ascitis in women at risk of ovarian hyperstimulation syndrome.
Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic complication of ovarian stimulation, and the pathophysiological mechanisms that trigger the syndrome remain unknown. HCG increases serum vascular endothelial growth factor (VEGF) concentrations, and VEGF modulates transendothelial permeability via endothelial adherens junctions, a downstream...
To compare the hormonal profile when using triptorelin vs. human chorionic gonadotropin (hCG) to trigger ovulation in intrauterine insemination (IUI) cycles.
Twenty-five patients who underwent 48 cycles were enrolled in a prospective, randomized, crossover study. After ovulation induction with recombinant follicle-stimulating hormone (FSH), couples...
To evaluate whether methotrexate (MTX) compromises ovarian reserve and future reproductive outcome in women undergoing assisted reproductive technology (ART), when it is used as first-line treatment for ectopic pregnancy (EP).
Prospective, observational study.
University-affiliated private IVF unit.
Twenty-five women undergoing IVF-ICSI who were tr...
Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic complication of treatment with fertility drugs. It is characterized by increased vascular permeability and simultaneous overexpression of vascular endothelial growth factor (VEGF) in ovarian cells.
We tested the hypothesis that the endothelium and endothelial cell-to-cell junctions are downs...
To evaluate the impact of aromatase inhibitors as adjuvant treatment in IVF cycles on intraovarian androgens and cycle outcome.
Observational, pilot study.
University-affiliated IVF unit.
One hundred forty-seven low responder patients with a previous canceled IVF cycle; 71 patients were treated with letrozole 2.5 mg plus a high-dose FSH/hMG-antagon...
Coasting, or withholding gonadotrophin administration while maintaining gonadotrophin-releasing hormone analogue until oestradiol drops to a safe concentration, is an alternative approach to prevent ovarian hyperstimulation syndrome (OHSS) in high responder patients. However, the length of this procedure has not been precisely studied. This paper i...
This study was conducted to investigate the mechanisms by which coasting may be effective in decreasing the incidence of ovarian hyperstimulation syndrome (OHSS).
A total of 160 women (patients and oocyte donors) undergoing coasting and 116 controls were included in the study. Serum, follicular fluid and granulosa cells were collected on the day of...
The CD3 subunits of the T cell receptor-CD3 complex (TCR-CD3) help to regulate surface TCR-CD3 expression, and participate in signal transduction leading to intrathymic selection and peripheral antigen recognition by T lymphocytes. Humans who lack individual CD3 chains show impairments in the expression and activation-induced downregulation of TCR-...
The contribution of CD3gamma to the surface expression, internalization, and intracellular trafficking of the TCR/CD3 complex (TCR) has not been completely defined. However, CD3gamma is believed to be crucial for constitutive as well as for phorbol ester-induced internalization. We have explored TCR dynamics in resting and stimulated mature T lymph...
The use of GnRH agonists (GnRHa) to induce final oocyte maturation and ovulation in intrauterine insemination (IUI) has been less extended than hCG. The more physiologic approach inducing an endogenous LH surge and their shorter half life makes them an appealing alternative while diminishing the incidence of OHSS. The objective of this study was to...
CD3 proteins may have redundant as well as specific contributions to the intracellular propagation and final effector responses of TCR-mediated signals at different checkpoints during T cell differentiation. We report here on the participation of CD3 gamma in the activation and effector function of human mature T lymphocytes at the antigen recognit...
Common variable immunodeficiency (CVID) is a very frequent but heterogeneous syndrome of antibody formation. The primary defect remains unknown, but many reports describe peripheral blood T lymphocyte dysfunctions in a substantial proportion of CVID patients, which may impair T--B cell collaboration. In order to investigate whether such putative de...
Caco-2 is a colonic tumour cell line which, when cultured, spontaneously exhibits enterocyte-like characteristics. Given the difficulties in maintaining long-lasting cultures of enterocytes, this cell line may be a suitable in vitro model to carry out experiments trying to delineate the involvement of enterocytes in local immune responses, and thei...
In the genetic disorder ataxia telangiectasia (AT), humoral (B) and cellular (T) immunological abnormalities are frequently observed. As a consequence, AT patients are predisposed to life-threatening sinopulmonary infections. The pathogenic mechanisms remain unknown, but a role for ATM in signal transduction from membrane receptors has been propose...
Human and murine CD3 deficiencies provide insight into the essential and redundant roles of CD3 proteins in T-cell development and immature T-cell function. Mature T-cell lines derived from a patient with CD3 gamma deficiency suggest that CD3 gamma participates in the induction of interleukin-2 (IL-2) and that the T-cell receptor (TCR)-CD3 complex...
CD3 chains associated with calnexin or with TCR molecules are first expressed and used by T cells early during their intrathymic development. Accordingly, CD3 deficiencies strongly influence early T-cell differentiation events, including the up-regulation of the TCR/ CD3 complex itself. Available data from human and murine CD3 deficiencies indicate...
Mature CD4(+) and CD8(+) T lymphocytes are believed to build and express essentially identical surface alphabeta T-cell receptor-CD3 (TCR.CD3) complexes. However, TCR.CD3 expression has been shown to be more impaired in CD8(+) cells than in CD4(+) cells when CD3gamma is absent in humans or mice. We have addressed this paradox by performing a detail...
Mature CD4+ and CD8+ T lymphocytes are believed to build and express essentially identical surface αβ T-cell receptor-CD3 (TCR·CD3) complexes.
However, TCR·CD3 expression has been shown to be more impaired in CD8+ cells than in CD4+ cells when CD3γ is absent in humans or mice. We have addressed this paradox by performing a detailed phenotypical and...
The biologic role of each CD3 chain and their relative contribution to the signals transduced through the TCR/CD3 complex and to downstream activation events are still controversial: they may be specialized or redundant. We have immortalized peripheral blood CD4+ and CD8+ T lymphocytes from a human selective CD3 gamma deficiency using Herpesvirus s...
CD3 gamma, a subunit of the T cell receptor-CD3 (TCR/CD3) complex, helps to support surface TCR/CD3 expression and participates in signal transduction for gene induction after antigen recognition by T lymphocytes, and in TCR/CD3 down-modulation. Humans with primary immunodeficiencies caused by inherited mutations in the CD3 gamma gene or in the gen...
The characterization of T cell immunodeficiencies could in part be supported by using stable cell lines in which biochemical and molecular studies of the defect could be carried out thereby omitting frequent bleeding of patients. First attempts to obtain such cell lines included HTLV-I transformation and exogenous IL-2 administration, but both mode...
Herpesvirus saimiri (HVS), an agent that can infect many human cell types, has been shown to immortalize selectively TCR alpha beta + CD3+ T lymphocytes. Human T cell precursors defined as CD34+CD3-CD4-CD8- were isolated from thymic samples and exposed to HVS in the presence of either IL-2 or IL-7. Cultures lacking the virus were non-viable by day...
Herpesvirus saimiri (HVS), an agent that can infect many human cell types, has been shown to immortalize selectively TCR αβ+CD3+ T lymphocytes. Human T cell precursors defined as CD34+CD3−CD4−CD8− were isolated from thymic samples and exposed to HVS in the presence of either IL-2 or IL-7. Cultures lacking the virus were
non-viable by day 15. Test c...
A 2-year-old female with important signs of immune response failure against virus, bacteria, fungi and protozoa and no obvious humoral or lymphocyte phenotypical defect was studied. Both peripheral blood mononuclear cells and IL-2-dependent T cell lines derived from the patient showed a severe selective T cell activation impairment via CD2, CD3 and...
Cytomegalovirus (CMV) infection is reported to cause transient immunosuppression in man. In this study we have analysed the effect of CMV on T lymphocyte function in 29 children diagnosed for acute CMV infection. Peripheral blood mononuclear cells (PBMC) obtained from the patients showed a significant specific impairment in their proliferative resp...
Extended HLA haplotypes frequencies were estimated from the HLA, C2, Bf and C4 phenotypes of 74 patients with non-insulin-dependent diabetes (NIDD), 92 with juvenile rheumatoid arthritis (JRA), 44 with Berger's disease (BD), 83 with insulin-dependent diabetes (IDD), and 140 healthy controls. The extended HLA haplotype B18 CF130 DR3 DQw2, which is c...
The selection of T lymphocytes in the thymus and their activation upon the encounter with foreign antigens in the periphery require the aggregation and signals of the T cell receptor (TcR)/CD3 complex and several surface molecules termed coreceptors (notably CD4 or CD8 and CD45). The spatial arrangement and interactions of the different molecules i...
A selective CD3 gamma defect, involving point mutations in both the paternal and the maternal genes, has been analyzed. The CD3 gamma defect affected two brothers of a four sibs family; one of them died at the age of 3 of a viral pneumonia with concomitant autoimmune features (Haemolytic anaemia and gut epithelial cell autoantibodies), whereas the...
