Alaattin SEN

Alaattin SEN
Abdullah Gul University · Department of Molecular Biology and Genetics

Ph.D.

About

107
Publications
10,201
Reads
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971
Citations
Introduction
Current work is primarily in three areas: 1) multi-targeting neuroinflammation and cognitive functions in multiple sclerosis applying brain organoids, and 2) evaluation of selected and novel compounds for anti-inflammatory potential and/or other adverse effects for treatment options for multiple sclerosis, and 3) Epigenetics focuses on turning on and off these genes may affect the certain processes.
Additional affiliations
May 2001 - July 2002
Oregon State University
Position
  • Visiting Scientist
October 1997 - October 2016
Pamukkale University
Position
  • Instructor
September 1997 - present
Pamukkale University
Position
  • Primary Investigator & Instructor
Education
September 1991 - January 1997
Middle East Technical University
Field of study
  • Biochemistry
September 1989 - August 1991
September 1984 - July 1989

Publications

Publications (107)
Article
Full-text available
Capparis ovata is a natural plant that grows widely in Turkey and its flowering buds and berry pickle are used in traditional medicine. Thus, the current study was expanded to evaluate the biochemical, pharmacological, and toxicological aspects of the Capparis ovata water extract (COWE). To determine the biochemical properties of COWE, mineral and...
Article
In this study, cerium oxide nanoparticles (CONPs) were synthesized using fresh green walnut shell extract in microwave environment. The morphology and structure of the CONPs were determined using ultraviolet‐visible (UV‐Vis), attenuated total reflection‐Fourier transform infrared (ATR‐FTIR), X‐ray diffraction (XRD), energy‐dispersive X‐ray (EDX) sp...
Article
Full-text available
OBJECTIVE Defects in apoptotic cell death which restrict the success of conventional cytotoxic therapies have pivotal roles in a number of pathological conditions including cancer. However, a novel drug class targeting pro-survival Bcl-2 protein family members has been developed with the understanding of the structures and interactions of Bcl-2 pro...
Article
Full-text available
Background and objective: This study was aimed to elucidate the molecular mechanism of Momordica charantia (MCh), along with a standard drug prednisolone, in a rat model of colitis induced by trinitrobenzene sulfonic acid (TNBS). Methods: After the induction of the experimental colitis, the animals were treated with MCh (4 g/kg/day) for 14 conse...
Article
Full-text available
Context Capparis L. (Capparaceae) is grown worldwide. Caper has been used in traditional medicine to treat various diseases including rheumatism, kidney, liver, stomach, as well as headache and toothache. Objective To isolate and elucidate of the secondary metabolites of the C. ovata extracts which are responsible for their anti-inflammatory activ...
Article
Full-text available
Oleanolic acid (OA) and its derivatives are widely found in diverse plants and are naturally effective pentacyclic triterpenoid compounds with broad prophylactic and therapeutic roles in various diseases such as ulcerative colitis, multiple sclerosis, metabolic disorders, diabetes, hepatitis and different cancers. This review assembles and presents...
Article
Mesalamine [5-aminosalicylic acid (5-ASA)] is a substantial supportive agent in the treatment of inflammatory bowel diseases (IBD), particularly in ulcerative colitis (UC). It is well known that 5-ASA is metabolized by phase II enzymes. And most likely cytochrome P450 enzymes have an important role in this process. However, there is no information...
Article
Full-text available
Cytochromes P450s (CYPs) are terminal enzymes in CYP dependent monooxygenases, which constitute a superfamily of enzymes catalysing the metabolism of both endogenous and exogenous substances. One of their main tasks is to facilitate the excretion of these substances and eliminate their toxicities in most phase 1 reactions. Endogenous substrates of...
Article
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This research was designed to determine about the possible potency of medicinal plants Viscum album L. and its major polyphenolic ingredient, o-coumaric acid on rat liver cholesterol and bile acid metabolizing enzymes. Bioactive compounds of V. album extracts were identified using LC-MS technique. The water extracts of V. album (10 mg/kg) and o-cou...
Article
Full-text available
The phytochemical compounds are used mostly in medicine as alternative medicine. Excessive cholesterol is removed mainly through conversion to bile acids in mammals, cytochromes P450 initiate all quantitatively significant pathways of cholesterol metabolism and bile acid biosynthesis. This study was aimed to investigate the possible potency of medi...
Article
Full-text available
In this study we report that amino acridine intermediates 7 and 8 were obtained from the reduction of nitro acridine derivatives 5 and 6 that were synthesized via the condensation of dimedone, p-nitrobenzaldehyde with various amine derivatives, respectively. Then acridine sulfonamide hybrid compounds (9–18) were synthesized by the reaction of amino...
Article
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Cladribine (2-CdA) is used as an anti-cancer drug but is currently studied as a potential treatment for use in relapsing-remitting multiple sclerosis (MS). In this study, we computer designed, synthesized, and characterized two novel derivatives of 2-CdA, K1–5d and K2–4c, and investigated their underlying mechanism of beneficial effect using the CC...
Article
Full-text available
Multiple sclerosis (MS) is an autoimmune disease of the human central nervous system. It is one of the most common neurological disorders around the world and there is still no complete cure for MS. Purification of a terpenoid from Capparis ovata was carried out and its structure was elucidated as stigmast-5,22-dien-3β-ol, myristate (3β,22E-stigmas...
Article
Full-text available
A rhizosphere isolate Streptomyces sp. CAH29 was found to possess potent antibacterial and antifungal activity against a variety of test organisms. Based on 16S ribosomal ribonucleic acid sequence homology studies, this strain was found to be similar to Streptomyces stramineus (gene sequence similarity 99 %). The major bioactive metabolite produced...
Article
Multiple Sclerosis (MS) is a very common disease of vital importance. In the MS treatment, some drugs such as fingolimod which help to protect nerves from damage are used. The main goal of the drug therapy in MS is to take control of the inflammation which leads to the destruction of myelin and axons in nerve cell and thus prevent and stop the prog...
Article
Since ancient times, capparis species have been widely used in traditional medicine to treat various diseases. Our recent investigations suggested Capparis ovata’s potential anti-neuroinflammatory application for the treatment of multiple sclerosis (MS). The present study was designed to determine its underlying mechanism of its anti-neuroinflammat...
Article
Full-text available
Plants have developed mechanisms to defend themselves against many factors including biotic stress such as herbivores and pathogens. Glutathione S-transferase (GST) is a glutathione-dependent detoxifying enzyme and plays critical roles in stress tolerance and detoxification metabolism in plants. Pinus brutia Ten. is a prominent native forest tree s...
Article
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In the last decade, hydroxycinnamic acids (HCA) have gained increasing attention from researchers due to their antioxidant potential. The aim of this study was to examine in detail the impact of dietary HCA on particular types of P450 and also selected phase II and antioxidant enzymes in Wistar rat. HCA (10 mM/kg/day, i.p.) was administered for ten...
Article
This study was performed to elucidate the molecular mechanisms of Capparis ovata extract on experimental animal model of multiple sclerosis. Capparis ovata water extract was prepared as described (Turkish Patent Institute, PT 2012/04 093). Animal MS model, experimental allergic encephalomyelitis (EAE) was induced in 6-8 weeks old C57BL/6 mice. MSCo...
Article
This study was performed to elucidate the molecular mechanisms of Capparis ovata extract on experimental animal model of multiple sclerosis. Capparis ovata water extract was prepared as described (Turkish Patent Institute, PT 2012/04 093). Animal MS model, experimental allergic encephalomyelitis (EAE) was induced in 6-8 weeks old C57BL/6 mice. MSCo...
Article
ABSTRACT In the present study, the possible role of ellagic acid (EA) on antioxidant potential of Epilobium hirsutum (EH) in rat liver was investigated. Wistar rats were intraperitoneally treated with 37.5 mg/kg of EH and 10 mg/kg of EA for 9 days. Effects of EH and EA on antioxidant [glutathione peroxidase (GPx) and superoxide dismutases (SOD)] an...
Article
In this study, we aimed to investigate the extent of genotoxic risk and the association between null GSTM1/GSTT1 and GSTP1 Ile105Val variants and cellular DNA damage, as measured by micronucleus (MN) assay in a group of agricultural workers from Denizli, Turkey. Peripheral blood samples were collected from 116 subjects, including 58 workers who wer...
Article
Mitochondrial DNA sequences from cytochrome c oxidase subunit I (COI) were used to provide a phylogeny of the Chironomidae (Diptera) from Turkey. Data were obtained from 70 species of Chironomidae belonging to the genera Ablabesmyia, Chironomus, Cladotanytarsus, Conchapelopia, Cricotopus, Cryptochironomus, Dicrotendipes, Endochironomus, Eukiefferie...
Article
Although humans are exposed to o-coumaric acid (OCA) in their diet, there is no available literature related to drug interaction and the carcinogen-activating potential of OCA in the HepG2 cell line. This study was undertaken to determine the effects of OCA on the cytochrome P450 (CYP) 1A2, CYP2E1, CYP2C9, and CYP3A4 enzymes, which are primarily in...
Article
Epilobium hirsutum (EH) is a medicinal plant for treating various diseases. Despite its wide usage, there is no available information about its potential influences on drug metabolism. The present study was undertaken to determine the in vivo effects of EH on hepatic CYP2B, CYP2C, CYP2D, and CYP3A enzymes that are primarily involved in drug metabol...
Article
Cytochrome P450 monooxygenases mediate a broad range of oxidative reactions involved in the biosynthesis of both primary and secondary metabolites in plants. Until now, only two P450 genes, CYP720B1 from Pinus taeda and CYP720B4 from Picea sitchensis, have been functionally characterised and described in the literature. The purpose of this study wa...
Article
Full-text available
Aromatase catalyzes the conversion of androgens into estrogens. Data on native aromatase enzyme kinetics and thus actual catalytic activity are scarce in fish, impeding comparison of aromatase activity (AA) from different organs within and between species. In the present study, fluorescence aromatase assay was optimized to measure AA in the gilthea...
Article
Full-text available
Objective: In this study we have investigated the effect of extracts obtained from well-known vegetable diets consumed commonly in Mediterranean and Aegean regions of Turkey on the expression of aromatase at protein, mRNA and activity levels in human non-small cell lung cancer cells- PC-3 and PC-14 cell lines. Method: For this purpose, cytotoxicity...
Article
Among hydroxycinnamic acids, caffeic, ferulic and p-coumaric acids have received considerable attention due to their biological activities. However, studies related to the biological activities of o-coumaric acid (OCA) are limited. In this regard, this study was designed to determine the chemopreventive potential of OCA in human breast cancer cells...
Article
Full-text available
The present study was designed to evaluate different doses of ellagic acid (EA) in vivo in rats for its potential to modulate hepatic phases I, II, and antioxidant enzymes. EA (10 or 30 mg/kg/day, intragastrically) was administered for 14 consecutive days, and activity, protein, and mRNA levels were determined. Although the cytochrome P450 (CYP) 2B...
Article
Full-text available
The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 1.8.3.1) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinking water. First, hepatic SOX activity in deficient...
Conference Paper
Viscum album L., a species of mistletoe, contains lectins, polypeptides, mucilage, sugar alcohols, flavonoids, lignans, triterpenes, and phenylallyl alcohols1. The leaves and twigs of Viscum album L., taken as tea, have been traditionally used for hypertension, stomachache, diarrhea, dysuria and also as analgesic and cardiotonic agent in Anatolia,...
Conference Paper
kserdar1@yahoo.com Abstract Epilobium hirsutum L. (E.H) known as its high number of phenolic and flavonoid contents is distributed in worldwide. As a medicinal herb it has been used as an anticoagulant, anti-inflammatory and rectal and menstrual bleeding preventer. CYP2B1, CYP2C6, CYP2D6 and CYP3A1 are the important isoforms of cytochrome P450 e...
Conference Paper
Epilobium hirsutum L. is found in moist waste ground of the Mediterranean region, Europe, Asia, and Africa. It shows analgesic, anti-microbial and anti-proliferative activity, and it is used in our country as an alternative medicine. The pharmacological effect of Epilobium hirsutum L. could be explained by the presence of polyphenolics including st...
Conference Paper
The aim of the present study was to evaluate the in vivo effects of the lyophilised water extract from combination of flowering buds, flowers and fruit of Capparis ovata on the expression of drug metabolizing enzymes in mice. Caper parts were supplied by Asci Murat Capers, Ice Cream, Dessert and Pickle Manufacturing & Export Co., Ltd. Caper has bee...
Conference Paper
Coumaric acids are hydroxy derivatives of cinnamic acid. There are three isomers, o-coumaric acid, m-coumaric acid, and p-coumaric acid that differ by the position of the hydroxy substitution of the phenyl group. o-Coumaric acid (trans-2-Hydroxycinnamic acid) is found in vegetables, fruits, coffee, tea and nuts and it is one of the common phenolic...
Conference Paper
Pesticides are one of the most widely used chemicals all over world. Many of them are classified as toxic and carcinogenic compounds thus representing a great threat for both environment and exposed individuals. Farmers are densely exposed to these chemicals by the way of inhalation and direct skin contact during the preparation and applications, a...
Article
Full-text available
Humans are exposed to acrylamide in their diet and cigarette smoke. Acrylamide is metabolized into glycidamide by CYP2E1. However, very few studies regarding the effects of acrylamide on cytochrome P450 and Glutathione S-Transferase (GST) isozymes have been pursued. The aim of this study is to elucidate the effects of acrylamide on cytochrome P450...
Article
Cholinesterase (ChE) and ethoxyresorufin-O-deethylase (EROD) were of special interest to this study as these biochemical tools have been widely used for the determination of exposure to pollutants. In this study, the freshwater oligochaete Limnodrilus profundicola was tested for its potential as a bioindicator of freshwater pollution. For this purp...
Article
Full-text available
This study was undertaken to determine the possibility of potential impact of PAHs on the aquatic biota. First, we had developed a new method for measuring 13 different priority pollutant PAHs in waste water samples. Then, eight different water samples collected from different sites along the Menderes River were analysed by this method involving SP...
Article
Full-text available
The modulatory effects of the Cyclamen trochopterantum tuber extract on hepatic drug-metabolizing enzymes, including aniline 4-hydroxylase (A4H; CYP2E1), ethoxyresorufin O-deethylase (EROD; CYP1A), methoxyresorufin O-demethylase (MROD; CYP1A), caffeine N-demethylase (C3ND; CYP1A2) aminopyrene N-demethylase (APND; CYP2C6), and erythromycin N-demethy...
Conference Paper
Cyclamen trochopterantum is one of the endemic species of cyclamens that grows naturally in the south-western part of Turkey. It is used as an ethno-medicine for treatment of hemorrhoids, infertility and eczema and expelling digestive tract worms. It is well established that extracts prepared from different cyclamen species show interesting spermic...
Conference Paper
Recent studies have implicated that estrogen and estrogen receptors play essential role in some lung cancers progression. It has been demonstrated that this steroidal growth-stimulatory effect may be promoted by tumor expression and activity of aromatase (CYP19), an estrogen synthase, in some lung cancers. Therefore, in this study we have investiga...