Alaa Kareem NiamahUniversity of Basrah · College of Agriculture
Alaa Kareem Niamah
Professor
Full professor of bacteriocin production, Lactic acid bacteria, Probiotic bacteria and dairy microbiology
About
98
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Introduction
I am a professor at the College of Agriculture, University of Basrah, Basra, Iraq, and my primary responsibilities involve teaching undergraduate and graduate students in the field of food science. My expertise lies in dairy microbiology, fermented milk, dairy technology, probiotic bacteria, food biotechnology, natural antimicrobials, essential oils, and related subjects. I have a significant publication record, including peer-reviewed papers, technical articles, book chapters, and books.
Additional affiliations
September 2017 - present
June 2013 - November 2016
September 2002 - November 2016
Education
September 2006 - April 2010
Publications
Publications (98)
Modern cancer diagnostics and treatment options have greatly improved survival rates; the illness remains a major cause of mortality worldwide. Current treatments for cancer, such as chemotherapy, is not cancer-specific and may cause harm to healthy cells; therefore, it is imperative that new drugs for cancer be developed that are both safe and eff...
Nephrolithiasis is a medical condition characterized by the existence or development of calculi, commonly referred to as stones within the renal system, and poses significant health challenges. Calcium phosphate and calcium oxalate are the predominant constituents of renal calculi and are introduced into the human body primarily via dietary sources...
Immunomodulation capability and cytotoxicity activities of novel exopolysaccharides produced by a new local strain, Bifidobacterium longum subsp. infantis strain Iraq-Basrah 3 Exopolysaccharides (EPS), metabolic products secreted by lactic acid bacteria (LAB) strains, have several biomedical effects. Our study focused on the extraction and purifica...
Metabolic products of exopolysaccharide (EPS) generated by LAB strains have several biomedical effects. Our study focused on the
isolation of a new strain of Bifidobacterium spp. From the sources of children's feces, examining them to choose the best EPS-producing strain, studying all the optimal conditions to increase the efficiency of producing...
Lactic acid bacteria are a significant bioproduct of exopolysaccharides (EPS) in lactic acid. EPS is popular in the food and dairy industries due to its ability to lengthen shelf
life, improve technical functionalities and provide a range of health benefits. The potential of EPS in drug development and diagnostics is also overwhelming. This study...
Enzymes have been a part of human society from prehistoric times, even before history was written. Numerous tradi-
tional foods, including bread, cheese, wine, and beer, have been prepared with the help of enzymes (Verma et al.,
2018). The modern period makes extensive use of enzymes, particularly in the food processing industry, in order to pro-
d...
Honey is a natural product that is used by a large number of people because of its distinctive
compositional constituents, which have a considerable impact on its market value. The distinctive
combination of amino acids and sugars found in honey’s composition, along with its peptide content,
could potentially provide several benefits to human healt...
The aim of this study was to compare the physical, chemical, and microbiological features of bio-yoghurt that had been air-oven-dried at three temperatures (40, 50, and 60 • C) to those of fresh bio-yoghurt. The results showed that drying bio-yoghurt at 40-60 • C decreased the number of probiotic starter bacteria in dried yoghurt products compared...
Aroma compounds are key components of food, and the food industry places a high priority on the enhancement of these
chemicals. Streptococcus thermophilus (ST), Lactobacillus bulgaricus (LB), Limosilactobacillus reuteri (LR), Lactobacillus
acidophilus (LA-5), Bifidobacterium bifidum (BB-12) and Saccharomyces boulardii (SB) were mixed starter cultur...
Because of the positive effects that probiotics have on human health, their use in pharmaceutical formulations and the development of functional foods have become increasingly widespread. The use of functional foods, such as probiotic foods, has become a mainstream trend in modern dietetics. To be effective, the health benefits of probiotic foods r...
The significance of consuming an adequate amount of nutrients on a consistent basis has a significant bearing on the preservation of the body's core physiological processes as well as its metabolism. In recent years, there has been a lot of focus placed on oils, fats, and fatty acids as a result of the critical role that consuming a diet rich in nu...
The milk of mammals contains a disaccharide called lactose, which is often commonly referred to as milk sugar. Lactose does not have an overwhelming sweetness, has weak solubility, and cannot be absorbed straight from the gastrointestinal system. Lactase is an enzyme that breaks down lactose into glucose and galactose. Glucose and galactose have a...
Reuterin was anaerobically manufactured by developing Lactobacillus reuteri LBIQ1 (a local isolate) at 37 °C in the MRS broth medium.
Cell biomass was harvested after 18 hours of incubation and used to ferment 278 mMol glycerol for 3 hours in a water-glycerol solution
at 37 °C. Cells were then extracted from the suspension solution, which remained...
This study aimed to investigate the impact of adding Zahdi date juice on the physicochemical characteristics and microbiological analysis of bio-yogurt with a probiotic starter (St. thermophilus, Lb. acidophilus, and B. longum) and haematological parameters in mice. The bioyogurt was prepared by adding a 5% probiotic starter supplemented with date...
Biopolymers, particularly exopolysaccharides produced by microorganisms such as bacteria, yeasts, and algae, have gained popularity in recent years due to their physical, chemical, and functional properties that are widely useful in food, industrial, cosmetic, and pharmaceutical systems. Hyaluronic acid is one type of these polysaccharides. The aim...
The present study aimed to extract β-glucan from Saccharomyces cerevisiae for use as a fortification at different ratios (0, 0.1, 0.5, and 1%) on the growth of three bio-yoghurt starters (Lactobacillus acidophilus, Bifidobacterium bifidum and Streptococcus thermophilus). The properties of bio-yoghurts were also studied, along with the effect of fee...
Emerging evidence shows the trend of using safe and natural preservatives like bacteriocins in food processing. The increasing demand by the food industry to extend keeping quality and prevent spoilage of various food products has appealed for new preservatives and new methods of conservation. Further, to discover the novel spectrum of antimicrobia...
The clinical and food testing microbiological laboratories required very rapid and careful methods for microbial identification and antibiotic sensitivity testing of microorganisms on regular basis. The VITEK II compact system is employed to identify microorganisms particularly bacteria, yeast, and molds automatically. The catalogs to identify VITE...
Purpose
The purpose of this study was to assess the bacterial viability, antioxidative activity, antimutagenicity and sensory evaluation of fermented onion types by using probiotic starters after fermentation at 37 °C for 24 hours and storage in the refrigerator for 28 days.
Design/methodology/approach
For onion fermentation, Lactobacillus acidoph...
The synthesis of hyaluronic acid (HA) by bacteria is a promising alternative to extracting the biopolymer from animal tissues. The production of HA by Streptococcus thermophilus, which belongs to the group of organisms generally recognized as safe (GRAS), is a well-studied viable alternative. The present study investigated five S. thermophilus stra...
Aroma and flavour represent the key components of food that improves the organoleptic characteristics of food and enhances the acceptability of food to consumers. Commercial manufacturing of aromatic and flavouring compounds is from the industry's microbial source, but since time immemorial, its concept has been behind human practices. The interest...
Lactic acid was isolated by using microbial fermentation in whey media which was carried out by local strain Lactobacillus paraplantarum then polymerized using the acidic medium’s polycondensation method. FTIR and H-NMR were used to characterize the isolated lactic acid monomer and polyalctic acid (PLA), and the results confirmed the chemical struc...
The use of agricultural pesticides is very important in improving production, but the residue of these pesticides on crops have a serious health effect on humans. On the other hand, there is another type of pollutants resulting from the use of veterinary medicines, as well as heavy metals and mycotoxins that also result in health risks to humans du...
In recent decades, there has been a lot of interest in the development of probiotic incorporated functional foods. It is because probiotic bacteria give various health advantages when ingested in sufficient quantities. However, the stability and viability of probiotics in various food products that can be employed as carriers are restricted by inhe...
Pesticide residue has become as one of the major food adulterants as a result , rapid, simple, spectrophotometric method for
pesticide residue measurement (bifenthrin, glyphosate) in milk sample based on UV-Vis spectrophotometer and Artificial
neural network had been developed .The developed system detecting the milk contaminants like pesticide res...
Microbial fermentation have several advantages to produce pure lactic acid (LA) by using cheap material. Whey is a byproduct of cheese industry utilized as fermentation media with local isolate L.paraplantarum. LA diagnosed with high performance liquid chromatography (HPLC), Nuclear magnetic resonance (NMR) and fourier transform infrared spectrosco...
A local bacterial isolate from fecal samples of infants 1-3 months, previously isolated and identified as Lactobacillus reuteri, was used for reuterin production and characterization then applying it in local soft cheese preservation. Reuterin was produced under anaerobic conditions by growing these bacteria in the MRS broth medium at 37°C. After 1...
A total number of 20 samples were collected from different local Iraqi sources. The samples were diluted and inoculated on MRS agar medium. After growth, the isolates were transferred on the modified MRS selective agar medium in order to get isolate of Lactobacillus reuteri which able the reuterin production. Five isolates were obtained from the hu...
A variety of organisms produce bioactive peptides that express inhibition activity against other organisms. Saccharomyces cerevisiae is considered the best example of a unicellular organism that is useful for studying peptide production. In this study, an antibacterial peptide was produced and isolated from Saccharomyces cerevisiae (Baker's yeast)...
A variety of organisms produce bioactive peptides that express inhibition activity against other organisms. Saccharomyces cerevisiae is considered the best example of a unicellular organism that is useful for studying peptide production. In this study, an antibacterial peptide was produced and isolated from Saccharomyces cerevisiae (Baker's yeast)...
Curdlan - a homopolysaccharide is comprised of glucose using β-1,3-glycosidic bond and produced by different types of microorganisms as exopolysaccharide. Curdlan gel is stable during freezing and thawing processes which find several applications in food and pharmaceutical industries. It acts as a prebiotic, stabilizer and water-holding, viscosifyi...
The present study was aimed to test optimum conditions for the levan production from local isolation Bacillus subtilis subsp. subtilis w36, which was isolated from Basrah city soil by using production medium containing (40 g of sucrose as carbon source 10g pepton, 1g (NH4) 2SO4, 1g KH2PO4, 1g MgSO4.7H2O). The amount of levan produced was 4.9 g.ml-1...
This book, Microbiology for Food and Health: Technological Developments and Advances highlights the innovative microbiological approaches and advances made in the field of microbial food industries. The volume covers the most recent progress in the field of dairy and food microbiology, emphasizing the current progress, actual challenges, and succes...
The effect of ultrasonic treatment at 40 kHz for 0, 5, 10, 15 and 20 minutes on the growth of five different strains of probiotic bacteria (Lactobacillus acidophilus LA-5, Lactobacillus casei LC, Lactobacillus reuteri LR-MM53, Bifidobacterium bifidum Bb-12 and Bifidobacterium loungm BB-536) in fermented milk was investigated. The study findings ind...
Talk about Mendeley program and writing correct of references in manuscript or thesis
The effect of microencapsulation by alginate and alginate-chitosan on the survival of Saccharomyces boulardii ATCC MYA796
in simulated gastric and intestinal juices was investigated. The survival of yeast microencapsulated cells treated in ice cream production. The percentage survival of alginate and alginate-chitosan encapsulated cells treated in...
Saccharomyces boulardii was used for antimicrobial peptides production. Separation process of produced antimicrobial peptides was conducted using ultrafiltration technique through dialysis membranes with porous 10 (MWCO) kDa. The inhibition activity was determined against four bacterial isolates. As a result, higher inhibition zone against Bacillus...
Pediocin is a low molecular weight (2.7-17 KDa) cationic molecules
compose of a hydrophilic N-terminal part containing the pediocin box (YGNGV) motif
and a hydrophobic or amphiphilic C-terminal variable part which is produced by some
of Pediococcus bacteria. Pediocin has inhibition properties against several sensitive
bacterial cells and acts on th...
Pediocin is a low molecular weight (2.7-17 KDa) cationic molecules compose of a hydrophilic N-terminal part containing the pediocin box (YGNGV) motif and a hydrophobic or amphiphilic C-terminal variable part which is produced by some of Pediococcus bacteria. Pediocin has inhibition properties against several sensitive bacterial cells and acts on th...
This study was carried out in the food science department, Agriculture College, Basrah University to investigate on the effect of different chitosan concentration on drinking water quality. The studied parameters were turbidity, TDS, electrical conductivity and pH. The results showed that the turbidity, TDS, electrical conductivity and pH have been...
This study was carried out in the Food Science Department, Agriculture College, Basrah University to investigate the effect of different chitosan concentrations on drinking water quality. The studied parameters were turbidity, TDS, electrical conductivity and pH. The results showed that the turbidity, TDS, electrical conductivity and pH have been d...
The chemical composition of fermented soy milk by probiotic starter which consist of Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacterium sp. were studied. The percentage of each moisture, protein, fat, ash and carbohydrate were (88.8, 4.2, 2.3, 0.82 and 4.6) respectively. The cold storage effect for 28 days were studied for probi...
This study aimed to identify the essential oil compound from Cinnamomum zeylanicum barks by using GC-MS analysis and evaluate essential oil inhibition effects against Listeria monocytogenes, Escherichia coli, Enterobacter aerogenes, Pseudomonas erogenous and Staphylococcus aureus by using agar diffusion method and studied kill-time for this bacteri...
Four strains of the dry yeast Saccharomyces cerevisiae were obtained from different sources. The identification of yeasts was confirmed by morphological and physiological properties. Mannan extracts from yeast cell walls ranged between 25.80-34.40% of the dry weight of yeast cells. Five different concentrations of mannan extract were mixed with buf...
Bioactive peptide was used to extend period of storage for ground beef
tablets with different concentrations (0.5%, 1%, 1.5% and 0.2%). It found that
there was reducing in bacterial numbers compared with control sample during
storage time (15 days) in cool condition . Also, the total number of bacteria
was low at 2% concentration of peptide. In add...
The effect of adding Saccharomyces boulardii on yogurt quality was studied. Yogurt control was made using whole cow’s milk and classic starter culture. Other three treatments of yogurt were made by adding 1%,2% and 3% of Saccharomyces boulardii with yogurt starter. pH values and proteolytic activity of all yogurt treatments were determined during f...
The chemical composition of soy milk fermented by probiotic starter (Log.11.1 CFU/ml) which consists of Streptococcus thermophilus, Lactobacillus acidophilus LA-5, and Bifidobacterium bifidum Bb-12 was studied. The percentage of each moisture, protein, fat, ash, carbohydrate and total acidity were 88.8, 4.2, 2.3, 0.82, 4.6, and 0.83, respectively,...
Saccharomyces boulardii إل القراص الخزني العمر طالة (مختلفة وبتراكيز المفروم البقري اللحم 1،5.0.0،5.2 %) إ , األ في انخفاض لوحظ ذ البكتيري عداد ة عينة مع ً مقارنة ل بالتبريد الخزن عند السيطرة لمدة من 0 إلى 05 يوم ً ا للبكتريا الكلي العدد في الزيادة كانت و الهوائية بمعد...
This study aimed to identify the essential oil compound from Cinnamomum zeylanicum barks by using GC-MS analysis and evaluate essential oil inhibition effects against Listeria monocytogenes, Escherichia coli, Enterobacter aerogenes, Pseudomonas erogenous and Staphylococcus aureus by using agar diffusion method and studied kill-time for this bacteri...
The current study was carried out for testing the inhibitory spectrum of the metabolic extract of Saccharomyces boulardii yeast against (26) bacterial isolates. The metabolic extract of Saccharomyces boulardii yeast was obtained after growth in YEPG medium and centrifuged. The broad inhibitory spectrum of yeast extract against Gram-positive bacteri...
Saccharomyces boulardii was used for antimicrobial peptides production. Separation process of produced antimicrobial peptides was conducted using ultrafiltration technique through dialysis membranes with porous 10 (MWCO) kDa. The inhibition activity was determined against four bacterial isolates. As a result, higher inhibition zone against Bacillus...
The optimal condition of production yogurt from soy milk was the starter supplied from Chr.Hensan Company which consist of Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacterium sp. were used in soybean milk fermentation and the best incubation temperature was 40 °C for 7 hours , and inoculation volume 5%. The log of the total count...
he first section addresses advances in microorganism research, which present threats and benefits to abundant, healthy food, and associated environments. The constant spread and evolution of agricultural pathogens provides a continually renewed source of challenges to productivity and food safety. Pathogens continue to cause harm once food has left...
The current study includes soymilk extraction from its grains with
three different extraction methods and study the chemical composition,
physical properties of the milk for each method. the second method was
chosen because its produce milk includes 10.44% total solid material, the
chemical composition consist of moisture 89.56%, protein 4.188%, fa...
This chapter discusses principles and applications of innovative and eco-friendly ultrasonic technology for treatment of milk and milk products.
This study focuses on extraction of essential oils from Iraqi seeds of Coriander plant (Coriandrum sativum L.). The yield of
essential oils was 2 % (V: W) of seed, analyzed by the GC- mass technique. The essential oil contained 35 varieties of compounds, with
Linalool (59.14 %) compound prevailing in the essential oils extract. The essential oils o...
Five different concentrations of Gum Arabic (GA) added to yogurt product by probiotic culture starter, which contained Lactobacillus acidophilus, Bifidobacterium bifidum and Streptococcus thermophilus. Best concentration of GA was added 1%. The chemical composition of yogurt with 1% GA was (86.97, 3.88, 3.21, 0.79 and 5.15) % for moisture, protein,...
The current study included the preparation of the aqueous and alcohol extract of leaves of Ziziphus (Ziziphus spina-christi) and Eucalyptus plant (Eucalyptus camaldulensis) and assessed its antimicrobial activity against reference strains of bacteria and mold. The chemical compound detection Ziziphus leaves results showed that the aqueous extract a...
Utilization of high intensity ultrasound in food technology and biotechnology is in the sense of this progressive technology usage on most usable processes in food industry. Those include mixing, drying,homogenization, crystallization, grinding and many other operations. High intensity ultrasound is capable to change physical and chemical character...
Abstract
An experiment was carried out to study the role of some organic acids (acetic acids, citric
acids and boric acids) with concentrations of 2%, 4% and 6% for every acids on storability of
tomato fruits cv. Super Marmande grown in Basrah. Results showed that tomato fruits could
be stored for 21 days at 5±2ºc, after packing in polyethylene bag...
In this study, Bioactive compounds of six solvent different extracts, from the dry yellow banana peels were present. Methanol was the best solvent extraction .The yield extract, total phenolic content and flavonoids concentration were 1.96 g, 81.89 mg G.A./g and 55.44mg RU/g from extract. The FT-IR spectra were recorded pronounced peaks belonging t...
Lactobacillus acidophilus bacteria was isolated by using a new method, 8 isolates of L. acidophilus obtained by using identification test.
Probiotic starter was prepared by mixing L. acidophilus, Bifidobacterium bifidum and Streptococcus thermophilus at ratio (1:1:1).
Standard starter contained Lactococcus lactis subsp lactis,Lactococcus lactis sub...
T he enzyme activity for five fungal isolates from Aspergillus niger
growth on a solid substrate was studied and it was found that best isolate ( A5 ) .
The optimum conditions for the enzyme production was studied. It was found
that the enzyme activity ( 2.8 u/ml ) was obtained at PH 5 as compared with
these pH ( 3 , 4 , 6 and 7 ) . while that opti...
In this study, detection of prf A gene of Listeria monocytogenes bacteria in four types of milk (cow, buffalo, camel and sheep) electrophoresis was showed products of gene amplification clear bunds at 274 bp. In all samples inoculated with bacteria after 0 h and 24 h. The numbers of bacteria ranging from 44x10 -55x10 cfu/ml directly after inoculati...
The present study was undertaken to prepared chitosan by chemical method from shrimp shell and effect it's on the qualities of apple juice including chemical, microbiological and sensory qualities were examined , The results showed of addition of the chitosan as a clarifying agent was 0.4g/l at 25±2 Cº and 60min statistical analysis's showed that n...
A 62 isolates were isolated from 33 samples of shrimp and peeled shrimp in three
local markets in Basrah city. A 36 isolates were identified to be Aeromonas hydrophila
bacteria depending on morphological, microscopic examinations and ten biochemical tests.
83.33% (30 isolates) from isolates produced ß-haemolysis on tryptone soy agar with 5%
sheep b...
The effect of ultrasonic treat ment on milk ho mogenization and numbers o f bacterial were studied. The results showed that ultrasound treatments with high power had an important effect on milk ho mogenizat ion. The lowest homogenizat ion indices (high efficiency) were 2 and 3% at 430 and 338W respectivily. The homogenization index was reduced with...
The study showed that the addition of bacterial extract to meat and cheese reducing the bacterial numbers. The numbers increased after 14 days, but did not reach the setup at the beginning of the experiment and this reduction increased with increasing bacterial extract concentration in the samples. Staphylococci was found to be the most affected ba...
Trichoderma harzianum pour isolate was obtained from Al-Tahaddi insecticide , It was best growth on Malt extract agar medium show spores numbers 8066.6 spore /disc (0.3 cm) and colony diameter was 9 cm. It was best growth on cellulase production medium contained 100% substitution level from palm leaves, spores numbers was 3900 spore/disc (0.3 cm) a...