Alaa Hani Al-Charrakh

University of Babylon · Department of Microbiology

The present study aimed detecting and characterizing of β-lactamases producing E.cloacae isolated from different clinical sources in Hilla hospitals using phenotypic and molecular methods. A total of 308 samples were collected from two major hospitals at Hilla Province from October 2013 to April 2014. All isolates were tested biochemically, it was...

Background The risk of developing a more aggressive disease condition is influenced by age and the presence of comorbidities such as diabetes, hypertension, obesity, and cardiovascular diseases. Diabetics may experience late diabetes complications such as diabetic renal disease and ischemic heart disease, which can make them frailer and exacerbate...

Background One of the efflux pump inhibitors is carbonyl cyanide 3-chlorophenylhydrazone (CCCP) that has often been found to increase the susceptibility of a number of multi-drug resistant (MDR) MDR bacteria, isolated from human clinical specimens. Objectives To investigate the role of active efflux system to aminoglycoside and quinolones resistan...

This case-control study aimed to investigate active immunization strategies using flagellin purified from Pseudomonas aeruginosa to provide complete protection against extensive drug resistance (XDR) Pseudomonas aeruginosa acute pulmonary infection in the rat model. After Pseudomonas aeruginosa isolates were identified, the animals were immunized i...

Background Evaluating immune responses following COVID-19 vaccination is paramount to understanding vaccine effectiveness and optimizing public health interventions. This study seeks to elucidate individuals’ immune status after administering a second dose of diverse COVID-19 vaccines. By analyzing immune responses through serological markers, we a...

Background Evaluating immune responses following COVID-19 vaccination is paramount to understanding vaccine effectiveness and optimizing public health interventions. This study seeks to elucidate individuals’ immune status after administering a second dose of diverse COVID-19 vaccines. By analyzing immune responses through serological markers, we a...

Background: Hepatitis B virus (HBV) continues to be a global public health problem, especially following the spread of SARS-CoV-2 and the prevalence of hepatic manifestations of COVID-19 in individuals with HBV. Objectives: The current work aims to investigate HBV genetic sequences by analysis of S genes of selected patients for HBV alone and HBV w...

Background Empagliflozin (EMP) is an oral anti-diabetic drug with pleiotropic effects such as anti-inflammatory properties. Objectives To evaluate the antidepressant effect of EMP and describe the link between stress and interleukin-6 (IL-6) level in the brains of male rats. Materials and Methods In this experiment, 50 rats were separated into fi...

Background Empagliflozin is an antidiabetic medication having anti-inflammatory and antioxidant properties. Objectives To develop a chronic unpredictable stress (CUS) model in male rats, evaluate empagliflozin’s antidepressant effects, and describe the link between stress, an antioxidant enzyme, and Toll-like receptor (TLR)-4 gene expression in ma...

Background The emergence of multidrug-resistant Escherichia coli is a major public health threat worldwide. Objectives This study was conducted to determine the sensitivity pattern and class 1 integron of E. coli isolated from various clinical sources in Babylon, Iraq. Materials and Methods A total of 1874 clinical samples were collected from pat...

Pseudomonas aeruginosa had been studied widely due chronically invade and infect many patients especially those immuno-compromised such as patients with malignant or HIV infection. Also, increase the prevalence of MDR P. aeruginosa infection especially those chronic pulmonary infection that lead to increasing of morbidity and mortality rate. This s...

Background and Objectives: Escherichia coli is well known to be a universal commensal flora in humans as well as in several animal species. Antimicrobial resistance (AMR) has emerged as one of the principal public health problems of the 21st century. This study was undertaken for detection of the antibiotic resistance and Amp C β-lactamase genes fo...

Background Hydatidosis is caused by infection with the larval stage of the Cestode Echinococcus granulosus . Objective In vitro quantitative evaluation of interleukin 6 (IL-6), INF-gamma (INF-γ), and CD4 and CD8 molecules during hydatidosis infection and control group. Materials and Methods In vitro quantitative determination of IL-6, INF-γ, and...

Background Hepatic manifestations of COVID-19 are prevalent in individuals infected with viral hepatitis type B (HBV). Objectives The current study aims to determine the extent of the HBV reactivation depending on the immune impact on patients infected with SARS-CoV-2. Materials and Methods One hundred forty-one hospitalized cases were divided in...

Infections caused by Third-Generation Cephalosporins (3GC)-resistant Enterobacteriaceae were significantly associated with the increased hazard of death and excess length of stay and costs. A total of 266 blood samples were collected from adult patients with bacteremia admitted to Medical City in Baghdad. The antimicrobial susceptibility was conduc...

The great catastrophe that the world faced after World War II, which is the Corona epidemic, was discovered in the 2019 year in Hubei Province, Wuhan, China, and it is a severe respiratory disorder. The World Health Organization gave it this name, and it is one of the viruses (SARS-CO-2). Among the problems that the world faces is how to control th...

Objective: This study was carried out to evaluate the prevalence of HBV, HCV, and HIV among blood donors, and to identify some features of seropositive blood donors‏‏ in the Main blood bank in Najaf province, Iraq. Method: This cross-sectional study was carried out among blood donors. Data were collected from the records of the Main blood bank. Scr...

Infection with the hepatitis B virus can lead to asymptomatic carrier status, developing into severe chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Ten genotypes (A-J) and 46 sub-genotypes have been discovered. As a result, the current study was conducted to determine HBV genotypes in Iraqi patients. RT-PCR was used to determine the hi...

The emergence of severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) has presented a formidable medical challenge to health systems and clinicians. This study was designed to investigate the incidence of βhaemolytic streptococci in patients with COVID-19 and study their antimicrobial susceptibility patterns. A total of 378 throat swab sampl...

The purpose of this rapid review is to investigate the development of the hepatic manifestations and challenges with SARS-COV-2 and multiple viral hepatitis infections. The literature on the most critical viral, genetic and immunological factors between SARS-CoV-2 and viral hepatitis was reviewed, and the most important interactions that occur with...

Background: Due to their importance in vaccination, we tried in this study to purify the flagellin type a and type b separately from P. aeruginosa by a simple and cheap method with acceptable purity for preclinical use. Methods: Specific primer was used to detect the type a and b flagellin gene (fliC gene) in P. aeruginosa clinical isolates. Flagel...

Background: Multi drug resistance (MDR) P. aeruginosa consider the main cause of morbidity in hospitalize patients suffering from chronic airway infections such as chronic pulmonary disease, pneumonia and cystic fibrosis (CF). Also, the rapidly development of antibiotic resistance to the last generations of a broad spectrum antibiotic were detected...

This study was designed to investigate the incidence of β-haemolytic streptococci in patients with COVID-19 and study their antimicrobial susceptibility patterns. A total of 388 clinical samples were collected from Covid-19 patients who suffering from acute pharyngitis. β-hemolytic isolates were diagnosed using the Vitek 2 system. Streptex agglutin...

Methicillin-resistant Staphylococcus aureus (MRSA) is in charge of the common nosocomial infections and is capable of evading the biocidal action of several antimicrobial agents. The objective of this study was to detect isolates of S. aureus carrying mecA and capsular polysaccharides (CP5) gene in Iraq. A total of 180 different clinical samples we...

Keywords: ABSTRACT Capsular polysaccharides (CP5), MRSA, Purification; UV-visible spectrophotometry. Methicillin-resistant Staphylococcus aureus (MRSA) is the common cause of nosocomial infections and is capable of evading the biocidal action of several antimicrobial agents. The objective of this study was for the isolation and partial purification...

Purpose This cross sectional study was designed to investigate the molecular detection of C. perfringens toxins (alpha, epsilon, iota) and antibiotics resistance genes, as well as sequencing of their toxin genes. Methods Different wound swabs were taken from 140 patients. PCR was applied for detection of clostridial toxins; alpha toxin (cpa) gene,...

Acknowledgment From the journal Journal of Basic and Clinical Physiology and Pharmacology https://doi.org/10.1515/jbcpp-2021-2002

Background: ESKAPE pathogens are responsible for the common of nosocomial infections and capable of 'escaping' the biocidal action of antimicrobial agents. Aims: The aim of this study is to screen the prevalence of ESKAPE pathogens group among pediatric patients in Iraq. Methods: A total of 191 different clinical samples were collected from pediatr...

Background: Identification of coagulase-negative staphylococci (CoNS) as nosocomial pathogens or contaminants is significant for microbiologists and clinicians. Aims: This study aimed to determine the frequency of isolation and antimicrobial resistance patterns of CoNS isolates from nosocomial bloodstream infections (BSIs). Methods: This studyinclu...

This certificate has been awarded to Prof. Alaa H. Al-Charrakh, In recognition of participating in the online Lecture entitled “New trend in vaccination against human eczema / atopic dermatitis” Which was held on 5/9/2020, within the activities of Jabir Bin Hayyan International Forum for Science.

Certificate of Attendance of International Webinar on "COVID-19 ... The hard road" on 27th June 2020 held by Aliraqia University, College of Dentistry.

Certificate for Alaa H. Al-Charrakh for _International WEBINAR on “Better understanding of pandemic COVID-19" on 23rd June 2020, India.

A certificate of ‬participation in an International webinar on "Pathophysiology of SARS-COV2 and possible target drug therapy" on 24th Apr 2020, held by Coll. of Medicine /University of Babylon, in cooperation with Shri B. Patil Medical College/ BLDE University, India.

A certificate of ‬participation in an International webinar on "Pathophysiology of SARS-COV2 and possible target drug therapy" on 24th Apr 2020, held by Coll. of Medicine /University of Babylon, in cooperation with Shri B. Patil Medical College/ BLDE University, India.

New approach for genotyping uropathogenic E.coli using multiplex PCR assay

Nanobiotechnology is a new emerging discipline of nanoscience created by pairing of biotechnology, and nanotechnology. Silver nitrate (AgNO3) was used as precursor for biosynthesis of silver nanoparticles by Streptomyces spp.Silver nitrate was added with concentration 5 mMto biomass of Streptomyces spp. (MU-43 &SA-65)which distributed in sterilized...

Background: Suicide is a substantial public health concern and one of the commonest cause of death throughout the world. In Iraq, the suicide rate is a current health problem exacerbated by the fact that it is a country suffered war and destruction in all fields of life. Objectives: We tested whether Tumor necrosis factor-alpha, Interlukin-1β, and...

The current study was conducted to determine carbapenemase-producing Acinetobacter baumannii clinical isolates recovered from two hospitals in Iraq. A total of 540 different clinical samples were collected. Isolates were identified and Carbapenemase production were detected using MBL E-test, Modified Hodge's Test (MHT), and Modified Carba NP test....

The current study was conducted to determine carbapenemase-producing Acinetobacter baumannii clinical isolates recovered from two hospitals in Iraq. A total of 540 different clinical samples were collected. Isolates were identified and Carbapenemase production were detected using MBL E-test, Modified Hodge's Test (MHT), and Modified Carba NP test....

Apr 2019Indian Journal of Public Health Research and Development

Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of Rotavirus (RV) among children with acute diarrhea. One hundred a...

Objective: This study was carried out to evaluate the prevalence of HBV, HCV, and HIV among blood donors, and to identify some features of seropositive blood donors in the Main blood bank in Najaf province, Iraq. Methods: This cross-sectional study was carried out among blood donors. Data were collected from the records of the Main blood bank. Scr...

Clostridium perfringens is a major cause of gas gangrene. The morbidity of C. perfringens is connected with producing toxins. This cross-sectional study was designed to isolate, genetically diagnose, and study the antibiotic susceptibility patterns of C. perfringens isolated from clinical samples. Different wound swabs (from diabetic patients, cell...

Clostridium perfringens is a major cause of gas gangrene. The morbidity of C. perfringens is connected with producing toxins. This cross-sectional study was designed to isolate, genetically diagnose, and study the antibiotic susceptibility patterns of C. perfringens isolated from clinical samples. Different wound swabs (from diabetic patients, cell...

This cross-sectional study was conducted to investigate antibiotic resistance and virulence factors in different Gram negative bacteria associated with keratitis and conjunctivitis. Eye swabs specimens were collected from patients having conjunctivitis or keratitis. Gram negative bacterial isolates recovered from conjunctivitis and keratitis were...

The aim of this study was to determine the genotypic and antifungal susceptibility of C. albicans obtained from cancer patients. The study included fifty cancer patients treated with chemotherapy who exhibited evident oral lesions. Oral swabs from cancer patients and healthy persons screened for the occurrence of C. albicans. Isolates were identifi...

The aim of this study was to determine the genotypic and antifungal susceptibility of C. albicans obtained from cancer patients. The study included fifty cancer patients treated with chemotherapy who exhibited evident oral lesions. Oral swabs from cancer patients and healthy persons screened for the occurrence of C. albicans. Isolates were identifi...

Aspergillus infections are among the most feared opportunistic infections in humans because they are capable of causing several distinct pulmonary diseases. The aim of this study was to determine the prevalence of Aspergillus species in patients with pulmonary pneumonia and susceptibility testing to the most commonly used antifungal agents. A total...

Aim This study was designed to investigate the occurrence of serotypes of Listeria monocytogenes, an important food-borne pathogen, in gallbladder samples from cattle and sheep. Materials and Methods Three hundred samples were collected and screened for the presence of L. monocytogenes. The identification of the isolates was confirmed by API-Liste...

Rotaviruses are classified in the genus Rotavirus and belong to family Reoviridae is a family of viruses that can affect the gastrointestinal system. Viruses in the family Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA). Bacteroides fragilis (B. fragilis) is an anaerobic gram-negative bacillus causes diarrheal disease b...

This cross sectional and hospital-based study was done to investigate the prevalence of ESKAPE pathogens group among pediatric patients in Babylon province, Iraq. At the beginning of the study, 191 different clinical samples were collected from pediatric patients during the a period of July 2016 till November 2016. Samples were obtained from pedia...

The genus Aeromonas comprises important human pathogens causing primary and secondary septicemia in immunocompromised patients, serious wound infections in healthy individuals and in patients undergoing medicinal leech therapy. Gastroenteritis, the most common clinical manifestation, remains controversial. The genus is made up of psychrophiles and...

Background: Metallo beta lactamase(MBL) producing Pseudomonas aeruginosahave been reported to be an important nosocomialinfections.Pseudomonas aeruginosa is a leading cause of nosocomial infections, giving risetoa wide range of life-threatening conditions. Its intrinsic & acquired resistance to many antimicrobial agents and its ability to developmu...

A total of 140 samples from wound sites were taken from patients who admitted to two main hospitals in Hilla city, Iraq (Hilla Teaching Hospital and Medical Marjan City), during the period from February to October 2016. Swabs were collected from diabetic patients, anaerobic cellulitis, and bullet wounds. These wound swab samples were subjected to d...

Objective: The aim of this study was to investigate the prevalence of tetracycline-resistant Aeromonas hydrophila isolated from clinical sources in Iraq, using polymerase chain reaction (PCR) technique. Materials and Methods: A total of 822 samples were collected from patients suffering from diarrhea. Isolates were identified using biochemical test...

The aim of this study was to investigate genes encoding virulence factors of E. faecalis isolated from patients with significant bacteriuria in Najaf, Iraq. Two hundred and ten mid-stream urine samples were collected from patients suspected of UTI and screened for the occurrence of E. faecalis. The presence of genes encoding virulence factors, incl...

The aim of this study was to determine the genotypic and antifungal susceptibility of C. albicans obtained from cancer patients. The study included fifty cancer patients treated with chemotherapy who exhibited evident oral lesions. Oral swabs from cancer patients and healthy persons screened for the occurrence of C. albicans. Isolates were identifi...

A total of 1200 specimens were collected from human and animals. 600 specimens from patients suffering from diarrhea who were referred to Al-Diwaniya Teaching Hospital for Maternity and Children. 600 specimens from gall bladder (bile) of cattle from Al-Diwaniya slaughter house. This study was conducted during the period that extended from May 2013...

This study was carried out in October 2015-March 2016. A total of 182 samples of non-sterile medications labeled with manufacturing and expiration dates were collected from different retailer drug stores placed within the city of Hilla (Babylon province). The contamination of non-sterile pharmaceuticals (NSPs) was not only found to cause spoilage o...

The aim of this study was to investigate the correlation between biofilm production and heavy metals resistance (HM) in P. aeruginosa isolated from clinical samples. Forty three P. aeruginosa isolates were tested for their susceptibility to lead, copper, and mercury using agar pouring method. Biofilm production of P. aeruginosa isolates were also i...

This study aimed to determine the incidence of human respiratory syncytial virus (hRSV) and metapneumovirus (hMPV) occurrence in asthmatic patients. Blood samples were collected for measure the total and differential WBCs. ELISA has been applied for detection of total-IgE. Results revealed that the highest total-IgE antibodies titer in sera were si...

Psoriasis is a common, immune mediated, heterogeneous, multifactorial disease. The relationships of serum IFNγ, IL-17 and IL-4 levels and Staphylococcus aureus superantigens to severity of psoriasis in patients were evaluated. A total of 60 patients with mild – severe psoriasis and 60 healthy individuals (controls) were enrolled. These patients att...

This study aimed to determine the incidence of human respiratory syncytial virus (hRSV) and metapneumovirus (hMPV) occurrence in asthmatic patients in Wasit province. Blood samples were collected for measure the total and differential white blood cells count (WBCs). Correlation results of total and differential WBCs count for neutrophils, lymphocyt...

This seminar talking about the main criteria that used for Determining Predatory Open-Access Publishers and Journals. Every researcher (Professor or postgraduate student) must be careful of publishing online open access journals.

Metallo-β-lactamase (MBL) producing Pseudomonas aeruginosa has been reported to be an important nosocomial infection. Its intrinsic and acquired resistance to various antimicrobial agents and its ability to develop multidrug resistance imposes a serious therapeutic problem. Different clinical samples were collected from public and private hospitals...

Background: The use of selective and differential plating media is a simple method for the isolation of Salmonella spp. Recently, there has been a general move toward molecular methods of Salmonella detection and typing. Methods: A total of 1200 different specimens collected from human and animal sources were involved in his study. 600 stool spe...

2015 December 8 2 epted Acc Abstract Streptomyces are the biggest source for antibiotics production, they constitute about more than 75% in antibiotic productivity, and these antibiotics have a significant application in the commercial and therapeutic fields. The study included screening on the most potent antibacterial producing Streptomyces isola...

Streptomyces are the biggest source for antibiotics production, they constitute about more than 75% in antibiotic productivity, and these antibiotics have a significant application in the commercial and therapeutic fields. The study included screening on the most potent antibacterial producing Streptomyces isolates. 41 Streptomyces isolates were sc...

Aeromonas hydrophila is distributed widely in nature and is responsible for an array of human infections. Several studies on the isolation and characterisation of the organism abound. Although there are reports on the antibiotic resistance profiles of the organism. The aim of this study was to investigate the prevalence of tetracycline resistance g...

The aim of this study was to determine the prevalence of A. fumigatus in patients with pulmonary pneumonia, and molecular characterization of these fungal isolates obtained from clinical specimens. This aim was achieved using the following objectives: 1- Studying the prevalence of pulmonary pneumonia caused by Aspergillus spp. in both immunocompete...

This study aimed to determine the role and incidence of some respiratory viruses such as respiratory syncytial virus and metapneumovirus diagnostic from nasopharyngeal swabs associated with asthmatic patients in the Wasit province, Iraq. A total of 80 samples were taken from patients suffered from exacerbations asthma, who were admitted to Al-Karra...

The aim of this study was to determine the prevalence of A. fumigatus isolates in patients with pulmonary pneumonia and molecular characterization of these fungal isolates. A total 311 specimens were collected from patients presented with pulmonary complain. Out of which 139 (44.6%) yielded Aspergillus growth. Six patients (4.31%) were presented w...

A total of 1200 different specimens collected from human and animals sources. 600 specimens from patients suffering from diarrhea who were admitted to Al-Dewaniya Teaching Hospital for Maternity and Children. 600 specimens from gall bladder (bile) of cattle from Al-Diwaniya slaughter house. This study was conducted during the period from May 2013 t...

P. aeruginosa is a gram-negative aerobe bacteria. It commonly found in the environment, at any moist location. It is a common cause of nosocomial infections in humans. Severe immunodeficiencies and medical devices predispose the patients to P. aeruginosa infections.

Salmonella comprises of about 2,500 serotypes. It is the parasite of intestine of poultry and animals (cattles) S. typhi and paratyphi – human are the only hosts. Diseases caused by salmonella include: 1. Gastroenteritis - S. typhimurium 2. Enteric fever - S. typhi (typhoid fever) - S. paratyphi A,B,C (paratyphoid fevers) 3. Bacteremia -mostly S. c...

Shigella is a Gram-negative, non-motile rods, non lactose fermenters. It is not a member of normal colonic flora. Transmission from person to person by “Four Fs” fingers, flies, food, faeces. It causes Shigellosis (dysentery). It is only a human disease.

H. pylori: a spiral-shaped, gram-negative rod. It is highly motile with polar flagella. It can grow on complex media in microaerophilic (5% O2 and 10% CO2 ) conditions. H. pylori is associated with gastritis, peptic ulcer, gastric adenocarcinoma, gastric mucosa-associated lymphoid type (MALT) B-cell lymphomas. Chronic gastritis is a risk factor for...